JP2002502615A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2002502615A5 JP2002502615A5 JP2000530635A JP2000530635A JP2002502615A5 JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5 JP 2000530635 A JP2000530635 A JP 2000530635A JP 2000530635 A JP2000530635 A JP 2000530635A JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5
- Authority
- JP
- Japan
- Prior art keywords
- probes
- different
- fluorescence
- allele
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 description 120
- 238000000034 method Methods 0.000 description 92
- 108700028369 Alleles Proteins 0.000 description 53
- 230000003321 amplification Effects 0.000 description 51
- 238000003199 nucleic acid amplification method Methods 0.000 description 51
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 49
- 239000002751 oligonucleotide probe Substances 0.000 description 49
- 108020004414 DNA Proteins 0.000 description 44
- 238000002844 melting Methods 0.000 description 40
- 230000008018 melting Effects 0.000 description 40
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 39
- 108020004707 nucleic acids Proteins 0.000 description 39
- 102000039446 nucleic acids Human genes 0.000 description 39
- 150000007523 nucleic acids Chemical class 0.000 description 39
- 238000002189 fluorescence spectrum Methods 0.000 description 27
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 20
- 239000011541 reaction mixture Substances 0.000 description 19
- 239000003795 chemical substances by application Substances 0.000 description 15
- 108091093088 Amplicon Proteins 0.000 description 14
- 108010010803 Gelatin Proteins 0.000 description 13
- 238000000137 annealing Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 229920000159 gelatin Polymers 0.000 description 13
- 239000008273 gelatin Substances 0.000 description 13
- 235000019322 gelatine Nutrition 0.000 description 13
- 235000011852 gelatine desserts Nutrition 0.000 description 13
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 13
- 229920001213 Polysorbate 20 Polymers 0.000 description 12
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 11
- 238000010791 quenching Methods 0.000 description 11
- 101710163270 Nuclease Proteins 0.000 description 10
- 108010037497 3'-nucleotidase Proteins 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 7
- 239000000975 dye Substances 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 7
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 4
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 101150037123 APOE gene Proteins 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 101100216294 Danio rerio apoeb gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1859598A | 1998-02-04 | 1998-02-04 | |
| US09/018,595 | 1998-02-04 | ||
| PCT/US1999/000499 WO1999040226A2 (en) | 1998-02-04 | 1999-01-08 | Determination of a genotype of an amplification product at multiple allelic sites |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002502615A JP2002502615A (ja) | 2002-01-29 |
| JP2002502615A5 true JP2002502615A5 (https=) | 2006-02-16 |
| JP4388694B2 JP4388694B2 (ja) | 2009-12-24 |
Family
ID=21788757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000530635A Expired - Lifetime JP4388694B2 (ja) | 1998-02-04 | 1999-01-08 | 複数のアレル部位における増幅産物のゲノム型決定 |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US6154707A (https=) |
| EP (1) | EP1053348B1 (https=) |
| JP (1) | JP4388694B2 (https=) |
| AT (1) | ATE469982T1 (https=) |
| AU (1) | AU758463B2 (https=) |
| CA (1) | CA2318880C (https=) |
| DE (1) | DE69942444D1 (https=) |
| WO (1) | WO1999040226A2 (https=) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4034351B2 (ja) | 1996-04-25 | 2008-01-16 | バイオアレイ ソリューションズ エルエルシー | 粒子近接表面の光制御した動電学的アッセンブリ |
| AU741141B2 (en) * | 1997-11-04 | 2001-11-22 | Roche Diagnostics Gmbh | Specific and sensitive method for detecting nucleic acids |
| AU758463B2 (en) | 1998-02-04 | 2003-03-20 | Applied Biosystems, Llc | Determination of a genotype of an amplification product at multiple allelic sites |
| WO2001031062A1 (en) * | 1999-10-22 | 2001-05-03 | The Public Health Research Institute Of The City Of New York, Inc. | Assays for short sequence variants |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6660845B1 (en) | 1999-11-23 | 2003-12-09 | Epoch Biosciences, Inc. | Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof |
