CA2319691C - Reactifs d'elution, procedes et trousses permettant d'isoler un adn - Google Patents

Reactifs d'elution, procedes et trousses permettant d'isoler un adn Download PDF

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Publication number
CA2319691C
CA2319691C CA2319691A CA2319691A CA2319691C CA 2319691 C CA2319691 C CA 2319691C CA 2319691 A CA2319691 A CA 2319691A CA 2319691 A CA2319691 A CA 2319691A CA 2319691 C CA2319691 C CA 2319691C
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CA
Canada
Prior art keywords
dna
solid support
reagent
cells
eluting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2319691A
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English (en)
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CA2319691A1 (fr
Inventor
Ellen M. Heath
Ruth M. Shuman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
Original Assignee
Qiagen North American Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings Inc filed Critical Qiagen North American Holdings Inc
Publication of CA2319691A1 publication Critical patent/CA2319691A1/fr
Application granted granted Critical
Publication of CA2319691C publication Critical patent/CA2319691C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne des réactifs d'élution et des procédés permettant d'isoler l'ADN de matières biologiques.
CA2319691A 1998-02-02 1999-02-02 Reactifs d'elution, procedes et trousses permettant d'isoler un adn Expired - Lifetime CA2319691C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12641398P 1998-02-02 1998-02-02
US12642998P 1998-02-02 1998-02-02
US60/126,413 1998-02-02
US60/126,429 1998-02-02
PCT/US1999/002190 WO1999039010A1 (fr) 1998-02-02 1999-02-02 Reactifs d'elution, procedes et trousses permettant d'isoler un adn

Publications (2)

Publication Number Publication Date
CA2319691A1 CA2319691A1 (fr) 1999-08-05
CA2319691C true CA2319691C (fr) 2012-12-18

Family

ID=26824623

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2319691A Expired - Lifetime CA2319691C (fr) 1998-02-02 1999-02-02 Reactifs d'elution, procedes et trousses permettant d'isoler un adn

Country Status (5)

Country Link
EP (1) EP1053353A1 (fr)
JP (2) JP2004500002A (fr)
AU (1) AU2574299A (fr)
CA (1) CA2319691C (fr)
WO (1) WO1999039010A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516731A (ja) 1997-09-17 2001-10-02 ジエントラ・システムズ・インコーポレーテツド 核酸を単離するための装置および方法
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
EP1141234A4 (fr) * 1999-01-06 2004-05-19 Invitrogen Corp Procedes et compositions permettant d'isoler des molecules d'acide nucleique
WO2002005930A1 (fr) * 2000-07-18 2002-01-24 Invitrogen Corporation Dispositif et procedes de subdivision et de filtration de matiere de gel et d'extraction de molecules de celle-ci
AU2002226053A1 (en) 2000-12-12 2002-06-24 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
EP1466018B2 (fr) 2002-01-08 2015-08-12 Roche Diagnostics GmbH Utilisation d'un materiau de silice dans une reaction d'amplification
WO2004033470A2 (fr) * 2002-10-04 2004-04-22 Whatman, Inc. Methodes et matieres pour une utilisation de composes chimiques, en tant qu'outils de stockage d'acide nucleique sur un support de systemes de purification d'acide nucleique
JP2008220380A (ja) * 2003-10-31 2008-09-25 Fujifilm Corp 核酸の分離精製方法
EP1809744B1 (fr) * 2004-11-05 2018-06-06 QIAGEN North American Holdings, Inc. Compositions et procedes pour purifier des acides nucleiques provenant de reactifs de stabilisation
US9243289B2 (en) * 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
US20170342399A1 (en) * 2014-11-07 2017-11-30 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
EP3294912A4 (fr) * 2015-05-12 2019-01-09 Gen-Probe Incorporated Procédé de regroupement de prélèvements sanguins
MX2019010800A (es) 2017-03-15 2020-07-28 Ancestry Com Dna Llc Dispositivo y metodo de recoleccion de muestras.

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JPH04158784A (ja) * 1990-10-24 1992-06-01 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及びその利用
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
JPH06277040A (ja) * 1991-02-13 1994-10-04 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及び溶解法
JP3294288B2 (ja) * 1991-02-22 2002-06-24 明治乳業株式会社 乳酸桿菌由来の新規なプラスミドｐＢＵＬ１ 及びその誘導体
CA2067712A1 (fr) * 1991-05-03 1992-11-04 Daniel L. Woodard Purification par filtration de l'adn
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
EP0580305B1 (fr) * 1992-07-02 1999-09-22 Edge Biosystems, Inc. Procédé et matériau pour la purification d'acides nucléiques
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5438127A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US6180778B1 (en) * 1994-02-11 2001-01-30 Qiagen Gmbh Process for the separation of double-stranded/single-stranded nucleic acid structures
WO1995034569A1 (fr) * 1994-06-14 1995-12-21 Invitek Gmbh Procede universel d'isolement et de purification d'acides nucleiques a partir de quantites extremement reduites de differents materiaux de depart fortement contamines
CN1192648A (zh) * 1995-06-07 1998-09-09 弗林德斯科技有限公司 用于贮存和分析遗传物质的干固体介质
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
WO1999039009A1 (fr) * 1998-02-02 1999-08-05 Gentra Systems, Inc. Procedes permettant d'isoler, d'amplifier et de caracteriser un adn

Also Published As

Publication number Publication date
WO1999039010A1 (fr) 1999-08-05
EP1053353A1 (fr) 2000-11-22
CA2319691A1 (fr) 1999-08-05
AU2574299A (en) 1999-08-16
JP2012157366A (ja) 2012-08-23
JP2004500002A (ja) 2004-01-08

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Effective date: 20190204