ATE469982T1 - Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen - Google Patents

Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Info

Publication number
ATE469982T1
ATE469982T1 AT99903026T AT99903026T ATE469982T1 AT E469982 T1 ATE469982 T1 AT E469982T1 AT 99903026 T AT99903026 T AT 99903026T AT 99903026 T AT99903026 T AT 99903026T AT E469982 T1 ATE469982 T1 AT E469982T1
Authority
AT
Austria
Prior art keywords
fluorescer
fluorescence
allelic
target sequence
sites
Prior art date
Application number
AT99903026T
Other languages
German (de)
English (en)
Inventor
Kenneth Livak
Federico Goodsaid
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE469982T1 publication Critical patent/ATE469982T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Amplifiers (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT99903026T 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen ATE469982T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1859598A 1998-02-04 1998-02-04
PCT/US1999/000499 WO1999040226A2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Publications (1)

Publication Number Publication Date
ATE469982T1 true ATE469982T1 (de) 2010-06-15

Family

ID=21788757

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99903026T ATE469982T1 (de) 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Country Status (8)

Country Link
US (5) US6154707A (https=)
EP (1) EP1053348B1 (https=)
JP (1) JP4388694B2 (https=)
AT (1) ATE469982T1 (https=)
AU (1) AU758463B2 (https=)
CA (1) CA2318880C (https=)
DE (1) DE69942444D1 (https=)
WO (1) WO1999040226A2 (https=)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4034351B2 (ja) 1996-04-25 2008-01-16 バイオアレイ ソリューションズ エルエルシー 粒子近接表面の光制御した動電学的アッセンブリ
AU741141B2 (en) * 1997-11-04 2001-11-22 Roche Diagnostics Gmbh Specific and sensitive method for detecting nucleic acids
AU758463B2 (en) 1998-02-04 2003-03-20 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
WO2001031062A1 (en) * 1999-10-22 2001-05-03 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US20040081959A9 (en) 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
JP2001299392A (ja) * 2000-04-25 2001-10-30 Otsuka Pharmaceut Co Ltd Ｃａｔｃｈ２２症候群検出方法
CA2409774A1 (en) * 2000-05-23 2001-11-29 Variagenics, Inc. Methods for genetic analysis of dna to detect sequence variances
US7435541B2 (en) 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
DE60117556T2 (de) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
KR100944674B1 (ko) * 2001-06-28 2010-03-04 노바티스 백신즈 앤드 다이아그노스틱스 인코포레이티드 파르보바이러스 ｂ19의 진단 분석
EP1463825B1 (en) 2001-10-15 2017-12-06 BioArray Solutions Ltd. Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
JPWO2003035864A1 (ja) * 2001-10-26 2005-02-10 松下電器産業株式会社 標的核酸の検出方法及び核酸プローブ
US20110111389A1 (en) * 2001-11-07 2011-05-12 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis for human papillomavirus and the device thereof
US7732138B2 (en) * 2001-11-07 2010-06-08 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis and the device thereof
US20050079493A1 (en) * 2002-11-09 2005-04-14 Tam Joseph Wing On DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device
DE60323214D1 (de) * 2002-03-05 2008-10-09 Physiogenix Inc Verfahren und zusammensetzungen für die pharmakologische und toxikologische auswertung von testsubstanzen
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1509624A2 (en) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
CN105258988A (zh) 2002-06-20 2016-01-20 徕卡病理系统墨尔本控股有限公司 带有排放机构的生物反应装置
AU2003901871A0 (en) * 2003-03-31 2003-05-08 Vision Biosystems Limited A method and apparatus for fluid dispensation, preparation and dilation
WO2004018626A2 (en) * 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
WO2004055709A2 (en) * 2002-12-13 2004-07-01 Applera Corporation Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species
US7851150B2 (en) * 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
WO2004061124A2 (en) 2002-12-31 2004-07-22 Mmi Genomics, Inc. Compositions, methods, and systems for inferring bovine breed
US7858301B2 (en) * 2003-05-07 2010-12-28 Bioarray Solutions, Ltd. Method of probe design for nucleic acid analysis by multiplexed hybridization
US20050089889A1 (en) * 2003-06-20 2005-04-28 Ramsing Niels B. Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
US20050038776A1 (en) * 2003-08-15 2005-02-17 Ramin Cyrus Information system for biological and life sciences research
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
US20050221357A1 (en) * 2003-09-19 2005-10-06 Mark Shannon Normalization of gene expression data
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
CA2539824C (en) 2003-09-22 2015-02-03 Xinwen Wang Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US7332280B2 (en) * 2003-10-14 2008-02-19 Ronald Levy Classification of patients having diffuse large B-cell lymphoma based upon gene expression
CA2899287A1 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
WO2005045060A2 (en) 2003-10-29 2005-05-19 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
EP1561823A1 (de) * 2004-02-04 2005-08-10 Biotez Berlin-Buch GmbH Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
WO2006034387A1 (en) * 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
US20060111915A1 (en) * 2004-11-23 2006-05-25 Applera Corporation Hypothesis generation
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
WO2006061994A1 (ja) * 2004-12-08 2006-06-15 Takeshi Yamamoto 遺伝子配列検査法
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
JP2008545659A (ja) 2005-05-20 2008-12-18 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド オリゴヌクレオチドを標識する化合物及び方法
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
WO2007024800A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
WO2007147079A2 (en) * 2006-06-14 2007-12-21 Living Microsystems, Inc. Rare cell analysis using sample splitting and dna tags
US20080090239A1 (en) * 2006-06-14 2008-04-17 Daniel Shoemaker Rare cell analysis using sample splitting and dna tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
WO2007147074A2 (en) 2006-06-14 2007-12-21 Living Microsystems, Inc. Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (en) * 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
DE602006008150D1 (de) 2006-10-12 2009-09-10 Bio Rad Pasteur Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren
DE102007013099A1 (de) 2007-03-14 2008-09-18 Aj Innuscreen Gmbh Verfahren und Testkit zum schnellen Nachweis spezifischer Nukleinsäuresequenzen, insbesondere zum Nachweis von Mutationen oder SNP's
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
EP3023494B1 (en) * 2008-06-13 2018-11-28 Codexis, Inc. Method of synthesizing polynucleotide variants
HUE031848T2 (en) 2008-09-20 2017-08-28 Univ Leland Stanford Junior Non-invasive diagnosis of fetal aneuploidy by sequencing
JP5709840B2 (ja) * 2009-04-13 2015-04-30 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． 動的シグナルの相関分析による、パターン認識、機械学習、および自動遺伝子型分類の迅速な方法
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011299233B2 (en) 2010-09-07 2016-09-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US20120083035A1 (en) 2010-09-30 2012-04-05 Dharmacon, Inc. Modified Cell Lines for Increasing Lentiviral Titers
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations
WO2017177025A1 (en) * 2016-04-06 2017-10-12 Life Technologies Corporation Compositions, methods, and kits for synthesis and detection of nucleic acids
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックＩｐマネジメント株式会社 核酸検出用オリゴヌクレオチド
WO2022155397A1 (en) * 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
WO1992001814A2 (en) 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5834181A (en) 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
EP0803058B1 (en) * 1994-12-30 2008-11-05 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
DE19524579C2 (de) * 1995-07-06 1997-12-18 Daimler Benz Ag Transformator als Strombegrenzer
US5759781A (en) * 1995-12-22 1998-06-02 Yale University Multiparametric fluorescence in situ hybridization
US5792610A (en) * 1996-05-01 1998-08-11 Biorad Laboratories, Inc. Method for conducting multiparametric fluorescence in situ hybridization
EP0892808B1 (en) * 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US6015667A (en) * 1996-06-03 2000-01-18 The Perkin-Emer Corporation Multicomponent analysis method including the determination of a statistical confidence interval
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
AU758463B2 (en) * 1998-02-04 2003-03-20 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
WO1999040226A2 (en) 1999-08-12
CA2318880C (en) 2010-07-27
US6884583B2 (en) 2005-04-26
AU758463B2 (en) 2003-03-20
US20020164630A1 (en) 2002-11-07
JP2002502615A (ja) 2002-01-29
US20040053302A1 (en) 2004-03-18
AU2314499A (en) 1999-08-23
US20120053069A1 (en) 2012-03-01
EP1053348A2 (en) 2000-11-22
EP1053348B1 (en) 2010-06-02
US6154707A (en) 2000-11-28
DE69942444D1 (de) 2010-07-15
US7132239B2 (en) 2006-11-07
US20080187913A1 (en) 2008-08-07
CA2318880A1 (en) 1999-08-12
WO1999040226A3 (en) 2000-08-17
JP4388694B2 (ja) 2009-12-24

