JP2000506721A - 核転移用レシピエント細胞質としての不活性卵母細胞 - Google Patents
核転移用レシピエント細胞質としての不活性卵母細胞Info
- Publication number
- JP2000506721A JP2000506721A JP9509995A JP50999597A JP2000506721A JP 2000506721 A JP2000506721 A JP 2000506721A JP 9509995 A JP9509995 A JP 9509995A JP 50999597 A JP50999597 A JP 50999597A JP 2000506721 A JP2000506721 A JP 2000506721A
- Authority
- JP
- Japan
- Prior art keywords
- embryo
- animal
- nucleus
- oocytes
- embryos
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 86
- 210000000805 cytoplasm Anatomy 0.000 title claims abstract description 13
- 238000012546 transfer Methods 0.000 title claims description 24
- 238000000034 method Methods 0.000 claims abstract description 69
- 210000004027 cell Anatomy 0.000 claims abstract description 59
- 241001465754 Metazoa Species 0.000 claims abstract description 58
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 51
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 46
- 230000031864 metaphase Effects 0.000 claims abstract description 16
- KYRVNWMVYQXFEU-UHFFFAOYSA-N Nocodazole Chemical compound C1=C2NC(NC(=O)OC)=NC2=CC=C1C(=O)C1=CC=CS1 KYRVNWMVYQXFEU-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229950006344 nocodazole Drugs 0.000 claims abstract description 10
- 230000004913 activation Effects 0.000 claims description 45
- 241000283690 Bos taurus Species 0.000 claims description 25
- 241001494479 Pecora Species 0.000 claims description 12
- 102000029749 Microtubule Human genes 0.000 claims description 7
- 108091022875 Microtubule Proteins 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 7
- 210000004688 microtubule Anatomy 0.000 claims description 7
- 230000007910 cell fusion Effects 0.000 claims description 5
- 230000005937 nuclear translocation Effects 0.000 claims description 4
- 229930012538 Paclitaxel Natural products 0.000 claims description 3
- 229960001592 paclitaxel Drugs 0.000 claims description 3
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 claims description 3
- 241000283707 Capra Species 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000005764 inhibitory process Effects 0.000 claims 2
- 241000282836 Camelus dromedarius Species 0.000 claims 1
- 241000282898 Sus scrofa Species 0.000 claims 1
- 230000012106 negative regulation of microtubule depolymerization Effects 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 230000006641 stabilisation Effects 0.000 claims 1
- 238000011105 stabilization Methods 0.000 claims 1
- 230000022131 cell cycle Effects 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 11
- 231100000782 microtubule inhibitor Toxicity 0.000 abstract description 9
- 230000010190 G1 phase Effects 0.000 abstract description 8
- 230000009261 transgenic effect Effects 0.000 abstract description 8
- 230000035519 G0 Phase Effects 0.000 abstract description 7
- 206010027476 Metastases Diseases 0.000 abstract description 5
- 230000002068 genetic effect Effects 0.000 abstract description 5
- 230000009401 metastasis Effects 0.000 abstract description 5
- 229940122255 Microtubule inhibitor Drugs 0.000 abstract description 4
- 210000004940 nucleus Anatomy 0.000 description 49
- 230000035800 maturation Effects 0.000 description 20
- 238000011161 development Methods 0.000 description 19
- 230000018109 developmental process Effects 0.000 description 19
- 210000002459 blastocyst Anatomy 0.000 description 17
- 230000004927 fusion Effects 0.000 description 17
- 238000000338 in vitro Methods 0.000 description 11
- 238000001727 in vivo Methods 0.000 description 11
- 230000008901 benefit Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 241000894007 species Species 0.000 description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 7
- 241000124008 Mammalia Species 0.000 description 7
- 229910052791 calcium Inorganic materials 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 230000001086 cytosolic effect Effects 0.000 description 7
- 230000007159 enucleation Effects 0.000 description 7
- 210000004602 germ cell Anatomy 0.000 description 7
- 108010077544 Chromatin Proteins 0.000 description 6
- 210000003483 chromatin Anatomy 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 210000004508 polar body Anatomy 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 5
- 238000012239 gene modification Methods 0.000 description 5
- 230000005017 genetic modification Effects 0.000 description 5
- 235000013617 genetically modified food Nutrition 0.000 description 5
