JP2010535480A5 - - Google Patents
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- Publication number
- JP2010535480A5 JP2010535480A5 JP2010519376A JP2010519376A JP2010535480A5 JP 2010535480 A5 JP2010535480 A5 JP 2010535480A5 JP 2010519376 A JP2010519376 A JP 2010519376A JP 2010519376 A JP2010519376 A JP 2010519376A JP 2010535480 A5 JP2010535480 A5 JP 2010535480A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- target sequence
- blocker oligonucleotide
- oligonucleotide
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 24
- 239000002773 nucleotide Substances 0.000 claims 14
- 125000003729 nucleotide group Chemical group 0.000 claims 14
- 238000000034 method Methods 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims 7
- 108091033319 polynucleotide Proteins 0.000 claims 7
- 239000002157 polynucleotide Substances 0.000 claims 7
- 102000040430 polynucleotide Human genes 0.000 claims 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 238000009396 hybridization Methods 0.000 claims 4
- 238000002844 melting Methods 0.000 claims 4
- 230000008018 melting Effects 0.000 claims 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 claims 4
- 108060002716 Exonuclease Proteins 0.000 claims 3
- 102000013165 exonuclease Human genes 0.000 claims 3
- 239000000523 sample Substances 0.000 claims 3
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 claims 2
- 239000007997 Tricine buffer Substances 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 claims 2
- 238000009830 intercalation Methods 0.000 claims 2
- 235000011056 potassium acetate Nutrition 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 101710163270 Nuclease Proteins 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US96408907P | 2007-08-08 | 2007-08-08 | |
| US60/964,089 | 2007-08-08 | ||
| PCT/EP2008/006476 WO2009019008A1 (en) | 2007-08-08 | 2008-08-07 | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010535480A JP2010535480A (ja) | 2010-11-25 |
| JP2010535480A5 true JP2010535480A5 (https=) | 2011-06-23 |
| JP5524838B2 JP5524838B2 (ja) | 2014-06-18 |
Family
ID=39942715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010519376A Expired - Fee Related JP5524838B2 (ja) | 2007-08-08 | 2008-08-07 | オリゴヌクレオチド、及び５’−３’エキソヌクレアーゼ活性を著しく欠如するポリメラーゼを使用する、増幅の抑制 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8071338B2 (https=) |
| EP (1) | EP2173910B1 (https=) |
| JP (1) | JP5524838B2 (https=) |
| CN (1) | CN101778952B (https=) |
| CA (1) | CA2693384C (https=) |
| ES (1) | ES2562789T3 (https=) |
| WO (1) | WO2009019008A1 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2183379B1 (en) | 2007-08-01 | 2015-04-15 | Dana Farber Cancer Institute | Enrichment of a target sequence |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| CA2778532A1 (en) | 2009-10-27 | 2011-05-12 | Swift Biosciences, Inc. | Polynucleotide primers and probes |
| CN102859003B (zh) | 2010-03-08 | 2014-07-30 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
| US9944979B2 (en) * | 2010-09-10 | 2018-04-17 | Lsi Medience Corporation | Inhibition method of nucleic acid amplification by photoirradiation and method of selective nucleic acid amplification with high sensitivity |
| AU2011319755B2 (en) | 2010-10-27 | 2017-02-23 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| AU2012223438A1 (en) * | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
| EP3333269B1 (en) * | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Methods to enable multiplex cold-pcr |
| US9796748B2 (en) | 2011-05-02 | 2017-10-24 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| EP2705162B1 (en) * | 2011-05-04 | 2018-07-11 | Biocept, Inc. | Methods for detecting nucleic acid sequence variants |
| WO2012162621A1 (en) * | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
| EP2758458A4 (en) | 2011-07-10 | 2015-10-21 | Harvard College | COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS |
