JP2012520080A5 - - Google Patents
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- Publication number
- JP2012520080A5 JP2012520080A5 JP2011554206A JP2011554206A JP2012520080A5 JP 2012520080 A5 JP2012520080 A5 JP 2012520080A5 JP 2011554206 A JP2011554206 A JP 2011554206A JP 2011554206 A JP2011554206 A JP 2011554206A JP 2012520080 A5 JP2012520080 A5 JP 2012520080A5
- Authority
- JP
- Japan
- Prior art keywords
- reaction mixture
- amplification reaction
- double
- additive
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20256509P | 2009-03-12 | 2009-03-12 | |
| US61/202,565 | 2009-03-12 | ||
| PCT/US2010/027011 WO2010105074A1 (en) | 2009-03-12 | 2010-03-11 | Reagents and methods for pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012520080A JP2012520080A (ja) | 2012-09-06 |
| JP2012520080A5 true JP2012520080A5 (https=) | 2013-05-02 |
| JP5757885B2 JP5757885B2 (ja) | 2015-08-05 |
Family
ID=42244601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011554206A Expired - Fee Related JP5757885B2 (ja) | 2009-03-12 | 2010-03-11 | Ｐｃｒ用の試薬および方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US9034605B2 (https=) |
| EP (2) | EP2406394B1 (https=) |
| JP (1) | JP5757885B2 (https=) |
| CN (1) | CN102421918B (https=) |
| AU (1) | AU2010224100B2 (https=) |
| CA (1) | CA2755207A1 (https=) |
| WO (1) | WO2010105074A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008105814A2 (en) | 2006-08-22 | 2008-09-04 | Los Alamos National Security, Llc | Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
| EP3067694A1 (en) | 2008-05-05 | 2016-09-14 | Los Alamos National Security, LLC | Lateral flow-based nucleic acid sample preparation device, integrated with passive fluid flow control |
| US9034605B2 (en) | 2009-03-12 | 2015-05-19 | Brandeis University | Reagents and methods for PCR |
| WO2012075231A1 (en) * | 2010-12-03 | 2012-06-07 | Brandeis University | Methods and kits for detecting nucleic acid mutants in wild-type populations |
| US10519492B2 (en) | 2011-04-20 | 2019-12-31 | Mesa Biotech, Inc. | Integrated device for nucleic acid detection and identification |
| CN104204228A (zh) | 2012-02-14 | 2014-12-10 | 康奈尔大学 | 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法 |
| ES2730675T3 (es) | 2013-01-23 | 2019-11-12 | Univ Brandeis | Reactivos para mejorar la precisión de la PCR |
| KR102126031B1 (ko) * | 2013-07-23 | 2020-07-08 | 삼성전자주식회사 | 유전적 변이를 함유하는 핵산 검출 방법 |
| CN105378160B (zh) * | 2013-08-12 | 2018-04-17 | 伯乐生命医学产品有限公司 | 具有碱基配对的寡聚体的扩增报告子 |
| WO2015038869A1 (en) * | 2013-09-13 | 2015-03-19 | Brandeis University | Modified polymerase compositions, methods and kits |
| US10240178B2 (en) | 2014-12-19 | 2019-03-26 | Brandeis University | Mispriming prevention reagents |
| US20160310948A1 (en) | 2015-04-24 | 2016-10-27 | Mesa Biotech, Inc. | Fluidic Test Cassette |
| EP3332024B1 (en) * | 2015-08-06 | 2020-10-28 | F.Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| WO2017120531A1 (en) | 2016-01-08 | 2017-07-13 | Bio-Rad Laboratories, Inc. | Multiple beads per droplet resolution |
| EP4219519A3 (en) * | 2016-04-01 | 2023-09-13 | Chromacode, Inc. | Competitive probes for engineering signal generation |
| CN107541508A (zh) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒 |
| CN108774639B (zh) * | 2018-05-31 | 2023-05-30 | 澳門帝傑數碼基因有限公司 | 一种定向聚合的荧光探针pcr |
| EP3818170A4 (en) * | 2018-07-02 | 2022-03-09 | Swift Biosciences, Inc. | Temperature controlled dna polymerase inhibitors |
| US20220307066A1 (en) * | 2019-06-14 | 2022-09-29 | University Of Washington | An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy |
| WO2020264067A1 (en) * | 2019-06-25 | 2020-12-30 | Bio-Rad Laboratories, Inc | Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase |
| EP4107264A4 (en) * | 2020-02-21 | 2024-06-05 | Mission Bio, Inc. | Enhanced detection of target nucleic acids by removal of dna-rna cross contamination |
| JP7209980B2 (ja) | 2020-12-11 | 2023-01-23 | 東洋紡株式会社 | Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体 |
| EP4367235A1 (en) * | 2021-07-06 | 2024-05-15 | Singular Genomics Systems, Inc. | Compositions and methods for detecting genetic features |
| CN115747328A (zh) * | 2022-08-19 | 2023-03-07 | 福建师范大学 | 一种提高单链dna产量和改善产物比例的方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5066584A (en) | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US20050242506A1 (en) | 1994-07-22 | 2005-11-03 | Shuffle Master, Inc. | Poker game variation with variable size wagers and play against a pay table |
| DE69638318D1 (de) | 1995-06-07 | 2011-02-17 | Gilead Sciences Inc | Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| EP0892808B1 (en) | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
| US6277607B1 (en) | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| US7198897B2 (en) | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| WO2004090153A2 (en) * | 2003-04-01 | 2004-10-21 | Eragen Biosciences,Inc. | Polymerase inhibitor and method of using same |
| KR20130082179A (ko) * | 2004-02-18 | 2013-07-18 | 크로모셀 코포레이션 | 신호 프로브를 이용하는 방법 및 물질 |
| EP1802774B1 (en) * | 2004-10-18 | 2012-01-11 | Brandeis University | Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification |
| WO2006044994A2 (en) * | 2004-10-18 | 2006-04-27 | Brandeis University | Primers, probes and methods for nucleic acid amplification |
| CA2614444C (en) | 2005-07-07 | 2015-12-08 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
| DE602006008150D1 (de) * | 2006-10-12 | 2009-09-10 | Bio Rad Pasteur | Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren |
| AU2007334502B2 (en) * | 2006-12-14 | 2011-12-15 | Novartis Ag | Compositions and methods to treat muscular & cardiovascular disorders |
| US9034605B2 (en) | 2009-03-12 | 2015-05-19 | Brandeis University | Reagents and methods for PCR |
-
2010
- 2010-03-11 US US13/256,038 patent/US9034605B2/en active Active
- 2010-03-11 WO PCT/US2010/027011 patent/WO2010105074A1/en not_active Ceased
- 2010-03-11 EP EP10708682.9A patent/EP2406394B1/en not_active Not-in-force
- 2010-03-11 CA CA2755207A patent/CA2755207A1/en not_active Abandoned
- 2010-03-11 AU AU2010224100A patent/AU2010224100B2/en not_active Ceased
- 2010-03-11 CN CN201080020170.6A patent/CN102421918B/zh not_active Expired - Fee Related
- 2010-03-11 JP JP2011554206A patent/JP5757885B2/ja not_active Expired - Fee Related
- 2010-03-11 EP EP13195156.8A patent/EP2703501A1/en not_active Withdrawn
-
2015
- 2015-05-18 US US14/715,299 patent/US9758813B2/en active Active
-
2017
- 2017-09-08 US US15/698,992 patent/US20180002739A1/en not_active Abandoned
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