- 230000011278 mitosis Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 4
- 241000282887 Suidae Species 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 230000021121 meiosis Effects 0.000 description 4
- 230000008672 reprogramming Effects 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 3
- 229930195725 Mannitol Natural products 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000711408 Murine respirovirus Species 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000007894 caplet Substances 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000003325 follicular Effects 0.000 description 3
- 244000144980 herd Species 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000000594 mannitol Substances 0.000 description 3
- 235000010355 mannitol Nutrition 0.000 description 3
- 238000000520 microinjection Methods 0.000 description 3
- 210000003101 oviduct Anatomy 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000004043 responsiveness Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 241001550206 Colla Species 0.000 description 2
- 230000006820 DNA synthesis Effects 0.000 description 2
- 235000019687 Lamb Nutrition 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 102000004243 Tubulin Human genes 0.000 description 2
- 108090000704 Tubulin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 210000001109 blastomere Anatomy 0.000 description 2
- 239000012888 bovine serum Substances 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000025090 microtubule depolymerization Effects 0.000 description 2
- 230000000394 mitotic effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 210000000633 nuclear envelope Anatomy 0.000 description 2
- 238000010449 nuclear transplantation Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- 230000001776 parthenogenetic effect Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 210000004340 zona pellucida Anatomy 0.000 description 2
- NNJPGOLRFBJNIW-HNNXBMFYSA-N (-)-demecolcine Chemical compound C1=C(OC)C(=O)C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-HNNXBMFYSA-N 0.000 description 1
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 235000002198 Annona diversifolia Nutrition 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000282832 Camelidae Species 0.000 description 1
- 206010068051 Chimerism Diseases 0.000 description 1
- NNJPGOLRFBJNIW-UHFFFAOYSA-N Demecolcine Natural products C1=C(OC)C(=O)C=C2C(NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-UHFFFAOYSA-N 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- 230000010337 G2 phase Effects 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000282838 Lama Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229940119336 Microtubule stabilizer Drugs 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 208000034790 Twin pregnancy Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000010428 chromatin condensation Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001152 differential interference contrast microscopy Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 210000002503 granulosa cell Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000029115 microtubule polymerization Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000027291 mitotic cell cycle Effects 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000010539 reproductive behavior Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000012409 spindle disassembly Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8773—Ovine embryos
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Animal Behavior & Ethology (AREA)
- Environmental Sciences (AREA)
- Immunology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gynecology & Obstetrics (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9517779.6 | 1995-08-31 | ||
| GBGB9517779.6A GB9517779D0 (en) | 1995-08-31 | 1995-08-31 | Biological manipulation |
| PCT/GB1996/002098 WO1997007668A1 (en) | 1995-08-31 | 1996-08-30 | Unactivated oocytes as cytoplast recipients for nuclear transfer |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005345998A Division JP2006340710A (ja) | 1995-08-31 | 2005-11-30 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
| JP2008111492A Division JP2008194053A (ja) | 1995-08-31 | 2008-04-22 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000506721A true JP2000506721A (ja) | 2000-06-06 |
| JP2000506721A5 JP2000506721A5 (cg-RX-API-DMAC7.html) | 2004-09-24 |
Family
ID=10779997
