JP2002522009A - Ｓｔｅ−２０関連蛋白質キナーゼ - Google Patents
Ｓｔｅ−２０関連蛋白質キナーゼInfo
- Publication number
- JP2002522009A JP2002522009A JP2000543584A JP2000543584A JP2002522009A JP 2002522009 A JP2002522009 A JP 2002522009A JP 2000543584 A JP2000543584 A JP 2000543584A JP 2000543584 A JP2000543584 A JP 2000543584A JP 2002522009 A JP2002522009 A JP 2002522009A
- Authority
- JP
- Japan
- Prior art keywords
- seq
- positions
- kinase
- sequence
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000001253 Protein Kinase Human genes 0.000 title description 51
- 108060006633 protein kinase Proteins 0.000 title description 51
- 108091000080 Phosphotransferase Proteins 0.000 claims abstract description 365
- 102000020233 phosphotransferase Human genes 0.000 claims abstract description 358
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 215
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 208
- 229920001184 polypeptide Polymers 0.000 claims abstract description 202
- 238000000034 method Methods 0.000 claims abstract description 148
- 102100026287 Misshapen-like kinase 1 Human genes 0.000 claims abstract description 91
- 101000987297 Homo sapiens Serine/threonine-protein kinase PAK 4 Proteins 0.000 claims abstract description 83
- 101000987295 Homo sapiens Serine/threonine-protein kinase PAK 5 Proteins 0.000 claims abstract description 78
- 102100027940 Serine/threonine-protein kinase PAK 4 Human genes 0.000 claims abstract description 70
- 101000983111 Homo sapiens Serine/threonine-protein kinase PAK 6 Proteins 0.000 claims abstract description 65
- 102100026840 Serine/threonine-protein kinase PAK 6 Human genes 0.000 claims abstract description 63
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 63
- 201000010099 disease Diseases 0.000 claims abstract description 51
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 37
- 150000001413 amino acids Chemical group 0.000 claims description 339
- 241000282414 Homo sapiens Species 0.000 claims description 253
- 108090000623 proteins and genes Proteins 0.000 claims description 176
- 210000004027 cell Anatomy 0.000 claims description 161
- 125000000539 amino acid group Chemical group 0.000 claims description 140
- 102000004169 proteins and genes Human genes 0.000 claims description 117
- 150000007523 nucleic acids Chemical class 0.000 claims description 114
- 102000039446 nucleic acids Human genes 0.000 claims description 94
- 108020004707 nucleic acids Proteins 0.000 claims description 94
- 210000004899 c-terminal region Anatomy 0.000 claims description 87
- 230000027455 binding Effects 0.000 claims description 80
- 239000002773 nucleotide Substances 0.000 claims description 59
- 125000003729 nucleotide group Chemical group 0.000 claims description 59
- 230000000694 effects Effects 0.000 claims description 58
- 239000000523 sample Substances 0.000 claims description 58
- 125000006850 spacer group Chemical group 0.000 claims description 48
- 239000012634 fragment Substances 0.000 claims description 47
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 40
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 37
- 239000013598 vector Substances 0.000 claims description 32
- 230000003197 catalytic effect Effects 0.000 claims description 28
- 230000014509 gene expression Effects 0.000 claims description 28
- 206010028980 Neoplasm Diseases 0.000 claims description 27
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 27
- 239000002853 nucleic acid probe Substances 0.000 claims description 27
- 239000000126 substance Substances 0.000 claims description 25
- 238000009396 hybridization Methods 0.000 claims description 23
- 230000003993 interaction Effects 0.000 claims description 22
- 230000000295 complement effect Effects 0.000 claims description 21
- 201000011510 cancer Diseases 0.000 claims description 19
- 238000012360 testing method Methods 0.000 claims description 19
- 230000035897 transcription Effects 0.000 claims description 15
- 238000013518 transcription Methods 0.000 claims description 15
- 210000000056 organ Anatomy 0.000 claims description 13
- 241000124008 Mammalia Species 0.000 claims description 12
- 238000003556 assay Methods 0.000 claims description 12
- 208000035475 disorder Diseases 0.000 claims description 12
- 210000004408 hybridoma Anatomy 0.000 claims description 12
- 208000006011 Stroke Diseases 0.000 claims description 10
- 230000008859 change Effects 0.000 claims description 10
- 230000009870 specific binding Effects 0.000 claims description 10
- 208000024172 Cardiovascular disease Diseases 0.000 claims description 9
- 208000010125 myocardial infarction Diseases 0.000 claims description 9
- 208000015122 neurodegenerative disease Diseases 0.000 claims description 9
- 230000036542 oxidative stress Effects 0.000 claims description 9
- 208000001647 Renal Insufficiency Diseases 0.000 claims description 8
- 201000006370 kidney failure Diseases 0.000 claims description 8
- 238000002054 transplantation Methods 0.000 claims description 8
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 7
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 7
- 238000000338 in vitro Methods 0.000 claims description 7
- 229940043355 kinase inhibitor Drugs 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 239000003757 phosphotransferase inhibitor Substances 0.000 claims description 2
- 238000011282 treatment Methods 0.000 abstract description 14
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 229940024606 amino acid Drugs 0.000 description 340
- 235000001014 amino acid Nutrition 0.000 description 339
- 235000018102 proteins Nutrition 0.000 description 112
- 125000003275 alpha amino acid group Chemical group 0.000 description 85
- 101000573441 Homo sapiens Misshapen-like kinase 1 Proteins 0.000 description 78
- 239000002299 complementary DNA Substances 0.000 description 68
- 101001059454 Homo sapiens Serine/threonine-protein kinase MARK2 Proteins 0.000 description 52
- 102100028904 Serine/threonine-protein kinase MARK2 Human genes 0.000 description 52
- 230000006870 function Effects 0.000 description 48
- 101000628647 Homo sapiens Serine/threonine-protein kinase 24 Proteins 0.000 description 47
- 102100026764 Serine/threonine-protein kinase 24 Human genes 0.000 description 46
- 241000699666 Mus <mouse, genus> Species 0.000 description 45
- 230000000875 corresponding effect Effects 0.000 description 42
- 241000244206 Nematoda Species 0.000 description 38
- 230000037361 pathway Effects 0.000 description 37
- 230000036961 partial effect Effects 0.000 description 31
- 102100026737 Serine/threonine-protein kinase 25 Human genes 0.000 description 30
- 101100311456 Caenorhabditis elegans kin-18 gene Proteins 0.000 description 29
- 230000004913 activation Effects 0.000 description 29
- 150000001875 compounds Chemical class 0.000 description 29
- 101710183953 Serine/threonine-protein kinase 25 Proteins 0.000 description 28
- 102100030571 STE20-like serine/threonine-protein kinase Human genes 0.000 description 27
- 102100028192 Mitogen-activated protein kinase kinase kinase kinase 2 Human genes 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 25
- 101710144533 Mitogen-activated protein kinase kinase kinase kinase 2 Proteins 0.000 description 25
- 108700026244 Open Reading Frames Proteins 0.000 description 24
