JP4417723B2 - ヘッドループｄｎａの増幅 - Google Patents

ヘッドループｄｎａの増幅 Download PDF

Info

Publication number
JP4417723B2
JP4417723B2 JP2003571490A JP2003571490A JP4417723B2 JP 4417723 B2 JP4417723 B2 JP 4417723B2 JP 2003571490 A JP2003571490 A JP 2003571490A JP 2003571490 A JP2003571490 A JP 2003571490A JP 4417723 B2 JP4417723 B2 JP 4417723B2
Authority
JP
Japan
Prior art keywords
amplification
primer
nucleic acid
region
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2003571490A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005518217A5 (https=
JP2005518217A (ja
Inventor
キース・ランド
ピーター・ローレンス・モロイ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of JP2005518217A publication Critical patent/JP2005518217A/ja
Publication of JP2005518217A5 publication Critical patent/JP2005518217A5/ja
Application granted granted Critical
Publication of JP4417723B2 publication Critical patent/JP4417723B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2003571490A 2002-02-26 2003-02-26 ヘッドループｄｎａの増幅 Expired - Fee Related JP4417723B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPS0768A AUPS076802A0 (en) 2002-02-26 2002-02-26 Selective nucleic acid amplification
PCT/AU2003/000244 WO2003072810A1 (en) 2002-02-26 2003-02-26 Headloop dna amplification

Publications (3)

Publication Number Publication Date
JP2005518217A JP2005518217A (ja) 2005-06-23
JP2005518217A5 JP2005518217A5 (https=) 2005-12-22
JP4417723B2 true JP4417723B2 (ja) 2010-02-17

Family

ID=3834367

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003571490A Expired - Fee Related JP4417723B2 (ja) 2002-02-26 2003-02-26 ヘッドループｄｎａの増幅

Country Status (8)

Country Link
US (1) US7618773B2 (https=)
EP (1) EP1483406A4 (https=)
JP (1) JP4417723B2 (https=)
CN (1) CN100516234C (https=)
AU (1) AUPS076802A0 (https=)
CA (1) CA2477573A1 (https=)
WO (1) WO2003072810A1 (https=)
ZA (1) ZA200407147B (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE554187T1 (de) * 2007-03-08 2012-05-15 Idaho Technology Verfahren für die schmelzanalyse der nukleinsäuren
EP2167964B1 (de) * 2007-06-13 2015-03-11 Attomol GmbH Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
EP2071035A1 (en) 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
US20140106354A1 (en) 2011-04-18 2014-04-17 Garvan Institute Of Medical Research Method of Diagnosing Cancer
WO2012162621A1 (en) 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
US20140322717A1 (en) * 2011-11-18 2014-10-30 The Trustees Of Columbia University In The City Of New York Selective amplification and detection of mutant gene alleles
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP3194624B1 (en) 2014-09-15 2022-02-16 Garvan Institute of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2018209092A1 (en) 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
US10513730B2 (en) * 2017-11-22 2019-12-24 Safeguard Biosystems Holdings Ltd. Asymmetric PCR methods, primers and kits
EP3599283A1 (en) * 2018-07-25 2020-01-29 Blue DNA Companion Method for assessing fecal pollution in water
CN110283913A (zh) * 2019-07-21 2019-09-27 广州奥百阕谱生物科技有限公司 Rassf1a基因甲基化状态检测试剂盒及其应用
CN110607369A (zh) * 2019-09-24 2019-12-24 广州奥百阕谱生物科技有限公司 Septin9基因甲基化状态检测试剂盒及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565340A (en) * 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
AUPP312998A0 (en) * 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods

Also Published As

Publication number Publication date
CA2477573A1 (en) 2003-09-04
WO2003072810A1 (en) 2003-09-04
US7618773B2 (en) 2009-11-17
AUPS076802A0 (en) 2002-03-21
JP2005518217A (ja) 2005-06-23
EP1483406A4 (en) 2007-10-24
EP1483406A1 (en) 2004-12-08
US20050221312A1 (en) 2005-10-06
CN1650029A (zh) 2005-08-03
CN100516234C (zh) 2009-07-22
ZA200407147B (en) 2006-02-22

Similar Documents

Publication Publication Date Title
JP4417723B2 (ja) ヘッドループｄｎａの増幅
CN106661627B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
US8431347B2 (en) Isothermal strand displacement amplification using primers containing a non-regular base
AU2018260633B2 (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
US8455197B2 (en) Nucleic acid amplification
US6063604A (en) Target nucleic acid sequence amplification
JP2012515528A (ja) 改良された等温鎖置換増幅
JP5336350B2 (ja) Ｄｎａフラグメントの増幅
JP2018514230A (ja) 限られたヌクレオチド組成を有するプライマーを用いた増幅
JP2005518216A (ja) 融解温度依存ｄｎａ増幅
JP2022024071A (ja) 標的核酸を検出する方法
JPWO2010026933A1 (ja) Ｒｎａ検出用組成物
WO2001081630A2 (en) Template-specific termination in a polymerase chain reaction
AU2003212087B2 (en) Headloop DNA amplification
JP7306722B2 (ja) プライマー及びその使用
JP5299964B2 (ja) Ｄｎａ３’末端の修飾基除去用酵素試薬
AU2003209812B2 (en) Melting temperature dependent DNA amplification
JP2009219445A (ja) Ｖｋｏｒｃ１遺伝子上の１塩基多型を検出する方法およびプライマーセット
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20060227

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20081202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090302

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090309

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090430

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090707

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20091002

TRDD Decision of grant or rejection written
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20091002

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20091027

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20091126

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121204

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121204

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20131204

Year of fee payment: 4

LAPS Cancellation because of no payment of annual fees