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9509995A Pending JP2000506721A (ja) | 1995-08-31 | 1996-08-30 | 核転移用レシピエント細胞質としての不活性卵母細胞 |
| JP2005345998A Pending JP2006340710A (ja) | 1995-08-31 | 2005-11-30 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
| JP2008111492A Pending JP2008194053A (ja) | 1995-08-31 | 2008-04-22 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005345998A Pending JP2006340710A (ja) | 1995-08-31 | 2005-11-30 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
| JP2008111492A Pending JP2008194053A (ja) | 1995-08-31 | 2008-04-22 | 核移植用レシピエント細胞質としての不活性卵母細胞 |
Country Status (22)
| Country | Link |
|---|---|
| US (12) | US6252133B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP0847237B1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP2000506721A (cg-RX-API-DMAC7.html) |
| KR (4) | KR20070041793A (cg-RX-API-DMAC7.html) |
| CN (3) | CN1813642B (cg-RX-API-DMAC7.html) |
| AT (1) | ATE400176T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU728809B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR9610013A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2229657A1 (cg-RX-API-DMAC7.html) |
| CZ (1) | CZ60498A3 (cg-RX-API-DMAC7.html) |
| DE (1) | DE69637591D1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK0847237T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2309989T3 (cg-RX-API-DMAC7.html) |
| GB (1) | GB9517779D0 (cg-RX-API-DMAC7.html) |
| HU (1) | HUP9802485A3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL123417A0 (cg-RX-API-DMAC7.html) |
| NO (1) | NO980846L (cg-RX-API-DMAC7.html) |
| NZ (2) | NZ335407A (cg-RX-API-DMAC7.html) |
| PL (1) | PL325336A1 (cg-RX-API-DMAC7.html) |
| SG (1) | SG75919A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO1997007668A1 (cg-RX-API-DMAC7.html) |
| ZA (1) | ZA967383B (cg-RX-API-DMAC7.html) |
Families Citing this family (193)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5480772A (en) | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517779D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US7307198B2 (en) | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
| US6077697A (en) | 1996-04-10 | 2000-06-20 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| CA2267220A1 (en) | 1996-10-11 | 1998-04-23 | The Texas A & M University System | Methods for the generation of primordial germ cells and transgenic animal species |
| US6235969B1 (en) * | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US20090092588A1 (en) * | 1997-01-10 | 2009-04-09 | University Of Massachusetts As Represented By Its Amherst Campus | Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| EP1000161B1 (en) | 1997-03-26 | 2006-02-22 | Imperial College Innovations Limited | Anticoagulant fusion protein anchored to cell membrane |
| IL133786A0 (en) | 1997-07-03 | 2001-04-30 | Univ Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
| US7923015B2 (en) | 1997-08-14 | 2011-04-12 | Institut Pasteur | Methods for direct visualization of active synapses |
| US7923216B2 (en) | 1997-08-14 | 2011-04-12 | Institut Pasteur | In vivo modulation of neuronal transport |
| JPH11127852A (ja) * | 1997-10-28 | 1999-05-18 | Snow Brand Milk Prod Co Ltd | 上皮細胞のｇ０期同調方法 |
| WO1999036510A1 (de) * | 1998-01-16 | 1999-07-22 | Agrobiogen Gmbh | Effizienter kerntransfer mit fetalen fibroblasten |
| US7351876B2 (en) | 1998-01-16 | 2008-04-01 | Agrobiogen Gmbh Biotechnologie | Efficient nuclear transfer with primordial gametes |
| CA2318117A1 (en) * | 1998-01-16 | 1999-07-22 | Agrobiogen Gmbh | Efficient nuclear transfer using primordial germ cells |
| US6331659B1 (en) * | 1998-01-21 | 2001-12-18 | University Of Hawaii | Cumulus cells as nuclear donors |
| WO1999046982A1 (en) * | 1998-03-16 | 1999-09-23 | Relag Pty Ltd | Porcine nuclear transfer |
| AUPP294898A0 (en) * | 1998-04-15 | 1998-05-07 | Monash University | Method of nuclear transfer |
| GB9808325D0 (en) * | 1998-04-20 | 1998-06-17 | Ltr Ciz Di Associazione Italia | Source of nuclei for nuclear transfer |
| US6143564A (en) * | 1998-07-07 | 2000-11-07 | University Of Hawaii | Use of the polar body chromosomes for the production of embryos and normal offspring |
| GB9820988D0 (en) * | 1998-09-25 | 1998-11-18 | Biotech & Biolog Scien Res | Screening method |
| AU6218899A (en) * | 1998-10-12 | 2000-05-01 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
| US6781030B1 (en) * | 1998-11-02 | 2004-08-24 | Trustee Of Tufts College, Ballou Hall | Methods for cloning mammals using telophase oocytes |