- 101710157230 STE20-like serine/threonine-protein kinase Proteins 0.000 description 24
- 230000001105 regulatory effect Effects 0.000 description 24
- 230000003834 intracellular effect Effects 0.000 description 23
- 238000006366 phosphorylation reaction Methods 0.000 description 23
- 101100401589 Caenorhabditis elegans mig-15 gene Proteins 0.000 description 22
- 101001059982 Homo sapiens Mitogen-activated protein kinase kinase kinase kinase 5 Proteins 0.000 description 21
- 239000003153 chemical reaction reagent Substances 0.000 description 21
- 230000019491 signal transduction Effects 0.000 description 21
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 20
- 108020004705 Codon Proteins 0.000 description 20
- 102100028195 Mitogen-activated protein kinase kinase kinase kinase 5 Human genes 0.000 description 20
- 108010076504 Protein Sorting Signals Proteins 0.000 description 20
- 210000001519 tissue Anatomy 0.000 description 20
- 108010014632 NF-kappa B kinase Proteins 0.000 description 19
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 18
- 229930182817 methionine Natural products 0.000 description 18
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 17
- 108091036066 Three prime untranslated region Proteins 0.000 description 17
- 230000011664 signaling Effects 0.000 description 17
- 238000003277 amino acid sequence analysis Methods 0.000 description 16
- 230000001419 dependent effect Effects 0.000 description 16
- 102000030938 small GTPase Human genes 0.000 description 16
- 230000014621 translational initiation Effects 0.000 description 16
- 238000004422 calculation algorithm Methods 0.000 description 15
- 239000000284 extract Substances 0.000 description 15
- 230000000977 initiatory effect Effects 0.000 description 15
- 102100028194 Mitogen-activated protein kinase kinase kinase kinase 4 Human genes 0.000 description 14
- 108091081024 Start codon Proteins 0.000 description 14
- 230000002159 abnormal effect Effects 0.000 description 14
- 230000001404 mediated effect Effects 0.000 description 14
- 230000026731 phosphorylation Effects 0.000 description 14
- 230000004044 response Effects 0.000 description 14
- 108091035707 Consensus sequence Proteins 0.000 description 13
- 102000000395 SH3 domains Human genes 0.000 description 13
- 108050008861 SH3 domains Proteins 0.000 description 13
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 13
- 238000003780 insertion Methods 0.000 description 13
- 230000037431 insertion Effects 0.000 description 13
- 108090000925 TNF receptor-associated factor 2 Proteins 0.000 description 12
- 102100034779 TRAF family member-associated NF-kappa-B activator Human genes 0.000 description 12
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 12
- 238000001514 detection method Methods 0.000 description 12
- 102000005962 receptors Human genes 0.000 description 12
- 108020003175 receptors Proteins 0.000 description 12
- 108010055717 JNK Mitogen-Activated Protein Kinases Proteins 0.000 description 11
- 102000019145 JUN kinase activity proteins Human genes 0.000 description 11
- 101710144518 Mitogen-activated protein kinase kinase kinase kinase 4 Proteins 0.000 description 11
- 101100311275 Mus musculus Stk10 gene Proteins 0.000 description 11
- 102100027609 Rho-related GTP-binding protein RhoD Human genes 0.000 description 11
- 238000007792 addition Methods 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 11
- 102000050788 human PAK4 Human genes 0.000 description 11
- 102000054042 human PAK5 Human genes 0.000 description 11
- 239000012528 membrane Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 10
- 101800001318 Capsid protein VP4 Proteins 0.000 description 10
- 108091006027 G proteins Proteins 0.000 description 10
- 102000030782 GTP binding Human genes 0.000 description 10
- 108091000058 GTP-Binding Proteins 0.000 description 10
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 10
- 108010067218 Guanine Nucleotide Exchange Factors Proteins 0.000 description 10
- 102000016285 Guanine Nucleotide Exchange Factors Human genes 0.000 description 10
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 10
- 230000001684 chronic effect Effects 0.000 description 10
- 239000003102 growth factor Substances 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 230000007246 mechanism Effects 0.000 description 10
- 108091006026 monomeric small GTPases Proteins 0.000 description 10
- 239000000758 substrate Substances 0.000 description 10
- -1 tyrosine amino acids Chemical class 0.000 description 10
- 238000011144 upstream manufacturing Methods 0.000 description 10
- 208000023275 Autoimmune disease Diseases 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 101000628693 Homo sapiens Serine/threonine-protein kinase 25 Proteins 0.000 description 9
- 101001022129 Homo sapiens Tyrosine-protein kinase Fyn Proteins 0.000 description 9
- 241000700159 Rattus Species 0.000 description 9
- 210000001744 T-lymphocyte Anatomy 0.000 description 9
- 238000010367 cloning Methods 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 238000011160 research Methods 0.000 description 9
- 206010039073 rheumatoid arthritis Diseases 0.000 description 9
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 8
- 102000004127 Cytokines Human genes 0.000 description 8
- 108090000695 Cytokines Proteins 0.000 description 8
- 101000701497 Homo sapiens STE20/SPS1-related proline-alanine-rich protein kinase Proteins 0.000 description 8
- 101000987315 Homo sapiens Serine/threonine-protein kinase PAK 3 Proteins 0.000 description 8
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 8
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 8
- 102000043136 MAP kinase family Human genes 0.000 description 8
- 108091054455 MAP kinase family Proteins 0.000 description 8
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 108010051348 cdc42 GTP-Binding Protein Proteins 0.000 description 8
- 239000003814 drug Substances 0.000 description 8
- 210000004962 mammalian cell Anatomy 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000035772 mutation Effects 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 8
- 230000001225 therapeutic effect Effects 0.000 description 8
- 230000005030 transcription termination Effects 0.000 description 8
- 102000011068 Cdc42 Human genes 0.000 description 7
- 102000007665 Extracellular Signal-Regulated MAP Kinases Human genes 0.000 description 7
- 108010007457 Extracellular Signal-Regulated MAP Kinases Proteins 0.000 description 7
- 101000831887 Homo sapiens STE20-related kinase adapter protein alpha Proteins 0.000 description 7
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 7
- 102000042887 STE20 family Human genes 0.000 description 7
- 108091082301 STE20 family Proteins 0.000 description 7
- 102100024171 STE20-related kinase adapter protein alpha Human genes 0.000 description 7
- 101150073911 STK gene Proteins 0.000 description 7
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 7
- 239000004473 Threonine Substances 0.000 description 7
- 102000013515 cdc42 GTP-Binding Protein Human genes 0.000 description 7
- 238000012217 deletion Methods 0.000 description 7
- 230000037430 deletion Effects 0.000 description 7
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 7
