JP2002513323A - 微孔質エレメントの製造方法、該方法により製造した微孔質エレメントおよびその使用 - Google Patents
微孔質エレメントの製造方法、該方法により製造した微孔質エレメントおよびその使用Info
- Publication number
- JP2002513323A JP2002513323A JP51127998A JP51127998A JP2002513323A JP 2002513323 A JP2002513323 A JP 2002513323A JP 51127998 A JP51127998 A JP 51127998A JP 51127998 A JP51127998 A JP 51127998A JP 2002513323 A JP2002513323 A JP 2002513323A
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- Japan
- Prior art keywords
- microporous
- solution
- solvent
- support
- polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Filtering Materials (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.以下の工程: a)物質を与えること;および b)その物質の少なくとも一部を多孔質形態に凝固させること を含む、微孔質エレメントを製造するための方法。 2.物質が液相で存在する請求項1に記載の方法。 3.液相が溶媒中のポリマーの溶液または懸濁液であり、多孔質形態での凝固 がポリマーに対する非溶媒の作用により引き起こされる、請求項2に記載の方法 。 4.ポリマーが、ポリビニルエステル、部分的に脱アシル化されたポリビニル エステル、セルロース誘導体、ポリアミド、ポリスチレン、ポリ(メチルメタク リレート)およびそれらの混合物からなる群から選択される、請求項3に記載の 方法。 5.ポリマーが、親水性および疎水性セグメントの両方をその分子内に含んで いる、請求項3または4に記載の方法。 6.ポリマーが、ポリ(ビニルアルコール−コ−エチレン)、ポリ(ビニルア ルコール−コ−酢酸ビニル)、エチレンアクリル酸コポリマー、エチレンアクリ ル酸エステルコポリマー、エチレンアクリルアミドコポリマー、アクリル酸酢酸 ビニルコポリマー、アクリルアミド酢酸ビニルコポリマー、アクリル酸ジアミン モノアミドと酢酸ビニルのコポリマー、ポリ(ビニルアルコール−コ−スチレン )、ポリ(スチレン−コ−マレイン酸)およびそのグリセリンエステル誘導体、 アクリルアミドアクリル酸エステルコポリマー、およびそれらの混合物からなる 群から選択される、請求項5に記載の方法。 7.溶媒が、ジメチルスルホキシド、ジメチルホルムアミド、ジメチルアセト アミド、ホルムアミド、ギ酸、酢酸、2,2,2−トリクロロエタノール、トルエ ン、テトラヒドロフランおよびそれらの混合物からなる群から選択される、請求 項3〜6のいずれかに記載の方法。 8.溶媒が少なくとも2つの揮発性の非溶媒からなる、請求項3〜7のいずれ かに記載の方法。 9.該溶媒が、室温でまたは真空下で除くことが可能である、請求項8に記載 の方法。 10.凝固が、溶媒および/または非溶媒を蒸発させることによって達成され る、請求項3〜9のいずれかに記載の方法。 11.非溶媒が、水、炭素原子数1〜4のアルコール、アンモニア、酢酸エチ ル、アセトン、エチレンジアミン、およびそれらの混合物からなる群から選択さ れる、請求項3〜10のいずれかに記載の方法。 12.液相がヒドロコロイドである、請求項2に記載の方法。 13.ヒドロコロイドが、低融点アガロース、デンプン、ポリビニルアルコ− ル、およびそれらの混合物からなる群から選択される、請求項12に記載の方法 。 14.ヒドロコロイド溶液の層を四ホウ酸ナトリウムによる架橋に付す、請求 項12または13に記載の方法。 15.凝固したヒドロコロイドを乾燥および/または架橋させる、請求項12 〜14のいずれかに記載の方法。 16.液相が1またはそれ以上のモノマーの溶液または懸濁液であり、凝固が 該モノマーを重合させることによって引き起こされる、請求項2に記載の方法。 17.モノマーが溶媒中に存在する、請求項16に記載の方法。 18.モノマーが1またはそれ以上の架橋用モノマーを含む、請求項16また は17に記載の方法。 19.該モノマーがジアミンおよびジエポキシドを含む、請求項18に記載の 方法。 20.