| US20040081959A9 (en) | 1999-12-08 | 2004-04-29 | Epoch Biosciences, Inc. | Fluorescent quenching detection reagents and methods |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| JP2001299392A (ja) * | 2000-04-25 | 2001-10-30 | Otsuka Pharmaceut Co Ltd | Ｃａｔｃｈ２２症候群検出方法 |
| CA2409774A1 (en) * | 2000-05-23 | 2001-11-29 | Variagenics, Inc. | Methods for genetic analysis of dna to detect sequence variances |
| US7435541B2 (en) | 2000-05-23 | 2008-10-14 | Sequenom, Inc. | Restriction enzyme genotyping |
| US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| DE60117556T2 (de) | 2000-06-21 | 2006-11-02 | Bioarray Solutions Ltd. | Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays |
| GB0016516D0 (en) * | 2000-07-06 | 2000-08-23 | Owen Mumford Ltd | Improvements relating to needle protection devices |
| US7267945B2 (en) * | 2001-03-26 | 2007-09-11 | Applera Corporation | Methods of determining the presence of polynucleotides employing amplification |
| US20030143554A1 (en) * | 2001-03-31 | 2003-07-31 | Berres Mark E. | Method of genotyping by determination of allele copy number |
| US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
| KR100944674B1 (ko) * | 2001-06-28 | 2010-03-04 | 노바티스 백신즈 앤드 다이아그노스틱스 인코포레이티드 | 파르보바이러스 ｂ19의 진단 분석 |
| EP1463825B1 (en) | 2001-10-15 | 2017-12-06 | BioArray Solutions Ltd. | Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection |
| JPWO2003035864A1 (ja) * | 2001-10-26 | 2005-02-10 | 松下電器産業株式会社 | 標的核酸の検出方法及び核酸プローブ |
| US20110111389A1 (en) * | 2001-11-07 | 2011-05-12 | Diagcor Bioscience Incorporation Limited | Rapid genotyping analysis for human papillomavirus and the device thereof |
| US7732138B2 (en) * | 2001-11-07 | 2010-06-08 | Diagcor Bioscience Incorporation Limited | Rapid genotyping analysis and the device thereof |
| US20050079493A1 (en) * | 2002-11-09 | 2005-04-14 | Tam Joseph Wing On | DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device |
| DE60323214D1 (de) * | 2002-03-05 | 2008-10-09 | Physiogenix Inc | Verfahren und zusammensetzungen für die pharmakologische und toxikologische auswertung von testsubstanzen |
| US7176002B2 (en) * | 2002-05-16 | 2007-02-13 | Applera Corporation | Universal-tagged oligonucleotide primers and methods of use |
| EP1509624A2 (en) * | 2002-05-31 | 2005-03-02 | Secretary, Department Of Atomic Energy | Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands |
| CN105258988A (zh) | 2002-06-20 | 2016-01-20 | 徕卡病理系统墨尔本控股有限公司 | 带有排放机构的生物反应装置 |
| AU2003901871A0 (en) * | 2003-03-31 | 2003-05-08 | Vision Biosystems Limited | A method and apparatus for fluid dispensation, preparation and dilation |
| WO2004018626A2 (en) * | 2002-08-21 | 2004-03-04 | Epoch Biosciences, Inc. | Abasic site endonuclease assay |
| US7526114B2 (en) | 2002-11-15 | 2009-04-28 | Bioarray Solutions Ltd. | Analysis, secure access to, and transmission of array images |
| WO2004055709A2 (en) * | 2002-12-13 | 2004-07-01 | Applera Corporation | Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species |
| US7851150B2 (en) * | 2002-12-18 | 2010-12-14 | Third Wave Technologies, Inc. | Detection of small nucleic acids |
| WO2004061124A2 (en) | 2002-12-31 | 2004-07-22 | Mmi Genomics, Inc. | Compositions, methods, and systems for inferring bovine breed |
| US7858301B2 (en) * | 2003-05-07 | 2010-12-28 | Bioarray Solutions, Ltd. | Method of probe design for nucleic acid analysis by multiplexed hybridization |
| US20050089889A1 (en) * | 2003-06-20 | 2005-04-28 | Ramsing Niels B. | Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same |
| US20050038776A1 (en) * | 2003-08-15 | 2005-02-17 | Ramin Cyrus | Information system for biological and life sciences research |
| WO2005029705A2 (en) | 2003-09-18 | 2005-03-31 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
| US20050221357A1 (en) * | 2003-09-19 | 2005-10-06 | Mark Shannon | Normalization of gene expression data |
| US7417726B2 (en) * | 2003-09-19 | 2008-08-26 | Applied Biosystems Inc. | Normalization of data using controls |