- JYGFTBXVXVMTGB-UHFFFAOYSA-N indolin-2-one Chemical class C1=CC=C2NC(=O)CC2=C1 JYGFTBXVXVMTGB-UHFFFAOYSA-N 0.000 description 7
- 108020004999 messenger RNA Proteins 0.000 description 7
- 102000006271 p21-Activated Kinases Human genes 0.000 description 7
- 108010058266 p21-Activated Kinases Proteins 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000035882 stress Effects 0.000 description 7
- 108050001278 Cdc42 Proteins 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 6
- 102100023274 Dual specificity mitogen-activated protein kinase kinase 4 Human genes 0.000 description 6
- 241000206602 Eukaryota Species 0.000 description 6
- 101001066435 Homo sapiens Hepatocyte growth factor-like protein Proteins 0.000 description 6
- 101000880431 Homo sapiens Serine/threonine-protein kinase 4 Proteins 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 201000004681 Psoriasis Diseases 0.000 description 6
- 102100030491 STE20/SPS1-related proline-alanine-rich protein kinase Human genes 0.000 description 6
- 102100037629 Serine/threonine-protein kinase 4 Human genes 0.000 description 6
- 230000005856 abnormality Effects 0.000 description 6
- 239000000427 antigen Substances 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 230000006907 apoptotic process Effects 0.000 description 6
- 210000003719 b-lymphocyte Anatomy 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 210000003527 eukaryotic cell Anatomy 0.000 description 6
- 102000057823 human SLK Human genes 0.000 description 6
- 238000003018 immunoassay Methods 0.000 description 6
- 239000003112 inhibitor Substances 0.000 description 6
- 230000004770 neurodegeneration Effects 0.000 description 6
- 201000008482 osteoarthritis Diseases 0.000 description 6
- 102000002574 p38 Mitogen-Activated Protein Kinases Human genes 0.000 description 6
- 108010068338 p38 Mitogen-Activated Protein Kinases Proteins 0.000 description 6
- 102000016914 ras Proteins Human genes 0.000 description 6
- 108060007624 small GTPase Proteins 0.000 description 6
- 150000003384 small molecules Chemical class 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 210000001541 thymus gland Anatomy 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 241000219194 Arabidopsis Species 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 102100037912 Cyclin-dependent kinase 11A Human genes 0.000 description 5
- 102100031480 Dual specificity mitogen-activated protein kinase kinase 1 Human genes 0.000 description 5
- 101710146526 Dual specificity mitogen-activated protein kinase kinase 1 Proteins 0.000 description 5
- 101710146518 Dual specificity mitogen-activated protein kinase kinase 4 Proteins 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 101000738403 Homo sapiens Cyclin-dependent kinase 11A Proteins 0.000 description 5
- 101000927796 Homo sapiens Rho guanine nucleotide exchange factor 7 Proteins 0.000 description 5
- 101000987310 Homo sapiens Serine/threonine-protein kinase PAK 2 Proteins 0.000 description 5
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 5
- 229940124647 MEK inhibitor Drugs 0.000 description 5
- 208000029082 Pelvic Inflammatory Disease Diseases 0.000 description 5
- 102100027939 Serine/threonine-protein kinase PAK 2 Human genes 0.000 description 5
- 102100027911 Serine/threonine-protein kinase PAK 3 Human genes 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 208000006673 asthma Diseases 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 239000000539 dimer Substances 0.000 description 5
- 208000037765 diseases and disorders Diseases 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000004660 morphological change Effects 0.000 description 5
- 201000006417 multiple sclerosis Diseases 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 230000008488 polyadenylation Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 210000001236 prokaryotic cell Anatomy 0.000 description 5
- 108010014186 ras Proteins Proteins 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 206010039083 rhinitis Diseases 0.000 description 5
- 230000008093 supporting effect Effects 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108020005345 3' Untranslated Regions Proteins 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 101001059984 Homo sapiens Mitogen-activated protein kinase kinase kinase kinase 4 Proteins 0.000 description 4
- 101001129076 Homo sapiens Serine/threonine-protein kinase N1 Proteins 0.000 description 4
- 101000987317 Homo sapiens Serine/threonine-protein kinase PAK 1 Proteins 0.000 description 4
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 4
- 239000005089 Luciferase Substances 0.000 description 4
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 4
- 102100033479 RAF proto-oncogene serine/threonine-protein kinase Human genes 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 4
- 230000001594 aberrant effect Effects 0.000 description 4
- 230000003213 activating effect Effects 0.000 description 4
- 239000012190 activator Substances 0.000 description 4
- 239000000556 agonist Substances 0.000 description 4
- 239000005557 antagonist Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 4
- 239000000306 component Substances 0.000 description 4
- 230000001086 cytosolic effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 102000034345 heterotrimeric G proteins Human genes 0.000 description 4
- 108091006093 heterotrimeric G proteins Proteins 0.000 description 4
- 102000044476 human PAK1 Human genes 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 238000002649 immunization Methods 0.000 description 4
- 230000002757 inflammatory effect Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 4
- 230000011278 mitosis Effects 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 230000001590 oxidative effect Effects 0.000 description 4
- 230000000865 phosphorylative effect Effects 0.000 description 4
- 239000013600 plasmid vector Substances 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 125000002294 quinazolinyl group Chemical class N1=C(N=CC2=CC=CC=C12)* 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- 239000013603 viral vector Substances 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 108700028369 Alleles Proteins 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 3
- 201000001320 Atherosclerosis Diseases 0.000 description 3
- 241000701822 Bovine papillomavirus Species 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 208000005623 Carcinogenesis Diseases 0.000 description 3
- 208000031229 Cardiomyopathies Diseases 0.000 description 3
- 102000014914 Carrier Proteins Human genes 0.000 description 3
- 208000011231 Crohn disease Diseases 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 238000009007 Diagnostic Kit Methods 0.000 description 3
- 108010009202 Growth Factor Receptors Proteins 0.000 description 3
- 102000009465 Growth Factor Receptors Human genes 0.000 description 3
- 101001059990 Homo sapiens Mitogen-activated protein kinase kinase kinase kinase 2 Proteins 0.000 description 3
- 101000652133 Homo sapiens STE20-like serine/threonine-protein kinase Proteins 0.000 description 3
- 101000838579 Homo sapiens Serine/threonine-protein kinase TAO1 Proteins 0.000 description 3
- 101000838578 Homo sapiens Serine/threonine-protein kinase TAO2 Proteins 0.000 description 3