モノマーがビニルエステル、アクリル酸およびその誘導体からなる群か ら選択され、重合がフリーラジカルの作用の下で引き起こされる、請求項16〜 19のいずれかに記載の方法。 21.物質に、流路形成流体または可逆性ヒドロゲル、または温度20℃以上 で水に不溶性である4000ダルトンまたはそれ以上のポリプロピレングリコ− ルなどの液体を浸透させる、請求項1〜20のいずれかに記載の方法。 22.流路形成流体が、シリコーンオイル、水または空気である、請求項21 に記載の方法。 23.物質が熱可塑性ポリマーであり、凝固が例えば加熱または溶媒の作用に よる焼結よって引き起こされる、請求項1に記載の方法。 24.ポリマーが、スチレンマレイン酸コポリマーまたはポリ酢酸ビニルであ る、請求項23に記載の方法。 25.物質が、さらに0.01μm〜500μmのサイズの多孔質または非孔 質の固体微粒子を含む、請求項1〜24のいずれかに記載の方法。 26.物質および微粒子が、低粘度の非溶媒中の懸濁液として存在する、請求 項25に記載の方法。 27.該物質および該微粒子を、貧溶媒中での非常に希薄な溶液からの蒸発に より、またはポリマー溶液なしで代わりに部分的溶融ポリマーのみを使用して1 30℃までの中程度の温度で焼結させることにより凝固させる、請求項26に記 載の方法。 28.該微粒子が、好ましくはイオン交換クロマトグラフィー、シリカゲルの 吸着クロマトグラフィー、逆相クロマトグラフィーおよび/または疎水性相互作 用クロマトグラフィーまたはアフィニティークロマトグラフィーに有用な最終の 微孔質エレメントの吸着特性を改変する、請求項25〜27のいずれかに記載の 方法。 29.該微粒子が、イオンを封鎖することができる、固体の支持体に選択的に 結合することができる、あらかじめ選択された抗原決定基に結合することができ る、またはリガンドであることができる、エフェクター分子を含む、請求項25 〜28のいずれかに記載の方法。 30.該分子が酵素または間接的に検出可能な部分を含む、請求項29に記載 の方法。 31.該分子が、アミノ酸、核酸またはその類縁体もしくは誘導体の配列を含 む、請求項29または30に記載の方法。 32.該イオンを封鎖することができる分子が、カルモジュリン、メタロチオ ネイン、その断片、またはグルタミン酸、アスパラギン酸、リジンおよびアルギ ニンのうちの少なくとも1つに富むアミノ酸配列である、請求項29〜31のい ずれかに記載の方法。 33.該あらかじめ選択された抗原決定基に結合することができるエフェクタ ー分子が、抗体または断片またはその誘導体である、請求項29〜32のいずれ かに記載の方法。 34.該固体の支持体に選択的に結合することができる分子が、GST、Hi s−tag、Lex−Aである、請求項29〜33のいずれかに記載の方法。 35.該リガンドがNi−NTAである、請求項29〜34のいずれかに記載 の方法。 36.該物質が自己支持的であって支持体に加えられる、請求項1〜35のい ずれかに記載の方法。 37.支持体が、ポリプロピレン(PP)、ポリエチレン(PE)、プロピレ ン/エチレンコポリマー、ポリ酢酸ビニル、ポリアミド、ポリスチレン、ポリエ チレンテレフタレート(PET)、ポリエーテルエーテルケトン(PEEK)、 ポリカーボネート、ポリエチレン酢酸ビニル、ポリ(ビニルアルコール−コ−エ チレン)、ポリエステル、ポリアミド、ガラス、セラミック、水晶、窒化ケイ素 、またはそれらの混合物、またはそれらの、ガラス、二酸化ケイ素、炭素もしく はセラミックの繊維もしくはフレームとの複合材料から形成される、請求項36 に記載の方法。 38.支持体が管の形態を有し、微孔質エレメントがその管の一端またはその 端の近くで生成する、請求項36または37に記載の方法。 39.その管の少なくとも一断面が、小さい横断面と大きい横断面を有する円 錐状の形態であり、微孔質エレメントが小さい方の横断面の端またはその近くで 生成する、請求項38に記載の方法。 40.溶液を孔に加える工程が、溶液を毛管作用によって管の中を上昇させる ことにより行なわれる、請求項38または39に記載の方法。 41.管の内壁が親水性コーティングで被覆されている、請求項38〜40の いずれかに記載の方法。 42.微孔質エレメントに隣接する管の端が親水性コーティングされずに保た れている、請求項41に記載の方法。 43.コーティングが、有機溶媒中の1またはそれ以上のポリビニルエステル の溶液を管の内壁に塗布し、有機溶媒を蒸発させ、生じたポリビニルエステルの 層をその表面で部分的に加水分解することにより形成される、請求項41に記載 の方法。 ポリ(スチレン−コ−マレイン酸)のポリオール誘導体もまた有用である。 44.支持体の口径が0.02mm〜4mmである、請求項36〜43のいず れかに記載の方法。 