JP2003073186A - Natural amino acid fertilizer derived from silk protein and its production method - Google Patents
Natural amino acid fertilizer derived from silk protein and its production methodInfo
- Publication number
- JP2003073186A JP2003073186A JP2001310730A JP2001310730A JP2003073186A JP 2003073186 A JP2003073186 A JP 2003073186A JP 2001310730 A JP2001310730 A JP 2001310730A JP 2001310730 A JP2001310730 A JP 2001310730A JP 2003073186 A JP2003073186 A JP 2003073186A
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- natural amino
- silk
- solution
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Fertilizers (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、繭を所定の方法で
処理して得られる、絹タンパク質由来の天然アミノ酸肥
料及びその製造方法に関するものである。TECHNICAL FIELD The present invention relates to a natural amino acid fertilizer derived from silk protein, which is obtained by treating cocoons by a predetermined method, and a method for producing the same.
【０００２】[0002]
【従来の技術】従来、天然アミノ酸肥料としては、魚
粕、植物性加工食品の粕類、鶏糞や家畜等の排泄物等に
よる天然アミノ酸源肥料が知られている。また、絹は天
然タンパク質であるが、そのままの状態（繭、さなぎ、
絹フィブロイン、絹糸等）では土壌にいれても生分解さ
れにくい。このため、窒素源及び／またはアミノ酸源肥
料として絹を施肥する習慣はほとんどない。近年、絹の
微粉砕物または加水分解物を化粧料、食料等に利用する
ことは提案されているが、農業用途においては天然アミ
ノ酸源肥料としての利用は知られていない。2. Description of the Related Art Conventionally, as a natural amino acid fertilizer, a natural amino acid source fertilizer such as fish meal, plant processed food meal, chicken manure, excrements of livestock and the like has been known. Also, silk is a natural protein, but as it is (cocoon, pupa,
Silk fibroin, silk thread, etc.) are not easily biodegraded even when placed in soil. For this reason, there is little practice in applying silk as a nitrogen source and / or amino acid source fertilizer. In recent years, it has been proposed to use a finely pulverized product or a hydrolyzed product of silk for cosmetics, foods, etc., but its use as a natural amino acid source fertilizer is not known for agricultural use.
【０００３】[0003]
【発明が解決しようとする課題】本発明は、本来多種の
アミノ酸構成単位を含むにもかかわらず、天然アミノ酸
源肥料としては検討されなかった絹タンパク質のアミノ
酸成分を肥料として有効利用することを提案するもので
あり、絹産業の副産物として多量に排出されるクズ繭、
紡糸、製織工程等で生じる糸クズ等を有効利用すると共
に、繭の中にあるさなぎも有効利用することによって、
大巾なコスト低減を実現した、絹タンパク質由来の天然
アミノ酸肥料を提供するものである。DISCLOSURE OF THE INVENTION The present invention proposes to effectively utilize the amino acid component of silk protein as a fertilizer, which originally has not been examined as a natural amino acid source fertilizer, although it originally contains various amino acid constituent units. The waste cocoons, which are produced in large quantities as by-products of the silk industry,
By effectively using the thread scraps generated in the spinning, weaving process, etc., and also effectively using the pupa in the cocoon,
The present invention provides a natural amino acid fertilizer derived from silk protein, which realizes a significant cost reduction.
【０００４】[0004]
【課題を解決するための手段】繭を等量体積のお湯で煮
た後、規定量の塩酸を加えて加水分解し、これから得ら
れたアミノ酸溶液を水酸化ナトリウムで中和、濃縮す
る。この濃縮液を透析してアミノ酸溶液を分離、抽出
し、これを凍結乾燥して粉末のシルクパウダーを作製す
る。他方、上記工程で分解されなかったさなぎを取り出
してすり潰し、再度上記工程により酸加水分解し、所定
の方法で脱脂、粉末化した後、上記シルクパウダーとさ
なぎ粉末を混合して天然アミノ酸肥料を得る。尚、シル
クパウダーの平均分子量は３００以下とする。Means for Solving the Problems After boiling cocoons in an equal volume of hot water, a prescribed amount of hydrochloric acid is added for hydrolysis, and the resulting amino acid solution is neutralized and concentrated with sodium hydroxide. This concentrated solution is dialyzed to separate and extract an amino acid solution, which is freeze-dried to produce a powder silk powder. On the other hand, the pupae that have not been decomposed in the above step are taken out and ground, and again subjected to acid hydrolysis in the above step, degreased and powdered by a predetermined method, and then the silk powder and pupa powder are mixed to obtain a natural amino acid fertilizer. . The average molecular weight of the silk powder is 300 or less.
