JP2004536617A - 核酸増幅方法 - Google Patents
核酸増幅方法 Download PDFInfo
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- JP2004536617A JP2004536617A JP2003517293A JP2003517293A JP2004536617A JP 2004536617 A JP2004536617 A JP 2004536617A JP 2003517293 A JP2003517293 A JP 2003517293A JP 2003517293 A JP2003517293 A JP 2003517293A JP 2004536617 A JP2004536617 A JP 2004536617A
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0118959A GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
| PCT/SE2002/001378 WO2003012119A2 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008316989A Division JP4559517B2 (ja) | 2001-08-03 | 2008-12-12 | 核酸増幅方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2004536617A true JP2004536617A (ja) | 2004-12-09 |
Family
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Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003517293A Pending JP2004536617A (ja) | 2001-08-03 | 2002-07-12 | 核酸増幅方法 |
| JP2008316989A Expired - Lifetime JP4559517B2 (ja) | 2001-08-03 | 2008-12-12 | 核酸増幅方法 |
| JP2009230088A Expired - Lifetime JP4675424B2 (ja) | 2001-08-03 | 2009-10-02 | 核酸増幅方法 |
| JP2010125694A Expired - Lifetime JP5313204B2 (ja) | 2001-08-03 | 2010-06-01 | 核酸増幅方法 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008316989A Expired - Lifetime JP4559517B2 (ja) | 2001-08-03 | 2008-12-12 | 核酸増幅方法 |
| JP2009230088A Expired - Lifetime JP4675424B2 (ja) | 2001-08-03 | 2009-10-02 | 核酸増幅方法 |
| JP2010125694A Expired - Lifetime JP5313204B2 (ja) | 2001-08-03 | 2010-06-01 | 核酸増幅方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7320860B2 (https=) |
| EP (4) | EP2251439B1 (https=) |
| JP (4) | JP2004536617A (https=) |
| AU (1) | AU2002314693A1 (https=) |
| CA (1) | CA2455803A1 (https=) |
| GB (1) | GB2378245A (https=) |
| WO (1) | WO2003012119A2 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008522628A (ja) * | 2004-12-11 | 2008-07-03 | サイトジェニックス， インコーポレイテッド | 高品質核酸のセルフリー生合成及びその使用 |
| JP2009077735A (ja) * | 2001-08-03 | 2009-04-16 | Biocyclica Ab | 核酸増幅方法 |
| JP2011505161A (ja) * | 2007-12-04 | 2011-02-24 | ピエール、ファーブル、メディカマン | 抗体ライブラリーを作製およびスクリーニングするための新規な方法 |
Families Citing this family (116)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0304371D0 (en) * | 2003-02-26 | 2003-04-02 | Solexa Ltd | DNA Sequence analysis |
| US7385043B1 (en) * | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| US7618780B2 (en) * | 2004-05-20 | 2009-11-17 | Trillion Genomics Limited | Use of mass labelled probes to detect target nucleic acids using mass spectrometry |
| CA2577079C (en) * | 2004-08-13 | 2014-05-20 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore dna readout platform |
| JP5103398B2 (ja) * | 2005-06-06 | 2012-12-19 | ４５４ ライフ サイエンシーズ コーポレイション | 両末端配列決定（ｐａｉｒｅｄｅｎｄｓｅｑｕｅｎｃｉｎｇ） |
| US7601499B2 (en) | 2005-06-06 | 2009-10-13 | 454 Life Sciences Corporation | Paired end sequencing |
| JP5331476B2 (ja) | 2005-06-15 | 2013-10-30 | カリダ・ジェノミックス・インコーポレイテッド | 遺伝子解析および化学解析用の単分子アレイ |
| US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
| US8492098B2 (en) | 2006-02-21 | 2013-07-23 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of reaction components that affect a reaction |
| SG170028A1 (en) | 2006-02-24 | 2011-04-29 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090075343A1 (en) | 2006-11-09 | 2009-03-19 | Complete Genomics, Inc. | Selection of dna adaptor orientation by nicking |
| US7862999B2 (en) | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
| WO2008122960A2 (en) * | 2007-04-10 | 2008-10-16 | Microvacuum Ltd. | Label-free optical detection method |
| US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
