JP2010519896A5 - - Google Patents
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- Publication number
- JP2010519896A5 JP2010519896A5 JP2009551268A JP2009551268A JP2010519896A5 JP 2010519896 A5 JP2010519896 A5 JP 2010519896A5 JP 2009551268 A JP2009551268 A JP 2009551268A JP 2009551268 A JP2009551268 A JP 2009551268A JP 2010519896 A5 JP2010519896 A5 JP 2010519896A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleic acid
- target nucleic
- nucleotide
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013615 primer Substances 0.000 claims 74
- 150000007523 nucleic acids Chemical class 0.000 claims 45
- 108020004707 nucleic acids Proteins 0.000 claims 36
- 102000039446 nucleic acids Human genes 0.000 claims 36
- 125000003729 nucleotide group Chemical group 0.000 claims 33
- 239000002773 nucleotide Substances 0.000 claims 32
- 230000000295 complement effect Effects 0.000 claims 28
- 238000000034 method Methods 0.000 claims 19
- 108091034117 Oligonucleotide Proteins 0.000 claims 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 9
- 230000015556 catabolic process Effects 0.000 claims 7
- 238000006731 degradation reaction Methods 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 5
- 230000002452 interceptive effect Effects 0.000 claims 5
- 239000000203 mixture Substances 0.000 claims 5
- 239000002777 nucleoside Substances 0.000 claims 4
- -1 nucleoside triphosphate Chemical class 0.000 claims 4
- 235000011178 triphosphate Nutrition 0.000 claims 4
- 239000001226 triphosphate Substances 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 3
- 238000000137 annealing Methods 0.000 claims 3
- 238000009396 hybridization Methods 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 108010037497 3'-nucleotidase Proteins 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 238000005336 cracking Methods 0.000 claims 2
- 238000000354 decomposition reaction Methods 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- JCAQMQLAHNGVPY-UUOKFMHZSA-N [(2r,3s,4r,5r)-3,4-dihydroxy-5-(2,2,4-trioxo-1h-imidazo[4,5-c][1,2,6]thiadiazin-7-yl)oxolan-2-yl]methyl dihydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(NS(=O)(=O)NC2=O)=C2N=C1 JCAQMQLAHNGVPY-UUOKFMHZSA-N 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0703997.7 | 2007-03-01 | ||
| GBGB0703997.7A GB0703997D0 (en) | 2007-03-01 | 2007-03-01 | Methods for detecting nucleic sequences |
| PCT/GB2008/000699 WO2008104791A1 (en) | 2007-03-01 | 2008-02-29 | Methods for detecting nucleic acid sequences |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010519896A JP2010519896A (ja) | 2010-06-10 |
| JP2010519896A5 true JP2010519896A5 (https=) | 2011-04-21 |
| JP5235905B2 JP5235905B2 (ja) | 2013-07-10 |
Family
ID=37965756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009551268A Expired - Fee Related JP5235905B2 (ja) | 2007-03-01 | 2008-02-29 | 核酸配列の検出方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8440405B2 (https=) |
| EP (1) | EP2129802B1 (https=) |
| JP (1) | JP5235905B2 (https=) |
| CN (1) | CN101680030B (https=) |
| GB (1) | GB0703997D0 (https=) |
| WO (1) | WO2008104791A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0703996D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Nucleic acid detection |
| GB0909333D0 (en) * | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| US20100285537A1 (en) * | 2009-04-02 | 2010-11-11 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| JP5822843B2 (ja) * | 2009-12-21 | 2015-11-24 | シージーン アイエヌシー | Ｔｓｇプライマーターゲット検出 |
| DE102010003781B4 (de) | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
| CN101871007B (zh) * | 2010-05-07 | 2014-04-30 | 无锡锐奇基因生物科技有限公司 | 用标记探针进行检测和熔解曲线分析的方法 |
| GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| US9255293B2 (en) * | 2010-11-01 | 2016-02-09 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| WO2012174289A2 (en) * | 2011-06-14 | 2012-12-20 | University Of Southern California | A novel fluorescence-based assay for the rapid detection and quantification of deoxyribonucleoside triphosphates |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| US20140377762A1 (en) * | 2011-12-19 | 2014-12-25 | 360 Genomics Ltd. | Method for enriching and detection of variant target nucleic acids |