【０００５】[0005]
【発明の実施の形態】以下、本発明をより具体的に説明
する。
（イ）繭と等量体積（外観体積）のお湯で繭を煮る。こ
れにより繭の中の空気の一部とお湯が入れ替わり、繭が
水中に沈む。
（ロ）上記お湯の中に規定量の塩酸を加え、１０５℃で
３０時間位放置する。
（ハ）繭の糸の部分は加水分解され、アミノ酸溶液とな
り、さなぎは一部分解されるが、下に残る状態となるの
で加水分解されなかったさなぎを取り出す。
（ニ）アミノ酸溶液を水酸化ナトリウムで中和し濃縮す
ると、食塩とアミノ酸溶液の混合物となる。
（ホ）上記濃縮液を透析し、食塩とアミノ酸溶液を分離
し、アミノ酸溶液を抽出する。
（ヘ）アミノ酸溶液を凍結乾燥し、粉末のシルクパウダ
ーを作製する。
（ト）上記加水分解によって分解されずに残ったさなぎ
をすり潰し、再度お湯で加熱し、規定量の塩酸を加えて
酸加水分解する。
（チ）加水分解されたさなぎには脂肪がかなり含まれて
いるので、脱脂を行う。脱脂の方法は、加水分解したも
のを２日間位放置しておくと、脂肪が分離するので分離
した脂肪を取り除く。脱脂の方法は上記の他に、ろ過
法、活性炭で除去する方法、活性酸素による脱脂法等い
ろいろの方法がある。脱脂の目的は、さなぎ特有の強い
臭いを低減するためであり、肥料として必ずしも不適当
あるいは不必要というものではないので、必ず除去しな
けれはならないというものではない。
（リ）さなぎを凍結乾燥し、さなぎ粉末とする。さなぎ
粉末はタンパク質であり、土壌中でアミノ酸に分解され
る。
（ヌ）粉末のシルクパウダーとさなぎ粉末を混合し、絹
タンパク質由来の天然アミノ酸肥料が完成する。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail below. (B) Boil the cocoon with hot water of the same volume (appearance volume) as the cocoon. This replaces part of the air in the cocoon with the hot water, causing the cocoon to sink into the water. (B) A prescribed amount of hydrochloric acid is added to the hot water and left at 105 ° C. for about 30 hours. (C) The cocoon thread part is hydrolyzed into an amino acid solution, and the pupa is partly decomposed, but remains in the bottom, so the pupa not hydrolyzed is taken out. (D) When the amino acid solution is neutralized with sodium hydroxide and concentrated, it becomes a mixture of salt and amino acid solution. (E) The above concentrated solution is dialyzed, the salt and the amino acid solution are separated, and the amino acid solution is extracted. (F) Lyophilize the amino acid solution to prepare powdered silk powder. (G) The pupae that have not been decomposed by the above-mentioned hydrolysis are ground and then heated again with hot water, and a prescribed amount of hydrochloric acid is added to carry out acid hydrolysis. (H) Since the hydrolyzed pupa contains a large amount of fat, it should be defatted. As for the degreasing method, if the hydrolyzed product is left to stand for about 2 days, the fat will separate, so the separated fat is removed. In addition to the above, there are various degreasing methods such as a filtration method, a method of removing with activated carbon, and a degreasing method with active oxygen. The purpose of degreasing is to reduce the strong odor peculiar to pupa and is not necessarily unsuitable or unnecessary as a fertilizer, so it does not mean that it must be removed. (I) Lyophilize the pupa to give pupa powder. Peanut powder is a protein that is broken down into amino acids in soil. (Nu) Powder silk powder and pupa powder are mixed to complete a natural amino acid fertilizer derived from silk protein.