| AU2008308457A1 (en) * | 2007-10-04 | 2009-04-09 | Halcyon Molecular | Sequencing nucleic acid polymers with electron microscopy |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| WO2009073629A2 (en) | 2007-11-29 | 2009-06-11 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US20090233809A1 (en) * | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
| US8470542B2 (en) * | 2008-12-01 | 2013-06-25 | Laboratory Corporation Of America Holdings | Methods and assays for measuring p95 and/or p95 complexes in a sample and antibodies specific for p95 |
| SG172983A1 (en) | 2009-01-15 | 2011-08-29 | Lab Corp America Holdings | Methods of determining patient response by measurement of her-3 |
| SG172984A1 (en) * | 2009-01-15 | 2011-08-29 | Lab Corp America Holdings | Methods of determining patient response by measurement of her-2 expression |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| EP2425240A4 (en) | 2009-04-30 | 2012-12-12 | Good Start Genetics Inc | METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
| JP2013511991A (ja) * | 2009-11-25 | 2013-04-11 | クアンタライフ， インコーポレイテッド | 遺伝子材料を検出する方法および組成物 |
| WO2011109379A1 (en) | 2010-03-01 | 2011-09-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| WO2011161549A2 (en) | 2010-06-24 | 2011-12-29 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
| EP2627781B1 (en) | 2010-10-15 | 2017-02-22 | Olink Bioscience AB | Dynamic range methods |
| CN101974638B (zh) * | 2010-11-10 | 2012-10-03 | 华东医学生物技术研究所 | 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法 |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| AU2012214312A1 (en) | 2011-02-09 | 2013-08-22 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| EP2675916B1 (en) | 2011-02-15 | 2016-08-24 | Leica Biosystems Newcastle Limited | METHOD FOR LOCALIZED IN SITU DETECTION OF mRNA |
| US9556473B2 (en) | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| US10597701B2 (en) | 2011-05-11 | 2020-03-24 | Navinci Diagnostics Ab | Unfolding proximity probes and methods for the use thereof |
| CA2852665A1 (en) | 2011-10-17 | 2013-04-25 | Good Start Genetics, Inc. | Analysis methods |
| US9670529B2 (en) | 2012-02-28 | 2017-06-06 | Population Genetics Technologies Ltd. | Method for attaching a counter sequence to a nucleic acid sample |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| KR101922124B1 (ko) | 2012-08-27 | 2018-11-26 | 삼성전자주식회사 | 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법 |
| WO2014076209A1 (en) | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| WO2014076214A1 (en) | 2012-11-14 | 2014-05-22 | Olink Ab | Rca reporter probes and their use in detecting nucleic acid molecules |
| JP6112652B2 (ja) * | 2012-11-29 | 2017-04-12 | 国立大学法人広島大学 | 標的ｄｎａの特異的増幅方法 |
| EP3988671A1 (en) * | 2013-02-20 | 2022-04-27 | Emory University | Compositions for sequencing nucleic acids in mixtures |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CA2904181A1 (en) | 2013-03-13 | 2014-10-09 | Anahit Aghvanyan | Sandwich immunoassay comprising anchoring reagent |
| WO2014152421A1 (en) | 2013-03-14 | 2014-09-25 | Good Start Genetics, Inc. | Methods for analyzing nucleic acids |
| KR102134168B1 (ko) | 2013-03-15 | 2020-07-15 | 루브리졸 어드밴스드 머티어리얼스, 인코포레이티드 | 중금속이 없는 씨피브이씨 조성물 |
| US8847799B1 (en) | 2013-06-03 | 2014-09-30 | Good Start Genetics, Inc. | Methods and systems for storing sequence read data |
| WO2015013681A1 (en) | 2013-07-25 | 2015-01-29 | Bio-Rad Laboratories, Inc. | Genetic assays |
