AU2001241621B2 - Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination - Google Patents
Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination Download PDFInfo
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- AU2001241621B2 AU2001241621B2 AU2001241621A AU2001241621A AU2001241621B2 AU 2001241621 B2 AU2001241621 B2 AU 2001241621B2 AU 2001241621 A AU2001241621 A AU 2001241621A AU 2001241621 A AU2001241621 A AU 2001241621A AU 2001241621 B2 AU2001241621 B2 AU 2001241621B2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18431500P | 2000-02-23 | 2000-02-23 | |
| US60/184,315 | 2000-02-23 | ||
| US18703500P | 2000-03-06 | 2000-03-06 | |
| US60/187,035 | 2000-03-06 | ||
| US23718000P | 2000-10-03 | 2000-10-03 | |
| US60/237,180 | 2000-10-03 | ||
| PCT/US2001/005527 WO2001062975A2 (en) | 2000-02-23 | 2001-02-22 | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2001241621A1 AU2001241621A1 (en) | 2001-11-15 |
| AU2001241621B2 true AU2001241621B2 (en) | 2005-08-25 |
Family
ID=27391815
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001241621A Ceased AU2001241621B2 (en) | 2000-02-23 | 2001-02-22 | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination |
| AU4162101A Pending AU4162101A (en) | 2000-02-23 | 2001-02-22 | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU4162101A Pending AU4162101A (en) | 2000-02-23 | 2001-02-22 | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US6534269B2 (https=) |
| EP (2) | EP1259646B1 (https=) |
| JP (1) | JP4989004B2 (https=) |
| AT (1) | ATE441726T1 (https=) |
| AU (2) | AU2001241621B2 (https=) |
| CA (1) | CA2395391C (https=) |
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| WO (1) | WO2001062975A2 (https=) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7033763B2 (en) * | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| AUPR050700A0 (en) | 2000-10-03 | 2000-10-26 | Id+Plus Ltd | Detection method |
| AU2001291513B2 (en) * | 2000-10-03 | 2007-06-07 | Id+Plus Ltd | Nucleotide detection method |
| AU2002227156A1 (en) * | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| WO2002079516A1 (en) * | 2001-03-30 | 2002-10-10 | Applera Corporation | Nucleic acid analysis using non-templated nucleotide addition |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| EP1415004A4 (en) * | 2001-07-03 | 2005-01-19 | Univ Leland Stanford Junior | BIOLUMINESCENCE REGENERATING CIRCULATION (BRC) FOR NUCLEIC ACID QUANTIFICATION |
| US7668697B2 (en) * | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
| EP1509619B1 (en) * | 2002-05-10 | 2012-04-04 | City of Hope | Pyrophosphorolysis activated polymerization (pap) |
| WO2005086679A2 (en) * | 2004-03-05 | 2005-09-22 | Medical College Of Ohio | Methods and compositions for assessing nucleic acids and alleles |
| WO2005106036A2 (en) | 2004-04-12 | 2005-11-10 | Medical College Of Ohio | Methods and compositions for assaying analytes |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US20060060977A1 (en) * | 2004-09-22 | 2006-03-23 | Kabushiki Kaisha Toshiba | Semiconductor device |
| US7378260B2 (en) * | 2005-04-01 | 2008-05-27 | Applera Corporation | Products and methods for reducing dye artifacts |
| WO2009068268A1 (en) * | 2007-11-28 | 2009-06-04 | Roche Diagnostics Gmbh | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
| EP2644708B1 (en) | 2008-04-30 | 2014-12-17 | Integrated Dna Technologies, Inc. | RNASE-H-based assays utilizing modified RNA monomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CN101381766B (zh) * | 2008-06-11 | 2012-04-18 | 厦门大学 | 一种基因稀有突变的定量检测方法 |
| US8481685B2 (en) * | 2008-11-03 | 2013-07-09 | Kapa Biosystems | Modified DNA polymerases |
| US20100112565A1 (en) * | 2008-11-03 | 2010-05-06 | Life Technologies Corporation | Methods, kits, and reaction mixtures for high resolution melt genotyping |
| US8932813B2 (en) | 2010-12-13 | 2015-01-13 | Life Technologies Corporation | Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions |
| WO2012135053A2 (en) | 2011-03-25 | 2012-10-04 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| US10270033B2 (en) | 2015-10-26 | 2019-04-23 | Oti Lumionics Inc. | Method for patterning a coating on a surface and device including a patterned coating |
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| CN116583131A (zh) | 2017-04-26 | 2023-08-11 | Oti照明公司 | 用于图案化表面上覆层的方法和包括图案化覆层的装置 |