| EP1818397A1 (en) * | 1998-11-02 | 2007-08-15 | Trustees Of Tufts College | Methods for cloning animals |
| DE69940700D1 (de) | 1998-11-19 | 2009-05-20 | Pharming Intellectual Pty Bv | Stabilisierung der milch transgener tieren |
| US6700037B2 (en) | 1998-11-24 | 2004-03-02 | Infigen, Inc. | Method of cloning porcine animals |
| US6258998B1 (en) | 1998-11-24 | 2001-07-10 | Infigen, Inc. | Method of cloning porcine animals |
| JP2002534118A (ja) * | 1999-01-13 | 2002-10-15 | ピーピーエル セラピューティクス （スコットランド） リミテッド | 二重核移植法及びその結果物 |
| US7531715B1 (en) * | 1999-01-13 | 2009-05-12 | Ppl Therapeutics (Scotland) | Double nuclear transfer method and results thereof |
| EP2314700A1 (en) | 1999-01-28 | 2011-04-27 | Medical College of Georgia Research Institute, Inc | Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA |
| WO2000050443A2 (en) | 1999-02-26 | 2000-08-31 | Millennium Pharmaceutcals, Inc. | Secreted proteins and uses thereof |
| US20020012660A1 (en) * | 1999-03-04 | 2002-01-31 | Alan Colman | Method of preparing a somatic cells for nuclear transfer |
| WO2000051424A2 (en) * | 1999-03-04 | 2000-09-08 | Ppl Therapeutics (Scotland) Ltd. | Genetic modification of somatic cells and uses thereof |
| AU5553600A (en) | 1999-06-23 | 2001-01-31 | Ppl Therapeutics (Scotland) Ltd | Fusion proteins incorporating lysozyme |
| KR100414043B1 (ko) * | 1999-06-30 | 2004-01-13 | 황우석 | 체세포 복제동물 및 그 생산방법 |
| KR100368435B1 (ko) * | 1999-06-30 | 2003-01-24 | 황우석 | 체세포 복제동물의 생산을 위한 세포융합방법 |
| US7291714B1 (en) | 1999-06-30 | 2007-11-06 | Millennium Pharmaceuticals, Inc. | Glycoprotein VI and uses thereof |
| CA2334954A1 (en) * | 1999-06-30 | 2001-01-04 | Woo-Suk Hwang | A method for producing cloned tigers by employing inter-species nuclear transplantation technique |
| RU2216591C2 (ru) * | 1999-06-30 | 2003-11-20 | Воо-Сук ХВАНГ | Способ получения клонированных эмбрионов человека путем применения способа межвидовой трансплантации ядер |
| WO2001000795A1 (en) * | 1999-06-30 | 2001-01-04 | Hwang, Woo-Suk | A method for producing cloned cows |
| PT1210428E (pt) | 1999-08-23 | 2015-07-21 | Genetics Inst Llc | Pd-1, um recetor para b7-4 e suas utilizações |
| EP2275559A3 (en) | 1999-09-28 | 2011-03-23 | Shire Human Genetic Therapies, Inc. | Optimized messenger RNA |
| WO2002064799A2 (en) | 1999-09-28 | 2002-08-22 | Transkaryotic Therapies, Inc. | Optimized messenger rna |
| WO2001035735A1 (en) * | 1999-11-19 | 2001-05-25 | Hematech, Llc | Production of ungulates, preferably bovines that produce human immunoglobulins |
| US7414170B2 (en) | 1999-11-19 | 2008-08-19 | Kirin Beer Kabushiki Kaisha | Transgenic bovines capable of human antibody production |
| GB9927444D0 (en) * | 1999-11-19 | 2000-01-19 | Cancer Res Campaign Tech | Inhibiting gene expression |
| US7074983B2 (en) * | 1999-11-19 | 2006-07-11 | Kirin Beer Kabushiki Kaisha | Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin |
| US7820878B2 (en) * | 1999-11-19 | 2010-10-26 | Kyowa Hakko Kirin Co., Ltd. | Production of ungulates, preferably bovines that produce human immunoglobulins |
| KR100417566B1 (ko) * | 1999-11-19 | 2004-02-05 | 한국생명공학연구원 | 체세포 핵치환 복제수정란의 대량생산방법 |
| EP1254210A4 (en) | 2000-01-04 | 2003-07-02 | Univ Connecticut | METHOD FOR CLONING ANIMALS WITH TARGETED GENETIC MODIFICATIONS, BY TRANSFERRING LONG-TERM CULTURAL SOMATIC OR MALE SOMATIC CELLS, INCLUDING ARTIFICIALLY CAUSED GENETIC MODIFICATIONS, IN ENCEPTED RECEIVED CELLS |
| US6635802B1 (en) * | 2000-01-10 | 2003-10-21 | The Texas A&M University System | Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors |
| DK1248640T3 (da) | 2000-01-20 | 2007-02-12 | Diabcell Pty Ltd | Xenotransplantation af svineöer og deres fremstilling |
| WO2001059076A2 (en) * | 2000-02-09 | 2001-08-16 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Premeiotic and postmeiotic origin of teratomas: isolated teratoma stem cells for therapeutic uses |
| US6906238B2 (en) | 2000-03-15 | 2005-06-14 | University Of Georgia Research Foundation, Inc. | Effective nuclear reprogramming in mammals using CDK2 inhibitors |
| GB2360522A (en) | 2000-03-24 | 2001-09-26 | Geron Corp | A strategy for maintaining pregnancy |
| WO2001088096A2 (en) * | 2000-05-15 | 2001-11-22 | Geron Corporation | Ovine tissue for xenotransplantation |
| ATE439453T1 (de) | 2000-06-05 | 2009-08-15 | Genetics Inst Llc | Zusammensetzungen, testsätze und methoden zur identifizierung und modulation von typ i diabetes |
