JP2003526344A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2003526344A5 JP2003526344A5 JP2001561783A JP2001561783A JP2003526344A5 JP 2003526344 A5 JP2003526344 A5 JP 2003526344A5 JP 2001561783 A JP2001561783 A JP 2001561783A JP 2001561783 A JP2001561783 A JP 2001561783A JP 2003526344 A5 JP2003526344 A5 JP 2003526344A5
- Authority
- JP
- Japan
- Prior art keywords
- oligonucleotide
- nucleic acid
- strand
- extendable
- template strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 description 80
- 108091034117 Oligonucleotide Proteins 0.000 description 71
- 150000007523 nucleic acids Chemical class 0.000 description 55
- 108020004707 nucleic acids Proteins 0.000 description 53
- 102000039446 nucleic acids Human genes 0.000 description 53
- 239000002773 nucleotide Substances 0.000 description 30
- 125000003729 nucleotide group Chemical group 0.000 description 30
- 238000006116 polymerization reaction Methods 0.000 description 30
- 235000011178 triphosphate Nutrition 0.000 description 21
- 239000001226 triphosphate Substances 0.000 description 21
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 description 18
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 16
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 16
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 16
- 235000011180 diphosphates Nutrition 0.000 description 16
- 229940048084 pyrophosphate Drugs 0.000 description 16
- 230000000295 complement effect Effects 0.000 description 15
- 230000003321 amplification Effects 0.000 description 14
- 238000003199 nucleic acid amplification method Methods 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 12
- 239000002777 nucleoside Substances 0.000 description 10
- -1 nucleoside triphosphate Chemical class 0.000 description 10
- 230000003213 activating effect Effects 0.000 description 9
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 230000000379 polymerizing effect Effects 0.000 description 7
- 238000000137 annealing Methods 0.000 description 6
- 108700028369 Alleles Proteins 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 239000005546 dideoxynucleotide Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 108010006785 Taq Polymerase Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 3
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 230000000865 phosphorylative effect Effects 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- RSEBUVRVKCANEP-UHFFFAOYSA-N 2-pyrroline Chemical compound C1CC=CN1 RSEBUVRVKCANEP-UHFFFAOYSA-N 0.000 description 2
- 102000009609 Pyrophosphatases Human genes 0.000 description 2
- 108010009413 Pyrophosphatases Proteins 0.000 description 2
- 230000037429 base substitution Effects 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000011223 gene expression profiling Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229940005657 pyrophosphoric acid Drugs 0.000 description 2
- ZVJHJDDKYZXRJI-UHFFFAOYSA-N pyrroline Natural products C1CC=NC1 ZVJHJDDKYZXRJI-UHFFFAOYSA-N 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 2
- RAJMXAZJKUGYGW-POYBYMJQSA-N 2',3'-dideoxycytidine-5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)CC1 RAJMXAZJKUGYGW-POYBYMJQSA-N 0.000 description 1
