CN100516234C - Headloop DNA扩增 - Google Patents

Headloop DNA扩增 Download PDF

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Publication number
CN100516234C
CN100516234C CNB03809391XA CN03809391A CN100516234C CN 100516234 C CN100516234 C CN 100516234C CN B03809391X A CNB03809391X A CN B03809391XA CN 03809391 A CN03809391 A CN 03809391A CN 100516234 C CN100516234 C CN 100516234C
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CN
China
Prior art keywords
primer
nucleic acid
region
amplification
dna
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Expired - Fee Related
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CNB03809391XA
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English (en)
Chinese (zh)
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CN1650029A (zh
Inventor
基思·兰德
彼得·劳伦斯·莫洛伊
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB03809391XA 2002-02-26 2003-02-26 Headloop DNA扩增 Expired - Fee Related CN100516234C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPS0768A AUPS076802A0 (en) 2002-02-26 2002-02-26 Selective nucleic acid amplification
AUPS0768 2002-02-26

Publications (2)

Publication Number Publication Date
CN1650029A CN1650029A (zh) 2005-08-03
CN100516234C true CN100516234C (zh) 2009-07-22

Family

ID=3834367

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB03809391XA Expired - Fee Related CN100516234C (zh) 2002-02-26 2003-02-26 Headloop DNA扩增

Country Status (8)

Country Link
US (1) US7618773B2 (https=)
EP (1) EP1483406A4 (https=)
JP (1) JP4417723B2 (https=)
CN (1) CN100516234C (https=)
AU (1) AUPS076802A0 (https=)
CA (1) CA2477573A1 (https=)
WO (1) WO2003072810A1 (https=)
ZA (1) ZA200407147B (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE554187T1 (de) * 2007-03-08 2012-05-15 Idaho Technology Verfahren für die schmelzanalyse der nukleinsäuren
EP2167964B1 (de) * 2007-06-13 2015-03-11 Attomol GmbH Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
EP2071035A1 (en) 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
US20140106354A1 (en) 2011-04-18 2014-04-17 Garvan Institute Of Medical Research Method of Diagnosing Cancer
WO2012162621A1 (en) 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
US20140322717A1 (en) * 2011-11-18 2014-10-30 The Trustees Of Columbia University In The City Of New York Selective amplification and detection of mutant gene alleles
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP3194624B1 (en) 2014-09-15 2022-02-16 Garvan Institute of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2018209092A1 (en) 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
US10513730B2 (en) * 2017-11-22 2019-12-24 Safeguard Biosystems Holdings Ltd. Asymmetric PCR methods, primers and kits
EP3599283A1 (en) * 2018-07-25 2020-01-29 Blue DNA Companion Method for assessing fecal pollution in water
CN110283913A (zh) * 2019-07-21 2019-09-27 广州奥百阕谱生物科技有限公司 Rassf1a基因甲基化状态检测试剂盒及其应用
CN110607369A (zh) * 2019-09-24 2019-12-24 广州奥百阕谱生物科技有限公司 Septin9基因甲基化状态检测试剂盒及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565340A (en) * 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
WO1999055905A1 (en) * 1998-04-23 1999-11-04 Commonwealth Scientific And Industrial Research Organisation ASSAY FOR METHYLATION IN THE GST-Pi GENE

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565340A (en) * 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
WO1999055905A1 (en) * 1998-04-23 1999-11-04 Commonwealth Scientific And Industrial Research Organisation ASSAY FOR METHYLATION IN THE GST-Pi GENE

Also Published As

Publication number Publication date
CA2477573A1 (en) 2003-09-04
WO2003072810A1 (en) 2003-09-04
US7618773B2 (en) 2009-11-17
AUPS076802A0 (en) 2002-03-21
JP2005518217A (ja) 2005-06-23
JP4417723B2 (ja) 2010-02-17
EP1483406A4 (en) 2007-10-24
EP1483406A1 (en) 2004-12-08
US20050221312A1 (en) 2005-10-06
CN1650029A (zh) 2005-08-03
ZA200407147B (en) 2006-02-22

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Granted publication date: 20090722

Termination date: 20140226