| NZ522844A (en) | 2000-06-28 | 2005-02-25 | Brigham & Womens Hospital | PD-L2 molecules: novel PD-1 ligands and methods to identify compounds to modulate T cell activation |
| EP1311530A4 (en) | 2000-08-03 | 2004-10-06 | Wim-Van Schooten | PRODUCTION OF HUMANIZED ANTIBODIES IN TRANSGENIC ANIMALS |
| AU2001284948A1 (en) | 2000-08-15 | 2002-02-25 | Board Of Trustees Of The University Of Illinois | Animals expressing exogenous igf-i in their milk |
| US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
| FR2814642B1 (fr) | 2000-10-03 | 2005-07-01 | Ass Pour Le Dev De La Rech En | Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee |
| CA2425076A1 (en) * | 2000-10-06 | 2002-04-11 | Advanced Cell Technology, Inc. | Cloning endangered and extinct species |
| IL155951A0 (en) | 2000-11-28 | 2003-12-23 | Wyeth Corp | Expression analysis of kiaa nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer |
| JP2004526425A (ja) | 2000-11-28 | 2004-09-02 | ワイス | 前立腺癌の診断および治療に有用なｆｋｂｐ核酸およびポリペプチドの発現分析 |
| CA2430627A1 (en) * | 2000-11-30 | 2002-12-27 | Stemron, Inc. | Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same |
| US7491534B2 (en) | 2000-12-22 | 2009-02-17 | Kirin Holdings Kabushiki Kaisha | Methods for altering cell fate to generate T-cells specific for an antigen of interest |
| US20020142397A1 (en) * | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
| EP2138583A1 (en) | 2000-12-22 | 2009-12-30 | Institut National De La Recherche Agronomique | Position-independent and tissue specific expression of a transgene in milk of transgenic animals |
| NZ525607A (en) | 2000-12-22 | 2005-05-27 | Aurox Llc | Methods for cloning mammals using reprogrammed donor chromatin or donor cells |
| EP1860199B1 (en) | 2001-01-12 | 2014-03-05 | Exelixis, Inc. | Modulating insulin receptor signaling |
| US20050177883A1 (en) | 2001-01-30 | 2005-08-11 | Rebholtz Paul C. | Systems of transferring embryos and managing recipients |
| EP1229134A3 (en) | 2001-01-31 | 2004-01-28 | Nucleonics, Inc | Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell |
| JP2002262716A (ja) * | 2001-03-07 | 2002-09-17 | National Agricultural Research Organization | 株化細胞を用いて家畜個体を作出する方法 |
| US8981061B2 (en) | 2001-03-20 | 2015-03-17 | Novo Nordisk A/S | Receptor TREM (triggering receptor expressed on myeloid cells) and uses thereof |
| US7126039B2 (en) * | 2001-03-21 | 2006-10-24 | Geron Corporation | Animal tissue with carbohydrate antigens compatible for human transplantation |
| US7265262B2 (en) * | 2001-03-21 | 2007-09-04 | Roslin Institute (Edinburgh) | Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer |
| US7105328B2 (en) | 2001-04-02 | 2006-09-12 | Dana-Farber Cancer Institute | Methods for screening for compounds that modulate pd-1 signaling |
| BR0208944A (pt) | 2001-04-16 | 2006-10-10 | Wyeth Corp | quadros de leitura aberta de streptococus pneumoniae codificando os antìgenos de polipeptìdeos e seus usos |
| EP1405068A4 (en) * | 2001-06-14 | 2007-09-26 | Infigen Inc | PROCESS FOR CLONING MAMMALS USING REMODELING FACTORS |
| EP1406917A4 (en) * | 2001-06-15 | 2007-11-14 | New Century Pharmaceuticals | HUMAN ANALYTICAL MODELS FOR THE EVALUATION OF MEDICINAL PRODUCTS AND FOR TOXICOLOGICAL AND IMMUNOGENIC EXAMINATIONS |
| EP1412371B1 (en) | 2001-07-12 | 2016-02-24 | University of Massachusetts | IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING |
| EP1900815B1 (en) | 2001-07-12 | 2016-09-07 | University of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
| US20030211603A1 (en) * | 2001-08-14 | 2003-11-13 | Earp David J. | Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells |
| US20030170678A1 (en) * | 2001-10-25 | 2003-09-11 | Neurogenetics, Inc. | Genetic markers for Alzheimer's disease and methods using the same |
| AU2002364945A1 (en) * | 2001-10-25 | 2003-07-09 | Neurogenetics, Inc. | Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases |
| US20030224380A1 (en) * | 2001-10-25 | 2003-12-04 | The General Hospital Corporation | Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases |
| WO2003046129A2 (en) * | 2001-11-09 | 2003-06-05 | Trustees Of The University Of Pennsylvania | Compositions for the derivation of germ cells from stem cells and methods of use thereof |
| US7091006B2 (en) | 2001-11-09 | 2006-08-15 | Dana-Farber Cancer Institute, Inc. | PGC-1β, a novel PGC-1 homologue and uses therefor |
| AU2003235652A1 (en) * | 2002-01-15 | 2003-07-30 | Advanced Cell Technology, Inc. | Cloning b and t lymphocytes |
| US20050090004A1 (en) * | 2003-01-16 | 2005-04-28 | Sayre Chauncey B. | Stem cell maturation for all tissue lines |