45.物質を保持体に加える、請求項1〜44のいずれかに記載の方法。 46.保持体が微孔質である、請求項45に記載の方法。 47.微孔質保持体が、お互いに連結した固体粒子を含む、請求項45または 46に記載の方法。 48.微孔質保持体が、ポリエチレン(PE)、ポリプロピレン(PP)、プ ロピレン/エチレンコポリマー、ポリ酢酸ビニル、ポリアミド、ポリスチレン、 ポリエチレンテレフタレート(PET)、ポリエーテルエーテルケトン(PEE K)、ポリカーボネート、ポリ(ビニルアルコール−コ−エチレン)、ポリエス テル、ポリアミド、ガラス、セラミック、水晶、窒化ケイ素、またはそれらの混 合物、ステンレススチール、またはそれらの、ガラス、二酸化ケイ素、炭素もし くはセラミックの繊維もしくはフレームとの複合材料から形成される、請求項4 5〜47のいずれかに記載の方法。 49.微孔質保持体が、ディスク、格子、大孔の膜、支持繊維を有する膜、織 布または不織布の性質を有する膜、ネット、プレート、棒および/または先端が 切り取られた円錐の形態である、請求項45〜48のいずれかに記載の方法。 50.微孔質エレメントを支持体の中で生じさせる、請求項36〜49のいず れかに記載の方法。 51.支持体が中空で水分不浸透性である、請求項36〜50のいずれかに記 載の方法。 52.微孔質保持体の上に少なくとも1つの微孔質膜を配置する工程をさらに 含む、請求項45〜51のいずれかに記載の方法。 53.微孔質膜が、(再生された)セルロース、ポリアミド、ポリエステル、 ポリプロピレン(PP)またはポリテトラフルオロエチレン(PTFE)から形 成される、請求項52に記載の方法。 54.該保持体および/または該支持体が凝固後に取り除かれる、請求項36 〜53のいずれかに記載の方法。 55.請求項1〜54のいずれかに記載の方法にしたがって得られる微孔質エ レメント。 56.微孔質エレメントが、ディスク、格子、支持繊維を有する膜、織布また は不織布の性質を有する膜、ネット、プレート、棒または先端が切り取られた円 錐の形態である、請求項55に記載の微孔質エレメント。 57.請求項1〜54のいずれかに記載の方法にしたがって得られる請求項5 5または56に記載の微孔質エレメントを含むフィルターエレメント。 58.ポリマーが、微孔質保持体の外側表面の少なくとも1部分に付着してい る、請求項57に記載のフィルターエレメント。 59.微孔質保持体が、その孔にポリマーを、流体がフィルターエレメントを 流れることを可能にする流路を形成している残存したフリーの空間をその孔が有 するように含んでいる、請求項57または58に記載のフィルターエレメント。 60.微粒子の平均直径が、孔の平均直径の50%未満である、請求項59に 記載のフィルターエレメント。 61.請求項57〜60のいずれかに記載の複数の本発明のフィルターエレメ ントを含んでなる多重流路フィルターエレメント。 62.請求項55または56に記載の微孔質エレメントまたは請求項57〜6 1のいずれかに記載のフィルターエレメントを含むキット。 63.請求項55または56に記載の微孔質エレメントまたは請求項57〜6 1のいずれかに記載のフィルターエレメントを含む診断用組成物。 64.請求項55または56に記載の微孔質エレメントまたは請求項57〜6 1のいずれかに記載のフィルターエレメントを含む医薬組成物。 65.マイクロ濾過、固体−液体分離、溶液の清浄化、原核および真核細胞の 回収、細胞断片および破片の除去および精製、断片、ウイルスおよびプラスミド の固定化、タンパク質、ポリペプチド、核酸、オリゴヌクレオチドの吸着/固定 化のための、または粒状物質の保持体としての、請求項55または56に記載の 微孔質エレメントまたは請求項57〜61のいずれかに記載のフィルターエレメ ントの使用。 66.アフィニティークロマトグラフィー、免疫クロマトグラフィー等の目的 のため、結合試験のための多成分が結合した複合体の分離のため、試料のスクリ ーニングのため、または微粒子の位置が変わることを防止するための、請求項5 5または56に記載の微孔質エレメントまたは請求項57〜61のいずれかに記 載のフィルターエレメントの使用。 67.核酸、オリゴヌクレオチド、ポリペプチド、またはポリサッカライド、 タンパク質、有機化合物、またはそれらの誘導体もしくは類縁体を含む化合物の 調製および/または検出のための、請求項65または66に記載の使用。 68.該化合物が天然または非天然の供給源に由来する、請求項67に記載の 使用。
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-