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 3
- 108060001084 Luciferase Proteins 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 3
- 108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 3
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 description 3
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 description 3
- 101150101372 RAF1 gene Proteins 0.000 description 3
- 102000001332 SRC Human genes 0.000 description 3
- 101710183160 Serine/threonine-protein kinase PLK1 Proteins 0.000 description 3
- 102100028948 Serine/threonine-protein kinase TAO1 Human genes 0.000 description 3
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 3
- 108091023045 Untranslated Region Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 230000001270 agonistic effect Effects 0.000 description 3
- 230000003042 antagnostic effect Effects 0.000 description 3
- 229940111121 antirheumatic drug quinolines Drugs 0.000 description 3
- 230000010455 autoregulation Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 230000036952 cancer formation Effects 0.000 description 3
- 231100000504 carcinogenesis Toxicity 0.000 description 3
- 108020001778 catalytic domains Proteins 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 230000036755 cellular response Effects 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 108010060348 citron-kinase Proteins 0.000 description 3
- 238000012761 co-transfection Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 210000004292 cytoskeleton Anatomy 0.000 description 3
- 210000004443 dendritic cell Anatomy 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 230000007783 downstream signaling Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 210000002216 heart Anatomy 0.000 description 3
- 210000003630 histaminocyte Anatomy 0.000 description 3
- 102000044481 human PAK3 Human genes 0.000 description 3
- 208000026278 immune system disease Diseases 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 238000011005 laboratory method Methods 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 239000003226 mitogen Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000008506 pathogenesis Effects 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 150000003147 proline derivatives Chemical class 0.000 description 3
- 230000004850 protein–protein interaction Effects 0.000 description 3
- 150000003248 quinolines Chemical class 0.000 description 3
- 150000003252 quinoxalines Chemical class 0.000 description 3
- 238000003127 radioimmunoassay Methods 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000009221 stress response pathway Effects 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- CXWXQJXEFPUFDZ-UHFFFAOYSA-N tetralin Chemical compound C1=CC=C2CCCCC2=C1 CXWXQJXEFPUFDZ-UHFFFAOYSA-N 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- 229940121358 tyrosine kinase inhibitor Drugs 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical group NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 2
- 229920000178 Acrylic resin Polymers 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 102100034452 Alternative prion protein Human genes 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 101000755496 Canis lupus familiaris Transforming protein RhoA Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 241000700198 Cavia Species 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 240000004307 Citrus medica Species 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 102000010170 Death domains Human genes 0.000 description 2
- 108050001718 Death domains Proteins 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108700030733 Drosophila Pak Proteins 0.000 description 2
- 241000255601 Drosophila melanogaster Species 0.000 description 2
- 102100023275 Dual specificity mitogen-activated protein kinase kinase 3 Human genes 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000701959 Escherichia virus Lambda Species 0.000 description 2
- 101150064015 FAS gene Proteins 0.000 description 2
- 102100026693 FAS-associated death domain protein Human genes 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241001272567 Hominoidea Species 0.000 description 2
- 101001115394 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 3 Proteins 0.000 description 2
- 101000979342 Homo sapiens Nuclear factor NF-kappa-B p105 subunit Proteins 0.000 description 2
- 101000669917 Homo sapiens Rho-associated protein kinase 1 Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010023230 Joint stiffness Diseases 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 102000019149 MAP kinase activity proteins Human genes 0.000 description 2
- 108040008097 MAP kinase activity proteins Proteins 0.000 description 2
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- 206010058799 Mitochondrial encephalomyopathy Diseases 0.000 description 2
- 108700027649 Mitogen-Activated Protein Kinase 3 Proteins 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 102100023050 Nuclear factor NF-kappa-B p105 subunit Human genes 0.000 description 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
- 108030005449 Polo kinases Proteins 0.000 description 2
- 108091000054 Prion Proteins 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
- 101100244097 Rattus norvegicus Pkn1 gene Proteins 0.000 description 2
- 102000042463 Rho family Human genes 0.000 description 2
- 108091078243 Rho family Proteins 0.000 description 2
- 102100039313 Rho-associated protein kinase 1 Human genes 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
- 108060006706 SRC Proteins 0.000 description 2
- 208000034189 Sclerosis Diseases 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 230000006044 T cell activation Effects 0.000 description 2
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 2
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 2
- 102100040247 Tumor necrosis factor Human genes 0.000 description 2
- 101710187743 Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 description 2
- 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 241000269370 Xenopus <genus> Species 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000033115 angiogenesis Effects 0.000 description 2
- 230000002788 anti-peptide Effects 0.000 description 2
- 239000000074 antisense oligonucleotide Substances 0.000 description 2
- 238000012230 antisense oligonucleotides Methods 0.000 description 2
- 230000001640 apoptogenic effect Effects 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000035578 autophosphorylation Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000012292 cell migration Effects 0.000 description 2
- 230000010307 cell transformation Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000012433 dorsal closure Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 231100000221 frame shift mutation induction Toxicity 0.000 description 2
- 230000037433 frameshift Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000002414 glycolytic effect Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000003394 haemopoietic effect Effects 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 108010002838 hematopoietic progenitor kinase 1 Proteins 0.000 description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 2