| US20050170506A1 (en) * | 2002-01-16 | 2005-08-04 | Primegen Biotech Llc | Therapeutic reprogramming, hybrid stem cells and maturation |
| US20030134422A1 (en) * | 2002-01-16 | 2003-07-17 | Sayre Chauncey Bigelow | Stem cell maturation for all tissue lines |
| CN1650003A (zh) * | 2002-04-01 | 2005-08-03 | Gtc生物治疗学公司 | 选择用于哺乳动物种中核转移的细胞系的方法 |
| US20030217378A1 (en) * | 2002-04-05 | 2003-11-20 | The University Of Georgia Research Foundation, Inc | Cloning using rapidly matured oocytes |
| US7612250B2 (en) * | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
| CN102220423A (zh) | 2002-08-20 | 2011-10-19 | 千年药品公司 | 用于鉴定,评估,预防和治疗子宫颈癌的组合物,试剂盒及方法 |
| CA2501752A1 (en) | 2002-10-10 | 2004-04-22 | Wyeth | Compositions, organisms and methodologies employing a novel human kinase |
| AU2003286442A1 (en) * | 2002-10-16 | 2004-05-04 | Advanced Cell Technology, Inc. | Methods using gene trapped stem cells for marking pathways of stem cell differentiation and making and isolating differentiated cells |
| WO2004038026A2 (en) | 2002-10-24 | 2004-05-06 | Wyeth | Calcineurin-like human phosphoesterase |
| MXPA05004870A (es) | 2002-11-08 | 2005-11-04 | Hematech Llc | Ungulados transgenicos que tienen actividad reducida de la proteina prion y sus usos. |
| JP2006514554A (ja) | 2002-11-21 | 2006-05-11 | ワイス | 腎細胞癌および他の固形腫瘍の診断法 |
| WO2004050831A2 (en) | 2002-11-27 | 2004-06-17 | Wyeth | Compositions, organisms and methodologies employing a novel human kinase |
| EP2474631B1 (en) | 2002-12-20 | 2014-02-12 | Celera Corporation | Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof |
| SI1581812T1 (sl) | 2003-01-06 | 2008-10-31 | Wyeth Corp | Sestavki in postopki za diagnosticiranje in zdravljenje raka kolona |
| CA2527958C (en) | 2003-06-02 | 2014-04-08 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of rnai |
| EP1633767B1 (en) | 2003-06-02 | 2018-11-21 | University of Massachusetts | Methods and compositions for controlling efficacy of rna silencing |
| JP2007502118A (ja) | 2003-08-14 | 2007-02-08 | エクセリクシス， インク． | βカテニン経路のモディファイヤーとしてのＳＵＬＦｓ及び使用方法 |
| MXPA06003402A (es) | 2003-10-07 | 2006-06-27 | Millennium Pharm Inc | Moleculas de acidos nucleicos y proteinas para la identificacion, evaluacion, prevencion y tratamiento de cancer de ovario. |
| US7951532B2 (en) | 2003-11-12 | 2011-05-31 | Children's Hospital Medical Center | Method of screening a midkine modulating agent |
| CA2832293C (en) | 2003-11-26 | 2015-08-04 | Celera Corporation | Single nucleotide polymorphisms associated with cardiovascular disorders and statin response, methods of detection and uses thereof |
| US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
| NZ550106A (en) | 2004-04-22 | 2009-06-26 | Kyowa Hakko Kirin Co Ltd | Transgenic animals and uses thereof |
| EP2423326B1 (en) | 2004-05-07 | 2014-01-08 | Celera Corporation | Genetic polymorphism associated with liver fibrosis methods of detection and uses thereof |
| JP2008517931A (ja) | 2004-10-22 | 2008-05-29 | ダナ−ファーバー キャンサー インスティチュート，インコーポレイテッド | 脂質関連疾患および障害を処置するための、ＰＧＣ−１βを調節するための方法および組成物 |
| GB0426146D0 (en) | 2004-11-29 | 2004-12-29 | Bioxell Spa | Therapeutic peptides and method |
| CN1274829C (zh) | 2004-12-10 | 2006-09-13 | 四川大学华西医院 | 抗eb病毒所致肿瘤多肽及其应用与制备方法 |
| EP1856296A2 (en) | 2005-03-11 | 2007-11-21 | Applera Corporation | Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof |
| US20070011603A1 (en) * | 2005-07-06 | 2007-01-11 | Mikko Makela | Method, system, device and software product for showing tooltips for page segments and generating content for the page segments |
| NZ565711A (en) | 2005-08-09 | 2011-10-28 | Revivicor Inc | Transgenic ungulates expressing a porcine CTLA4 peptide fused to an immunoglobulin |
| EP3031470A3 (en) | 2005-09-09 | 2016-08-10 | The Johns Hopkins University | Manipulation of regulatory t cell and dc function by targeting neuritin gene using antibodies, agonists and antagonists |
| CA2623106C (en) | 2005-09-19 | 2013-12-24 | Histogenics Corporation | Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof |
| US7799530B2 (en) | 2005-09-23 | 2010-09-21 | Celera Corporation | Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof |
| US7695911B2 (en) | 2005-10-26 | 2010-04-13 | Celera Corporation | Genetic polymorphisms associated with Alzheimer's Disease, methods of detection and uses thereof |
| WO2008027986A2 (en) | 2006-09-01 | 2008-03-06 | Therapeutic Human Polyclonals, Inc. | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals |
| EP2639319B1 (en) | 2006-09-11 | 2015-07-08 | Celera Corporation | Genetic polymorphisms associated with Psoriasis, methods of detection and uses thereof |
| CA2915679C (en) | 2006-10-20 | 2017-12-12 | Celera Corporation | Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof |
| KR101398220B1 (ko) * | 2006-10-30 | 2014-05-22 | 건국대학교 산학협력단 | 형질전환 배아 생성방법 |
| US20100205678A1 (en) * | 2007-01-05 | 2010-08-12 | Overstrom Eric W | Oocyte spindle-associated factors improve somatic cell cloning |
| MX352541B (es) | 2007-04-07 | 2017-11-29 | Whitehead Inst Biomedical Res | Reprogramación de células somáticas. |
| WO2008134522A1 (en) * | 2007-04-26 | 2008-11-06 | President And Fellows Of Harvard College | Deriving embryonic stem cells |
| WO2008144580A2 (en) * | 2007-05-17 | 2008-11-27 | Oregon Health & Science University | Primate stem cells produced by somatic cell nuclear transfer |
| US20090111764A1 (en) * | 2007-10-25 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Mitochondrial selection |
| US8663980B2 (en) | 2007-10-26 | 2014-03-04 | Janssen Biotech, Inc. | Vectors, host cells, and methods of production and uses |
| US8039212B2 (en) | 2007-11-05 | 2011-10-18 | Celera Corporation | Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof |
| US20090221620A1 (en) | 2008-02-20 | 2009-09-03 | Celera Corporation | Gentic polymorphisms associated with stroke, methods of detection and uses thereof |
| KR100915397B1 (ko) * | 2008-02-22 | 2009-09-03 | 삼성전자주식회사 | 커버부재, 현상 카트리지 및 화상형성장치의 현상유닛 |
| JP5670755B2 (ja) | 2008-03-12 | 2015-02-18 | ザ ロックフェラー ユニバーシティ | 翻訳プロファイリング及び分子表現型解析のための方法及び組成物 |
| US8288094B2 (en) | 2008-04-09 | 2012-10-16 | Institut Pasteur | APOBEC3 mediated DNA editing |
| CA2969377C (en) | 2008-06-13 | 2019-12-03 | Whitehead Institute For Biomedical Research | Programming and reprogramming of cells |
| WO2010006215A1 (en) | 2008-07-09 | 2010-01-14 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
| US9181315B2 (en) | 2009-01-08 | 2015-11-10 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for induced brown fat differentiation |
| WO2010124200A2 (en) | 2009-04-23 | 2010-10-28 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for cancer |
| US9434921B2 (en) | 2009-04-24 | 2016-09-06 | Oregon Health & Science University | Methods for mitochondrial DNA replacement in oocytes |
| EP2267153A1 (en) | 2009-05-26 | 2010-12-29 | Université Claude Bernard Lyon 1 | Identification of netrin-1 receptor unc5c gene mutation in solid cancers |
| WO2011002988A1 (en) | 2009-07-01 | 2011-01-06 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for severe combined immunodeficiency (scid) |
| EP2456876A2 (en) | 2009-07-24 | 2012-05-30 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for cytokine-cytokine signaling pathways |
| US20110145936A1 (en) | 2009-07-30 | 2011-06-16 | Ostertag Eric M | Genetically Modified Rat Models for Pharmacokinetics |
| EP2461672A2 (en) | 2009-08-05 | 2012-06-13 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for drug metabolism |
| US20120151609A1 (en) | 2009-08-20 | 2012-06-14 | Ostertag Eric M | Genetically Modified Rat Models for Pain |
| US9420770B2 (en) | 2009-12-01 | 2016-08-23 | Indiana University Research & Technology Corporation | Methods of modulating thrombocytopenia and modified transgenic pigs |
| WO2011091134A2 (en) | 2010-01-22 | 2011-07-28 | Dana-Farber Cancer Institute, Inc. | Compositions,kits, and methods for identification, assessment, prevention, and therapy of metabolic disorders |
| TWI518325B (zh) | 2010-02-04 | 2016-01-21 | 自治醫科大學 | 對ａｌｋ抑制劑具有先抗性或後抗性癌症的識別、判斷和治療 |
| US10745467B2 (en) | 2010-03-26 | 2020-08-18 | The Trustees Of Dartmouth College | VISTA-Ig for treatment of autoimmune, allergic and inflammatory disorders |
| US20150231215A1 (en) | 2012-06-22 | 2015-08-20 | Randolph J. Noelle | VISTA Antagonist and Methods of Use |
| MX342017B (es) | 2010-03-26 | 2016-09-09 | Dartmouth College | Proteina mediadora de celula t regulatoria vista, agentes de enlace de vista y uso de los mismos. |
| US8383793B2 (en) | 2010-04-15 | 2013-02-26 | St. Jude Children's Research Hospital | Methods and compositions for the diagnosis and treatment of cancer resistant to anaplastic lymphoma kinase (ALK) kinase inhibitors |
| US20110269735A1 (en) | 2010-04-19 | 2011-11-03 | Celera Corporation | Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof |
| CN103179860A (zh) | 2010-10-22 | 2013-06-26 | 阿格里-尼奥公司 | 过乙酸和至少一种sar诱导剂用于防治生长植物之中和之上的病原体的协同活性 |
