JP2011500092A5 - - Google Patents
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- JP2011500092A5 JP2011500092A5 JP2010531293A JP2010531293A JP2011500092A5 JP 2011500092 A5 JP2011500092 A5 JP 2011500092A5 JP 2010531293 A JP2010531293 A JP 2010531293A JP 2010531293 A JP2010531293 A JP 2010531293A JP 2011500092 A5 JP2011500092 A5 JP 2011500092A5
- Authority
- JP
- Japan
- Prior art keywords
- population
- hybridizing
- oligonucleotides
- oligonucleotide
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims description 100
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 67
- 150000007523 nucleic acids Chemical class 0.000 claims description 49
- 238000000034 method Methods 0.000 claims description 47
- 108020004707 nucleic acids Proteins 0.000 claims description 47
- 102000039446 nucleic acids Human genes 0.000 claims description 47
- 239000013615 primer Substances 0.000 claims description 36
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 30
- 239000002773 nucleotide Substances 0.000 claims description 28
- 125000003729 nucleotide group Chemical group 0.000 claims description 24
- 239000003155 DNA primer Substances 0.000 claims description 21
- 239000002299 complementary DNA Substances 0.000 claims description 14
- 108020004418 ribosomal RNA Proteins 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000002194 synthesizing effect Effects 0.000 claims description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102100034343 Integrase Human genes 0.000 claims description 7
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 6
- 238000010804 cDNA synthesis Methods 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000012163 sequencing technique Methods 0.000 claims description 5
- 230000002438 mitochondrial effect Effects 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 claims description 3
- 230000037452 priming Effects 0.000 claims description 3
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 11
- 238000000137 annealing Methods 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US98308507P | 2007-10-26 | 2007-10-26 | |
| PCT/US2008/081206 WO2009055732A1 (en) | 2007-10-26 | 2008-10-24 | Cdna synthesis using non-random primers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011500092A JP2011500092A (ja) | 2011-01-06 |
| JP2011500092A5 true JP2011500092A5 (https=) | 2012-12-20 |
Family
ID=40253256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010531293A Pending JP2011500092A (ja) | 2007-10-26 | 2008-10-24 | 非ランダムプライマーを用いたｃＤＮＡ合成の方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US20100029511A1 (https=) |
| EP (1) | EP2209912A1 (https=) |
| JP (1) | JP2011500092A (https=) |
| CN (1) | CN102124126A (https=) |
| WO (1) | WO2009055732A1 (https=) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602006018352D1 (de) | 2005-12-06 | 2010-12-30 | Ambion Inc | Rückübertragungs-primer und verfahren zu deren entwurf |
| WO2009055732A1 (en) * | 2007-10-26 | 2009-04-30 | Rosetta Inpharmatics Llc | Cdna synthesis using non-random primers |
| AU2009205523A1 (en) * | 2008-01-14 | 2009-07-23 | Applied Biosystems, Llc | Compositions, methods, and kits for detecting ribonucleic acid |
| EP2475777A4 (en) * | 2009-09-11 | 2013-03-06 | Nugen Technologies Inc | COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS |
| WO2012064739A2 (en) * | 2010-11-08 | 2012-05-18 | The Trustees Of Columbia University In The City Of New York | Microbial enrichment primers |
| WO2012174214A1 (en) * | 2011-06-15 | 2012-12-20 | The Regents Of The University Of California | High resolution analysis of mammalian transcriptome using gene pool specific primers |
| WO2013059746A1 (en) | 2011-10-19 | 2013-04-25 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
| EP2807292B1 (en) | 2012-01-26 | 2019-05-22 | Tecan Genomics, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| US9777334B2 (en) | 2012-04-30 | 2017-10-03 | The Research Foundation for State University of New York | Cancer blood test using BC200 RNA isolated from peripheral blood for diagnosis and treatment of invasive breast cancer |
