JP2005500054A5 - - Google Patents
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- Publication number
- JP2005500054A5 JP2005500054A5 JP2003519489A JP2003519489A JP2005500054A5 JP 2005500054 A5 JP2005500054 A5 JP 2005500054A5 JP 2003519489 A JP2003519489 A JP 2003519489A JP 2003519489 A JP2003519489 A JP 2003519489A JP 2005500054 A5 JP2005500054 A5 JP 2005500054A5
- Authority
- JP
- Japan
- Prior art keywords
- expression vector
- target protein
- cell surface
- gram
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims 20
- 102000004169 proteins and genes Human genes 0.000 claims 15
- 239000013604 expression vector Substances 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 11
- 244000005700 microbiome Species 0.000 claims 10
- 241000894006 Bacteria Species 0.000 claims 6
- 238000000034 method Methods 0.000 claims 4
- 101100059000 Bacillus subtilis (strain 168) capA gene Proteins 0.000 claims 3
- 101100059011 Bacillus subtilis (strain 168) capB gene Proteins 0.000 claims 3
- 101100219452 Bacillus subtilis (strain 168) capC gene Proteins 0.000 claims 3
- 101100083407 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) pgsA1 gene Proteins 0.000 claims 3
- 101150103033 lpxB gene Proteins 0.000 claims 3
- 101150076330 pgsA gene Proteins 0.000 claims 3
- 241000192125 Firmicutes Species 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 2
- 108090000364 Ligases Proteins 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20010048373 | 2001-08-10 | ||
| PCT/KR2002/001522 WO2003014360A1 (en) | 2001-08-10 | 2002-08-09 | SURFACE EXPRESSION VECTORS HAVING pgsBCA, THE GENE CODING POLY-GAMMA-GLUTAMATE SYNTHETASE, AND A METHOD FOR EXPRESSION OF TARGET PROTEIN AT THE SURFACE OF MICROORGANISM USING THE VECTOR |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005500054A JP2005500054A (ja) | 2005-01-06 |
| JP2005500054A5 true JP2005500054A5 (enExample) | 2006-01-12 |
| JP4458844B2 JP4458844B2 (ja) | 2010-04-28 |
Family
ID=19713096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003519489A Expired - Fee Related JP4458844B2 (ja) | 2001-08-10 | 2002-08-09 | ｐｇｓＢＣＡ、ポリ−γ−グルタミン酸シンテターゼをコードする遺伝子を有する表面発現用のベクター、及びこれを用いた標的タンパク質の微生物表面発現方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7553636B2 (enExample) |
| EP (1) | EP1421195B1 (enExample) |
| JP (1) | JP4458844B2 (enExample) |
| KR (1) | KR100469800B1 (enExample) |
| CN (1) | CN1311082C (enExample) |
| AT (1) | ATE342990T1 (enExample) |
| DE (1) | DE60215521T2 (enExample) |
| WO (1) | WO2003014360A1 (enExample) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100525759B1 (ko) * | 2002-10-17 | 2005-11-03 | 한국생명공학연구원 | 바실러스 속 균주 유래의 폴리 감마 글루탐산합성유전자를 이용한 펩타이드 항생물질 Ｐ5와Ａｎａｌ3의 표면 발현 방법 및 그의 용도 |
| AU2003271215A1 (en) | 2002-10-17 | 2004-05-04 | Bioleaders Corporation | Vector for anti-hpv vaccine and transformed microorganism by the vector |