【０００６】粉末シルクパウダーの平均分子量を３００
以下とするには、濃縮液を透析分離したアミノ酸溶液を
凍結乾燥すると分子量の小さいシルクパウダーが得られ
る。平均分子量を３００以下とする理由は、この天然ア
ミノ酸肥料を水溶性肥料として用いる場合、分子量が大
きいとゲル化しやすくなり、液肥として使用しにくくな
るからである。The average molecular weight of powdered silk powder is 300
For the following, a silk powder having a small molecular weight can be obtained by freeze-drying the amino acid solution obtained by dialysis of the concentrated solution. The reason for setting the average molecular weight to 300 or less is that when this natural amino acid fertilizer is used as a water-soluble fertilizer, if the molecular weight is large, gelation tends to occur and it becomes difficult to use it as liquid fertilizer.
【０００７】本発明に供される絹タンパク質原料として
は、さなぎ入りの繭だけではなく、紡糸あるいは製織工
程等で生じる糸クズ等も利用できる。これら産業廃棄物
となる繭クズ、糸クズ等を利用すれば、廃棄物の減量に
なり環境にも好ましいと同時にコスト低減にもなって経
済的である。As the silk protein raw material to be used in the present invention, not only cocoons containing pupae but also thread scraps generated in the spinning or weaving process can be used. By using cocoon scraps, lint scraps, and the like that are industrial wastes, the amount of wastes is reduced, which is environmentally friendly and economical, as well as costly.
【０００８】酸加水分解は、塩酸を用いる方法の他に、
硫酸、リン酸等他の酸によって行うこともできるし、ア
ルカリを用いて行うこともできる。いずれによる場合も
中和、脱塩の処理が必要である。Acid hydrolysis is not limited to the method using hydrochloric acid,
It can be carried out using another acid such as sulfuric acid or phosphoric acid, or can be carried out using an alkali. In either case, neutralization and desalting treatments are required.
【０００９】繭は絹糸がセリシンで固められたものであ
り、これを加水分解すると、フィブロインとセリシンに
分離される。本発明においては、この両方共肥料として
利用するものであるが、参考として下表１にフィブロイ
ン及びセリシンのアミノ酸残基及び窒素量組成を示す。
A cocoon is a silk thread hardened with sericin, and when hydrolyzed, it is separated into fibroin and sericin. In the present invention, both of these are used as fertilizers, and the amino acid residues and nitrogen content compositions of fibroin and sericin are shown in Table 1 below for reference.
【００１０】本発明の天然アミノ酸肥料は粉末として施
肥してもよいし、また水溶性なので葉面等に液肥として
散布してもよい。液肥は、施肥する植物によっても異な
るが、通常水１リットルに対し１〜５０ｇの１０００倍
〜２０倍希釈即ち０．１％〜５％水溶液に調整するのが
よい。施肥方法は、植物の種類によっても異なり、また
施肥回数、施肥時期等も特に限定されない。施肥対象植
物も特に限定されず、数例を挙げれば、小松菜、レタ
ス、春菊、ホウレン草、白菜、キャベツ、チンゲンサ
イ、ネギ、大根、人参、ナス、トマト、キュウリ、メロ
ン等の種々の野菜類、稲等の穀物、リンゴ、梨等の果樹
類、さらには鑑賞用花類等の肥料として使用することが
できる。The natural amino acid fertilizer of the present invention may be applied as a powder, or may be sprayed as a liquid fertilizer on the leaves because it is water-soluble. Although the liquid fertilizer varies depending on the plant to be fertilized, it is usually preferable to adjust it to 1 to 50 g of 1000 times to 20 times dilution, that is, 0.1% to 5% aqueous solution per 1 liter of water. The fertilization method differs depending on the type of plant, and the number of fertilizations, the timing of fertilization, etc. are not particularly limited. The plants to be fertilized are not particularly limited, and, to name a few examples, various vegetables such as komatsuna, lettuce, garland chrysanthemum, spinach, Chinese cabbage, cabbage, bok choy, green onion, radish, carrot, eggplant, tomato, cucumber, melon, rice, etc. It can be used as a fertilizer such as grains such as, fruit trees such as apples and pears, and ornamental flowers.