| CA2877094A1 (en) * | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| GB201301857D0 (en) * | 2013-02-01 | 2013-03-20 | Selvi Ozan | Method |
| US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
| WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| EP3105325B2 (en) * | 2014-02-13 | 2024-10-23 | Takara Bio USA, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| WO2015200893A2 (en) * | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US10711296B2 (en) * | 2015-03-24 | 2020-07-14 | Sigma-Aldrich Co. Llc | Directional amplification of RNA |
| CN105985949A (zh) * | 2015-11-02 | 2016-10-05 | 中国动物卫生与流行病学中心 | 一种rna高通量测序文库构建方法 |
| WO2017140659A1 (en) * | 2016-02-15 | 2017-08-24 | F. Hoffmann-La Roche Ag | System and method for targeted depletion of nucleic acids |
| US10472666B2 (en) | 2016-02-15 | 2019-11-12 | Roche Sequencing Solutions, Inc. | System and method for targeted depletion of nucleic acids |
| JP7019590B2 (ja) | 2016-03-31 | 2022-02-15 | バークレー ライツ，インコーポレイテッド | 核酸安定化試薬、キット、及びその使用方法 |
| CN111621548A (zh) * | 2016-04-26 | 2020-09-04 | 序康医疗科技（苏州）有限公司 | 扩增dna的方法 |
| GB201621477D0 (en) * | 2016-12-16 | 2017-02-01 | Multiplicom Nv | Modified multiplex and multistep amplification reactions and reagents therefor |
| WO2018165593A1 (en) * | 2017-03-09 | 2018-09-13 | iRepertoire, Inc. | Dimer avoided multiplex polymerase chain reaction for amplification of multiple targets |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CN112105745A (zh) * | 2018-05-07 | 2020-12-18 | 罗氏创新中心哥本哈根有限公司 | 用于寡核苷酸治疗剂的大规模平行发现方法 |
| WO2020124391A1 (zh) * | 2018-12-18 | 2020-06-25 | 深圳先进技术研究院 | 骨密度性状遗传力分析方法及装置 |
| US20220154180A1 (en) | 2019-03-13 | 2022-05-19 | Toyobo Co., Ltd. | Production and amplification of nucleic acids |
| CN111534512A (zh) * | 2019-09-11 | 2020-08-14 | 广东美格基因科技有限公司 | 一种去除核糖体rna的反转录引物池、试剂盒及去除核糖体rna的方法 |
| EP3851542A1 (en) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Depletion of abundant uninformative sequences |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
| US11280028B1 (en) | 2021-02-24 | 2022-03-22 | Agency For Science, Technology And Research (A*Star) | Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid |
| CN120584195A (zh) | 2023-01-26 | 2025-09-02 | 东洋纺株式会社 | 源自核糖体rna的逆转录的抑制 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| EP1007730A4 (en) * | 1996-08-30 | 2004-04-28 | Invitrogen Corp | METHODS OF IDENTIFYING AND ISOLATING SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA |
| EP1007739A2 (en) * | 1997-09-05 | 2000-06-14 | Sidney Kimmel Cancer Center | Selection of pcr primer pairs to amplify a group of nucleotide sequences |
| US6787308B2 (en) * | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| MXPA02012739A (es) * | 2001-03-09 | 2004-04-20 | Nugen Technologies Inc | Metodos y composiciones para amplificacion de secuencias de arn. |
| WO2003020979A1 (en) * | 2001-08-31 | 2003-03-13 | Rosetta Inpharmactis Llc. | Methods for preparing nucleic acid samples |
| CA2626977A1 (en) * | 2005-10-27 | 2007-05-03 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
| WO2009055732A1 (en) * | 2007-10-26 | 2009-04-30 | Rosetta Inpharmatics Llc | Cdna synthesis using non-random primers |
-
2008
- 2008-10-24 WO PCT/US2008/081206 patent/WO2009055732A1/en not_active Ceased
- 2008-10-24 JP JP2010531293A patent/JP2011500092A/ja active Pending
- 2008-10-24 EP EP08842031A patent/EP2209912A1/en not_active Withdrawn
- 2008-10-24 CN CN2008801228338A patent/CN102124126A/zh active Pending
-
2009
- 2009-07-24 US US12/509,312 patent/US20100029511A1/en not_active Abandoned
-
2010
- 2010-04-26 US US12/767,542 patent/US20110039732A1/en not_active Abandoned
-
2012
- 2012-12-10 US US13/710,285 patent/US20130252823A1/en not_active Abandoned
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