| CA2539824C (en) | 2003-09-22 | 2015-02-03 | Xinwen Wang | Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules |
| US7332280B2 (en) * | 2003-10-14 | 2008-02-19 | Ronald Levy | Classification of patients having diffuse large B-cell lymphoma based upon gene expression |
| CA2899287A1 (en) | 2003-10-28 | 2005-05-12 | Bioarray Solutions Ltd. | Optimization of gene expression analysis using immobilized capture probes |
| WO2005045060A2 (en) | 2003-10-29 | 2005-05-19 | Bioarray Solutions, Ltd. | Multiplexed nucleic acid analysis by fragmentation of double-stranded dna |
| US7439341B2 (en) | 2003-11-14 | 2008-10-21 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| EP1561823A1 (de) * | 2004-02-04 | 2005-08-10 | Biotez Berlin-Buch GmbH | Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens |
| US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
| WO2006034387A1 (en) * | 2004-09-21 | 2006-03-30 | Applera Corporation | TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs) |
| US20060111915A1 (en) * | 2004-11-23 | 2006-05-25 | Applera Corporation | Hypothesis generation |
| US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
| WO2006061994A1 (ja) * | 2004-12-08 | 2006-06-15 | Takeshi Yamamoto | 遺伝子配列検査法 |
| US20060166238A1 (en) * | 2004-12-22 | 2006-07-27 | Ramsing Niels B | Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same |
| JP2008545659A (ja) | 2005-05-20 | 2008-12-18 | インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド | オリゴヌクレオチドを標識する化合物及び方法 |
| US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
| EP2477029A1 (en) | 2005-06-02 | 2012-07-18 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| WO2007024800A2 (en) * | 2005-08-22 | 2007-03-01 | Applera Corporation | Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other |
| WO2007147079A2 (en) * | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Rare cell analysis using sample splitting and dna tags |
| US20080090239A1 (en) * | 2006-06-14 | 2008-04-17 | Daniel Shoemaker | Rare cell analysis using sample splitting and dna tags |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| WO2007147074A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Use of highly parallel snp genotyping for fetal diagnosis |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| EP2589668A1 (en) * | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| DE602006008150D1 (de) | 2006-10-12 | 2009-09-10 | Bio Rad Pasteur | Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren |
| DE102007013099A1 (de) | 2007-03-14 | 2008-09-18 | Aj Innuscreen Gmbh | Verfahren und Testkit zum schnellen Nachweis spezifischer Nukleinsäuresequenzen, insbesondere zum Nachweis von Mutationen oder SNP's |
| EP2116614A1 (en) * | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8383346B2 (en) * | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| EP3023494B1 (en) * | 2008-06-13 | 2018-11-28 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| HUE031848T2 (en) | 2008-09-20 | 2017-08-28 | Univ Leland Stanford Junior | Non-invasive diagnosis of fetal aneuploidy by sequencing |
| JP5709840B2 (ja) * | 2009-04-13 | 2015-04-30 | キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． | 動的シグナルの相関分析による、パターン認識、機械学習、および自動遺伝子型分類の迅速な方法 |
| US20110312503A1 (en) | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
| AU2011230496B2 (en) | 2010-03-26 | 2015-09-17 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
| US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| AU2011299233B2 (en) | 2010-09-07 | 2016-09-15 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| US20120083035A1 (en) | 2010-09-30 | 2012-04-05 | Dharmacon, Inc. | Modified Cell Lines for Increasing Lentiviral Titers |
| US9617588B2 (en) | 2010-10-05 | 2017-04-11 | Thermo Fisher Scientific Baltics Uab | Enzyme mixture |
| EP2852682B1 (en) | 2012-05-21 | 2017-10-04 | Fluidigm Corporation | Single-particle analysis of particle populations |
| WO2017177025A1 (en) * | 2016-04-06 | 2017-10-12 | Life Technologies Corporation | Compositions, methods, and kits for synthesis and detection of nucleic acids |
| JP6846636B2 (ja) * | 2017-02-28 | 2021-03-24 | パナソニックＩｐマネジメント株式会社 | 核酸検出用オリゴヌクレオチド |