Similar Documents

Publication Publication Date Title
ATE469982T1 (de) Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
US7078168B2 (en) Method for determining allele frequencies
US7223536B2 (en) Methods for detecting single nucleotide polymorphisms
Germer et al. High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR
US7879543B2 (en) Method of detecting mutations associated with thrombosis
DE602005027067D1 (de) Nachweis chromosomaler störungen
US10590468B2 (en) Method for methylation analysis
EP1241266A4 (en) METHOD FOR DETECTING NUCLEIC ACID POLYMORPHISMS
Ørum PCR clamping
Petersen et al. Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms
ES2190925T3 (es) Gen del cancer de prostata.
RU2012129362A (ru) Обнаружение мишени tsg праймером
JP2002502615A5 (https=)
Pettersson et al. Molecular haplotype determination using allele-specific PCR and pyrosequencing technology
DE60332775D1 (de) Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen
Ortiz et al. Generic platform for the multiplexed targeted electrochemical detection of osteoporosis-associated single nucleotide polymorphisms using recombinase polymerase solid-phase primer elongation and ferrocene-modified nucleoside triphosphates
Wang et al. DNAzyme‐Based Probes for Telomerase Detection in Early‐Stage Cancer Diagnosis
Nakamura et al. Simple and accurate determination of CYP2D6 gene copy number by a loop-mediated isothermal amplification method and an electrochemical DNA chip
Mushailov et al. Assay development and validation of an 8‐SNP multiplex test to predict eye and skin coloration
WO1999005313A3 (en) Method for typing of minor histocompatibility antigen ha-1
young Mun et al. SNPs detection by a single-strand specific nuclease on a PNA zip-code microarray
CA2451394A1 (en) Il-4 receptor sequence variation associated with type 1 diabetes
Storts Alternative probe-based detection systems in quantitative PCR
Masaki et al. A kinetically controlled, isothermal method for the detection of single nucleotide mismatches
Álvarez-Iglesias et al. Genotyping coding region mtDNA SNPs for Asian and Native American haplogroup assignation

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties