CA2463317A1 - Compositions et procedes utilises en tant que support solide afin de purifier de l'arn - Google Patents

Compositions et procedes utilises en tant que support solide afin de purifier de l'arn Download PDF

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Publication number
CA2463317A1
CA2463317A1 CA002463317A CA2463317A CA2463317A1 CA 2463317 A1 CA2463317 A1 CA 2463317A1 CA 002463317 A CA002463317 A CA 002463317A CA 2463317 A CA2463317 A CA 2463317A CA 2463317 A1 CA2463317 A1 CA 2463317A1
Authority
CA
Canada
Prior art keywords
rna
solid support
solution
biological material
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002463317A
Other languages
English (en)
Inventor
Ellen M. Heath
John M. Wages, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2463317A1 publication Critical patent/CA2463317A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Abstract

L'invention concerne des réactifs, des procédés et des nécessaires destinés à la purification de l'ARN des matériaux biologiques.
CA002463317A 2001-10-12 2001-10-12 Compositions et procedes utilises en tant que support solide afin de purifier de l'arn Abandoned CA2463317A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/032073 WO2003033739A1 (fr) 2001-10-12 2001-10-12 Compositions et procedes utilises en tant que support solide afin de purifier de l'arn

Publications (1)

Publication Number Publication Date
CA2463317A1 true CA2463317A1 (fr) 2003-04-24

Family

ID=21742909

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002463317A Abandoned CA2463317A1 (fr) 2001-10-12 2001-10-12 Compositions et procedes utilises en tant que support solide afin de purifier de l'arn

Country Status (5)

Country Link
EP (1) EP1438426A1 (fr)
JP (1) JP3979996B2 (fr)
AU (1) AU2002211719B2 (fr)
CA (1) CA2463317A1 (fr)
WO (1) WO2003033739A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1027145A4 (fr) 1997-09-17 2004-08-25 Gentra Systems Inc Appareil et procedes permettant d'isoler un acide nucleique
US7148343B2 (en) 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
ATE491790T1 (de) 2001-11-06 2011-01-15 Promega Corp Isolierung und reinigung von nucleinsäuren
DE60234464D1 (de) 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP3983125B2 (ja) * 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
WO2005058933A1 (fr) 2003-12-16 2005-06-30 Gentra Systems, Inc. Preparations et methodes de denaturation de proteines
WO2005093065A1 (fr) * 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Methode amelioree d'isolement d'acides nucleiques
CA2586532C (fr) * 2004-11-05 2014-03-25 Qiagen North American Holdings, Inc. Compositions et procedes pour purifier des acides nucleiques provenant de reactifs de stabilisation
JP4956727B2 (ja) 2005-08-30 2012-06-20 倉敷紡績株式会社 Ｒｎａ分離精製方法
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
EP2721140B1 (fr) 2011-06-19 2016-11-23 Abogen, Inc. Dispositifs, solutions et procédés de recueillement d'échantillons
EA201591229A1 (ru) * 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
DK3114225T3 (da) 2014-03-07 2021-07-26 Dna Genotek Inc Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver
EA201691696A1 (ru) 2014-04-25 2017-03-31 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
CA3108544A1 (fr) 2018-08-24 2020-02-27 Translate Bio, Inc. Procedes de purification d'arn messager

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
WO1994018156A1 (fr) * 1993-02-01 1994-08-18 University Of Iowa Research Foundation Agents tensioactifs amines quarternaires et procedes d'utilisation de ceux-ci
ATE184013T1 (de) * 1994-06-14 1999-09-15 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis

Also Published As

Publication number Publication date
JP3979996B2 (ja) 2007-09-19
WO2003033739A1 (fr) 2003-04-24
JP2005505305A (ja) 2005-02-24
EP1438426A1 (fr) 2004-07-21
AU2002211719B2 (en) 2007-06-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued