CA2318880C - Determination of a genotype of an amplification product at multiple allelic sites - Google Patents

Determination of a genotype of an amplification product at multiple allelic sites Download PDF

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Publication number
CA2318880C
CA2318880C CA2318880A CA2318880A CA2318880C CA 2318880 C CA2318880 C CA 2318880C CA 2318880 A CA2318880 A CA 2318880A CA 2318880 A CA2318880 A CA 2318880A CA 2318880 C CA2318880 C CA 2318880C
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Canada
Prior art keywords
allelic
probes
fluorescence
different
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2318880A
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English (en)
French (fr)
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CA2318880A1 (en
Inventor
Kenneth J. Livak
Federico Goodsaid
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Applied Biosystems LLC
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Publication of CA2318880C publication Critical patent/CA2318880C/en
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Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Amplifiers (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
CA2318880A 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites Expired - Fee Related CA2318880C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1859598A 1998-02-04 1998-02-04
US09/018,595 1998-02-04
PCT/US1999/000499 WO1999040226A2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Publications (2)

Publication Number Publication Date
CA2318880A1 CA2318880A1 (en) 1999-08-12
CA2318880C true CA2318880C (en) 2010-07-27

Family

ID=21788757

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2318880A Expired - Fee Related CA2318880C (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Country Status (8)

Country Link
US (5) US6154707A (https=)
EP (1) EP1053348B1 (https=)
JP (1) JP4388694B2 (https=)
AT (1) ATE469982T1 (https=)
AU (1) AU758463B2 (https=)
CA (1) CA2318880C (https=)
DE (1) DE69942444D1 (https=)
WO (1) WO1999040226A2 (https=)

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Also Published As

Publication number Publication date
WO1999040226A2 (en) 1999-08-12
US6884583B2 (en) 2005-04-26
AU758463B2 (en) 2003-03-20
US20020164630A1 (en) 2002-11-07
JP2002502615A (ja) 2002-01-29
US20040053302A1 (en) 2004-03-18
AU2314499A (en) 1999-08-23
US20120053069A1 (en) 2012-03-01
ATE469982T1 (de) 2010-06-15
EP1053348A2 (en) 2000-11-22
EP1053348B1 (en) 2010-06-02
US6154707A (en) 2000-11-28
DE69942444D1 (de) 2010-07-15
US7132239B2 (en) 2006-11-07
US20080187913A1 (en) 2008-08-07
CA2318880A1 (en) 1999-08-12
WO1999040226A3 (en) 2000-08-17
JP4388694B2 (ja) 2009-12-24

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Effective date: 20180108