JP2005538725A5 - - Google Patents

Info

Publication number

JP2005538725A5

JP2005538725A5 JP2004536347A JP2004536347A JP2005538725A5 JP 2005538725 A5 JP2005538725 A5 JP 2005538725A5 JP 2004536347 A JP2004536347 A JP 2004536347A JP 2004536347 A JP2004536347 A JP 2004536347A JP 2005538725 A5 JP2005538725 A5 JP 2005538725A5

Authority

JP

Japan

Prior art keywords

sequence

oligonucleotide

oligonucleotides

composition

primer

Prior art date

2003-09-12

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 108091034117 Oligonucleotide Proteins 0.000 claims description 71
• 238000000034 method Methods 0.000 claims description 37
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 30
• 238000002493 microarray Methods 0.000 claims description 15
• 239000007787 solid Substances 0.000 claims description 12
• 239000002773 nucleotide Substances 0.000 claims description 10
• 238000011065 in-situ storage Methods 0.000 claims description 9
• 125000003729 nucleotide group Chemical group 0.000 claims description 9
• 230000015572 biosynthetic process Effects 0.000 claims description 5
• 238000003786 synthesis reaction Methods 0.000 claims description 5
• 239000000126 substance Substances 0.000 claims description 4
• 239000000203 mixture Substances 0.000 claims 21
• 108091033319 polynucleotide Proteins 0.000 claims 15
• 239000002157 polynucleotide Substances 0.000 claims 15
• 102000040430 polynucleotide Human genes 0.000 claims 15
• 108091008146 restriction endonucleases Proteins 0.000 claims 13
• 230000000295 complement effect Effects 0.000 claims 11
• 125000005647 linker group Chemical group 0.000 claims 7
• 239000011324 bead Substances 0.000 claims 5
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
• 108010042407 Endonucleases Proteins 0.000 claims 4
• 102000004533 Endonucleases Human genes 0.000 claims 4
• 239000004065 semiconductor Substances 0.000 claims 4
• 238000000137 annealing Methods 0.000 claims 3
• 108090001008 Avidin Proteins 0.000 claims 2
• 108010090804 Streptavidin Proteins 0.000 claims 2
• XNBZPOHDTUWNMW-OUUCXATCSA-N alpha-L-Fucp-(1->2)-[alpha-D-Galp-(1->3)]-D-Galp Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@@H](CO)OC1O XNBZPOHDTUWNMW-OUUCXATCSA-N 0.000 claims 2
• 230000003321 amplification Effects 0.000 claims 2
• 229960002685 biotin Drugs 0.000 claims 2
• 235000020958 biotin Nutrition 0.000 claims 2
• 239000011616 biotin Substances 0.000 claims 2
• 238000006243 chemical reaction Methods 0.000 claims 2
• 238000003776 cleavage reaction Methods 0.000 claims 2
• 230000008878 coupling Effects 0.000 claims 2
• 238000010168 coupling process Methods 0.000 claims 2
• 238000005859 coupling reaction Methods 0.000 claims 2
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
• 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 2
• 238000002844 melting Methods 0.000 claims 2
• 230000008018 melting Effects 0.000 claims 2
• 238000003199 nucleic acid amplification method Methods 0.000 claims 2
• 230000007017 scission Effects 0.000 claims 2
• 150000003384 small molecules Chemical class 0.000 claims 2
• KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical group OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims 2
• 230000002194 synthesizing effect Effects 0.000 claims 2
• 238000012408 PCR amplification Methods 0.000 claims 1
• 238000011437 continuous method Methods 0.000 claims 1
• 238000002372 labelling Methods 0.000 claims 1
• 239000011521 glass Substances 0.000 description 4
• 238000000018 DNA microarray Methods 0.000 description 1
• XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
• 238000003491 array Methods 0.000 description 1
• 230000005684 electric field Effects 0.000 description 1
• 230000008030 elimination Effects 0.000 description 1
• 238000003379 elimination reaction Methods 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 239000003365 glass fiber Substances 0.000 description 1
• 238000007641 inkjet printing Methods 0.000 description 1
• 238000002515 oligonucleotide synthesis Methods 0.000 description 1
• 108090000623 proteins and genes Proteins 0.000 description 1
• 229910052710 silicon Inorganic materials 0.000 description 1
• 239000010703 silicon Substances 0.000 description 1