| AU2004245859B2 (en) * | 2003-06-04 | 2007-02-08 | Bioleaders Corporation | Cell surface expression vector of SARS virus antigen and microorganisms transformed thereby |
| KR20050079162A (ko) * | 2004-02-04 | 2005-08-09 | 주식회사 바이오리더스 | 파보바이러스 항원의 세포표면 발현벡터 및 상기 벡터에의해 형질전환된 미생물 |
| US7255855B2 (en) * | 2004-02-27 | 2007-08-14 | Bioleaders Corporation | Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase |
| JP2005312426A (ja) * | 2004-03-31 | 2005-11-10 | Bioleaders Japan:Kk | タンパク質を表層提示する大腸菌 |
| AU2006242770A1 (en) * | 2005-03-04 | 2006-11-09 | Suntory Holdings Limited | Transgenic plant for producing polyglutamic acid |
| JP2006262724A (ja) * | 2005-03-22 | 2006-10-05 | Kobe Univ | タンパク質高発現乳酸菌 |
| WO2006126682A1 (ja) * | 2005-05-27 | 2006-11-30 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | アルツハイマー病の予防・治療用ワクチン |
| KR100872042B1 (ko) * | 2005-09-14 | 2008-12-05 | 주식회사 바이오리더스 | 마이오스타틴을 발현하는 세포표면 발현벡터 및 상기벡터에 의해 형질전환된 미생물 |
| KR100578395B1 (ko) * | 2005-11-11 | 2006-05-10 | 주식회사 바이오리더스 | 면역기능이 강화된 사균화 유산균 제제 및 그 제조방법 |
| KR100782332B1 (ko) * | 2006-01-23 | 2007-12-06 | 주식회사 바이오리더스 | 새우 흰 반점 바이러스 항원의 표면 발현벡터 및 이에 의해형질전환된 미생물 |
| KR100985914B1 (ko) * | 2006-12-13 | 2010-10-08 | 주식회사 바이오리더스 | 간암 특이 항원인 알파페토단백질의 표면발현벡터 및 이에의해 형질전환된 미생물 |
| KR100972843B1 (ko) * | 2007-03-22 | 2010-07-28 | 주식회사 바이오리더스 | 신규 항시적 고발현 프로모터 및 그 용도 |
| KR100857861B1 (ko) * | 2007-10-15 | 2008-09-11 | 주식회사 바이오리더스 | Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물 |
| KR101471043B1 (ko) | 2009-01-08 | 2014-12-09 | 주식회사 바이오리더스 | 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균 |
| KR101564084B1 (ko) | 2009-01-08 | 2015-10-29 | 주식회사 바이오리더스 | ｒｅｐＥ 변이유전자를 함유하는 항시적 고발현 벡터 |
| JP5599153B2 (ja) * | 2009-03-17 | 2014-10-01 | 花王株式会社 | ポリ−ガンマ−グルタミン酸の製造方法 |
| JP5669361B2 (ja) * | 2009-03-17 | 2015-02-12 | 花王株式会社 | ポリ−ガンマ−グルタミン酸の調整方法 |
| WO2011034181A1 (ja) | 2009-09-17 | 2011-03-24 | 森下仁丹株式会社 | ビフィズス菌表層提示融合タンパク質発現遺伝子 |
| JP5846523B2 (ja) * | 2010-11-18 | 2016-01-20 | 国立大学法人神戸大学 | βグルコシダーゼを発現する大腸菌 |
| JP2014210747A (ja) | 2013-04-19 | 2014-11-13 | アンジェスＭｇ株式会社 | 細胞性免疫誘導能が改善された経口ワクチン |
| CN103555720B (zh) * | 2013-11-19 | 2015-08-19 | 湖北工业大学 | 一种具有生产和回收纤维素内切酶双重功能的酵母菌株的构建 |
| US10695385B2 (en) | 2015-06-25 | 2020-06-30 | National University Corporation Kobe University | Oral cancer vaccine |
| US11666646B2 (en) | 2016-12-26 | 2023-06-06 | National University Corporation Kobe University | Cancer therapy utilizing combination of oral tumor vaccine and immunosuppression inhibitor |
| KR20200000972A (ko) * | 2018-06-26 | 2020-01-06 | 주식회사 바이오리더스 | 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를 이용한 표면발현벡터 및 이를 이용한 단백질의 미생물표면 발현 방법 |
