CN1852990B - 确定样本中核酸数量的方法 - Google Patents

确定样本中核酸数量的方法 Download PDF

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Publication number
CN1852990B
CN1852990B CN2004800068992A CN200480006899A CN1852990B CN 1852990 B CN1852990 B CN 1852990B CN 2004800068992 A CN2004800068992 A CN 2004800068992A CN 200480006899 A CN200480006899 A CN 200480006899A CN 1852990 B CN1852990 B CN 1852990B
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China
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nucleic acid
sample
reaction
luciferase
amplification reaction
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CN2004800068992A
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Chinese (zh)
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CN1852990A (zh
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劳伦斯·C·第希
詹姆斯·A·H·默里
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Lumora Ltd
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Lumora Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2004800068992A 2003-01-14 2004-01-14 确定样本中核酸数量的方法 Expired - Lifetime CN1852990B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0300802.6A GB0300802D0 (en) 2003-01-14 2003-01-14 Method for detecting DNA polymerisation
GB0300802.6 2003-01-14
PCT/GB2004/000127 WO2004062338A2 (en) 2003-01-14 2004-01-14 Method for determining the amount of template nucleic acid present in a sample

Publications (2)

Publication Number Publication Date
CN1852990A CN1852990A (zh) 2006-10-25
CN1852990B true CN1852990B (zh) 2010-08-18

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CN2004800068992A Expired - Lifetime CN1852990B (zh) 2003-01-14 2004-01-14 确定样本中核酸数量的方法

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US (1) US7371545B2 (https=)
EP (1) EP1585836B1 (https=)
JP (1) JP4521581B2 (https=)
CN (1) CN1852990B (https=)
AT (1) ATE372392T1 (https=)
AU (1) AU2004204396B2 (https=)
CA (1) CA2513073C (https=)
CY (1) CY1107021T1 (https=)
DE (1) DE602004008740T2 (https=)
DK (1) DK1585836T3 (https=)
ES (1) ES2295815T3 (https=)
GB (1) GB0300802D0 (https=)
PT (1) PT1585836E (https=)
WO (1) WO2004062338A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002367744A1 (en) 2001-07-03 2003-10-27 The Board Of Trustees Of The Leland Stanford University Bioluminescence regenerative cycle (brc) for nucleic acid quantification
GB0300802D0 (en) 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
GB0516455D0 (en) * 2005-08-10 2005-09-14 Lumora Ltd pH tolerant luciferase
JP4782528B2 (ja) * 2005-10-04 2011-09-28 株式会社日立製作所 塩基配列決定方法と試薬
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
JP2010161935A (ja) * 2009-01-13 2010-07-29 Fujifilm Corp 核酸増幅反応におけるばらつきを低減する方法
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
EP3538668B8 (en) * 2016-11-10 2025-04-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of chlamydia trachomatis
CN106701966B (zh) * 2017-01-20 2020-12-11 东南大学 基于分析pcr副产物焦磷酸的病原微生物快速检测方法
US12325883B2 (en) 2019-01-15 2025-06-10 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection
US20200340038A1 (en) * 2019-04-25 2020-10-29 Nephros Inc. Portable System and Process Thereof to Rapidly Filter, Concentrate, and Detect Waterborne Pathogens
CN113853657A (zh) 2019-05-20 2021-12-28 3M创新有限公司 用于检测对生物测定的抑制的系统和方法
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
US11047007B1 (en) 2020-03-23 2021-06-29 Talis Biomedical Corporation Polynucleotides for amplification and detection of SARS-CoV-2
US20230332216A1 (en) * 2020-08-25 2023-10-19 Microgen Laboratories Methods and reagents for rapid detection of pathogens in biological samples

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2001042496A2 (en) * 1999-12-10 2001-06-14 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
US6258568B1 (en) * 1996-12-23 2001-07-10 Pyrosequencing Ab Method of sequencing DNA based on the detection of the release of pyrophosphate and enzymatic nucleotide degradation
WO2002064830A2 (en) * 2001-02-14 2002-08-22 Cambridge University Technical Services Limited Method for detecting dna polymerisation

