ES2562789T3 - Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' - Google Patents

Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' Download PDF

Info

Publication number
ES2562789T3
ES2562789T3 ES08785392.5T ES08785392T ES2562789T3 ES 2562789 T3 ES2562789 T3 ES 2562789T3 ES 08785392 T ES08785392 T ES 08785392T ES 2562789 T3 ES2562789 T3 ES 2562789T3
Authority
ES
Spain
Prior art keywords
sequence
blocking oligonucleotide
oligonucleotide
target
target sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08785392.5T
Other languages
English (en)
Spanish (es)
Inventor
Nicolas Newton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2562789T3 publication Critical patent/ES2562789T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
ES08785392.5T 2007-08-08 2008-08-07 Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3' Active ES2562789T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96408907P 2007-08-08 2007-08-08
US964089P 2007-08-08
PCT/EP2008/006476 WO2009019008A1 (en) 2007-08-08 2008-08-07 Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Publications (1)

Publication Number Publication Date
ES2562789T3 true ES2562789T3 (es) 2016-03-08

Family

ID=39942715

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08785392.5T Active ES2562789T3 (es) 2007-08-08 2008-08-07 Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3'

Country Status (7)

Country Link
US (1) US8071338B2 (https=)
EP (1) EP2173910B1 (https=)
JP (1) JP5524838B2 (https=)
CN (1) CN101778952B (https=)
CA (1) CA2693384C (https=)
ES (1) ES2562789T3 (https=)
WO (1) WO2009019008A1 (https=)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2183379B1 (en) 2007-08-01 2015-04-15 Dana Farber Cancer Institute Enrichment of a target sequence
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
CA2778532A1 (en) 2009-10-27 2011-05-12 Swift Biosciences, Inc. Polynucleotide primers and probes
CN102859003B (zh) 2010-03-08 2014-07-30 达纳-法伯癌症研究院有限公司 使用参考封闭序列的全cold-pcr富集
US9944979B2 (en) * 2010-09-10 2018-04-17 Lsi Medience Corporation Inhibition method of nucleic acid amplification by photoirradiation and method of selective nucleic acid amplification with high sensitivity
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
AU2012223438A1 (en) * 2011-02-28 2013-09-26 Transgenomic, Inc Kit and method for sequencing a target DNA in a mixed population
EP3333269B1 (en) * 2011-03-31 2021-05-05 Dana-Farber Cancer Institute, Inc. Methods to enable multiplex cold-pcr
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
EP2705162B1 (en) * 2011-05-04 2018-07-11 Biocept, Inc. Methods for detecting nucleic acid sequence variants
WO2012162621A1 (en) * 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
EP2758458A4 (en) 2011-07-10 2015-10-21 Harvard College COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
DK2756098T3 (en) 2011-09-16 2018-09-03 Lexogen Gmbh Process for Preparing a Library of Nucleic Acid Molecules
US9260714B2 (en) * 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide
AU2014336987A1 (en) * 2013-10-19 2016-04-14 Trovagene, Inc. Detecting mutations in disease over time
JP2017515484A (ja) * 2014-05-19 2017-06-15 ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
US12391985B2 (en) 2014-08-07 2025-08-19 Pharmassist Ltd Method of determining PIK3CA mutational status in a sample
RS59710B1 (sr) * 2014-08-07 2020-01-31 Pharmassist Ltd Metod određivanja mutacionog statusa pik3ca u uzorku
EP4063516A1 (en) * 2014-09-26 2022-09-28 Janssen Pharmaceutica NV Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
US11414686B2 (en) 2016-05-06 2022-08-16 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
EP3464596B1 (en) * 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019099420A1 (en) * 2017-11-15 2019-05-23 Yan Wang A method for detecting multiple dna mutations and copy number variations
US12331350B2 (en) 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
ES2874753T3 (es) 2018-07-19 2021-11-05 Biofidelity Ltd Método mejorado de detección de secuencia de polinucleótidos
CN112654714B (zh) * 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
WO2023039228A2 (en) * 2021-09-13 2023-03-16 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample comprising a mixture of the target polynucleotide and a non-target polynucleotide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163393C (en) 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
EP1794173B1 (en) 2004-09-10 2010-08-04 Human Genetic Signatures PTY Ltd Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn)

Also Published As

Publication number Publication date
EP2173910B1 (en) 2015-12-09
JP5524838B2 (ja) 2014-06-18
US8071338B2 (en) 2011-12-06
CA2693384A1 (en) 2009-02-12
US20090053720A1 (en) 2009-02-26
CN101778952A (zh) 2010-07-14
CN101778952B (zh) 2016-05-25
EP2173910A1 (en) 2010-04-14
CA2693384C (en) 2015-04-14
HK1146094A1 (zh) 2011-05-13
WO2009019008A1 (en) 2009-02-12
JP2010535480A (ja) 2010-11-25

Similar Documents

Publication Publication Date Title
ES2562789T3 (es) Supresión de la amplificación usando un oligonucleótido y una polimerasa que carece significativamente de actividad exonucleasa 5'-3'
ES2530273T3 (es) Amplificación de ácidos nucleicos específica de alelo
ES2608455T3 (es) Formato de sondas para detectar diferencias de ácidos nucleicos
ES2384681T3 (es) Extensión de cebadores correctora
ES2634428T3 (es) Amplificación de ácido nucleico con supresión específica de alelo de variantes de secuencia
EP1746172B1 (en) Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
ES2964624T3 (es) Detección de secuencias de ácidos nucleicos diana usando diferentes temperaturas de detección
ES2527653T3 (es) Amplificación específica de alelo usando un cebador con un nucleótido modificado
ES2414286T3 (es) Detección de ácidos nucleicos
CN101528945A (zh) 2’-终止子有关的焦磷酸解作用激活的聚合
AU2022294745B2 (en) Detection of multiple target nucleic acid using multiple detection temperatures
JP2015536685A (ja) 対立遺伝子特異的ｐｃｒにおける修飾されたリン酸及び修飾された塩基を有するプライマー
ES2656961T3 (es) Uso de G-Clamp para mejorar la PCR específica de alelo
ES2886605T3 (es) Procedimiento genérico para la estabilización de ARN específico
EP1606389B1 (en) Methods for polynucleotide sequence detection
US20160348158A1 (en) Format of Probes to Detect Nucleic Acid Differences
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity