JP2006501836A - 検出システム - Google Patents
検出システム Download PDFInfo
- Publication number
- JP2006501836A JP2006501836A JP2004542659A JP2004542659A JP2006501836A JP 2006501836 A JP2006501836 A JP 2006501836A JP 2004542659 A JP2004542659 A JP 2004542659A JP 2004542659 A JP2004542659 A JP 2004542659A JP 2006501836 A JP2006501836 A JP 2006501836A
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- JP
- Japan
- Prior art keywords
- probe
- sample
- binding substance
- sequence
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title description 4
- 239000000523 sample Substances 0.000 claims abstract description 180
- 238000006243 chemical reaction Methods 0.000 claims abstract description 82
- 108020004414 DNA Proteins 0.000 claims abstract description 81
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- 238000000034 method Methods 0.000 claims abstract description 72
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 72
- 230000027455 binding Effects 0.000 claims abstract description 67
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- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 33
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 25
- 239000000203 mixture Substances 0.000 claims abstract description 23
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 18
- 238000012544 monitoring process Methods 0.000 claims abstract description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 12
- 230000001687 destabilization Effects 0.000 claims abstract description 4
- 238000003752 polymerase chain reaction Methods 0.000 claims description 35
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- KKZJGLLVHKMTCM-UHFFFAOYSA-N mitoxantrone Chemical group O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO KKZJGLLVHKMTCM-UHFFFAOYSA-N 0.000 claims description 23
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- 229960001156 mitoxantrone Drugs 0.000 claims description 18
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- WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 claims description 13
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 claims description 13
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- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical class Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 claims description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims description 5
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- 125000004433 nitrogen atom Chemical group N* 0.000 claims description 5
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- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 4
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- 108060002716 Exonuclease Proteins 0.000 claims description 3
- 125000002947 alkylene group Chemical group 0.000 claims description 3
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 claims description 3
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- 125000005843 halogen group Chemical group 0.000 claims description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 2
- 125000004423 acyloxy group Chemical group 0.000 claims description 2
- 125000003545 alkoxy group Chemical group 0.000 claims description 2
- 229940045799 anthracyclines and related substance Drugs 0.000 claims description 2
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- 125000004122 cyclic group Chemical group 0.000 claims description 2
- 125000004990 dihydroxyalkyl group Chemical group 0.000 claims description 2
- 125000000623 heterocyclic group Chemical group 0.000 claims description 2
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- 239000002773 nucleotide Substances 0.000 claims description 2
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 239000001022 rhodamine dye Substances 0.000 claims description 2
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical group N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
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- 108091093088 Amplicon Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
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- 238000004458 analytical method Methods 0.000 description 3
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- 229910052717 sulfur Inorganic materials 0.000 description 3
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 description 2
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- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
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- 239000002250 absorbent Substances 0.000 description 2
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- 125000001624 naphthyl group Chemical group 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
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- 238000001429 visible spectrum Methods 0.000 description 2
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- 101100243026 Arabidopsis thaliana PCO3 gene Proteins 0.000 description 1
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- JGLMVXWAHNTPRF-CMDGGOBGSA-N CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O Chemical compound CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O JGLMVXWAHNTPRF-CMDGGOBGSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000012625 DNA intercalator Substances 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical class O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- -1 Nogaramycin (2R- (2α Chemical class 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
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- 239000007983 Tris buffer Substances 0.000 description 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
- 125000005577 anthracene group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 125000004093 cyano group Chemical group *C#N 0.000 description 1
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- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
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- 239000012634 fragment Substances 0.000 description 1
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- 238000003780 insertion Methods 0.000 description 1
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- 239000003446 ligand Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 125000004043 oxo group Chemical group O=* 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
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- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