| WO2022155397A1 (en) * | 2021-01-15 | 2022-07-21 | Nuprobe Usa, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5075216A (en) | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
| WO1992001814A2 (en) | 1990-07-24 | 1992-02-06 | F. Hoffmann-La-Roche Ag | The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5403707A (en) * | 1993-05-14 | 1995-04-04 | Eastman Kodak Company | Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5834181A (en) | 1994-07-28 | 1998-11-10 | Genzyme Corporation | High throughput screening method for sequences or genetic alterations in nucleic acids |
| EP0803058B1 (en) * | 1994-12-30 | 2008-11-05 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| DE19524579C2 (de) * | 1995-07-06 | 1997-12-18 | Daimler Benz Ag | Transformator als Strombegrenzer |
| US5759781A (en) * | 1995-12-22 | 1998-06-02 | Yale University | Multiparametric fluorescence in situ hybridization |
| US5792610A (en) * | 1996-05-01 | 1998-08-11 | Biorad Laboratories, Inc. | Method for conducting multiparametric fluorescence in situ hybridization |
| EP0892808B1 (en) * | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
| US6015667A (en) * | 1996-06-03 | 2000-01-18 | The Perkin-Emer Corporation | Multicomponent analysis method including the determination of a statistical confidence interval |
| US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| AU758463B2 (en) * | 1998-02-04 | 2003-03-20 | Applied Biosystems, Llc | Determination of a genotype of an amplification product at multiple allelic sites |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
-
1999
- 1999-01-08 AU AU23144/99A patent/AU758463B2/en not_active Ceased
- 1999-01-08 DE DE69942444T patent/DE69942444D1/de not_active Expired - Lifetime
- 1999-01-08 WO PCT/US1999/000499 patent/WO1999040226A2/en not_active Ceased
- 1999-01-08 AT AT99903026T patent/ATE469982T1/de not_active IP Right Cessation
- 1999-01-08 CA CA2318880A patent/CA2318880C/en not_active Expired - Fee Related
- 1999-01-08 JP JP2000530635A patent/JP4388694B2/ja not_active Expired - Lifetime
- 1999-01-08 EP EP99903026A patent/EP1053348B1/en not_active Expired - Lifetime
- 1999-06-03 US US09/324,709 patent/US6154707A/en not_active Expired - Lifetime
-
2002
- 2002-03-21 US US10/104,774 patent/US6884583B2/en not_active Expired - Lifetime
-
2003
- 2003-06-04 US US10/455,150 patent/US7132239B2/en not_active Expired - Fee Related
-
2006
- 2006-11-03 US US11/592,716 patent/US20080187913A1/en not_active Abandoned
-
2009
- 2009-11-13 US US12/618,686 patent/US20120053069A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002502615A5 (https=) | ||
| CA2318880A1 (en) | Determination of a genotype of an amplification product at multiple allelic sites | |
| US6270967B1 (en) | Method for detecting a nucleic acid base sequence | |
| US7803543B2 (en) | Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe | |
| US8323929B2 (en) | Methods for detecting nucleic acid sequence variations | |
| JP5637850B2 (ja) | 標的核酸配列の増幅方法、それを用いた変異の検出方法、および、それに用いる試薬 | |
| JP2014501533A (ja) | 突然変異解析 | |
| GB2252407A (en) | Detection of variant nucleotides in a target sequence | |
| JP2001517936A (ja) | 選択的連結及び増幅方法 | |
| KR102265417B1 (ko) | 단일염기다형성 다중 분석용 프라이머 | |
| JP2007530026A (ja) | 核酸配列決定 | |
| US20030165913A1 (en) | Methods for detecting nucleic acid sequence variations | |
| JP7391321B2 (ja) | アクネ菌のｄｎａタイプの判別用オリゴヌクレオチドセット、キット及びアクネ菌のｄｎａタイプの判別方法 | |
| JP5831093B2 (ja) | Ｃ型慢性肝炎に対する治療効果を予測するためのプローブ | |
| US20030104421A1 (en) | Methods and compositions for nucleic acid amplification | |
| JP2023036344A (ja) | ＳＡＲＳ－ＣｏＶ－２の型を判定する方法、前記方法に用いるプローブセット及び前記方法に用いるプライマープローブセット | |
| EP4172361B1 (en) | Detection of methylation status | |
| EP4613876A1 (en) | Nucleic acid quantification using universal detection probes | |
| JPWO2010113452A1 (ja) | 遺伝子型の識別方法 | |
| JPWO2020229510A5 (https=) | ||
| US20050260607A1 (en) | Single nucleotide polymorphism genotyping | |
| EP4155418A1 (en) | Single nucleic acid for real-time detection for snp analysis of apoe gene and detection method using the same | |
| US20230098408A1 (en) | Single nucleic acid for real-time detection for snp analysis of apoe gene and detection method using the same | |
| JP4706223B2 (ja) | 塩基多型の同定方法 | |
| JP5504676B2 (ja) | 遺伝子型の識別方法 |