CA2755207A1 - Reagents and methods for pcr - Google Patents

Reagents and methods for pcr Download PDF

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Publication number
CA2755207A1
CA2755207A1 CA2755207A CA2755207A CA2755207A1 CA 2755207 A1 CA2755207 A1 CA 2755207A1 CA 2755207 A CA2755207 A CA 2755207A CA 2755207 A CA2755207 A CA 2755207A CA 2755207 A1 CA2755207 A1 CA 2755207A1
Authority
CA
Canada
Prior art keywords
additive
primer
amplification
stranded
double
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2755207A
Other languages
English (en)
French (fr)
Inventor
Lawrence J. Wangh
John Rice
Nicholas Rice
Yanwei Jia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of CA2755207A1 publication Critical patent/CA2755207A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2755207A 2009-03-12 2010-03-11 Reagents and methods for pcr Abandoned CA2755207A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20256509P 2009-03-12 2009-03-12
US61/202,565 2009-03-12
PCT/US2010/027011 WO2010105074A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Publications (1)

Publication Number Publication Date
CA2755207A1 true CA2755207A1 (en) 2010-09-16

Family

ID=42244601

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2755207A Abandoned CA2755207A1 (en) 2009-03-12 2010-03-11 Reagents and methods for pcr

Country Status (7)

Country Link
US (3) US9034605B2 (https=)
EP (2) EP2406394B1 (https=)
JP (1) JP5757885B2 (https=)
CN (1) CN102421918B (https=)
AU (1) AU2010224100B2 (https=)
CA (1) CA2755207A1 (https=)
WO (1) WO2010105074A1 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
EP3067694A1 (en) 2008-05-05 2016-09-14 Los Alamos National Security, LLC Lateral flow-based nucleic acid sample preparation device, integrated with passive fluid flow control
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
US10519492B2 (en) 2011-04-20 2019-12-31 Mesa Biotech, Inc. Integrated device for nucleic acid detection and identification
CN104204228A (zh) 2012-02-14 2014-12-10 康奈尔大学 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法
ES2730675T3 (es) 2013-01-23 2019-11-12 Univ Brandeis Reactivos para mejorar la precisión de la PCR
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
US10240178B2 (en) 2014-12-19 2019-03-26 Brandeis University Mispriming prevention reagents
US20160310948A1 (en) 2015-04-24 2016-10-27 Mesa Biotech, Inc. Fluidic Test Cassette
EP3332024B1 (en) * 2015-08-06 2020-10-28 F.Hoffmann-La Roche Ag Target enrichment by single probe primer extension
WO2017120531A1 (en) 2016-01-08 2017-07-13 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EP4219519A3 (en) * 2016-04-01 2023-09-13 Chromacode, Inc. Competitive probes for engineering signal generation
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
CN108774639B (zh) * 2018-05-31 2023-05-30 澳門帝傑數碼基因有限公司 一种定向聚合的荧光探针pcr
EP3818170A4 (en) * 2018-07-02 2022-03-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
US20220307066A1 (en) * 2019-06-14 2022-09-29 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy
WO2020264067A1 (en) * 2019-06-25 2020-12-30 Bio-Rad Laboratories, Inc Compositions and methods for enhancing reverse transcriptase activity and/or reducing the inhibition of reverse transcriptase
EP4107264A4 (en) * 2020-02-21 2024-06-05 Mission Bio, Inc. Enhanced detection of target nucleic acids by removal of dna-rna cross contamination
JP7209980B2 (ja) 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
EP4367235A1 (en) * 2021-07-06 2024-05-15 Singular Genomics Systems, Inc. Compositions and methods for detecting genetic features
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US20050242506A1 (en) 1994-07-22 2005-11-03 Shuffle Master, Inc. Poker game variation with variable size wagers and play against a pay table
DE69638318D1 (de) 1995-06-07 2011-02-17 Gilead Sciences Inc Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
KR20130082179A (ko) * 2004-02-18 2013-07-18 크로모셀 코포레이션 신호 프로브를 이용하는 방법 및 물질
EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
CA2614444C (en) 2005-07-07 2015-12-08 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
DE602006008150D1 (de) * 2006-10-12 2009-09-10 Bio Rad Pasteur Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren
AU2007334502B2 (en) * 2006-12-14 2011-12-15 Novartis Ag Compositions and methods to treat muscular & cardiovascular disorders
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR

Also Published As

Publication number Publication date
AU2010224100B2 (en) 2015-10-22
US9758813B2 (en) 2017-09-12
JP5757885B2 (ja) 2015-08-05
AU2010224100A1 (en) 2011-10-27
JP2012520080A (ja) 2012-09-06
US20120088275A1 (en) 2012-04-12
EP2406394B1 (en) 2014-01-08
US9034605B2 (en) 2015-05-19
WO2010105074A1 (en) 2010-09-16
EP2703501A1 (en) 2014-03-05
CN102421918A (zh) 2012-04-18
US20180002739A1 (en) 2018-01-04
CN102421918B (zh) 2016-01-20
EP2406394A1 (en) 2012-01-18
US20150322503A1 (en) 2015-11-12

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150311

FZDE Dead

Effective date: 20170313