- 102000048890 human MAP4K2 Human genes 0.000 description 2
- 102000047858 human MAP4K4 Human genes 0.000 description 2
- 102000046188 human STK39 Human genes 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 239000003018 immunosuppressive agent Substances 0.000 description 2
- 238000012623 in vivo measurement Methods 0.000 description 2
- 238000012625 in-situ measurement Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 125000002950 monocyclic group Chemical group 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000003757 neuroblast Anatomy 0.000 description 2
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 2
- 239000007800 oxidant agent Substances 0.000 description 2
- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000034190 positive regulation of NF-kappaB transcription factor activity Effects 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000013615 primer Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 101150090168 rab8A gene Proteins 0.000 description 2
- 239000003642 reactive oxygen metabolite Substances 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000007781 signaling event Effects 0.000 description 2
- 125000005504 styryl group Chemical group 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 210000001550 testis Anatomy 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011426 transformation method Methods 0.000 description 2
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 2
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 230000009750 upstream signaling Effects 0.000 description 2
- 230000028973 vesicle-mediated transport Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- RYCSNJCJOVQGBC-UHFFFAOYSA-N 3-(1-cyclopropyl-2h-pyridin-4-yl)-1h-quinolin-2-one Chemical class O=C1NC2=CC=CC=C2C=C1C(C=C1)=CCN1C1CC1 RYCSNJCJOVQGBC-UHFFFAOYSA-N 0.000 description 1
- ROKOFZNQCIIJMI-UHFFFAOYSA-N 4-(4-fluorophenyl)-1-cycloropropylmethyl-5-(4-pyridyl)-imidazole Chemical compound C1=CC(F)=CC=C1C1=C(C=2C=CN=CC=2)N(CC2CC2)C=N1 ROKOFZNQCIIJMI-UHFFFAOYSA-N 0.000 description 1
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
- 241000203809 Actinomycetales Species 0.000 description 1
- 208000009304 Acute Kidney Injury Diseases 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102100033972 Amyloid protein-binding protein 2 Human genes 0.000 description 1
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 230000020955 B cell costimulation Effects 0.000 description 1
- WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 101900315840 Bacillus subtilis Alpha-amylase Proteins 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 101150013553 CD40 gene Proteins 0.000 description 1
- 108091007914 CDKs Proteins 0.000 description 1
- 101150020019 CLA4 gene Proteins 0.000 description 1
- 108010026870 Calcium-Calmodulin-Dependent Protein Kinases Proteins 0.000 description 1
- 102000019025 Calcium-Calmodulin-Dependent Protein Kinases Human genes 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 102000011727 Caspases Human genes 0.000 description 1
- 108010076667 Caspases Proteins 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102100034744 Cell division cycle 7-related protein kinase Human genes 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 235000021538 Chard Nutrition 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102100023033 Cyclic AMP-dependent transcription factor ATF-2 Human genes 0.000 description 1
- 102000003903 Cyclin-dependent kinases Human genes 0.000 description 1
- 108090000266 Cyclin-dependent kinases Proteins 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 101000785279 Dictyostelium discoideum Calcium-transporting ATPase PAT1 Proteins 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 108700010895 Drosophila ADH Proteins 0.000 description 1
- 101100028477 Drosophila melanogaster Pak gene Proteins 0.000 description 1
- 101100015729 Drosophila melanogaster drk gene Proteins 0.000 description 1
- 101100175356 Drosophila melanogaster gek gene Proteins 0.000 description 1
- 108700027988 Drosophila msn Proteins 0.000 description 1
- 108700007864 Drosophila ninaC Proteins 0.000 description 1
- 101710146524 Dual specificity mitogen-activated protein kinase kinase 5 Proteins 0.000 description 1
- 102100023272 Dual specificity mitogen-activated protein kinase kinase 5 Human genes 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108010077716 Fas-Associated Death Domain Protein Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 1
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 1
- 101150082479 GAL gene Proteins 0.000 description 1
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
- 102000000312 GTP-Binding Protein beta Subunits Human genes 0.000 description 1
- 108010080615 GTP-Binding Protein beta Subunits Proteins 0.000 description 1
- 108091006109 GTPases Proteins 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Chemical group OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 101000684275 Homo sapiens ADP-ribosylation factor 3 Proteins 0.000 description 1
- 101000779309 Homo sapiens Amyloid protein-binding protein 2 Proteins 0.000 description 1
- 101000945740 Homo sapiens Cell division cycle 7-related protein kinase Proteins 0.000 description 1
- 101000974934 Homo sapiens Cyclic AMP-dependent transcription factor ATF-2 Proteins 0.000 description 1
- 101001115395 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 4 Proteins 0.000 description 1
- 101000911074 Homo sapiens FAS-associated death domain protein Proteins 0.000 description 1
- 101000997829 Homo sapiens Glial cell line-derived neurotrophic factor Proteins 0.000 description 1
- 101000871017 Homo sapiens Growth factor receptor-bound protein 2 Proteins 0.000 description 1
- 101100182846 Homo sapiens MAP4K4 gene Proteins 0.000 description 1
- 101000692455 Homo sapiens Platelet-derived growth factor receptor beta Proteins 0.000 description 1
- 101000713296 Homo sapiens Proton-coupled amino acid transporter 1 Proteins 0.000 description 1
- 101001130437 Homo sapiens Ras-related protein Rap-2b Proteins 0.000 description 1
- 101100204244 Homo sapiens STK24 gene Proteins 0.000 description 1
- 101000648174 Homo sapiens Serine/threonine-protein kinase 10 Proteins 0.000 description 1
- 101000880439 Homo sapiens Serine/threonine-protein kinase 3 Proteins 0.000 description 1
- 101000662997 Homo sapiens TRAF2 and NCK-interacting protein kinase Proteins 0.000 description 1
- 101000938391 Homo sapiens Transmembrane protein Proteins 0.000 description 1
- 101001064119 Homo sapiens Truncated surface protein Proteins 0.000 description 1
- 101001064124 Homo sapiens Truncated surface protein Proteins 0.000 description 1
- 101000850748 Homo sapiens Tumor necrosis factor receptor type 1-associated DEATH domain protein Proteins 0.000 description 1
- 108010084873 Human Immunodeficiency Virus nef Gene Products Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical group OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 208000006136 Leigh Disease Diseases 0.000 description 1
- 208000017507 Leigh syndrome Diseases 0.000 description 1
- 102000001291 MAP Kinase Kinase Kinase Human genes 0.000 description 1
- 108060006687 MAP kinase kinase kinase Proteins 0.000 description 1
