CN102124126A - 使用非随机引物的cdna合成 - Google Patents

使用非随机引物的cdna合成 Download PDF

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Publication number
CN102124126A
CN102124126A CN2008801228338A CN200880122833A CN102124126A CN 102124126 A CN102124126 A CN 102124126A CN 2008801228338 A CN2008801228338 A CN 2008801228338A CN 200880122833 A CN200880122833 A CN 200880122833A CN 102124126 A CN102124126 A CN 102124126A
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China
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population
oligonucleotides
nucleic acid
seq
rna
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CN2008801228338A
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Chinese (zh)
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C·K·雷蒙德
C·阿穆尔
J·卡斯尔
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Life Technologies Inc
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Life Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008801228338A 2007-10-26 2008-10-24 使用非随机引物的cdna合成 Pending CN102124126A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US98308507P 2007-10-26 2007-10-26
US60/983,085 2007-10-26
PCT/US2008/081206 WO2009055732A1 (en) 2007-10-26 2008-10-24 Cdna synthesis using non-random primers

Publications (1)

Publication Number Publication Date
CN102124126A true CN102124126A (zh) 2011-07-13

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Family Applications (1)

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CN2008801228338A Pending CN102124126A (zh) 2007-10-26 2008-10-24 使用非随机引物的cdna合成

Country Status (5)

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US (3) US20100029511A1 (https=)
EP (1) EP2209912A1 (https=)
JP (1) JP2011500092A (https=)
CN (1) CN102124126A (https=)
WO (1) WO2009055732A1 (https=)

Cited By (9)

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CN105985949A (zh) * 2015-11-02 2016-10-05 中国动物卫生与流行病学中心 一种rna高通量测序文库构建方法
WO2020124391A1 (zh) * 2018-12-18 2020-06-25 深圳先进技术研究院 骨密度性状遗传力分析方法及装置
CN112424376A (zh) * 2018-05-07 2021-02-26 罗氏创新中心哥本哈根有限公司 使用大规模平行测序对lna寡核苷酸治疗药物进行质量控制
CN113249435A (zh) * 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CN113265394A (zh) * 2014-02-13 2021-08-17 宝生物工程(美国) 有限公司 从核酸的初始集合中耗尽靶分子的方法、以及用于实践其的组合物和试剂盒
CN113278681A (zh) * 2020-01-20 2021-08-20 帝肯基因组学公司 大量非信息性序列的耗竭
US12037634B2 (en) 2012-08-14 2024-07-16 10X Genomics, Inc. Capsule array devices and methods of use
US12098423B2 (en) 2012-08-14 2024-09-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences

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DE602006018352D1 (de) 2005-12-06 2010-12-30 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
WO2009055732A1 (en) * 2007-10-26 2009-04-30 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers
AU2009205523A1 (en) * 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
EP2475777A4 (en) * 2009-09-11 2013-03-06 Nugen Technologies Inc COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS
WO2012064739A2 (en) * 2010-11-08 2012-05-18 The Trustees Of Columbia University In The City Of New York Microbial enrichment primers
WO2012174214A1 (en) * 2011-06-15 2012-12-20 The Regents Of The University Of California High resolution analysis of mammalian transcriptome using gene pool specific primers
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9777334B2 (en) 2012-04-30 2017-10-03 The Research Foundation for State University of New York Cancer blood test using BC200 RNA isolated from peripheral blood for diagnosis and treatment of invasive breast cancer
CA2877094A1 (en) * 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
GB201301857D0 (en) * 2013-02-01 2013-03-20 Selvi Ozan Method
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10711296B2 (en) * 2015-03-24 2020-07-14 Sigma-Aldrich Co. Llc Directional amplification of RNA
WO2017140659A1 (en) * 2016-02-15 2017-08-24 F. Hoffmann-La Roche Ag System and method for targeted depletion of nucleic acids
US10472666B2 (en) 2016-02-15 2019-11-12 Roche Sequencing Solutions, Inc. System and method for targeted depletion of nucleic acids
JP7019590B2 (ja) 2016-03-31 2022-02-15 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
CN111621548A (zh) * 2016-04-26 2020-09-04 序康医疗科技（苏州）有限公司 扩增dna的方法
GB201621477D0 (en) * 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
WO2018165593A1 (en) * 2017-03-09 2018-09-13 iRepertoire, Inc. Dimer avoided multiplex polymerase chain reaction for amplification of multiple targets
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US20220154180A1 (en) 2019-03-13 2022-05-19 Toyobo Co., Ltd. Production and amplification of nucleic acids
CN111534512A (zh) * 2019-09-11 2020-08-14 广东美格基因科技有限公司 一种去除核糖体rna的反转录引物池、试剂盒及去除核糖体rna的方法
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US11280028B1 (en) 2021-02-24 2022-03-22 Agency For Science, Technology And Research (A*Star) Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid
CN120584195A (zh) 2023-01-26 2025-09-02 东洋纺株式会社 源自核糖体rna的逆转录的抑制

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FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
EP1007730A4 (en) * 1996-08-30 2004-04-28 Invitrogen Corp METHODS OF IDENTIFYING AND ISOLATING SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA
EP1007739A2 (en) * 1997-09-05 2000-06-14 Sidney Kimmel Cancer Center Selection of pcr primer pairs to amplify a group of nucleotide sequences
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
MXPA02012739A (es) * 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
WO2003020979A1 (en) * 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
CA2626977A1 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
WO2009055732A1 (en) * 2007-10-26 2009-04-30 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12037634B2 (en) 2012-08-14 2024-07-16 10X Genomics, Inc. Capsule array devices and methods of use
US12098423B2 (en) 2012-08-14 2024-09-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN113265394A (zh) * 2014-02-13 2021-08-17 宝生物工程(美国) 有限公司 从核酸的初始集合中耗尽靶分子的方法、以及用于实践其的组合物和试剂盒
US11884963B2 (en) 2014-02-13 2024-01-30 Takara Bio Usa, Inc. Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same
US12497647B2 (en) 2014-02-13 2025-12-16 Takara Bio Usa, Inc. Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same
CN113249435A (zh) * 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CN113249435B (zh) * 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN105985949A (zh) * 2015-11-02 2016-10-05 中国动物卫生与流行病学中心 一种rna高通量测序文库构建方法
CN112424376A (zh) * 2018-05-07 2021-02-26 罗氏创新中心哥本哈根有限公司 使用大规模平行测序对lna寡核苷酸治疗药物进行质量控制
WO2020124391A1 (zh) * 2018-12-18 2020-06-25 深圳先进技术研究院 骨密度性状遗传力分析方法及装置
CN113278681A (zh) * 2020-01-20 2021-08-20 帝肯基因组学公司 大量非信息性序列的耗竭

Also Published As

Publication number Publication date
US20110039732A1 (en) 2011-02-17
US20100029511A1 (en) 2010-02-04
EP2209912A1 (en) 2010-07-28
WO2009055732A1 (en) 2009-04-30
JP2011500092A (ja) 2011-01-06
US20130252823A1 (en) 2013-09-26

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Application publication date: 20110713