JP2005518217A5 - - Google Patents
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- Publication number
- JP2005518217A5 JP2005518217A5 JP2003571490A JP2003571490A JP2005518217A5 JP 2005518217 A5 JP2005518217 A5 JP 2005518217A5 JP 2003571490 A JP2003571490 A JP 2003571490A JP 2003571490 A JP2003571490 A JP 2003571490A JP 2005518217 A5 JP2005518217 A5 JP 2005518217A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- amplification
- region
- primer
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 39
- 150000007523 nucleic acids Chemical class 0.000 claims 28
- 108020004707 nucleic acids Proteins 0.000 claims 27
- 102000039446 nucleic acids Human genes 0.000 claims 27
- 230000003321 amplification Effects 0.000 claims 24
- 238000003199 nucleic acid amplification method Methods 0.000 claims 24
- 241000894007 species Species 0.000 claims 15
- 238000003556 assay Methods 0.000 claims 8
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 8
- 108020004414 DNA Proteins 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 4
- 230000004048 modification Effects 0.000 claims 4
- 238000012986 modification Methods 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 238000000137 annealing Methods 0.000 claims 3
- 238000007385 chemical modification Methods 0.000 claims 3
- 201000010099 disease Diseases 0.000 claims 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 3
- 230000001105 regulatory effect Effects 0.000 claims 3
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 2
- 108010070675 Glutathione transferase Proteins 0.000 claims 2
- 102100029100 Hematopoietic prostaglandin D synthase Human genes 0.000 claims 2
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 2
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 230000002159 abnormal effect Effects 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 229960003237 betaine Drugs 0.000 claims 2
- 201000011510 cancer Diseases 0.000 claims 2
- 229940104302 cytosine Drugs 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- 230000011987 methylation Effects 0.000 claims 2
- 238000007069 methylation reaction Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 229940035893 uracil Drugs 0.000 claims 2
- 108020004465 16S ribosomal RNA Proteins 0.000 claims 1
- 229930024421 Adenine Natural products 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 206010006187 Breast cancer Diseases 0.000 claims 1
- 208000026310 Breast neoplasm Diseases 0.000 claims 1
- 108091029430 CpG site Proteins 0.000 claims 1
- 230000004544 DNA amplification Effects 0.000 claims 1
- 230000030933 DNA methylation on cytosine Effects 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000009004 PCR Kit Methods 0.000 claims 1
- 206010060862 Prostate cancer Diseases 0.000 claims 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims 1
- 101001032469 Xenopus laevis Glutathione S-transferase P 1 Proteins 0.000 claims 1
- 230000001594 aberrant effect Effects 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- 108091036078 conserved sequence Proteins 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 101150008380 gstp1 gene Proteins 0.000 claims 1
- 201000007270 liver cancer Diseases 0.000 claims 1
- 208000014018 liver neoplasm Diseases 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 101150082998 pi gene Proteins 0.000 claims 1
- 239000000376 reactant Substances 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 108020004418 ribosomal RNA Proteins 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPS0768A AUPS076802A0 (en) | 2002-02-26 | 2002-02-26 | Selective nucleic acid amplification |
| PCT/AU2003/000244 WO2003072810A1 (en) | 2002-02-26 | 2003-02-26 | Headloop dna amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005518217A JP2005518217A (ja) | 2005-06-23 |
| JP2005518217A5 true JP2005518217A5 (https=) | 2005-12-22 |
| JP4417723B2 JP4417723B2 (ja) | 2010-02-17 |
Family
ID=3834367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003571490A Expired - Fee Related JP4417723B2 (ja) | 2002-02-26 | 2003-02-26 | ヘッドループｄｎａの増幅 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7618773B2 (https=) |
| EP (1) | EP1483406A4 (https=) |
| JP (1) | JP4417723B2 (https=) |
| CN (1) | CN100516234C (https=) |
| AU (1) | AUPS076802A0 (https=) |
| CA (1) | CA2477573A1 (https=) |
| WO (1) | WO2003072810A1 (https=) |
| ZA (1) | ZA200407147B (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE554187T1 (de) * | 2007-03-08 | 2012-05-15 | Idaho Technology | Verfahren für die schmelzanalyse der nukleinsäuren |
| EP2167964B1 (de) * | 2007-06-13 | 2015-03-11 | Attomol GmbH Molekulare Diagnostika | Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat |
| EP2071035A1 (en) | 2007-12-13 | 2009-06-17 | Epigenomics AG | Facilitator and method for amplification |
| AU2011224534B2 (en) | 2010-03-08 | 2014-10-09 | Dana-Farber Cancer Institute, Inc. | Full COLD-PCR enrichment with reference blocking sequence |
| EP2691541B1 (en) | 2011-03-31 | 2017-10-18 | Dana-Farber Cancer Institute, Inc. | Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences |
| US20140106354A1 (en) | 2011-04-18 | 2014-04-17 | Garvan Institute Of Medical Research | Method of Diagnosing Cancer |
| WO2012162621A1 (en) | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
| US20140322717A1 (en) * | 2011-11-18 | 2014-10-30 | The Trustees Of Columbia University In The City Of New York | Selective amplification and detection of mutant gene alleles |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| EP3194624B1 (en) | 2014-09-15 | 2022-02-16 | Garvan Institute of Medical Research | Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| WO2018111835A1 (en) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| WO2018209092A1 (en) | 2017-05-10 | 2018-11-15 | Board Of Regents, The University Of Texas System | Methods and devices related to amplifying nucleic acid at a variety of temperatures |
| US11174511B2 (en) | 2017-07-24 | 2021-11-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture |
| US10513730B2 (en) * | 2017-11-22 | 2019-12-24 | Safeguard Biosystems Holdings Ltd. | Asymmetric PCR methods, primers and kits |
| EP3599283A1 (en) * | 2018-07-25 | 2020-01-29 | Blue DNA Companion | Method for assessing fecal pollution in water |
| CN110283913A (zh) * | 2019-07-21 | 2019-09-27 | 广州奥百阕谱生物科技有限公司 | Rassf1a基因甲基化状态检测试剂盒及其应用 |
| CN110607369A (zh) * | 2019-09-24 | 2019-12-24 | 广州奥百阕谱生物科技有限公司 | Septin9基因甲基化状态检测试剂盒及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5565340A (en) * | 1995-01-27 | 1996-10-15 | Clontech Laboratories, Inc. | Method for suppressing DNA fragment amplification during PCR |
| AUPP312998A0 (en) * | 1998-04-23 | 1998-05-14 | Commonwealth Scientific And Industrial Research Organisation | Diagnostic assay |
| GB9812768D0 (en) * | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
-
2002
- 2002-02-26 AU AUPS0768A patent/AUPS076802A0/en not_active Abandoned
-
2003
- 2003-02-26 WO PCT/AU2003/000244 patent/WO2003072810A1/en not_active Ceased
- 2003-02-26 US US10/505,779 patent/US7618773B2/en not_active Expired - Fee Related
- 2003-02-26 CN CNB03809391XA patent/CN100516234C/zh not_active Expired - Fee Related
- 2003-02-26 EP EP03707898A patent/EP1483406A4/en not_active Withdrawn
- 2003-02-26 JP JP2003571490A patent/JP4417723B2/ja not_active Expired - Fee Related
- 2003-02-26 CA CA002477573A patent/CA2477573A1/en not_active Abandoned
-
2004
- 2004-09-07 ZA ZA200407147A patent/ZA200407147B/en unknown
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