DE69731112D1 - Verfahren zur synthese von phosphorothioat-oligonukelotiden - Google Patents

Verfahren zur synthese von phosphorothioat-oligonukelotiden

Info

Publication number
DE69731112D1
DE69731112D1 DE69731112T DE69731112T DE69731112D1 DE 69731112 D1 DE69731112 D1 DE 69731112D1 DE 69731112 T DE69731112 T DE 69731112T DE 69731112 T DE69731112 T DE 69731112T DE 69731112 D1 DE69731112 D1 DE 69731112D1
Authority
DE
Germany
Prior art keywords
oligonucelotides
synthesising
phosphorothioate
methods
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69731112T
Other languages
English (en)
Other versions
DE69731112T2 (de
Inventor
L Cole
Vasulinga Ravikumar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ionis Pharmaceuticals Inc
Original Assignee
Isis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc
Application granted granted Critical
Publication of DE69731112D1 publication Critical patent/DE69731112D1/de
Publication of DE69731112T2 publication Critical patent/DE69731112T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69731112T 1996-12-27 1997-12-12 Verfahren zur synthese von phosphorothioat-oligonukelotiden Expired - Fee Related DE69731112T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/775,011 US6172217B1 (en) 1996-12-27 1996-12-27 Method of synthesizing phosphorothioate oligonucleotides
US775011 1996-12-27
PCT/US1997/023601 WO1998029427A1 (en) 1996-12-27 1997-12-12 Method of synthesizing phosphorothioate oligonucleotides

Publications (2)

Publication Number Publication Date
DE69731112D1 true DE69731112D1 (de) 2004-11-11
DE69731112T2 DE69731112T2 (de) 2006-02-23

Family

ID=25103044

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69731112T Expired - Fee Related DE69731112T2 (de) 1996-12-27 1997-12-12 Verfahren zur synthese von phosphorothioat-oligonukelotiden

Country Status (9)

Country Link
US (4) US6172217B1 (de)
EP (1) EP0948512B1 (de)
JP (1) JP2000511933A (de)
KR (1) KR100458979B1 (de)
AT (1) ATE278702T1 (de)
AU (1) AU721750B2 (de)
CA (1) CA2276213C (de)
DE (1) DE69731112T2 (de)
WO (1) WO1998029427A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6172217B1 (en) * 1996-12-27 2001-01-09 Isis Pharmaceuticals Inc. Method of synthesizing phosphorothioate oligonucleotides
FR2803848B1 (fr) * 2000-01-19 2002-02-15 Adir Nouveaux derives de benzenesulfonamide, leur procede de preparation et les compositions pharmaceutiques qui les contiennent
DE10041539A1 (de) * 2000-08-24 2002-03-07 Febit Ferrarius Biotech Gmbh Neue Amiditderivate zur Synthese von Polymeren auf Oberflächen
US20040198640A1 (en) * 2003-04-02 2004-10-07 Dharmacon, Inc. Stabilized polynucleotides for use in RNA interference
KR101147147B1 (ko) 2004-04-01 2012-05-25 머크 샤프 앤드 돔 코포레이션 Ｒｎａ 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
US7923206B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Method of determining a cellular response to a biological agent
US7935811B2 (en) 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US7923207B2 (en) 2004-11-22 2011-04-12 Dharmacon, Inc. Apparatus and system having dry gene silencing pools
EP2064223B1 (de) 2006-09-22 2013-04-24 Dharmacon, Inc. Duplexoligonukleotide und Verfahren zum Gen-Silencing durch RNA-Interferenz
KR100886139B1 (ko) * 2007-11-13 2009-02-27 주식회사 삼천리제약 올리고뉴클레오타이드의 제조방법
US8188060B2 (en) 2008-02-11 2012-05-29 Dharmacon, Inc. Duplex oligonucleotides with enhanced functionality in gene regulation

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US5132418A (en) 1980-02-29 1992-07-21 University Patents, Inc. Process for preparing polynucleotides
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4500707A (en) 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4668777A (en) 1981-03-27 1987-05-26 University Patents, Inc. Phosphoramidite nucleoside compounds
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
USRE34069E (en) 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
GB8607265D0 (en) * 1986-03-24 1986-04-30 London King S College Protecting groups of organic synthesis
DE68927417T2 (de) 1988-04-27 1997-03-20 Isis Pharmaceutical Inc Oligoribonukleotid-Derivate und ihre Anwendung als antivirale Mittel
US5449769A (en) 1989-03-06 1995-09-12 Gen-Probe Incorporated Method and reagent for sulfurization of organophosphorous compounds
ATE269870T1 (de) 1989-10-24 2004-07-15 Isis Pharmaceuticals Inc 2'-modifizierte oligonukleotide
US5506212A (en) 1990-01-11 1996-04-09 Isis Pharmaceuticals, Inc. Oligonucleotides with substantially chirally pure phosphorothioate linkages
US5212295A (en) 1990-01-11 1993-05-18 Isis Pharmaceuticals Monomers for preparation of oligonucleotides having chiral phosphorus linkages
WO1991010671A1 (en) 1990-01-11 1991-07-25 Isis Pharmaceuticals, Inc. Compositions and methods for detecting and modulating rna activity and gene expression
US5635488A (en) 1991-10-15 1997-06-03 Isis Pharmaceuticals, Inc. Compounds having phosphorodithioate linkages of high chiral purity
US5166387A (en) 1990-01-12 1992-11-24 Applied Biosystems, Inc. Method of synthesizing sulfurized oligonucleotide analogs with thiuram disulfides
US5292875A (en) 1990-04-20 1994-03-08 Lynx Therapeutics, Inc. Method of synthesizing sulfurized oligonucleotide analogs
US5151510A (en) 1990-04-20 1992-09-29 Applied Biosystems, Inc. Method of synethesizing sulfurized oligonucleotide analogs
US5672697A (en) * 1991-02-08 1997-09-30 Gilead Sciences, Inc. Nucleoside 5'-methylene phosphonates
US5512668A (en) 1991-03-06 1996-04-30 Polish Academy Of Sciences Solid phase oligonucleotide synthesis using phospholane intermediates
US5525719A (en) * 1991-08-30 1996-06-11 Chemgenes Corporation N-protected-2'-O-methyl-and N-protected-3'-O-methyl-ribonucleosides and their phosphoramidite derivatives
GB9127243D0 (en) 1991-12-23 1992-02-19 King S College London Synthesis of phosphorothioate analogues of oligonucleotides
CA2153505A1 (en) 1993-01-08 1994-07-21 Jin-Yan Tang Synthesis of dimmer blocks and their use in assembling oligonucleotides
US6015886A (en) * 1993-05-24 2000-01-18 Chemgenes Corporation Oligonucleotide phosphate esters
US5571902A (en) 1993-07-29 1996-11-05 Isis Pharmaceuticals, Inc. Synthesis of oligonucleotides
JPH07157498A (ja) 1993-12-07 1995-06-20 Sankyo Co Ltd 短鎖ホスホロチオエート結合オリゴデオキシヌクレオチド
WO1995026204A1 (en) 1994-03-25 1995-10-05 Isis Pharmaceuticals, Inc. Immune stimulation by phosphorothioate oligonucleotide analogs
US5405985A (en) 1994-07-08 1995-04-11 The Goodyear Tire & Rubber Company Preparation of sulfur-containing organosilicon compounds
DE4438918A1 (de) * 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
CA2216844A1 (en) 1995-03-31 1996-10-03 Drug Delivery System Institute, Ltd. Amidite derivatives and oligonucleotide derivatives
US6172217B1 (en) * 1996-12-27 2001-01-09 Isis Pharmaceuticals Inc. Method of synthesizing phosphorothioate oligonucleotides
US6649750B1 (en) * 2000-01-05 2003-11-18 Isis Pharmaceuticals, Inc. Process for the preparation of oligonucleotide compounds

Also Published As

Publication number Publication date
DE69731112T2 (de) 2006-02-23
US20030114662A1 (en) 2003-06-19
JP2000511933A (ja) 2000-09-12
US20020128466A1 (en) 2002-09-12
US20010003132A1 (en) 2001-06-07
AU5900998A (en) 1998-07-31
KR100458979B1 (ko) 2004-12-03
EP0948512A1 (de) 1999-10-13
EP0948512B1 (de) 2004-10-06
US6403781B2 (en) 2002-06-11
WO1998029427A1 (en) 1998-07-09
US6172217B1 (en) 2001-01-09
CA2276213C (en) 2003-12-02
KR20000069706A (ko) 2000-11-25
EP0948512A4 (de) 2001-10-24
CA2276213A1 (en) 1998-07-09
AU721750B2 (en) 2000-07-13
ATE278702T1 (de) 2004-10-15
US6780989B2 (en) 2004-08-24

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee