ATE143696T1 - Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung - Google Patents

Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung

Info

Publication number
ATE143696T1
ATE143696T1 AT90103830T AT90103830T ATE143696T1 AT E143696 T1 ATE143696 T1 AT E143696T1 AT 90103830 T AT90103830 T AT 90103830T AT 90103830 T AT90103830 T AT 90103830T AT E143696 T1 ATE143696 T1 AT E143696T1
Authority
AT
Austria
Prior art keywords
forming
oligonucleotides
double stranded
pair
stranded oligonucleotide
Prior art date
Application number
AT90103830T
Other languages
English (en)
Inventor
Nobuko Yamamoto
Kinya Kato
Harumi Iwashita
Masanori Sakuranaga
Original Assignee
Canon Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP26695689A external-priority patent/JPH03127999A/ja
Priority claimed from JP34313489A external-priority patent/JPH037583A/ja
Priority claimed from JP02003338A external-priority patent/JP3122106B2/ja
Priority claimed from JP2765390A external-priority patent/JPH03232489A/ja
Priority claimed from JP2930090A external-priority patent/JPH03232490A/ja
Application filed by Canon Kk filed Critical Canon Kk
Application granted granted Critical
Publication of ATE143696T1 publication Critical patent/ATE143696T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
AT90103830T 1989-02-28 1990-02-27 Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung ATE143696T1 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP4890889 1989-02-28
JP5073889 1989-03-01
JP26695689A JPH03127999A (ja) 1989-10-12 1989-10-12 オリゴヌクレオチドの形成方法
JP34313489A JPH037583A (ja) 1989-02-28 1989-12-29 部分的二本鎖オリゴヌクレオチド及びオリゴヌクレオチドの形成方法
JP02003338A JP3122106B2 (ja) 1990-01-12 1990-01-12 標的核酸の検出方法及びプローブの製造方法
JP2765390A JPH03232489A (ja) 1990-02-06 1990-02-06 部分的二本鎖オリゴヌクレオチド及びオリゴヌクレオチドの形成方法
JP2930090A JPH03232490A (ja) 1990-02-07 1990-02-07 オリゴヌクレオチドの形成方法

Publications (1)

Publication Number Publication Date
ATE143696T1 true ATE143696T1 (de) 1996-10-15

Family

ID=27563223

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90103830T ATE143696T1 (de) 1989-02-28 1990-02-27 Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung

Country Status (4)

Country Link
US (1) US5830643A (de)
EP (1) EP0385410B1 (de)
AT (1) ATE143696T1 (de)
DE (1) DE69028725T2 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4681693A (en) * 1992-07-17 1994-02-14 Aprogenex, Inc. Nucleic acid probes and uses thereof to detect double-stranded nucleic acids
WO1994012632A1 (en) * 1992-11-27 1994-06-09 University College London Improvements in nucleic acid synthesis by pcr
US6372424B1 (en) * 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US6673616B1 (en) * 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
IL120337A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
IL120338A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly
DE19736591A1 (de) * 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
AU9393398A (en) * 1997-09-16 1999-04-05 Egea Biosciences, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US7737088B1 (en) 1998-08-28 2010-06-15 Febit Holding Gmbh Method and device for producing biochemical reaction supporting materials
DE50013228D1 (de) * 1999-02-19 2006-09-07 Febit Biotech Gmbh Verfahren zur herstellung von polymeren
DE60227361D1 (de) 2001-01-19 2008-08-14 Centocor Inc Computer vermitteltes assembly von polynucleotiden kodierend für ein zielgerichtetes polypeptide
DE10164197A1 (de) * 2001-04-12 2003-07-17 Vladimir M Mirsky Steuerbare Verstärkung der Empfindlichkeit beim Hybridisierungsnachweis
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
JP4458327B2 (ja) * 2003-08-28 2010-04-28 キヤノン株式会社 標的物質の定量方法および該方法に用いるプローブ担体
JP2007508825A (ja) * 2003-10-15 2007-04-12 ブルー ヘロン バイオテクノロジー インコーポレイテッド ポリヌクレオチド生成のための固相方法
JP2006094760A (ja) * 2004-09-29 2006-04-13 Canon Inc 細胞のスクリーニング方法および口腔癌細胞検出方法
US8535914B2 (en) * 2005-01-21 2013-09-17 Canon Kabushiki Kaisha Probe, probe set and information acquisition method using the same
US20090011413A1 (en) * 2005-12-14 2009-01-08 Canon Kabushiki Kaisha Method for screening colon cancer cells and gene set used for examination of colon cancer
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
DK2398915T3 (en) 2009-02-20 2016-12-12 Synthetic Genomics Inc Synthesis of nucleic acids sequence verified
JP5729904B2 (ja) 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
EP2470653A1 (de) * 2009-08-26 2012-07-04 Research Development Foundation Verfahren zur erstellung von antikörperbibliotheken
CN103328652B (zh) * 2010-04-16 2016-12-28 宙斯科学有限公司 测量用于测定未纯化的样品中细胞生活力的酶活性的方法
KR102291045B1 (ko) 2013-08-05 2021-08-19 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
CN113604546A (zh) 2015-09-22 2021-11-05 特韦斯特生物科学公司 用于核酸合成的柔性基底
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
KR102217487B1 (ko) 2016-09-21 2021-02-23 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
EA201991262A1 (ru) 2016-12-16 2020-04-07 Твист Байосайенс Корпорейшн Библиотеки вариантов иммунологического синапса и их синтез
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
KR20240013290A (ko) 2017-06-12 2024-01-30 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
GB2581620A (en) 2017-09-11 2020-08-26 Twist Bioscience Corp GPCR binding proteins and synthesis thereof
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
EP3735459A4 (de) 2018-01-04 2021-10-06 Twist Bioscience Corporation Digitale informationsspeicherung auf dna-basis
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
CN114729342A (zh) 2019-06-21 2022-07-08 特韦斯特生物科学公司 基于条形码的核酸序列装配
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4605735A (en) * 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
SE8303626D0 (sv) * 1983-06-23 1983-06-23 Kabigen Ab A recombinant plasmid a transformant microorganism, a polydoxyrebonucleotide segment, a process for producing a biologically active protein, and the protein thus produced
DE3583361D1 (de) * 1984-06-14 1991-08-08 Ucp Gen Pharma Ag Verfahren zur herstellung von thrombin-inhibitoren.
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
EP0316018A3 (de) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
EP0196762A1 (de) * 1985-03-29 1986-10-08 Cetus Corporation Rekombinante Ricinfragmente, diese exprimierende Vektoren und transformierte Wirte, die Modifikation von DNS-Sequenzen und Isolierung von mRNS
US4767699A (en) * 1985-05-02 1988-08-30 Allied Corporation Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection
US4797355A (en) * 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
US4752566A (en) * 1985-12-17 1988-06-21 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
CA1317535C (en) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Assay of sequences using amplified genes
US4963602A (en) * 1989-11-13 1990-10-16 Hi-Tek Polymers, Inc. Aqueous epoxy resin-acrylic resin coating compositions containing also phenoxy, novolac and resole resin combination