- 241001599018 Melanogaster Species 0.000 description 1
- 208000036626 Mental retardation Diseases 0.000 description 1
- 102000003792 Metallothionein Human genes 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 102000046795 Mitogen-Activated Protein Kinase 3 Human genes 0.000 description 1
- 108700027650 Mitogen-Activated Protein Kinase 7 Proteins 0.000 description 1
- 108700027648 Mitogen-Activated Protein Kinase 8 Proteins 0.000 description 1
- 108700027653 Mitogen-Activated Protein Kinase 9 Proteins 0.000 description 1
- 102100024192 Mitogen-activated protein kinase 3 Human genes 0.000 description 1
- 102100037805 Mitogen-activated protein kinase 7 Human genes 0.000 description 1
- 102100037808 Mitogen-activated protein kinase 8 Human genes 0.000 description 1
- 102100033115 Mitogen-activated protein kinase kinase kinase 1 Human genes 0.000 description 1
- 101710164423 Mitogen-activated protein kinase kinase kinase 1 Proteins 0.000 description 1
- 101000969137 Mus musculus Metallothionein-1 Proteins 0.000 description 1
- 101100350375 Mus musculus Rho gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010028289 Muscle atrophy Diseases 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- LTBRACVJRXLQHC-UHFFFAOYSA-N OP(=O)OCC1=CC=CC=C1 Chemical class OP(=O)OCC1=CC=CC=C1 LTBRACVJRXLQHC-UHFFFAOYSA-N 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000007074 Phospholipase C beta Human genes 0.000 description 1
- 108010047834 Phospholipase C beta Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical group OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 101710192141 Protein Nef Proteins 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 102000012515 Protein kinase domains Human genes 0.000 description 1
- 108050002122 Protein kinase domains Proteins 0.000 description 1
- 101150058540 RAC1 gene Proteins 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 101150065817 ROM2 gene Proteins 0.000 description 1
- 102100022122 Ras-related C3 botulinum toxin substrate 1 Human genes 0.000 description 1
- 102100031421 Ras-related protein Rap-2b Human genes 0.000 description 1
- 108090000873 Receptor Protein-Tyrosine Kinases Proteins 0.000 description 1
- 102000004278 Receptor Protein-Tyrosine Kinases Human genes 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 208000033626 Renal failure acute Diseases 0.000 description 1
- 206010063837 Reperfusion injury Diseases 0.000 description 1
- 102000014400 SH2 domains Human genes 0.000 description 1
- 108050003452 SH2 domains Proteins 0.000 description 1
- 108091009674 SH3 domain binding proteins Proteins 0.000 description 1
- 102000033686 SH3 domain binding proteins Human genes 0.000 description 1
- 102100036901 SLC2A4 regulator Human genes 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 101100149535 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SKM1 gene Proteins 0.000 description 1
- 241000235343 Saccharomycetales Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 102100028900 Serine/threonine-protein kinase 10 Human genes 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 206010041067 Small cell lung cancer Diseases 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 230000020385 T cell costimulation Effects 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 102100037671 TRAF2 and NCK-interacting protein kinase Human genes 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 description 1
- 102100033733 Tumor necrosis factor receptor superfamily member 1B Human genes 0.000 description 1
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 1
- 102100033081 Tumor necrosis factor receptor type 1-associated DEATH domain protein Human genes 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 210000001766 X chromosome Anatomy 0.000 description 1
- 101000752225 Xenopus laevis Low density lipoprotein receptor adapter protein 1-A Proteins 0.000 description 1
- 101000729939 Xenopus laevis Serine/threonine-protein kinase PLK1 Proteins 0.000 description 1
- 101100127670 Zea mays LA1 gene Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 201000011040 acute kidney failure Diseases 0.000 description 1
- 208000012998 acute renal failure Diseases 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000007801 affinity label Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000006229 amino acid addition Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 229940045988 antineoplastic drug protein kinase inhibitors Drugs 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000005775 apoptotic pathway Effects 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 125000002619 bicyclic group Chemical group 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229950004398 broxuridine Drugs 0.000 description 1
- 201000011529 cardiovascular cancer Diseases 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 230000004637 cellular stress Effects 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000014107 chromosome localization Effects 0.000 description 1
- 230000003920 cognitive function Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 229960001334 corticosteroids Drugs 0.000 description 1
- 230000004940 costimulation Effects 0.000 description 1
- 108091008034 costimulatory receptors Proteins 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 102000003675 cytokine receptors Human genes 0.000 description 1
- 108010057085 cytokine receptors Proteins 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000003436 cytoskeletal effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000000586 desensitisation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000001973 epigenetic effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000003176 fibrotic effect Effects 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 210000001650 focal adhesion Anatomy 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 210000001280 germinal center Anatomy 0.000 description 1
- 210000001102 germinal center b cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Chemical group 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 101150098203 grb2 gene Proteins 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 238000005734 heterodimerization reaction Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 102000051937 human MAP4K5 Human genes 0.000 description 1
- 102000047501 human STK3 Human genes 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 229940124589 immunosuppressive drug Drugs 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 1
- 230000006662 intracellular pathway Effects 0.000 description 1
- 230000004068 intracellular signaling Effects 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 101150109249 lacI gene Proteins 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 201000010901 lateral sclerosis Diseases 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000005004 lymphoid follicle Anatomy 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000003519 mature b lymphocyte Anatomy 0.000 description 1
- 210000003593 megakaryocyte Anatomy 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 101150042181 mig-15 gene Proteins 0.000 description 1
- 230000002297 mitogenic effect Effects 0.000 description 1
- 208000005264 motor neuron disease Diseases 0.000 description 1
