WO2009022512A1 - 二本鎖核酸の形成に係る情報を得る方法 - Google Patents

二本鎖核酸の形成に係る情報を得る方法 Download PDF

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Publication number
WO2009022512A1
WO2009022512A1 PCT/JP2008/062706 JP2008062706W WO2009022512A1 WO 2009022512 A1 WO2009022512 A1 WO 2009022512A1 JP 2008062706 W JP2008062706 W JP 2008062706W WO 2009022512 A1 WO2009022512 A1 WO 2009022512A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
double
stranded nucleic
formation
obtaining information
Prior art date
Application number
PCT/JP2008/062706
Other languages
English (en)
French (fr)
Inventor
Tomoteru Abe
Original Assignee
Sony Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sony Corporation filed Critical Sony Corporation
Priority to US12/672,025 priority Critical patent/US20110059541A1/en
Priority to EP08791131A priority patent/EP2177612A4/en
Priority to CN200880102143.6A priority patent/CN101772573A/zh
Publication of WO2009022512A1 publication Critical patent/WO2009022512A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　蛍光を検出することによって二本鎖核酸の形成に係る情報を得る方法であって、簡便で様々な反応系に適用することができ、かつ、バックグラウンドシグナルを減少させた高検出感度の方法を提供する。二本鎖核酸の形成に係る情報を得る方法であって、一の核酸鎖に標識された標識蛍光色素と、二本鎖核酸の塩基対間に結合又は挿入して、前記蛍光色素との間でエネルギー移動をし得るインターカレーターとからなる検出子の蛍光に係る情報を検出することを特徴とする方法。
PCT/JP2008/062706 2007-08-14 2008-07-14 二本鎖核酸の形成に係る情報を得る方法 WO2009022512A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/672,025 US20110059541A1 (en) 2007-08-14 2008-07-14 Method for Obtaining Information on Formation of Double-Stranded Nucleic Acid
EP08791131A EP2177612A4 (en) 2007-08-14 2008-07-14 METHOD FOR OBTAINING INFORMATION FOR THE FORMATION OF DOUBLE-STRUCTURED NUCLEIC ACIDS
CN200880102143.6A CN101772573A (zh) 2007-08-14 2008-07-14 获得双链核酸形成信息的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007211366A JP2009044967A (ja) 2007-08-14 2007-08-14 二本鎖核酸の形成に係る情報を得る方法
JP2007-211366 2007-08-14

Publications (1)

Publication Number Publication Date
WO2009022512A1 true WO2009022512A1 (ja) 2009-02-19

Family

ID=40350567

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/062706 WO2009022512A1 (ja) 2007-08-14 2008-07-14 二本鎖核酸の形成に係る情報を得る方法

Country Status (5)

Country Link
US (1) US20110059541A1 (ja)
EP (1) EP2177612A4 (ja)
JP (1) JP2009044967A (ja)
CN (1) CN101772573A (ja)
WO (1) WO2009022512A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019528065A (ja) * 2016-08-19 2019-10-10 クアンタポール， インコーポレイテッド 消光剤を使用する光学に基づくナノポア配列決定
TWI617667B (zh) * 2017-03-03 2018-03-11 長庚大學 核酸分子檢測方法及檢測套組

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11290098A (ja) 1998-04-10 1999-10-26 Unitika Ltd 核酸染色剤、それを用いた二本鎖核酸の検出方法及び標的核酸の検出試薬
JP2001245699A (ja) 2001-02-01 2001-09-11 Hitachi Ltd 核酸の検出方法
JP2003274925A (ja) * 2002-03-26 2003-09-30 Juki Corp 自動核酸調製装置及びこれを用いたサンプル中の核酸検出方法
JP2005528121A (ja) * 2002-05-31 2005-09-22 セクレタリー・デパートメント・オブ・アトミック・エナジー 核酸増幅による標的核酸配列の検出の改良方法
JP2005291703A (ja) 2002-03-18 2005-10-20 Sangaku Renkei Kiko Kyushu:Kk 蛍光性インターカレート基を含有する二本鎖核酸検出用蛍光試薬
JP2006501836A (ja) * 2002-10-10 2006-01-19 イギリス国 検出システム

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19923168A1 (de) * 1999-05-20 2000-11-23 Roche Diagnostics Gmbh Neue Fluoreszenzfarbstoffe und ihre Verwendung als Fluoreszenzmarker
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
AU2002366046A1 (en) * 2001-10-19 2003-06-10 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
JP2003262591A (ja) * 2002-03-08 2003-09-19 Japan Science & Technology Corp タンパク質又は核酸の定量の最適化方法及び蛍光測定による物質の定量方法
JP4235902B2 (ja) * 2002-09-02 2009-03-11 東洋紡績株式会社 塩基多型の同定方法
WO2005047470A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Intercalator fret donors or acceptors

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11290098A (ja) 1998-04-10 1999-10-26 Unitika Ltd 核酸染色剤、それを用いた二本鎖核酸の検出方法及び標的核酸の検出試薬
JP2001245699A (ja) 2001-02-01 2001-09-11 Hitachi Ltd 核酸の検出方法
JP2005291703A (ja) 2002-03-18 2005-10-20 Sangaku Renkei Kiko Kyushu:Kk 蛍光性インターカレート基を含有する二本鎖核酸検出用蛍光試薬
JP2003274925A (ja) * 2002-03-26 2003-09-30 Juki Corp 自動核酸調製装置及びこれを用いたサンプル中の核酸検出方法
JP2005528121A (ja) * 2002-05-31 2005-09-22 セクレタリー・デパートメント・オブ・アトミック・エナジー 核酸増幅による標的核酸配列の検出の改良方法
JP2006501836A (ja) * 2002-10-10 2006-01-19 イギリス国 検出システム

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2177612A4

Also Published As

Publication number Publication date
EP2177612A4 (en) 2010-10-13
US20110059541A1 (en) 2011-03-10
CN101772573A (zh) 2010-07-07
JP2009044967A (ja) 2009-03-05
EP2177612A1 (en) 2010-04-21

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