- WVNRRNJFRREKAR-JGVFFNPUSA-N 2',3'-dideoxythymidine-5'-monophosphate Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)CC1 WVNRRNJFRREKAR-JGVFFNPUSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 101100545180 Mus musculus Zc3h12d gene Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- PUSXDQXVJDGIBK-NKWVEPMBSA-N [(2s,5r)-5-(6-aminopurin-9-yl)oxolan-2-yl]methyl dihydrogen phosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](COP(O)(O)=O)O1 PUSXDQXVJDGIBK-NKWVEPMBSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001841 pyrophosphorolytic effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18431500P | 2000-02-23 | 2000-02-23 | |
| US60/184,315 | 2000-02-23 | ||
| US18703500P | 2000-03-06 | 2000-03-06 | |
| US60/187,035 | 2000-03-06 | ||
| US23718000P | 2000-10-03 | 2000-10-03 | |
| US60/237,180 | 2000-10-03 | ||
| PCT/US2001/005527 WO2001062975A2 (en) | 2000-02-23 | 2001-02-22 | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003526344A JP2003526344A (ja) | 2003-09-09 |
| JP2003526344A5 true JP2003526344A5 (https=) | 2008-04-17 |
| JP4989004B2 JP4989004B2 (ja) | 2012-08-01 |
Family
ID=27391815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001561783A Expired - Fee Related JP4989004B2 (ja) | 2000-02-23 | 2001-02-22 | 加ピロリン酸分解活性化重合（ｐａｐ）：アリル−特異的増幅および核酸配列決定への適応 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US6534269B2 (https=) |
| EP (2) | EP1259646B1 (https=) |
| JP (1) | JP4989004B2 (https=) |
| AT (1) | ATE441726T1 (https=) |
| AU (2) | AU2001241621B2 (https=) |
| CA (1) | CA2395391C (https=) |
| DE (1) | DE60139762D1 (https=) |
| ES (1) | ES2330408T3 (https=) |
| WO (1) | WO2001062975A2 (https=) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| AU2001291513B2 (en) * | 2000-10-03 | 2007-06-07 | Id+Plus Ltd | Nucleotide detection method |
| AUPR050700A0 (en) | 2000-10-03 | 2000-10-26 | Id+Plus Ltd | Detection method |
| US7211414B2 (en) * | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| JP2004526448A (ja) * | 2001-03-30 | 2004-09-02 | アプレラ コーポレイション | 非鋳型ヌクレオチド付加を用いた核酸分析 |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| AU2002367744A1 (en) * | 2001-07-03 | 2003-10-27 | The Board Of Trustees Of The Leland Stanford University | Bioluminescence regenerative cycle (brc) for nucleic acid quantification |
| US7668697B2 (en) * | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
| EP1509619B1 (en) * | 2002-05-10 | 2012-04-04 | City of Hope | Pyrophosphorolysis activated polymerization (pap) |
| WO2005086679A2 (en) * | 2004-03-05 | 2005-09-22 | Medical College Of Ohio | Methods and compositions for assessing nucleic acids and alleles |
| WO2005106036A2 (en) | 2004-04-12 | 2005-11-10 | Medical College Of Ohio | Methods and compositions for assaying analytes |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US20060060977A1 (en) * | 2004-09-22 | 2006-03-23 | Kabushiki Kaisha Toshiba | Semiconductor device |
| US7378260B2 (en) * | 2005-04-01 | 2008-05-27 | Applera Corporation | Products and methods for reducing dye artifacts |
| ES2553893T3 (es) * | 2007-11-28 | 2015-12-14 | F. Hoffmann-La Roche Ag | ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) |
| DK2279263T3 (da) | 2008-04-30 | 2013-11-11 | Integrated Dna Tech Inc | Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CN101381766B (zh) * | 2008-06-11 | 2012-04-18 | 厦门大学 | 一种基因稀有突变的定量检测方法 |
| US20100112565A1 (en) * | 2008-11-03 | 2010-05-06 | Life Technologies Corporation | Methods, kits, and reaction mixtures for high resolution melt genotyping |
| US8481685B2 (en) * | 2008-11-03 | 2013-07-09 | Kapa Biosystems | Modified DNA polymerases |
| US8932813B2 (en) | 2010-12-13 | 2015-01-13 | Life Technologies Corporation | Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions |
| CA3002752A1 (en) | 2015-10-26 | 2017-05-04 | Oti Lumionics Inc. | Method for patterning a coating on a surface and device including a patterned coating |
| CN105316421B (zh) * | 2015-12-01 | 2019-09-03 | 湖南宏雅基因技术有限公司 | 用于检测与肺癌相关的shox2基因启动子区甲基化程度的试剂盒 |
| CN105331727B (zh) * | 2015-12-01 | 2019-09-03 | 湖南宏雅基因技术有限公司 | 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒 |
| CN118215324B (zh) | 2016-12-02 | 2025-08-15 | Oti照明公司 | 包括设置在发射区域上面的导电涂层的器件及其方法 |
| CN116583131A (zh) | 2017-04-26 | 2023-08-11 | Oti照明公司 | 用于图案化表面上覆层的方法和包括图案化覆层的装置 |
| WO2018211460A1 (en) | 2017-05-17 | 2018-11-22 | Oti Lumionics Inc. | Method for selectively depositing a conductive coating over a patterning coating and device including a conductive coating |
| WO2019062614A1 (en) * | 2017-09-26 | 2019-04-04 | Phocus Technology ( Shanghai) Co. Ltd | METHOD FOR AMPLIFYING TARGET NUCLEIC ACID |
| US11751415B2 (en) | 2018-02-02 | 2023-09-05 | Oti Lumionics Inc. | Materials for forming a nucleation-inhibiting coating and devices incorporating same |
| ES2969883T3 (es) * | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| WO2019215591A1 (en) | 2018-05-07 | 2019-11-14 | Oti Lumionics Inc. | Method for providing an auxiliary electrode and device including an auxiliary electrode |
| US11268140B2 (en) * | 2018-06-12 | 2022-03-08 | Shaofeng Ding | Delayed pyrophosphorolysis activated polymerization |
| JP2022508040A (ja) | 2018-11-23 | 2022-01-19 | オーティーアイ ルミオニクス インコーポレーテッド | 光透過領域を含むオプトエレクトロニクスデバイス |
| KR20250160226A (ko) | 2019-03-07 | 2025-11-11 | 오티아이 루미오닉스 인크. | 핵생성 억제 코팅물 형성용 재료 및 이를 포함하는 디바이스 |
| CN113950630A (zh) | 2019-04-18 | 2022-01-18 | Oti照明公司 | 用于形成成核抑制涂层的材料和结合所述成核抑制涂层的装置 |
| US12069938B2 (en) | 2019-05-08 | 2024-08-20 | Oti Lumionics Inc. | Materials for forming a nucleation-inhibiting coating and devices incorporating same |
| KR20240134240A (ko) | 2019-06-26 | 2024-09-06 | 오티아이 루미오닉스 인크. | 광 회절 특성을 갖는 광 투과 영역을 포함하는 광전자 디바이스 |
| US11832473B2 (en) | 2019-06-26 | 2023-11-28 | Oti Lumionics Inc. | Optoelectronic device including light transmissive regions, with light diffraction characteristics |
| CN114342068A (zh) | 2019-08-09 | 2022-04-12 | Oti照明公司 | 包含辅助电极和分区的光电子装置 |
| KR102931641B1 (ko) | 2019-12-24 | 2026-02-26 | 오티아이 루미오닉스 인크. | 캡핑 층을 포함하는 발광 장치 및 그 제조방법 |
| CN111108860B (zh) * | 2020-01-19 | 2024-05-14 | 黑龙江丰沃非凡农业科技发展有限公司 | 一种仿形播种机 |
| US11985841B2 (en) | 2020-12-07 | 2024-05-14 | Oti Lumionics Inc. | Patterning a conductive deposited layer using a nucleation inhibiting coating and an underlying metallic coating |
| GB202102170D0 (en) * | 2021-02-16 | 2021-03-31 | Biofidelity Ltd | Polynucleotide detection |
| CN117904281B (zh) * | 2024-01-29 | 2024-11-22 | 上海科亦生物科技有限公司 | 检测ugt1a1基因多态性的引物组及试剂盒与方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| DE69118930T2 (de) * | 1990-01-26 | 1997-01-09 | Abbott Lab | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6335162B1 (en) * | 1998-03-13 | 2002-01-01 | Promega Corporation | Nucleic acid detection |
| US7090975B2 (en) | 1998-03-13 | 2006-08-15 | Promega Corporation | Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection |
| US6312902B1 (en) * | 1998-03-13 | 2001-11-06 | Promega Corporation | Nucleic acid detection |
| US6270974B1 (en) * | 1998-03-13 | 2001-08-07 | Promega Corporation | Exogenous nucleic acid detection |