- 230000020763 muscle atrophy Effects 0.000 description 1
- 201000000585 muscular atrophy Diseases 0.000 description 1
- 210000002686 mushroom body Anatomy 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 230000007971 neurological deficit Effects 0.000 description 1
- 230000014511 neuron projection development Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 201000005528 peripheral nervous system neoplasm Diseases 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 230000010422 pheromone response pathway Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 230000000770 proinflammatory effect Effects 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 239000003909 protein kinase inhibitor Substances 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 125000004076 pyridyl group Chemical class 0.000 description 1
- 101150079601 recA gene Proteins 0.000 description 1
- 210000003370 receptor cell Anatomy 0.000 description 1
- 230000037425 regulation of transcription Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000009712 regulation of translation Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000011506 response to oxidative stress Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000004116 schwann cell Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 150000003346 selenoethers Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 102000034285 signal transducing proteins Human genes 0.000 description 1
- 108091006024 signal transducing proteins Proteins 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 108010087686 src-Family Kinases Proteins 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 210000001768 subcellular fraction Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 210000003412 trans-golgi network Anatomy 0.000 description 1
- 230000005758 transcription activity Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000006276 transfer reaction Methods 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 150000005691 triesters Chemical class 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000005748 tumor development Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 108010002164 tyrosine receptor Proteins 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 235000012711 vitamin K3 Nutrition 0.000 description 1
- 239000011652 vitamin K3 Substances 0.000 description 1
- 229940041603 vitamin k 3 Drugs 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Neurology (AREA)
- Immunology (AREA)
- Neurosurgery (AREA)
- Vascular Medicine (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8178498P | 1998-04-14 | 1998-04-14 | |
| US60/081,784 | 1998-04-14 | ||
| PCT/US1999/008150 WO1999053036A2 (en) | 1998-04-14 | 1999-04-13 | Ste20-related protein kinases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002522009A true JP2002522009A (ja) | 2002-07-23 |
| JP2002522009A5 JP2002522009A5 (enExample) | 2006-05-25 |
Family
ID=22166386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000543584A Withdrawn JP2002522009A (ja) | 1998-04-14 | 1999-04-13 | Ｓｔｅ−２０関連蛋白質キナーゼ |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US6680170B2 (enExample) |
| EP (1) | EP1073723B1 (enExample) |
| JP (1) | JP2002522009A (enExample) |
| AT (1) | ATE302269T1 (enExample) |
| AU (1) | AU3642499A (enExample) |
| CA (1) | CA2369172A1 (enExample) |
| DE (1) | DE69926742T2 (enExample) |
| DK (1) | DK1073723T3 (enExample) |
| ES (1) | ES2248997T3 (enExample) |
| WO (1) | WO1999053036A2 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006507835A (ja) | 2002-11-27 | 2006-03-09 | スージェン・インコーポレーテッド | リン酸特異的ｐａｋ抗体および診断キット |
| JP2012023006A (ja) * | 2010-07-15 | 2012-02-02 | Hyundai Motor Co Ltd | リチウム二次電池用陽極材料及びその製造方法並びにその材料を含むリチウム二次電池 |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7078182B1 (en) * | 1998-04-14 | 2006-07-18 | Board Of Regents, The University Of Texas System | TAO protein kinase polypeptides and methods of use therefor |
| US6013500A (en) | 1998-05-21 | 2000-01-11 | The Trustees Of Columbia University In The City Of New York | PAK4, a novel gene encoding a serine/threonine kinase |
| EP1112368A1 (en) * | 1998-09-10 | 2001-07-04 | Smithkline Beecham Plc | Use of protein kinase kiaa0551 as pharmaceutical |
| CA2362936A1 (en) * | 1999-02-19 | 2000-08-24 | Mcmaster University | A caspase activated protein kinase |
| AU3566200A (en) * | 1999-04-01 | 2000-10-23 | Astrazeneca Uk Limited | Human signal transduction serine/threonine protein kinase |
| US6558903B1 (en) * | 1999-06-30 | 2003-05-06 | Millennium Pharmaceuticals, Inc. | Kinases and uses thereof |
| AU6181000A (en) * | 1999-07-29 | 2001-02-19 | Chugai Research Institute For Molecular Medicine, Inc. | Novel genes encoding protein kinase/protein phosphatase |
| US20030104622A1 (en) * | 1999-09-01 | 2003-06-05 | Robbins Paul D. | Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses |
| US6861239B1 (en) | 1999-09-20 | 2005-03-01 | New York University | Genes and polynucleotides associated with ultraviolet radiation-mediated skin damage and uses thereof |
| WO2001036602A2 (en) * | 1999-11-15 | 2001-05-25 | Pharmacia Italia S.P.A. | Pak5 a member of the p21-activated kinase (pak) protein family, nucleic acids and methods related to the same |
| EP1254214A2 (en) * | 2000-01-25 | 2002-11-06 | Sugen, Inc. | Human protein kinases and protein kinase-like enzymes |
| EP1385992A2 (en) * | 2000-09-20 | 2004-02-04 | Kinetek Pharmaceuticals, Inc. | Cancer associated protein kinases and their uses |
| AU2002243539A1 (en) * | 2001-01-12 | 2002-07-24 | Incyte Genomics, Inc. | Molecules for disease detection and treatment |
| US7265214B2 (en) * | 2001-10-19 | 2007-09-04 | Rigel Pharmaceuticals, Inc. | Germinal center kinase proteins, compositions and methods of use |
| JP2005518198A (ja) | 2001-12-28 | 2005-06-23 | ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク | Ｐａｋ５関連の組成物及び方法 |
| US7439329B2 (en) * | 2002-07-05 | 2008-10-21 | Sugen, Inc. | GEF-H1b: biomarkers, complexes assays and therapeutic uses thereof |
| GB0220770D0 (en) * | 2002-09-06 | 2002-10-16 | Ares Trading Sa | Proteins |
| AU2003296696A1 (en) * | 2002-12-23 | 2004-07-14 | Devgen Nv | Kinase sequences |
| GB2396615A (en) * | 2002-12-23 | 2004-06-30 | Devgen Nv | Kinase sequences useful for developing compounds for the prevention and/or treatment of metabolic diseases |
| US20040242461A1 (en) * | 2003-04-08 | 2004-12-02 | Schneider Michael D. | Modulators of telomere stability |
| DK1653844T3 (da) * | 2003-08-13 | 2013-03-11 | Novartis Vaccines & Diagnostic | Prionspecifikke peptidreagenser |
| US20060035242A1 (en) * | 2004-08-13 | 2006-02-16 | Michelitsch Melissa D | Prion-specific peptide reagents |
| AU2004275876B2 (en) * | 2003-09-25 | 2011-03-31 | Coley Pharmaceutical Gmbh | Nucleic acid-lipophilic conjugates |
| WO2005111234A2 (en) * | 2004-05-12 | 2005-11-24 | Sugen, Inc. | Methods of using zc1 and zc3 kinase substrate phosphorylation as biomarkers |
| NZ587255A (en) * | 2005-01-13 | 2012-03-30 | Novartis Vaccines & Diagnostic | Elisa assays using prion-specific peptide reagents |
| WO2006076683A2 (en) * | 2005-01-13 | 2006-07-20 | Novartis Vaccines And Diagnostics Inc. | Isolation and detection of pathogenic prions |