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- 238000005382 thermal cycling Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Eye Examination Apparatus (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Geophysics And Detection Of Objects (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0223563.8A GB0223563D0 (en) | 2002-10-10 | 2002-10-10 | Detection system |
| PCT/GB2003/004412 WO2004033726A1 (en) | 2002-10-10 | 2003-10-10 | Detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006501836A true JP2006501836A (ja) | 2006-01-19 |
| JP2006501836A5 JP2006501836A5 (enExample) | 2006-09-14 |
Family
ID=9945669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004542659A Pending JP2006501836A (ja) | 2002-10-10 | 2003-10-10 | 検出システム |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20060127906A1 (enExample) |
| EP (1) | EP1554404B1 (enExample) |
| JP (1) | JP2006501836A (enExample) |
| CN (1) | CN1723288A (enExample) |
| AT (1) | ATE429513T1 (enExample) |
| AU (1) | AU2003274316A1 (enExample) |
| CA (1) | CA2501636A1 (enExample) |
| DE (1) | DE60327345D1 (enExample) |
| GB (1) | GB0223563D0 (enExample) |
| WO (1) | WO2004033726A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009022512A1 (ja) * | 2007-08-14 | 2009-02-19 | Sony Corporation | 二本鎖核酸の形成に係る情報を得る方法 |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| WO2005059548A1 (ja) | 2003-12-19 | 2005-06-30 | Kankyo Engineering Co., Ltd. | 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ |
| US20070072211A1 (en) | 2005-06-30 | 2007-03-29 | Roche Molecular Systems, Inc. | Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids |
| GB0603190D0 (en) * | 2006-02-16 | 2006-03-29 | Enigma Diagnostics Ltd | Detection system |
| DK2004847T3 (en) | 2006-03-16 | 2017-08-21 | Pentabase Aps | Oligonucleotides comprising signaling pairs and hydrophobic nucleotides, "stemless beacons", for detection of nucleic acids, methylation status and mutants of nucleic acids |
| US8658366B2 (en) | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| GB201004339D0 (en) | 2010-03-16 | 2010-04-28 | Enigma Diagnostics Ltd | Sequence detection assay |
| GB201007868D0 (en) | 2010-05-11 | 2010-06-23 | Enigma Diagnostics Ltd | Sequence detection assay |
| EP2569447A4 (en) | 2010-05-14 | 2013-11-27 | Fluidigm Corp | ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| CN107002140A (zh) * | 2014-09-26 | 2017-08-01 | 双孔人公司 | 通过合成探针的纳米孔隙探测的靶序列检测 |
| US11486873B2 (en) | 2016-03-31 | 2022-11-01 | Ontera Inc. | Multipore determination of fractional abundance of polynucleotide sequences in a sample |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| DE102019219531A1 (de) * | 2019-12-13 | 2021-06-17 | Robert Bosch Gmbh | Verfahren zur Durchführung einer Amplifikationsreaktion in einer mikrofluidischen Vorrichtung |
| CN111562244B (zh) * | 2020-05-25 | 2023-02-07 | 赣南师范大学 | 一种检测柔红霉素的稀土时间分辨荧光探针和试剂盒 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0870876A (ja) * | 1994-09-01 | 1996-03-19 | F Hoffmann La Roche Ag | 発光性標識で標識されたオリゴヌクレオチドの分解検出方法 |
| WO2001092218A2 (en) * | 2000-05-31 | 2001-12-06 | Oridigm Corporation | Polyamine analogues as therapeutic and diagnostic agents |
| JP2004532044A (ja) * | 2001-05-25 | 2004-10-21 | イギリス国 | 検出システム |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1533151A (fr) * | 1962-05-18 | 1968-07-19 | Rhone Poulenc Sa | Nouvel antibiotique et sa préparation |
| US4012284A (en) * | 1962-11-16 | 1977-03-15 | Societa' Farmaceutici Italia, S.p.A. | Process of preparation of antibiotic F.I. 1762 derivatives |
| US3183157A (en) * | 1963-02-01 | 1965-05-11 | Upjohn Co | Antibiotic nogalamycin and method of producing |
| US4197249A (en) * | 1977-08-15 | 1980-04-08 | American Cyanamid Company | 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| GB8923075D0 (en) * | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| DE69527125T2 (de) * | 1994-11-09 | 2003-02-27 | Hitachi, Ltd. | Verfahren und Vorrichtung zur DNS Analyse |
| US5858397A (en) * | 1995-10-11 | 1999-01-12 | University Of British Columbia | Liposomal formulations of mitoxantrone |
| EP2369007B1 (en) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| ATE273381T1 (de) * | 1997-02-12 | 2004-08-15 | Eugene Y Chan | Verfahren zur analyse von polymeren |
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| KR100386606B1 (ko) * | 2001-02-03 | 2003-06-02 | 엘지전자 주식회사 | Ｄｎａ 검출 방법 및 그 장치 |
| KR100459394B1 (ko) * | 2001-10-30 | 2004-12-03 | 엘지전자 주식회사 | 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법 |
| AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| CA2500129C (en) * | 2002-09-30 | 2011-03-29 | F. Hoffmann-La Roche Ag | Oligonucleotides for genotyping thymidylate synthase gene |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| EP1694869A2 (en) * | 2003-11-10 | 2006-08-30 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2002
- 2002-10-10 GB GBGB0223563.8A patent/GB0223563D0/en not_active Ceased
-
2003
- 2003-10-10 CN CNA2003801056004A patent/CN1723288A/zh active Pending
- 2003-10-10 DE DE60327345T patent/DE60327345D1/de not_active Expired - Lifetime
- 2003-10-10 CA CA002501636A patent/CA2501636A1/en not_active Abandoned
- 2003-10-10 EP EP03758305A patent/EP1554404B1/en not_active Expired - Lifetime
- 2003-10-10 JP JP2004542659A patent/JP2006501836A/ja active Pending
- 2003-10-10 WO PCT/GB2003/004412 patent/WO2004033726A1/en not_active Ceased
- 2003-10-10 AT AT03758305T patent/ATE429513T1/de not_active IP Right Cessation
- 2003-10-10 AU AU2003274316A patent/AU2003274316A1/en not_active Abandoned
- 2003-10-10 US US10/530,980 patent/US20060127906A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0870876A (ja) * | 1994-09-01 | 1996-03-19 | F Hoffmann La Roche Ag | 発光性標識で標識されたオリゴヌクレオチドの分解検出方法 |
| WO2001092218A2 (en) * | 2000-05-31 | 2001-12-06 | Oridigm Corporation | Polyamine analogues as therapeutic and diagnostic agents |
| JP2004532044A (ja) * | 2001-05-25 | 2004-10-21 | イギリス国 | 検出システム |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009022512A1 (ja) * | 2007-08-14 | 2009-02-19 | Sony Corporation | 二本鎖核酸の形成に係る情報を得る方法 |
| JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1554404A1 (en) | 2005-07-20 |
| ATE429513T1 (de) | 2009-05-15 |
| CA2501636A1 (en) | 2004-04-22 |
| CN1723288A (zh) | 2006-01-18 |
| US20060127906A1 (en) | 2006-06-15 |
| GB0223563D0 (en) | 2002-11-20 |
| AU2003274316A1 (en) | 2004-05-04 |
| EP1554404B1 (en) | 2009-04-22 |
| WO2004033726A1 (en) | 2004-04-22 |
| DE60327345D1 (de) | 2009-06-04 |
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