Also Published As

Publication number Publication date
DE69028725D1 (de) 1996-11-07
EP0385410A3 (de) 1991-07-17
DE69028725T2 (de) 1997-03-13
US5830643A (en) 1998-11-03
EP0385410A2 (de) 1990-09-05
EP0385410B1 (de) 1996-10-02

Similar Documents

Publication Publication Date Title
ATE143696T1 (de) Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung
DE69522222D1 (de) Verarbeitbare, elektrisch leitfähige polyamilinzusammensetzungen und verfahren zu ihrer herstellung
EP1108783A3 (de) Oligonukletiden-gesteuerte Nukleinsäurerekombination
DE69022085D1 (de) Isolierter elektrischer Draht und Verfahren zu dessen Herstellung.
DE3863866D1 (de) Verfahren zur herstellung von 1-olefinpolymeren.
DE69801489D1 (de) Verfahren zur herstellung von 1,1,1,3,3-pentafluorpropan
ATE428780T1 (de) Verfahren zur zubereitung von antisense oligonukleotiden
DE69527611T2 (de) Verfahren zur herstellung von kupfer-pyrithion
DE69006252T2 (de) Elektrischer Steckerstift und Verfahren zu seiner Herstellung.
WO1999016780A3 (en) Genetic sequences, diagnostic and/or quantification methods for the identification of staphylococci strains
ATE262377T1 (de) Katalysatoren zur oxychlorierung von ethylen, verfahren zu ihrer herstellung, und oxychlorierungsverfahren
DE69731112D1 (de) Verfahren zur synthese von phosphorothioat-oligonukelotiden
CA2042808A1 (en) Method of Extracting Particular Nucleic Acid Fragment
EP0812911A3 (de) Methode zur Bildung makromolekularer Mikrogenpolymeren
DE3879843T2 (de) Verfahren zur herstellung von 3,5,5-trimethylcyclohexa-2-en-1,4-dion.
DE69514122T2 (de) Lösungsmittelfreies verfahren zur herstellung von 2,6 difluorbenzonitril
DE3877324T2 (de) Verfahren zur herstellung von acrylamidomethansulfonsaeure-polymeren.
DE975799T1 (de) Verfahren zur identifizierung von nukleinsäure-sequenzen, die im zusammenhang mit apomixie stehen
DE3580726D1 (de) Dynamoelektrisches flachspulbauteil, dessen ausgangsform, verfahren und geraet zur herstellung.
DE69923163D1 (de) Verfahren zur herstellung von 1,2,4-butantriol
DE3673508D1 (de) Verfahren zur herstellung von umformgesenken aus graphit.
DE3877461D1 (de) Verfahren zur herstellung von phenylenaetheramid-pfropfpolymeren.
DE3862616D1 (de) Verfahren zur herstellung von monomeren alkenylphenolen aus dihydroxydiphenylalkanen.
EP0871772A4 (de) Reste, die die bindung eines dritten stranges an komplementaere nukleinsaeure-duplexe vermitteln, gleich welcher basenpaarsequenz
ATE208368T1 (de) Verfahren zur herstellung von 3,4 disubstituirten dinitroanilinen