| US20090099343A1 (en) * | 2005-01-13 | 2009-04-16 | David Peretz | Isolation of pathogenic prions |
| NZ566020A (en) * | 2005-09-09 | 2012-08-31 | Novartis Ag | Prion-specific peptoid reagents |
| WO2009134942A1 (en) * | 2008-04-30 | 2009-11-05 | Novartis Ag. | Assay for pathogenic conformers |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4343940A (en) | 1979-02-13 | 1982-08-10 | Mead Johnson & Company | Anti-tumor quinazoline compounds |
| DE3069468D1 (en) | 1979-12-19 | 1984-11-22 | Nat Res Dev | Quinazoline derivatives, processes for their preparation, compositions containing them and their use as anti-cancer agents |
| US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| DE3611194A1 (de) | 1986-04-04 | 1987-10-08 | Bayer Ag | Cancerostatisches mittel |
| US5217999A (en) | 1987-12-24 | 1993-06-08 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Styryl compounds which inhibit EGF receptor protein tyrosine kinase |
| DE3920029C2 (de) | 1988-06-30 | 1999-05-20 | Clariant Finance Bvi Ltd | Farbstoffe zum Färben von Kunststoffen |
| ATE114661T1 (de) | 1990-04-02 | 1994-12-15 | Pfizer | Benzylphosphonsäure-tyrosinkinaseinhibitoren. |
| US5302606A (en) | 1990-04-16 | 1994-04-12 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Styryl-substituted pyridyl compounds which inhibit EGF receptor tyrosine kinase |
| AU658646B2 (en) | 1991-05-10 | 1995-04-27 | Rhone-Poulenc Rorer International (Holdings) Inc. | Bis mono-and bicyclic aryl and heteroaryl compounds which inhibit EGF and/or PDGF receptor tyrosine kinase |
| JPH06503095A (ja) | 1991-05-29 | 1994-04-07 | ファイザー・インコーポレーテッド | 三環式ポリヒドロキシ系のチロシンキナーゼ阻害薬 |
| NZ243082A (en) | 1991-06-28 | 1995-02-24 | Ici Plc | 4-anilino-quinazoline derivatives; pharmaceutical compositions, preparatory processes, and use thereof |
| US5298422A (en) | 1991-11-06 | 1994-03-29 | Baylor College Of Medicine | Myogenic vector systems |
| GB9300059D0 (en) | 1992-01-20 | 1993-03-03 | Zeneca Ltd | Quinazoline derivatives |
| GB9205320D0 (en) | 1992-03-11 | 1992-04-22 | Ici Plc | Anti-tumour compounds |
| EP0654024A1 (en) | 1992-08-06 | 1995-05-24 | Warner-Lambert Company | 2-thioindoles (selenoindoles) and related disulfides (selenides) which inhibit protein tyrosine kinases and which have antitumor properties |
| US5330992A (en) | 1992-10-23 | 1994-07-19 | Sterling Winthrop Inc. | 1-cyclopropyl-4-pyridyl-quinolinones |
| US5366889A (en) * | 1992-11-30 | 1994-11-22 | The General Hospital Corporation | DNA encoding a protein-coupled receptor kinase |
| GB9226855D0 (en) | 1992-12-23 | 1993-02-17 | Erba Carlo Spa | Vinylene-azaindole derivatives and process for their preparation |
| GB9501567D0 (en) | 1995-01-26 | 1995-03-15 | Pharmacia Spa | Hydrosoluble 3-arylidene-2-oxindole derivatives as tyrosine kinase inhibitors |
| US5830699A (en) * | 1996-05-07 | 1998-11-03 | The General Hospital Corporation | SOK-1 and methods of use |
| AU4448597A (en) | 1996-10-04 | 1998-05-05 | Connaught Laboratories Limited | Treatment of cancer using a recombinant poxvirus containing nucleic acid sequence encoding a cytokine |
| AU8296698A (en) * | 1997-07-08 | 1999-02-08 | Cadus Pharmaceutical Corporation | (kds) protein kinase molecules and uses related thereto |
| AU8778698A (en) * | 1997-08-11 | 1999-03-01 | Ontogeny, Inc. | Serine/threonine kinase, and uses related thereto |
| GB9719920D0 (en) * | 1997-09-19 | 1997-11-19 | Zeneca Ltd | Human Ste20-Like Stress Activated Serine/Threonine Kinase |
| GB9726851D0 (en) * | 1997-12-19 | 1998-02-18 | Zeneca Ltd | Human signal transduction serine/threonine kinase |
| US6013500A (en) * | 1998-05-21 | 2000-01-11 | The Trustees Of Columbia University In The City Of New York | PAK4, a novel gene encoding a serine/threonine kinase |
-
1999
- 1999-04-13 DE DE69926742T patent/DE69926742T2/de not_active Expired - Fee Related
- 1999-04-13 WO PCT/US1999/008150 patent/WO1999053036A2/en not_active Ceased
- 1999-04-13 DK DK99918539T patent/DK1073723T3/da active
- 1999-04-13 US US09/291,417 patent/US6680170B2/en not_active Expired - Fee Related
- 1999-04-13 JP JP2000543584A patent/JP2002522009A/ja not_active Withdrawn
- 1999-04-13 AU AU36424/99A patent/AU3642499A/en not_active Abandoned
- 1999-04-13 ES ES99918539T patent/ES2248997T3/es not_active Expired - Lifetime
- 1999-04-13 AT AT99918539T patent/ATE302269T1/de not_active IP Right Cessation
- 1999-04-13 CA CA002369172A patent/CA2369172A1/en not_active Abandoned
- 1999-04-13 EP EP99918539A patent/EP1073723B1/en not_active Expired - Lifetime
-
2000
- 2000-10-16 US US09/688,188 patent/US6656716B1/en not_active Expired - Fee Related
-
2003
- 2003-12-02 US US10/725,329 patent/US20040224323A1/en not_active Abandoned
- 2003-12-02 US US10/725,121 patent/US20050142625A1/en not_active Abandoned
- 2003-12-02 US US10/725,330 patent/US20070072261A1/en not_active Abandoned
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006507835A (ja) | 2002-11-27 | 2006-03-09 | スージェン・インコーポレーテッド | リン酸特異的ｐａｋ抗体および診断キット |
| JP2012023006A (ja) * | 2010-07-15 | 2012-02-02 | Hyundai Motor Co Ltd | リチウム二次電池用陽極材料及びその製造方法並びにその材料を含むリチウム二次電池 |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE302269T1 (de) | 2005-09-15 |
| US6680170B2 (en) | 2004-01-20 |
| CA2369172A1 (en) | 1999-10-21 |
| US20050142625A1 (en) | 2005-06-30 |
| WO1999053036A2 (en) | 1999-10-21 |
| ES2248997T3 (es) | 2006-03-16 |
| DK1073723T3 (da) | 2006-01-02 |
| EP1073723A2 (en) | 2001-02-07 |
| WO1999053036A3 (en) | 2000-05-11 |
| AU3642499A (en) | 1999-11-01 |
| US20030050230A1 (en) | 2003-03-13 |
| DE69926742T2 (de) | 2006-06-14 |
| EP1073723B1 (en) | 2005-08-17 |
| US20040224323A1 (en) | 2004-11-11 |
| DE69926742D1 (de) | 2005-09-22 |
| US20070072261A1 (en) | 2007-03-29 |
| US6656716B1 (en) | 2003-12-02 |
| WO1999053036A9 (en) | 2000-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002522009A (ja) | Ｓｔｅ−２０関連蛋白質キナーゼ | |
| US6841579B1 (en) | Diagnosis and treatment of AUR1 and/or AUR2 related disorders | |
| JP3944241B2 (ja) | Ａｕｒ―１および／またはａｕｒ―２関連疾患の診断および処置 | |
| US20070202107A1 (en) | Novel kinases | |
| US20050125852A1 (en) | Novel kinases | |
| JP2003514583A (ja) | 新規ヒト蛋白質キナーゼおよび蛋白質キナーゼ様酵素 | |
| WO2000073469A2 (en) | Protein kinases | |
| JP2004504009A (ja) | 新規ヒト蛋白質キナーゼおよび蛋白質キナーゼ様酵素 | |
| JP2002518016A (ja) | Ｎｅｋ関連及びｂｕｂ１関連プロテインキナーゼ | |
| JP2003520602A (ja) | 新規ヒト蛋白質キナーゼおよび蛋白質キナーゼ様酵素 | |
| US6495353B1 (en) | Human orthologues of wart | |
| US6388063B1 (en) | Diagnosis and treatment of SAD related disorders | |
| US7119174B2 (en) | Diagnosis and treatment of AUR1 and/or AUR2 related disorders | |
| WO2001077338A2 (en) | Human protein kinases and protein kinase-like enzymes | |
| EP1595946A2 (en) | STE20-related protein kinases | |
| US20030211989A1 (en) | Novel human protein kinases and protein kinase-like enzymes | |
| US6342593B1 (en) | Diagnosis and treatment of ALP related disorders | |
| US20060204994A1 (en) | Novel human protein kinases and protein kinase-like enzymes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20050510 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20060327 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060327 |
|
| A761 | Written withdrawal of application |
Free format text: JAPANESE INTERMEDIATE CODE: A761 Effective date: 20060419 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20060419 |