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- 1996-12-17 EP EP96120279A patent/EP0826412A3/en not_active Withdrawn
-
1997
- 1997-08-26 ES ES97942879T patent/ES2395388T3/es not_active Expired - Lifetime
- 1997-08-26 AU AU44557/97A patent/AU4455797A/en not_active Abandoned
- 1997-08-26 JP JP51127998A patent/JP4387462B2/ja not_active Expired - Lifetime
- 1997-08-26 EP EP97942879A patent/EP0958035B1/en not_active Expired - Lifetime
- 1997-08-26 WO PCT/EP1997/004653 patent/WO1998008594A2/en active Application Filing
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JP2005533146A (ja) * | 2002-07-13 | 2005-11-04 | クランフィールド ユニヴァーシティー | 分子インプリントポリマー材料 |
JP2005176716A (ja) * | 2003-12-19 | 2005-07-07 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
JP2005205333A (ja) * | 2004-01-23 | 2005-08-04 | Hokuetsu Paper Mills Ltd | 分子吸着機能を有するシート |
JP4541715B2 (ja) * | 2004-01-23 | 2010-09-08 | 北越紀州製紙株式会社 | 分子吸着機能を有するシート |
JP2006296220A (ja) * | 2005-04-15 | 2006-11-02 | Gl Sciences Inc | Dnaなどの分離精製方法及び分離精製機構 |
JP2015224225A (ja) * | 2014-05-28 | 2015-12-14 | 京セラ株式会社 | 金属化合物吸着材およびそれを用いた金属化合物の回収方法 |
JP2018512133A (ja) * | 2015-03-19 | 2018-05-17 | スリーエム イノベイティブ プロパティズ カンパニー | 流体試料中の微生物株又は標的細胞検体を検出するためのデバイス、方法、キット、及びシステム |
JP2018039949A (ja) * | 2016-09-09 | 2018-03-15 | 日立化成株式会社 | 刺激応答性ポリマー |
WO2018047634A1 (ja) * | 2016-09-09 | 2018-03-15 | 日立化成株式会社 | 刺激応答性ポリマー |
JP2020505226A (ja) * | 2017-01-24 | 2020-02-20 | ザルトリウス ステディム ビオテック ゲーエムベーハー | 微小球が結合したクロマトグラフィー媒体、及びその製造方法 |
US11364480B2 (en) | 2017-01-24 | 2022-06-21 | Sartorius Stedim Biotech Gmbh | Chromatography medium with bound microglobules and method for the preparation thereof |
Also Published As
Publication number | Publication date |
---|---|
EP0958035A2 (en) | 1999-11-24 |
EP0826412A2 (en) | 1998-03-04 |
AU4455797A (en) | 1998-03-19 |
WO1998008594A2 (en) | 1998-03-05 |
JP4387462B2 (ja) | 2009-12-16 |
EP0958035B1 (en) | 2012-11-07 |
ES2395388T3 (es) | 2013-02-12 |
EP0826412A3 (en) | 1999-06-02 |
WO1998008594A3 (en) | 1998-05-07 |
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