JP2006516891A5 - - Google Patents

Info

Publication number

JP2006516891A5

JP2006516891A5 JP2006500210A JP2006500210A JP2006516891A5 JP 2006516891 A5 JP2006516891 A5 JP 2006516891A5 JP 2006500210 A JP2006500210 A JP 2006500210A JP 2006500210 A JP2006500210 A JP 2006500210A JP 2006516891 A5 JP2006516891 A5 JP 2006516891A5

Authority

JP

Japan

Prior art keywords

nucleic acid

light output

output intensity

sample

template nucleic

Prior art date

2004-01-14

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000000034 method Methods 0.000 claims 39
• 150000007523 nucleic acids Chemical class 0.000 claims 38
• 108020004707 nucleic acids Proteins 0.000 claims 37
• 102000039446 nucleic acids Human genes 0.000 claims 37
• 230000003321 amplification Effects 0.000 claims 20
• 238000003199 nucleic acid amplification method Methods 0.000 claims 20
• 238000006243 chemical reaction Methods 0.000 claims 16
• 108060001084 Luciferase Proteins 0.000 claims 5
• 239000005089 Luciferase Substances 0.000 claims 5
• 230000007423 decrease Effects 0.000 claims 5
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims 3
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims 3
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims 3
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims 3
• 238000003556 assay Methods 0.000 claims 3
• 238000005415 bioluminescence Methods 0.000 claims 3
• 230000029918 bioluminescence Effects 0.000 claims 3
• 102000004523 Sulfate Adenylyltransferase Human genes 0.000 claims 2
• 108010022348 Sulfate adenylyltransferase Proteins 0.000 claims 2
• 239000000203 mixture Substances 0.000 claims 2
• 239000000758 substrate Substances 0.000 claims 2
• IRLPACMLTUPBCL-KQYNXXCUSA-N 5'-adenylyl sulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OS(O)(=O)=O)[C@@H](O)[C@H]1O IRLPACMLTUPBCL-KQYNXXCUSA-N 0.000 claims 1
• FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
• 230000001351 cycling effect Effects 0.000 claims 1
• 238000003745 diagnosis Methods 0.000 claims 1
• MWEQTWJABOLLOS-UHFFFAOYSA-L disodium;[[[5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-oxidophosphoryl] hydrogen phosphate;trihydrate Chemical compound O.O.O.[Na+].[Na+].C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)C(O)C1O MWEQTWJABOLLOS-UHFFFAOYSA-L 0.000 claims 1
• 230000002068 genetic effect Effects 0.000 claims 1
• 229910052749 magnesium Inorganic materials 0.000 claims 1
• 239000011777 magnesium Substances 0.000 claims 1
• 244000052769 pathogen Species 0.000 claims 1
• 230000001717 pathogenic effect Effects 0.000 claims 1