| US9217167B2 (en) | 2013-07-26 | 2015-12-22 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| US9644232B2 (en) | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| US11041203B2 (en) | 2013-10-18 | 2021-06-22 | Molecular Loop Biosolutions, Inc. | Methods for assessing a genomic region of a subject |
| US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
| GB201321123D0 (en) * | 2013-11-29 | 2014-01-15 | Linea Ab Q | Amplification of circular molecules |
| GB2520765A (en) | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Multiplex detection of nucleic acids |
| US10112194B2 (en) | 2014-04-14 | 2018-10-30 | Q-Linea Ab | Detection of microscopic objects |
| US11053548B2 (en) | 2014-05-12 | 2021-07-06 | Good Start Genetics, Inc. | Methods for detecting aneuploidy |
| WO2015175856A1 (en) | 2014-05-15 | 2015-11-19 | Meso Scale Technologies, Llc. | Improved assay methods |
| KR102535489B1 (ko) | 2014-06-13 | 2023-05-22 | 큐-리네아 에이비 | 미생물 검출 방법 및 특성 규명 방법 |
| GB201413718D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| GB201413717D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| US20160048608A1 (en) | 2014-08-15 | 2016-02-18 | Good Start Genetics, Inc. | Systems and methods for genetic analysis |
| US11408024B2 (en) | 2014-09-10 | 2022-08-09 | Molecular Loop Biosciences, Inc. | Methods for selectively suppressing non-target sequences |
| US10429399B2 (en) | 2014-09-24 | 2019-10-01 | Good Start Genetics, Inc. | Process control for increased robustness of genetic assays |
| EP3209798B1 (en) | 2014-10-23 | 2022-07-27 | Ricardo Mancebo | Reagents and methods for isothermal chain reaction |
| EP4095261B1 (en) | 2015-01-06 | 2025-05-28 | Molecular Loop Biosciences, Inc. | Screening for structural variants |
| GB201507026D0 (en) | 2015-04-24 | 2015-06-10 | Linea Ab Q | Medical sample transportation container |
| EP4219532A3 (en) | 2015-06-05 | 2023-08-16 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for transient gene therapy with enhanced stability |
| JP6698708B2 (ja) | 2015-06-09 | 2020-05-27 | ライフ テクノロジーズ コーポレーション | 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 |
| GB201511129D0 (en) | 2015-06-24 | 2015-08-05 | Linea Ab Q | Method of determining antimicrobial susceptibility of a microorganism |
| ES2745556T3 (es) | 2015-07-29 | 2020-03-02 | Progenity Inc | Acidos nucleicos y métodos para detectar anomalías cromosómicas |
| GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| CA3005101A1 (en) | 2015-11-16 | 2017-05-26 | Progenity, Inc. | Nucleic acids and methods for detecting methylation status |
| WO2017173279A1 (en) * | 2016-03-31 | 2017-10-05 | Arizona Board Of Regents On Behalf Of Arizona State University | Probes and methods for measuring tandem repeats |
| GB2554767A (en) | 2016-04-21 | 2018-04-11 | Q Linea Ab | Detecting and characterising a microorganism |
| US10655170B2 (en) | 2016-07-06 | 2020-05-19 | Takara Bio Usa, Inc. | Coupling adaptors to a target nucleic acid |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| GB201621514D0 (en) | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
| US11661624B2 (en) | 2017-03-30 | 2023-05-30 | Pioneer Hi-Bred International, Inc. | Methods of identifying and characterizing gene editing variations in nucleic acids |
| US11753434B2 (en) | 2017-04-14 | 2023-09-12 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for transient gene therapy with enhanced stability |
| JP7240733B2 (ja) * | 2017-05-23 | 2023-03-16 | ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー | デュアル相互作用ヘアピンプローブを使用した標的媒介ｉｎ ｓｉｔｕシグナル増幅 |
| EP3682025A1 (en) | 2017-09-14 | 2020-07-22 | H. Hoffnabb-La Roche Ag | Novel method for generating circular single-stranded dna libraries |
