CA2463317A1 - Compositions and methods for using a solid support to purify rna - Google Patents

Compositions and methods for using a solid support to purify rna Download PDF

Info

Publication number
CA2463317A1
CA2463317A1 CA002463317A CA2463317A CA2463317A1 CA 2463317 A1 CA2463317 A1 CA 2463317A1 CA 002463317 A CA002463317 A CA 002463317A CA 2463317 A CA2463317 A CA 2463317A CA 2463317 A1 CA2463317 A1 CA 2463317A1
Authority
CA
Canada
Prior art keywords
rna
solid support
solution
biological material
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002463317A
Other languages
English (en)
French (fr)
Inventor
Ellen M. Heath
John M. Wages, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2463317A1 publication Critical patent/CA2463317A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002463317A 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify rna Abandoned CA2463317A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/032073 WO2003033739A1 (en) 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify rna

Publications (1)

Publication Number Publication Date
CA2463317A1 true CA2463317A1 (en) 2003-04-24

Family

ID=21742909

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002463317A Abandoned CA2463317A1 (en) 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify rna

Country Status (5)

Country Link
EP (1) EP1438426A1 (enExample)
JP (1) JP3979996B2 (enExample)
AU (1) AU2002211719B2 (enExample)
CA (1) CA2463317A1 (enExample)
WO (1) WO2003033739A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1027145A4 (en) 1997-09-17 2004-08-25 Gentra Systems Inc APPARATUS AND METHODS FOR ISOLATING NUCLEIC ACID
US7148343B2 (en) 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
EP1442045B1 (en) * 2001-11-06 2009-01-07 Cortex Biochem Inc. Isolation and purification of nucleic acids
DE60234464D1 (de) 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP3983125B2 (ja) * 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
EP1694692B1 (en) * 2003-12-16 2010-04-28 QIAGEN North American Holdings, Inc. Formulations and methods for denaturing proteins
WO2005093065A1 (en) * 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Improved method of isolating nucleic acids
KR20070097430A (ko) 2004-11-05 2007-10-04 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
JP4956727B2 (ja) 2005-08-30 2012-06-20 倉敷紡績株式会社 Ｒｎａ分離精製方法
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
BR112013032629A2 (pt) 2011-06-19 2017-08-01 Abogen Inc dispositivos, soluções e métodos para coletamento de amostra
EP2970955B1 (en) * 2013-03-14 2018-11-14 Translate Bio, Inc. Methods for purification of messenger rna
DK3114225T3 (da) 2014-03-07 2021-07-26 Dna Genotek Inc Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver
SG11201608725YA (en) 2014-04-25 2016-11-29 Shire Human Genetic Therapies Methods for purification of messenger rna
CN112930396B (zh) 2018-08-24 2024-05-24 川斯勒佰尔公司 用于纯化信使rna的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
EP0765335B1 (de) * 1994-06-14 1999-09-01 Invitek GmbH Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis

Also Published As

Publication number Publication date
WO2003033739A1 (en) 2003-04-24
EP1438426A1 (en) 2004-07-21
JP2005505305A (ja) 2005-02-24
JP3979996B2 (ja) 2007-09-19
AU2002211719B2 (en) 2007-06-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued