AU2002211719B2 - Compositions and methods for using a solid support to purify RNA - Google Patents

Compositions and methods for using a solid support to purify RNA Download PDF

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Publication number
AU2002211719B2
AU2002211719B2 AU2002211719A AU2002211719A AU2002211719B2 AU 2002211719 B2 AU2002211719 B2 AU 2002211719B2 AU 2002211719 A AU2002211719 A AU 2002211719A AU 2002211719 A AU2002211719 A AU 2002211719A AU 2002211719 B2 AU2002211719 B2 AU 2002211719B2
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AU
Australia
Prior art keywords
rna
solid support
solution
biological material
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2002211719A
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English (en)
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AU2002211719A1 (en
Inventor
Ellen M. Heath
John M. Wages, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
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Qiagen North American Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings Inc filed Critical Qiagen North American Holdings Inc
Publication of AU2002211719A1 publication Critical patent/AU2002211719A1/en
Priority to AU2004233035A priority Critical patent/AU2004233035B2/en
Assigned to QIAGEN NORTH AMERICAN HOLDINGS, INC. reassignment QIAGEN NORTH AMERICAN HOLDINGS, INC. Request for Assignment Assignors: GENTRA SYSTEMS, INC.
Application granted granted Critical
Publication of AU2002211719B2 publication Critical patent/AU2002211719B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002211719A 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify RNA Expired AU2002211719B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2004233035A AU2004233035B2 (en) 2001-10-12 2004-04-15 Compositions and methods for using a solid support to purify RNA

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/032073 WO2003033739A1 (en) 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify rna

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2004233035A Division AU2004233035B2 (en) 2001-10-12 2004-04-15 Compositions and methods for using a solid support to purify RNA

Publications (2)

Publication Number Publication Date
AU2002211719A1 AU2002211719A1 (en) 2003-07-03
AU2002211719B2 true AU2002211719B2 (en) 2007-06-14

Family

ID=21742909

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002211719A Expired AU2002211719B2 (en) 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify RNA

Country Status (5)

Country Link
EP (1) EP1438426A1 (enExample)
JP (1) JP3979996B2 (enExample)
AU (1) AU2002211719B2 (enExample)
CA (1) CA2463317A1 (enExample)
WO (1) WO2003033739A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002333673B2 (en) * 2001-11-06 2008-08-14 Promega Corporation Isolation and purification of nucleic acids

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2304017C (en) 1997-09-17 2007-05-01 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
ATE449186T1 (de) 2001-11-28 2009-12-15 Applied Biosystems Llc Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP3983125B2 (ja) 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
US7115719B2 (en) 2003-12-16 2006-10-03 Gentra Systems, Inc. Formulations and methods for denaturing proteins
WO2005093065A1 (en) * 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Improved method of isolating nucleic acids
US20060105372A1 (en) 2004-11-05 2006-05-18 Bair Robert J Compositions and methods for purifying nucleic acids from stabilization reagents
JP4956727B2 (ja) 2005-08-30 2012-06-20 倉敷紡績株式会社 Ｒｎａ分離精製方法
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
US9442046B2 (en) 2011-06-19 2016-09-13 Abogen, Inc. Device for sample collection
EA201591229A1 (ru) 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
KR102392407B1 (ko) 2014-03-07 2022-04-29 디엔에이 제노텍 인코퍼레이티드 생물학적 샘플에서 핵산을 안정화하기 위한 조성물 및 방법
EP4729528A3 (en) 2014-04-25 2026-06-24 Translate Bio, Inc. Methods for purification of messenger rna
US11174500B2 (en) 2018-08-24 2021-11-16 Translate Bio, Inc. Methods for purification of messenger RNA

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
DE59506735D1 (de) * 1994-06-14 1999-10-07 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002333673B2 (en) * 2001-11-06 2008-08-14 Promega Corporation Isolation and purification of nucleic acids

Also Published As

Publication number Publication date
JP2005505305A (ja) 2005-02-24
WO2003033739A1 (en) 2003-04-24
CA2463317A1 (en) 2003-04-24
JP3979996B2 (ja) 2007-09-19
EP1438426A1 (en) 2004-07-21

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Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: QIAGEN NORTH AMERICAN HOLDINGS, INC.

Free format text: FORMER APPLICANT(S): GENTRA SYSTEMS, INC.

MK14 Patent ceased section 143(a) (annual fees not paid) or expired