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US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US20030104372A1 (en) * 1996-12-23 2003-06-05 Pyrosequencing Ab. Allele specific primer extension
WO1998055653A1 (en) 1997-06-06 1998-12-10 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
GB0021977D0 (en) * 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
JP2002306180A (ja) * 2001-04-16 2002-10-22 Hitachi Ltd 核酸塩基配列解析法および核酸塩基配列解析試薬キットおよび核酸塩基配列解析装置
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
AU2002367744A1 (en) 2001-07-03 2003-10-27 The Board Of Trustees Of The Leland Stanford University Bioluminescence regenerative cycle (brc) for nucleic acid quantification
US20040197793A1 (en) * 2002-08-30 2004-10-07 Arjang Hassibi Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing
GB0300802D0 (en) 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
EP1616027A4 (en) 2003-04-14 2009-07-22 Temasek Life Sciences Lab Ltd DETECTION OF TARGET NUCLEIC ACID BY MEANS OF POLYMERIC ACTION AND ENZYMATIC DETECTION OF UNLOCKED PYROPHOSPHATE

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258568B1 (en) * 1996-12-23 2001-07-10 Pyrosequencing Ab Method of sequencing DNA based on the detection of the release of pyrophosphate and enzymatic nucleotide degradation
WO2001042496A2 (en) * 1999-12-10 2001-06-14 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
WO2002064830A2 (en) * 2001-02-14 2002-08-22 Cambridge University Technical Services Limited Method for detecting dna polymerisation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HIGUCHI R ET AL:."KINETIC PCR ANALYSIS: REAL-TIME MONITORINGOF DNA AMPLIFICATION REACTIONS".BIO/TECHNOLOGY,11 9.1993,11(9),1026-1030. *
MORI YASUYOSHI ET AL:."Detection of loop-mediated isothermal amplificationreaction by turbidity derived from magnesiumpyrophosphate formation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,289 1.2001,289(1),150-154. *
NYGREN M ET AL:."QUANTIFICATION OF HIV-1 USING MULTIPLEQUANTITATIVE POLYMERASE CHAIN REACTIONSTANDARDS AND BIOLUMINOMETRIC DETECTION".ANALYTICAL BIOCHEMISTRY,288 1.2001,288(1),28-38. *
TABARY T ET AL:."HOMOGENEOUS PHASE PYROPHOSPHATE (PPI)MEASUREMENT (H3PIM). ?A NON-RADIOACTIVE,QUANTITATIVE DETECTION SYSTEM FOR NUCLEICACID SPECIFIC HYBRIDIZATION METHODOLOGIESINCLUDING GENE AMPLIFICATION".JOURNAL OF IMMUNOLOGICAL METHODS,156 1.1992,156(1),55-60. *
WHITE ET AL:."improved thermostability of the north american fireflyluciferase: saturation mutagenesis at position 354".BIOCHEMICAL JOURNAL,319.1996,319343-350. *

Also Published As

Publication number Publication date
ES2295815T3 (es) 2008-04-16
EP1585836A2 (en) 2005-10-19
AU2004204396B2 (en) 2010-06-03
WO2004062338A8 (en) 2005-10-06
EP1585836B1 (en) 2007-09-05
DE602004008740T2 (de) 2008-06-12
CA2513073A1 (en) 2004-07-29
WO2004062338A2 (en) 2004-07-29
CA2513073C (en) 2012-08-28
DE602004008740D1 (de) 2007-10-18
ATE372392T1 (de) 2007-09-15
CN1852990A (zh) 2006-10-25
US20060257874A1 (en) 2006-11-16
AU2004204396A1 (en) 2004-07-29
CY1107021T1 (el) 2012-09-26
PT1585836E (pt) 2007-12-14
JP2006516891A (ja) 2006-07-13
DK1585836T3 (da) 2008-01-02
US7371545B2 (en) 2008-05-13
GB0300802D0 (en) 2003-02-12
WO2004062338A3 (en) 2004-09-10
JP4521581B2 (ja) 2010-08-11

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