【００１１】野菜類の場合には、施肥１回につき、本発
明のアミノ酸肥料を、３．３ｍ２当り粉末量換算で通常
１ｇ〜５ｇ施肥すれば、栄養のある野菜が育つと共に、
葉色が濃く、野菜の色が鮮やかになり、光合成促進効果
があり、また収穫後の日持ちも長くなるという効果があ
る。例えば、野菜類については、発芽時、苗の植付け
時、植え替え時等では０．２％程度の液肥を用いること
ができ、葉面散布では葉の表面が濡れる程度、根元散布
では株元がしっとり濡れる程度に散布する。また、移植
後の葉面散布、根元散布では、０．１％程度の液肥を用
いることができ、一週間に一回程度の割合で、葉の表面
が濡れる程度、あるいは株元がしっとり濡れる程度に散
布する。収穫前には、０．５％程度の液肥を株元に施肥
する。In the case of vegetables, if the amino acid fertilizer of the present invention is fertilized in an amount of usually 1 g to 5 g in terms of powder amount per 3.3 m 2 per fertilization, nutritious vegetables will grow and
It has deep leaf color, brightens vegetables, has a photosynthesis promoting effect, and has a long shelf life after harvest. For example, for vegetables, about 0.2% of liquid fertilizer can be used at the time of germination, planting of seedlings, replanting, etc. Disperse so that it is moist. In addition, about 0.1% of liquid fertilizer can be used for foliar spraying and root spraying after transplantation, and once a week, the surface of leaves is wetted or the plant is moistened wetly. Sprinkle on. Before harvesting, about 0.5% liquid fertilizer is applied to the plant base.
【００１２】トマト、キュウリ、ナス等の果菜類には、
通常０．５％〜１％程度の水溶液を使用することが好ま
しい。これら果菜類には、通常収穫約一ヶ月前に葉面全
面に散布する。これにより、葉色の濃緑色化及び果実の
旨味の増進効果を奏することができる。[0012] Tomatoes, cucumbers, eggplants and other fruit vegetables,
Usually, it is preferable to use an aqueous solution of about 0.5% to 1%. These fruits and vegetables are usually sprayed over the entire leaf surface about one month before harvesting. As a result, the effect of darkening the leaf color and enhancing the umami of the fruit can be achieved.
【００１３】リンゴ、梨等の果実類には、通常１％〜３
％程度の水溶液を使用することが好ましい。これら果実
類には、通常収穫約一ヶ月前に葉面全面に散布する。こ
れにより葉色の濃緑色化及び果実の旨味の増進効果を奏
することができる。For fruits such as apples and pears, usually 1% to 3%
%, It is preferable to use an aqueous solution. These fruits are usually sprayed over the entire leaf surface about one month before harvesting. As a result, it is possible to achieve the effect of darkening the leaf color and enhancing the umami of the fruit.
【００１４】稲作の場合には、苗には１０００倍以下希
釈液、田植え後には２００ｍ２当り５００ｇ程度が好ま
しい。本発明の天然アミノ酸肥料は、本発明の目的を損
なわない範囲であれば、他の肥料とを併用することがで
きる。In the case of rice cultivation, it is preferable that the seedlings have a dilution ratio of 1000 times or less and about 500 g per 200 m 2 after rice planting. The natural amino acid fertilizer of the present invention can be used in combination with other fertilizers as long as the object of the present invention is not impaired.
【００１５】本発明の天然アミノ酸肥料を用いて数種の
葉菜類、果菜類、果実類、稲等の作物について施肥実験
を行った。この結果、いずれも著しい効果が得られた。Using the natural amino acid fertilizer of the present invention, fertilization experiments were conducted on several kinds of leaf vegetables, fruit vegetables, fruits, rice and other crops. As a result, remarkable effects were obtained in all cases.
【００１６】[0016]
【発明の効果】絹タンパク質由来の天然アミノ酸肥料
は、葉菜類、果菜類、果実類、稲等の作物の成長に著し
い効果がもたらされることが実験の結果判明している
が、本発明においては、繭の中のさなぎや糸クズ等も有
効利用することにより大巾なコスト低減を実現すると共
に、廃棄物の減量を通じて環境の保護にも役立つ。As a result of experiments, it has been found that the natural amino acid fertilizer derived from silk protein has a remarkable effect on the growth of crops such as leaf vegetables, fruit vegetables, fruits and rice. By making effective use of pupa and lint in cocoons, the cost can be significantly reduced, and the amount of waste can be reduced to help protect the environment.