| WO2019053215A1 (en) | 2017-09-15 | 2019-03-21 | F. Hoffmann-La Roche Ag | HYBRIDIZATION-EXTENSION-LIGATURE STRATEGY TO GENERATE CIRCULAR SINGLE-STRAND DNA BANKS |
| CA3078158A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | Rna templated ligation |
| EP3692166A1 (en) | 2017-10-06 | 2020-08-12 | H. Hoffnabb-La Roche Ag | Circularization methods for single molecule sequencing sample preparation |
| WO2019086531A1 (en) | 2017-11-03 | 2019-05-09 | F. Hoffmann-La Roche Ag | Linear consensus sequencing |
| EP3749782B1 (en) | 2018-02-05 | 2022-11-02 | F. Hoffmann-La Roche AG | Generation of single-stranded circular dna templates for single molecule sequencing |
| US11898204B2 (en) | 2018-03-02 | 2024-02-13 | Roche Sequencing Solutions, Inc. | Generation of single-stranded circular DNA templates for single molecule sequencing |
| WO2019195346A1 (en) | 2018-04-02 | 2019-10-10 | Progenity, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| CN109444102B (zh) * | 2018-12-18 | 2021-03-23 | 济南大学 | 一种检测赭曲霉毒素a的荧光生物传感器及其制备方法和应用 |
| AU2020232954B2 (en) | 2019-03-01 | 2026-01-15 | Meso Scale Technologies, Llc. | Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same |
| EP3947718A4 (en) | 2019-04-02 | 2022-12-21 | Enumera Molecular, Inc. | METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES |
| JP7727628B2 (ja) | 2019-12-03 | 2025-08-21 | アラマー バイオサイエンシーズ， インク． | 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ） |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| EP4121561A4 (en) | 2020-08-06 | 2024-04-17 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| WO2022032195A2 (en) | 2020-08-06 | 2022-02-10 | Singular Genomics Systems, Inc. | Spatial sequencing |
| WO2022056078A1 (en) * | 2020-09-11 | 2022-03-17 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Rnase h-assisted detection assay for rna (radar) |
| CN116438454A (zh) | 2020-09-21 | 2023-07-14 | 普罗根尼蒂公司 | 用于分离细胞游离dna的组合物和方法 |
| US12497651B2 (en) | 2020-11-23 | 2025-12-16 | Pleno, Inc. | Encoded dual-probe endonuclease assays |
| WO2022235764A1 (en) | 2021-05-05 | 2022-11-10 | Singular Genomics Systems, Inc. | Multiomic analysis device and methods of use thereof |
| GB202203185D0 (en) | 2022-03-08 | 2022-04-20 | Rarity Bioscience Ab | Method for detecting nucleic acid amplification products |
| EP4720332A1 (en) | 2023-06-01 | 2026-04-08 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| GB202310278D0 (en) | 2023-07-05 | 2023-08-16 | Navinci Diagnostics Ab | Stabilisation of phi29 polymerase |
| CN120249442B (zh) * | 2025-06-04 | 2025-09-26 | 北京航空航天大学杭州创新研究院 | 一种基于邻近核酸扩增信号放大的超灵敏免疫检测方法 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| AU649066B2 (en) * | 1990-07-25 | 1994-05-12 | Syngene, Inc. | Circular extension for generating multiple nucleic acid complements |
| JP3085409B2 (ja) | 1991-03-29 | 2000-09-11 | 東洋紡績株式会社 | 標的核酸配列の検出方法およびそのための試薬キット |
| US5714320A (en) | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US6077668A (en) * | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
| WO1995014106A2 (en) | 1993-11-17 | 1995-05-26 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
| WO1996020287A2 (en) | 1994-12-23 | 1996-07-04 | Behringwerke Aktiengesellschaft | Detection of nucleic acids by target-catalyzed product formation |
| SE504798C2 (sv) | 1995-06-16 | 1997-04-28 | Ulf Landegren | Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| AU714486B2 (en) | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
| ATE324464T1 (de) | 1995-12-05 | 2006-05-15 | Jorn Erland Koch | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| EP1985714B1 (en) | 1998-03-25 | 2012-02-29 | Olink AB | Method and kit for detecting a target molecule employing at least two affinity probes and rolling circle replication of padlock probes |
| US6037130A (en) * | 1998-07-28 | 2000-03-14 | The Public Health Institute Of The City Of New York, Inc. | Wavelength-shifting probes and primers and their use in assays and kits |
| US6150112A (en) | 1998-09-18 | 2000-11-21 | Yale University | Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors |
| US6235502B1 (en) * | 1998-09-18 | 2001-05-22 | Molecular Staging Inc. | Methods for selectively isolating DNA using rolling circle amplification |
| AU781962B2 (en) | 1998-12-15 | 2005-06-23 | Qiagen Gmbh | A method of amplification of a circularised nucleic acid probe |
| US6830884B1 (en) * | 1998-12-15 | 2004-12-14 | Molecular Staging Inc. | Method of amplification |
| US6274320B1 (en) * | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| JP2003515149A (ja) * | 1999-11-26 | 2003-04-22 | キュラジェン コーポレイション | 核酸プローブアレイ |
| WO2001040516A2 (en) | 1999-12-02 | 2001-06-07 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
| US6221603B1 (en) * | 2000-02-04 | 2001-04-24 | Molecular Dynamics, Inc. | Rolling circle amplification assay for nucleic acid analysis |
| US6291187B1 (en) | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| US6573051B2 (en) * | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| SE0401270D0 (sv) | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
-
2001
- 2001-08-03 GB GB0118959A patent/GB2378245A/en not_active Withdrawn
-
2002
- 2002-07-12 CA CA002455803A patent/CA2455803A1/en not_active Abandoned
- 2002-07-12 AU AU2002314693A patent/AU2002314693A1/en not_active Abandoned
- 2002-07-12 EP EP10003586.4A patent/EP2251439B1/en not_active Expired - Lifetime
- 2002-07-12 JP JP2003517293A patent/JP2004536617A/ja active Pending
- 2002-07-12 EP EP10003679.7A patent/EP2236622B1/en not_active Expired - Lifetime
- 2002-07-12 EP EP10003678A patent/EP2224016A1/en not_active Withdrawn
- 2002-07-12 WO PCT/SE2002/001378 patent/WO2003012119A2/en not_active Ceased
- 2002-07-12 US US10/483,900 patent/US7320860B2/en not_active Expired - Lifetime
- 2002-07-12 EP EP02741612.2A patent/EP1423532B1/en not_active Expired - Lifetime
-
2007
- 2007-11-28 US US11/946,706 patent/US7790388B2/en not_active Ceased
-
2008
- 2008-12-12 JP JP2008316989A patent/JP4559517B2/ja not_active Expired - Lifetime
-
2009
- 2009-10-02 JP JP2009230088A patent/JP4675424B2/ja not_active Expired - Lifetime
-
2010
- 2010-06-01 JP JP2010125694A patent/JP5313204B2/ja not_active Expired - Lifetime
-
2011
- 2011-12-19 US US13/329,998 patent/USRE44265E1/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009077735A (ja) * | 2001-08-03 | 2009-04-16 | Biocyclica Ab | 核酸増幅方法 |
| JP2008522628A (ja) * | 2004-12-11 | 2008-07-03 | サイトジェニックス， インコーポレイテッド | 高品質核酸のセルフリー生合成及びその使用 |
| JP2011505161A (ja) * | 2007-12-04 | 2011-02-24 | ピエール、ファーブル、メディカマン | 抗体ライブラリーを作製およびスクリーニングするための新規な方法 |
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| EP2224016A1 (en) | 2010-09-01 |
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| JP4559517B2 (ja) | 2010-10-06 |
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| JP4675424B2 (ja) | 2011-04-20 |
| JP2009297045A (ja) | 2009-12-24 |
| EP2236622B1 (en) | 2017-09-06 |
| US7790388B2 (en) | 2010-09-07 |
| EP2236622A3 (en) | 2010-11-03 |
| AU2002314693A1 (en) | 2003-02-17 |
| EP2251439B1 (en) | 2013-09-11 |
| EP1423532A2 (en) | 2004-06-02 |
| EP2251439A1 (en) | 2010-11-17 |
| US7320860B2 (en) | 2008-01-22 |
| USRE44265E1 (en) | 2013-06-04 |
| GB2378245A (en) | 2003-02-05 |
| JP2009077735A (ja) | 2009-04-16 |
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