| EP2570487A1 (en) | 2011-09-16 | 2013-03-20 | Lexogen GmbH | Nucleic acid transcription method |
| DK2756098T3 (en) | 2011-09-16 | 2018-09-03 | Lexogen Gmbh | Process for Preparing a Library of Nucleic Acid Molecules |
| US9260714B2 (en) * | 2011-12-02 | 2016-02-16 | Roche Molecular Systems, Inc. | Suppression of non-specific amplification with high-homology oligonucleotides |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| WO2014177540A1 (en) * | 2013-04-29 | 2014-11-06 | Qiagen Marseille | A method for dna amplification with a blocking oligonucleotide |
| AU2014336987A1 (en) * | 2013-10-19 | 2016-04-14 | Trovagene, Inc. | Detecting mutations in disease over time |
| JP2017515484A (ja) * | 2014-05-19 | 2017-06-15 | ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| US12391985B2 (en) | 2014-08-07 | 2025-08-19 | Pharmassist Ltd | Method of determining PIK3CA mutational status in a sample |
| RS59710B1 (sr) * | 2014-08-07 | 2020-01-31 | Pharmassist Ltd | Metod određivanja mutacionog statusa pik3ca u uzorku |
| EP4063516A1 (en) * | 2014-09-26 | 2022-09-28 | Janssen Pharmaceutica NV | Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor |
| US9909169B2 (en) | 2014-12-17 | 2018-03-06 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression |
| JP5813263B1 (ja) * | 2015-03-31 | 2015-11-17 | 日鉄住金環境株式会社 | 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド |
| US9657353B2 (en) | 2015-06-26 | 2017-05-23 | Great Basin Scientific, Inc. | Specific detection of organisms derived from a sample |
| US11414686B2 (en) | 2016-05-06 | 2022-08-16 | William Marsh Rice University | Stoichiometric nucleic acid purification using randomer capture probe libraries |
| EP3464596B1 (en) * | 2016-06-07 | 2025-07-30 | Cepheid | Thermostable polymerase inhibitor compositions and methods |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
| WO2019099420A1 (en) * | 2017-11-15 | 2019-05-23 | Yan Wang | A method for detecting multiple dna mutations and copy number variations |
| US12331350B2 (en) | 2018-02-20 | 2025-06-17 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| ES2874753T3 (es) | 2018-07-19 | 2021-11-05 | Biofidelity Ltd | Método mejorado de detección de secuencia de polinucleótidos |
| CN112654714B (zh) * | 2018-12-17 | 2024-10-11 | 伊卢米纳剑桥有限公司 | 用于测序的引物寡核苷酸 |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| WO2023039228A2 (en) * | 2021-09-13 | 2023-03-16 | Nexgen Cancer Detection Llc | Methods for detecting a target polynucleotide in a sample comprising a mixture of the target polynucleotide and a non-target polynucleotide |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2163393C (en) | 1994-11-30 | 2003-04-22 | Colleen Marie Nycz | Amplification and detection of mycobacteria nucleic acids |
| US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| CA2356697C (en) * | 1999-01-06 | 2010-06-22 | Cornell Research Foundation, Inc. | Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing |
| EP1253205A1 (en) | 2001-04-24 | 2002-10-30 | LION Bioscience AG | Method of blocking amplification of selected sequences |
| GB0406863D0 (en) | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
| EP1794173B1 (en) | 2004-09-10 | 2010-08-04 | Human Genetic Signatures PTY Ltd | Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn) |
-
2008
- 2008-08-05 US US12/186,311 patent/US8071338B2/en not_active Expired - Fee Related
- 2008-08-07 EP EP08785392.5A patent/EP2173910B1/en not_active Not-in-force
- 2008-08-07 CA CA2693384A patent/CA2693384C/en not_active Expired - Fee Related
- 2008-08-07 ES ES08785392.5T patent/ES2562789T3/es active Active
- 2008-08-07 JP JP2010519376A patent/JP5524838B2/ja not_active Expired - Fee Related
- 2008-08-07 WO PCT/EP2008/006476 patent/WO2009019008A1/en not_active Ceased
- 2008-08-07 CN CN200880102467.XA patent/CN101778952B/zh not_active Expired - Fee Related
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