| JP7264488B2 (ja) | 2017-05-17 | 2023-04-25 | オーティーアイ ルミオニクス インコーポレーテッド | パターン化コーティングにわたって伝導性コーティングを選択的に堆積させるための方法および伝導性コーティングを含むデバイス |
| CN110603326B (zh) * | 2017-09-26 | 2024-03-08 | 宁波尚宁生物技术有限公司 | 扩增靶核酸的方法 |
| US11751415B2 (en) | 2018-02-02 | 2023-09-05 | Oti Lumionics Inc. | Materials for forming a nucleation-inhibiting coating and devices incorporating same |
| EP3768832B1 (en) * | 2018-03-21 | 2023-11-29 | F. Hoffmann-La Roche AG | Dna polymerases for efficient and effective incorporation of methylated-dntps |
| JP7320851B2 (ja) | 2018-05-07 | 2023-08-04 | オーティーアイ ルミオニクス インコーポレーテッド | 補助電極を提供するための方法および補助電極を含むデバイス |
| US11268140B2 (en) * | 2018-06-12 | 2022-03-08 | Shaofeng Ding | Delayed pyrophosphorolysis activated polymerization |
| JP2022508040A (ja) | 2018-11-23 | 2022-01-19 | オーティーアイ ルミオニクス インコーポレーテッド | 光透過領域を含むオプトエレクトロニクスデバイス |
| JP7390739B2 (ja) | 2019-03-07 | 2023-12-04 | オーティーアイ ルミオニクス インコーポレーテッド | 核生成抑制コーティングを形成するための材料およびそれを組み込んだデバイス |
| KR102831863B1 (ko) | 2019-04-18 | 2025-07-10 | 오티아이 루미오닉스 인크. | 핵 생성 억제 코팅 형성용 물질 및 이를 포함하는 디바이스 |
| JP7576337B2 (ja) | 2019-05-08 | 2024-11-01 | オーティーアイ ルミオニクス インコーポレーテッド | 核生成抑制コーティングを形成するための材料およびそれを組み込んだデバイス |
| US11832473B2 (en) | 2019-06-26 | 2023-11-28 | Oti Lumionics Inc. | Optoelectronic device including light transmissive regions, with light diffraction characteristics |
| CN114097102B (zh) | 2019-06-26 | 2023-11-03 | Oti照明公司 | 包括具有光衍射特征的光透射区域的光电设备 |
| CN114342068A (zh) | 2019-08-09 | 2022-04-12 | Oti照明公司 | 包含辅助电极和分区的光电子装置 |
| KR20260036028A (ko) | 2019-12-24 | 2026-03-13 | 오티아이 루미오닉스 인크. | 캡핑 층을 포함하는 발광 장치 및 그 제조방법 |
| CN111108860B (zh) * | 2020-01-19 | 2024-05-14 | 黑龙江丰沃非凡农业科技发展有限公司 | 一种仿形播种机 |
| WO2022123431A1 (en) | 2020-12-07 | 2022-06-16 | Oti Lumionics Inc. | Patterning a conductive deposited layer using a nucleation inhibiting coating and an underlying metallic coating |
| GB202102170D0 (en) * | 2021-02-16 | 2021-03-31 | Biofidelity Ltd | Polynucleotide detection |
| CN117904281B (zh) * | 2024-01-29 | 2024-11-22 | 上海科亦生物科技有限公司 | 检测ugt1a1基因多态性的引物组及试剂盒与方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| CA2035010C (en) * | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6270974B1 (en) * | 1998-03-13 | 2001-08-07 | Promega Corporation | Exogenous nucleic acid detection |
| US6159693A (en) * | 1998-03-13 | 2000-12-12 | Promega Corporation | Nucleic acid detection |
| US6312902B1 (en) * | 1998-03-13 | 2001-11-06 | Promega Corporation | Nucleic acid detection |
| US6391551B1 (en) * | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
| US6270973B1 (en) * | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
| US6277578B1 (en) * | 1998-03-13 | 2001-08-21 | Promega Corporation | Deploymerization method for nucleic acid detection of an amplified nucleic acid target |
| US7090975B2 (en) | 1998-03-13 | 2006-08-15 | Promega Corporation | Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection |
| US6335162B1 (en) * | 1998-03-13 | 2002-01-01 | Promega Corporation | Nucleic acid detection |
| US6268146B1 (en) * | 1998-03-13 | 2001-07-31 | Promega Corporation | Analytical methods and materials for nucleic acid detection |
| US6235480B1 (en) * | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
| US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| US6509157B1 (en) * | 1999-11-05 | 2003-01-21 | Roche Molecular Systems, Inc | 3 blocked nucleic acid amplification primers |
| WO2001075139A1 (en) * | 2000-04-03 | 2001-10-11 | Biolink Partners, Inc. | Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization |
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| WO2001062975A3 (en) | 2002-08-29 |
| ATE441726T1 (de) | 2009-09-15 |
| EP1259646B1 (en) | 2009-09-02 |
| US7105298B2 (en) | 2006-09-12 |
| JP2003526344A (ja) | 2003-09-09 |
| US20030092051A1 (en) | 2003-05-15 |
| ES2330408T3 (es) | 2009-12-10 |
| WO2001062975A9 (en) | 2002-10-24 |
| DE60139762D1 (de) | 2009-10-15 |
| AU4162101A (en) | 2001-09-03 |
| CA2395391A1 (en) | 2001-08-30 |
| WO2001062975A2 (en) | 2001-08-30 |
| JP4989004B2 (ja) | 2012-08-01 |
| US6534269B2 (en) | 2003-03-18 |
| CA2395391C (en) | 2011-08-02 |
| EP1259646A2 (en) | 2002-11-27 |
| EP2112233A1 (en) | 2009-10-28 |
| US20020127554A1 (en) | 2002-09-12 |
| EP2112233B1 (en) | 2013-10-23 |
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