| US12188027B2 (en) * | 2018-10-10 | 2025-01-07 | Bioleaders Corporation | Surface expression vector using two kinds of promoters derived from Lactobacillus casei for concurrently expressing two target proteins and method for expressing proteins on microbial surface by using same |
| US12180469B2 (en) * | 2018-10-10 | 2024-12-31 | Bioleaders Corporation | Surface expression vector for constitutive high-expression using promoter of galactose mutarotase gene derived from Lactobacillus casei, and use thereof |
| CN111593029B (zh) * | 2020-05-29 | 2022-05-17 | 江西省科学院微生物研究所 | 一种催化活性提高的γ-聚谷氨酸合成酶复合体及其编码基因 |
| CN113512562B (zh) * | 2021-04-28 | 2022-05-17 | 齐鲁工业大学 | γ-聚谷氨酸在植物中异源合成提高植物抗逆性和产量的方法 |
| CN114702559A (zh) * | 2022-04-02 | 2022-07-05 | 江西师范大学 | 枯草芽孢杆菌蛋白PgsA及其在表面展示系统中的应用 |
| KR20240053728A (ko) | 2022-10-17 | 2024-04-25 | 주식회사 퓨처앤텍 | 세포외막 지질단백질 PrsA 발현계를 이용한 세포표면 대량발현 기술 |
| CN118956803A (zh) * | 2022-11-22 | 2024-11-15 | 中国农业科学院茶叶研究所 | 茶树甲基化儿茶素合成酶CsFAOMT2、编码基因及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5356797A (en) * | 1991-11-15 | 1994-10-18 | Board Of Regents, The University Of Texas | Membrane expression of heterologous genes |
| US5348867A (en) | 1991-11-15 | 1994-09-20 | George Georgiou | Expression of proteins on bacterial surface |
| WO1993024636A1 (en) | 1992-05-29 | 1993-12-09 | The University Of British Columbia | Use of protein oprf for bacterial cell surface expression of oligopeptides |
| CA2148479A1 (en) | 1992-11-03 | 1994-05-11 | Staffan Normark | Fibronectin binding protein as well as its preparation |
| DE4243770A1 (de) * | 1992-12-23 | 1994-06-30 | Behringwerke Ag | Verfahren zur Herstellung von rekombinanten und synthetischen Peptiden und deren Verwendung |
| US5874239A (en) | 1993-07-30 | 1999-02-23 | Affymax Technologies N.V. | Biotinylation of proteins |
| FR2708622B1 (fr) | 1993-08-02 | 1997-04-18 | Raymond Hamers | Vecteur recombinant contenant une séquence d'un gène de lipoprotéine de structure pour l'expression de séquences de nucléotides. |
| KR0185335B1 (ko) * | 1996-04-02 | 1999-04-01 | 김은영 | 표면 발현 벡터 및 이를 이용한 단백질의 미생물 표면 발현 방법 |
| JP2001017182A (ja) | 1999-07-09 | 2001-01-23 | Nagase & Co Ltd | ポリ−γ−グルタミン酸の製造方法 |
-
2002
- 2002-08-09 JP JP2003519489A patent/JP4458844B2/ja not_active Expired - Fee Related
- 2002-08-09 CN CNB028153715A patent/CN1311082C/zh not_active Expired - Lifetime
- 2002-08-09 AT AT02755966T patent/ATE342990T1/de not_active IP Right Cessation
- 2002-08-09 WO PCT/KR2002/001522 patent/WO2003014360A1/en not_active Ceased
- 2002-08-09 US US10/484,605 patent/US7553636B2/en not_active Expired - Lifetime
- 2002-08-09 DE DE60215521T patent/DE60215521T2/de not_active Expired - Lifetime
- 2002-08-09 KR KR10-2003-7016665A patent/KR100469800B1/ko not_active Expired - Fee Related
- 2002-08-09 EP EP02755966A patent/EP1421195B1/en not_active Expired - Lifetime
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