Claims (2)
塩酸を加えて加水分解し、これから得られたアミノ酸溶
液を水酸化ナトリウムで中和、濃縮し、この濃縮液を透
析してアミノ酸溶液を分離、抽出し、これを凍結乾燥し
て粉末のシルクパウダーを作製し、他方上記工程で加水
分解されなかったさなぎをすり潰して再度上記工程によ
り酸加水分解し、所定の方法で脱脂、粉末化した後、上
記シルクパウダーと粉末化したさなぎを混合して得られ
た、絹タンパク質由来の天然アミノ酸肥料及びその製造
方法。1. A cocoon is boiled in an equal volume of hot water, hydrolyzed by adding a specified amount of hydrochloric acid, the resulting amino acid solution is neutralized with sodium hydroxide and concentrated, and the concentrated solution is dialyzed. Then, the amino acid solution is separated and extracted, and this is freeze-dried to prepare a powdered silk powder, while the pupa that has not been hydrolyzed in the above step is ground and acid-hydrolyzed again in the above step, and then the predetermined method is used. A natural amino acid fertilizer derived from silk protein, obtained by mixing the silk powder with powdered pupa after degreasing and powdering, and a method for producing the same.
ノ酸肥料において、シルクパウダーの平均分子量を３０
０以下とした、絹タンパク質由来の天然アミノ酸肥料及
びその製造方法。2. The natural amino acid fertilizer obtained by the method of claim 1, wherein the silk powder has an average molecular weight of 30.
A natural amino acid fertilizer derived from silk protein, which is 0 or less, and a method for producing the same.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001310730A JP2003073186A (en) | 2001-08-31 | 2001-08-31 | Natural amino acid fertilizer derived from silk protein and its production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001310730A JP2003073186A (en) | 2001-08-31 | 2001-08-31 | Natural amino acid fertilizer derived from silk protein and its production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2003073186A true JP2003073186A (en) | 2003-03-12 |
Family
ID=19129659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001310730A Pending JP2003073186A (en) | 2001-08-31 | 2001-08-31 | Natural amino acid fertilizer derived from silk protein and its production method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2003073186A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020033450A (en) * | 2002-04-08 | 2002-05-06 | 장영수 | Bio-stimulating fertilizer using silkworm pupae containing free amino acids. |
| JP2010254596A (en) * | 2009-04-22 | 2010-11-11 | Yamaka Seiren:Kk | Plant additive |
| CN102731203A (en) * | 2012-06-13 | 2012-10-17 | 吉林加一土产有限公司 | Soil conditioner for old ginseng fields |
| CN104402582A (en) * | 2014-10-30 | 2015-03-11 | 成都新柯力化工科技有限公司 | Fertilizer specially used for increasing fragrance of cucumber and application |
| CN107628876A (en) * | 2017-10-26 | 2018-01-26 | 安徽耘泰农业发展有限公司 | A kind of composite fertilizer's preparation method for improving rice yield |
| CN107673846A (en) * | 2017-10-26 | 2018-02-09 | 安徽耘泰农业发展有限公司 | A kind of high usage Rice composite fertilizer preparation method |
-
2001
- 2001-08-31 JP JP2001310730A patent/JP2003073186A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020033450A (en) * | 2002-04-08 | 2002-05-06 | 장영수 | Bio-stimulating fertilizer using silkworm pupae containing free amino acids. |
| JP2010254596A (en) * | 2009-04-22 | 2010-11-11 | Yamaka Seiren:Kk | Plant additive |
| CN102731203A (en) * | 2012-06-13 | 2012-10-17 | 吉林加一土产有限公司 | Soil conditioner for old ginseng fields |
| CN104402582A (en) * | 2014-10-30 | 2015-03-11 | 成都新柯力化工科技有限公司 | Fertilizer specially used for increasing fragrance of cucumber and application |
| CN107628876A (en) * | 2017-10-26 | 2018-01-26 | 安徽耘泰农业发展有限公司 | A kind of composite fertilizer's preparation method for improving rice yield |
| CN107673846A (en) * | 2017-10-26 | 2018-02-09 | 安徽耘泰农业发展有限公司 | A kind of high usage Rice composite fertilizer preparation method |
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