CN1311082C - 具有编码多聚－γ－谷氨酸合成酶的基因pgsBCA的表面表达载体以及利用该载体在微生物表面表达目的蛋白的方法 - Google Patents

具有编码多聚－γ－谷氨酸合成酶的基因pgsBCA的表面表达载体以及利用该载体在微生物表面表达目的蛋白的方法 Download PDF

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CN1311082C
CN1311082C CNB028153715A CN02815371A CN1311082C CN 1311082 C CN1311082 C CN 1311082C CN B028153715 A CNB028153715 A CN B028153715A CN 02815371 A CN02815371 A CN 02815371A CN 1311082 C CN1311082 C CN 1311082C
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expression vector
gene
protein
antigen
gram
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CN1539018A (zh
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成文喜
洪承杓
李宗洙
郑昌敏
金哲仲
左右田健次
芦内诚
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MD Lab Co Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01133—Xylosylprotein 4-beta-galactosyltransferase (2.4.1.133)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08005—CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase (2.7.8.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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CNB028153715A 2001-08-10 2002-08-09 具有编码多聚－γ－谷氨酸合成酶的基因pgsBCA的表面表达载体以及利用该载体在微生物表面表达目的蛋白的方法 Expired - Lifetime CN1311082C (zh)

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KR20010048373 2001-08-10
KR200148373 2001-08-10

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CN1311082C true CN1311082C (zh) 2007-04-18

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US (1) US7553636B2 (enExample)
EP (1) EP1421195B1 (enExample)
JP (1) JP4458844B2 (enExample)
KR (1) KR100469800B1 (enExample)
CN (1) CN1311082C (enExample)
AT (1) ATE342990T1 (enExample)
DE (1) DE60215521T2 (enExample)
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AU2003271215A1 (en) 2002-10-17 2004-05-04 Bioleaders Corporation Vector for anti-hpv vaccine and transformed microorganism by the vector
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KR20050079162A (ko) * 2004-02-04 2005-08-09 주식회사 바이오리더스 파보바이러스 항원의 세포표면 발현벡터 및 상기 벡터에의해 형질전환된 미생물
US7255855B2 (en) * 2004-02-27 2007-08-14 Bioleaders Corporation Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase
JP2005312426A (ja) * 2004-03-31 2005-11-10 Bioleaders Japan:Kk タンパク質を表層提示する大腸菌
AU2006242770A1 (en) * 2005-03-04 2006-11-09 Suntory Holdings Limited Transgenic plant for producing polyglutamic acid
JP2006262724A (ja) * 2005-03-22 2006-10-05 Kobe Univ タンパク質高発現乳酸菌
WO2006126682A1 (ja) * 2005-05-27 2006-11-30 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute アルツハイマー病の予防・治療用ワクチン
KR100872042B1 (ko) * 2005-09-14 2008-12-05 주식회사 바이오리더스 마이오스타틴을 발현하는 세포표면 발현벡터 및 상기벡터에 의해 형질전환된 미생물
KR100578395B1 (ko) * 2005-11-11 2006-05-10 주식회사 바이오리더스 면역기능이 강화된 사균화 유산균 제제 및 그 제조방법
KR100782332B1 (ko) * 2006-01-23 2007-12-06 주식회사 바이오리더스 새우 흰 반점 바이러스 항원의 표면 발현벡터 및 이에 의해형질전환된 미생물
KR100985914B1 (ko) * 2006-12-13 2010-10-08 주식회사 바이오리더스 간암 특이 항원인 알파­페토단백질의 표면발현벡터 및 이에의해 형질전환된 미생물
KR100972843B1 (ko) * 2007-03-22 2010-07-28 주식회사 바이오리더스 신규 항시적 고발현 프로모터 및 그 용도
KR100857861B1 (ko) * 2007-10-15 2008-09-11 주식회사 바이오리더스 Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물
KR101471043B1 (ko) 2009-01-08 2014-12-09 주식회사 바이오리더스 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균
KR101564084B1 (ko) 2009-01-08 2015-10-29 주식회사 바이오리더스 ｒｅｐＥ 변이유전자를 함유하는 항시적 고발현 벡터
JP5599153B2 (ja) * 2009-03-17 2014-10-01 花王株式会社 ポリ−ガンマ−グルタミン酸の製造方法
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CN103555720B (zh) * 2013-11-19 2015-08-19 湖北工业大学 一种具有生产和回收纤维素内切酶双重功能的酵母菌株的构建
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KR20200000972A (ko) * 2018-06-26 2020-01-06 주식회사 바이오리더스 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를 이용한 표면발현벡터 및 이를 이용한 단백질의 미생물표면 발현 방법
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CN113512562B (zh) * 2021-04-28 2022-05-17 齐鲁工业大学 γ-聚谷氨酸在植物中异源合成提高植物抗逆性和产量的方法
CN114702559A (zh) * 2022-04-02 2022-07-05 江西师范大学 枯草芽孢杆菌蛋白PgsA及其在表面展示系统中的应用
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DE60215521T2 (de) 2007-06-21
KR100469800B1 (ko) 2005-02-02
EP1421195A1 (en) 2004-05-26
EP1421195A4 (en) 2005-04-27
WO2003014360A1 (en) 2003-02-20
US20040253704A1 (en) 2004-12-16
ATE342990T1 (de) 2006-11-15
CN1539018A (zh) 2004-10-20
JP4458844B2 (ja) 2010-04-28
KR20040032824A (ko) 2004-04-17
EP1421195B1 (en) 2006-10-18
US7553636B2 (en) 2009-06-30
DE60215521D1 (de) 2006-11-30
JP2005500054A (ja) 2005-01-06

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