| US6268146B1 (en) * | 1998-03-13 | 2001-07-31 | Promega Corporation | Analytical methods and materials for nucleic acid detection |
| US6270973B1 (en) * | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
| US6391551B1 (en) * | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
| US6277578B1 (en) * | 1998-03-13 | 2001-08-21 | Promega Corporation | Deploymerization method for nucleic acid detection of an amplified nucleic acid target |
| US6235480B1 (en) * | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
| US6159693A (en) * | 1998-03-13 | 2000-12-12 | Promega Corporation | Nucleic acid detection |
| US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| US6509157B1 (en) * | 1999-11-05 | 2003-01-21 | Roche Molecular Systems, Inc | 3 blocked nucleic acid amplification primers |
| WO2001075139A1 (en) * | 2000-04-03 | 2001-10-11 | Biolink Partners, Inc. | Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization |
-
2001
- 2001-02-22 AU AU2001241621A patent/AU2001241621B2/en not_active Ceased
- 2001-02-22 JP JP2001561783A patent/JP4989004B2/ja not_active Expired - Fee Related
- 2001-02-22 EP EP01912883A patent/EP1259646B1/en not_active Expired - Lifetime
- 2001-02-22 EP EP09166826.9A patent/EP2112233B1/en not_active Expired - Lifetime
- 2001-02-22 DE DE60139762T patent/DE60139762D1/de not_active Expired - Lifetime
- 2001-02-22 ES ES01912883T patent/ES2330408T3/es not_active Expired - Lifetime
- 2001-02-22 AU AU4162101A patent/AU4162101A/xx active Pending
- 2001-02-22 AT AT01912883T patent/ATE441726T1/de not_active IP Right Cessation
- 2001-02-22 US US09/789,556 patent/US6534269B2/en not_active Expired - Lifetime
- 2001-02-22 CA CA2395391A patent/CA2395391C/en not_active Expired - Fee Related
- 2001-02-22 WO PCT/US2001/005527 patent/WO2001062975A2/en not_active Ceased
-
2002
- 2002-10-15 US US10/269,879 patent/US7105298B2/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2003526344A5 (https=) | ||
| JP4989004B2 (ja) | 加ピロリン酸分解活性化重合（ｐａｐ）：アリル−特異的増幅および核酸配列決定への適応 | |
| Wu et al. | Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia. | |
| AU2001241621A1 (en) | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination | |
| AU2012304520B2 (en) | Circularized templates for sequencing | |
| US5066584A (en) | Methods for generating single stranded dna by the polymerase chain reaction | |
| AU753661B2 (en) | A method for the characterisation of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site | |
| US20040126760A1 (en) | Novel compositions and methods for carrying out multple pcr reactions on a single sample | |
| JP2002502615A5 (https=) | ||
| KR20010005544A (ko) | Ｖｎｔｒ 대립 유전자의 추출 및 이용방법 | |
| JPH11512607A (ja) | 核酸分子を特性化する方法 | |
| JP2010519896A5 (https=) | ||
| US20070207494A1 (en) | Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides | |
| JP2005511096A5 (https=) | ||
| WO2009154733A2 (en) | Polymerase incorporation of non-standard nucleotides | |
| JP2021517556A (ja) | 核酸ライブラリー作製のための組成物及び方法 | |
| WO2019062614A1 (en) | METHOD FOR AMPLIFYING TARGET NUCLEIC ACID | |
| CA2485596A1 (en) | Pyrophosphorolysis activated polymerization (pap) | |
| WO2001088174A1 (en) | Novel compositions and methods for carrying out multiple pcr reactions on a single sample | |
| CN108624652B (zh) | 多重焦磷酸化激活性聚合反应在一个反应内扩增单位点多个序列几乎完全相同的模板 | |
| JP4601830B2 (ja) | カップル性ポリメラーゼ連鎖反応−制限エンドヌクレアーゼ消化−リガーゼ検出反応法 | |
| JP2003508074A5 (https=) | ||
| JP2009219445A (ja) | Ｖｋｏｒｃ１遺伝子上の１塩基多型を検出する方法およびプライマーセット |