| JP6200948B2 (ja) | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| EP3022323B1 (en) * | 2013-07-18 | 2019-04-24 | President and Fellows of Harvard College | Specific nucleic acid amplification with compounded selectivity |
| GB201312995D0 (en) * | 2013-07-19 | 2013-09-04 | Atlas Genetics Ltd | Methods and kits for specific nucleic acid amplification and detection |
| CN104561248A (zh) * | 2013-10-22 | 2015-04-29 | 常州金麦格生物技术有限公司 | 用于检测靶核酸的引物和其应用 |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| EP3313573A2 (en) | 2015-07-22 | 2018-05-02 | The University of North Carolina at Chapel Hill | Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods |
| ES2893411T3 (es) * | 2016-09-15 | 2022-02-09 | Hoffmann La Roche | Procedimientos para realizar PCR multiplexada |
| WO2020068174A2 (en) | 2018-05-18 | 2020-04-02 | The University Of North Carolina At Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| WO2023125898A1 (zh) * | 2021-12-31 | 2023-07-06 | 柏锘（上海）医疗科技有限公司 | 核酸检测方法与系统 |
| CN114350774B (zh) * | 2022-01-28 | 2024-07-09 | 赛纳生物科技(北京)有限公司 | 一种芯片表面固相引物剪切效率的检测方法 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2650840B1 (fr) | 1989-08-11 | 1991-11-29 | Bertin & Cie | Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| GB9211979D0 (en) * | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| DE69432919T2 (de) * | 1993-12-28 | 2004-05-27 | Tanabe Seiyaku Co., Ltd. | Verfahren für den Nachweis spezifischer Polynukleotiden |
| JP4540754B2 (ja) * | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| DE19782089T1 (de) * | 1996-10-29 | 1999-12-23 | Univ Nebraska At Lincoln Linco | Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang |
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| HK1048339A1 (zh) * | 1999-03-18 | 2003-03-28 | 埃克西库恩公司 | 利用特异性lna引物检测基因突变 |
| IL149676A0 (en) * | 1999-12-02 | 2002-11-10 | Dna Sciences Inc | Methods and kits for determining nucleotide variations |
| JP3638516B2 (ja) * | 2000-09-28 | 2005-04-13 | 株式会社日立製作所 | 核酸検出方法および核酸検出キット |
| US6391592B1 (en) * | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| CN101074450A (zh) | 2001-09-24 | 2007-11-21 | 一兰姆达公司 | 诊断探针检测系统 |
| DE60323611D1 (de) * | 2002-03-01 | 2008-10-30 | Integrated Dna Tech Inc | Polynomiale amplifikation von nukleinsäuren |
| US20030211483A1 (en) | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| WO2004003173A2 (en) * | 2002-07-01 | 2004-01-08 | Cleveland State University | Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides |
| JP4116856B2 (ja) * | 2002-10-02 | 2008-07-09 | 富士フイルム株式会社 | １塩基多型の検出方法 |
| US20040121374A1 (en) | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| GB2395556A (en) | 2002-11-22 | 2004-05-26 | Dynal Biotech Ltd | Nucleic acid probe |
| WO2004065628A1 (en) * | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| US20060183136A1 (en) * | 2004-11-05 | 2006-08-17 | Genevieve Pont-Kingdon | Method for haplotyping and genotyping by melting curve analysis of hybridization probes |
| WO2007008997A2 (en) | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
| WO2007045890A1 (en) | 2005-10-21 | 2007-04-26 | Primerdesign Ltd | Probe |
| WO2007106534A2 (en) | 2006-03-14 | 2007-09-20 | Harbor-Ucla Research And Education Institute | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
-
2007
- 2007-03-01 GB GBGB0703997.7A patent/GB0703997D0/en not_active Ceased
-
2008
- 2008-02-29 US US12/529,203 patent/US8440405B2/en not_active Expired - Fee Related
- 2008-02-29 EP EP08750469.2A patent/EP2129802B1/en not_active Not-in-force
- 2008-02-29 JP JP2009551268A patent/JP5235905B2/ja not_active Expired - Fee Related
- 2008-02-29 CN CN200880012889.8A patent/CN101680030B/zh not_active Expired - Fee Related
- 2008-02-29 WO PCT/GB2008/000699 patent/WO2008104791A1/en not_active Ceased
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