| US20120108651A1 (en) | 2010-11-02 | 2012-05-03 | Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) | Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof |
| WO2012064090A2 (ko) | 2010-11-08 | 2012-05-18 | 광주과학기술원 | 골격근-유래된 다능성 세포의 화학적 제조방법 및 이의 용도 |
| WO2012112586A1 (en) | 2011-02-14 | 2012-08-23 | Revivicor, Inc. | Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof |
| WO2012129198A1 (en) | 2011-03-23 | 2012-09-27 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for obesity and diabetes |
| MX383372B (es) | 2011-05-16 | 2025-03-13 | Univ Missouri | Animales resistentes al virus del sindrome reproductivo y respiratorio porcino. |
| US10093705B2 (en) | 2011-09-13 | 2018-10-09 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for brown fat induction and activity using FNDC5 |
| WO2013120553A1 (en) | 2012-02-15 | 2013-08-22 | Novo Nordisk A/S | Antibodies that bind and block triggering receptor expressed on myeloid cells-1 (trem-1) |
| US9550830B2 (en) | 2012-02-15 | 2017-01-24 | Novo Nordisk A/S | Antibodies that bind and block triggering receptor expressed on myeloid cells-1 (TREM-1) |
| WO2013120554A1 (en) | 2012-02-15 | 2013-08-22 | Novo Nordisk A/S | Antibodies that bind peptidoglycan recognition protein 1 |
| JP6285923B2 (ja) | 2012-06-22 | 2018-02-28 | トラスティーズ・オブ・ダートマス・カレッジ | 新規ＶＩＳＴＡ−Ｉｇコンストラクト及び自己免疫、アレルギー性及び炎症性障害の処置のためのＶＩＳＴＡ−Ｉｇの使用 |
| US9890215B2 (en) | 2012-06-22 | 2018-02-13 | King's College London | Vista modulators for diagnosis and treatment of cancer |
| EP3552628A1 (en) | 2012-09-07 | 2019-10-16 | The Trustees Of Dartmouth College | Vista modulators for diagnosis and treatment of cancer |
| US10273301B2 (en) | 2013-12-24 | 2019-04-30 | Janssen Pharmaceutica Nv | Anti-vista antibodies and fragments |
| US11014987B2 (en) | 2013-12-24 | 2021-05-25 | Janssen Pharmaceutics Nv | Anti-vista antibodies and fragments, uses thereof, and methods of identifying same |
| CA2935804A1 (en) | 2014-01-14 | 2015-07-23 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for identification, assessment, prevention, and treatment of melanoma using pd-l1 isoforms |
| US11123426B2 (en) | 2014-06-11 | 2021-09-21 | The Trustees Of Dartmouth College | Use of vista agonists and antagonists to suppress or enhance humoral immunity |
| CN113214397A (zh) | 2014-07-17 | 2021-08-06 | 诺和诺德股份有限公司 | 定点诱变trem-1抗体以降低黏度 |
| US10077420B2 (en) | 2014-12-02 | 2018-09-18 | Histogenics Corporation | Cell and tissue culture container |
| MX2017007136A (es) | 2014-12-05 | 2017-12-04 | Immunext Inc | Identificacion de vsig8 como el receptor vista putativo y su uso para producir moduladores vista/vsig8. |
| LT3230320T (lt) | 2014-12-09 | 2021-01-11 | Regeneron Pharmaceuticals, Inc. | Gyvūnai, išskyrus žmogų, turintys humanizuotą diferenciacijos klasterio 274 geną |
| US10136642B2 (en) | 2015-02-19 | 2018-11-27 | Agri-Neo, Inc. | Composition of peracetic acid and at least one organic fungicide for the control and/or the treatment of diseases associated with the presence of pathogens, and method, use and kit involving said composition |
| CN114805576A (zh) | 2015-06-24 | 2022-07-29 | 詹森药业有限公司 | 抗vista抗体和片段 |
| PL3331355T3 (pl) | 2015-08-06 | 2024-08-05 | The Curators Of The University Of Missouri | Zwierzęta z rodzaju świń odporne na wirusa zespołu rozrodczo- oddechowego świń (prrsv) i komórki mające zmodyfikowane geny cd163 |
| US12194080B2 (en) | 2015-10-29 | 2025-01-14 | Dana-Farber Cancer Institute, Inc. | Methods for identification, assessment, prevention, and treatment of metabolic disorders using PM20D1 and N-lipidated amino acids |
| MX2018009800A (es) | 2016-02-12 | 2018-11-09 | Janssen Pharmaceutica Nv | Anticuerpos y fragmentos anti-vista, usos de los mismos y procedimientos de identificacion de los mismos. |
| PE20190115A1 (es) | 2016-04-15 | 2019-01-16 | Immunext Inc | Anticuerpos vista antihumanos y su uso |
| EP3774902A1 (en) | 2018-04-02 | 2021-02-17 | Bristol-Myers Squibb Company | Anti-trem-1 antibodies and uses thereof |
| US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
| IL302983A (en) | 2020-11-20 | 2023-07-01 | Revivicor Inc | Multitransgenic pigs with growth hormone receptor silencing for xenotransplantation |
| CN118434281A (zh) | 2021-09-20 | 2024-08-02 | 雷维维科公司 | 用于异种移植的包含十个基因修饰的多转基因猪 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ224241A (en) * | 1987-04-14 | 1991-03-26 | Bresatec Ltd | Method of producing new animal breeds using plasmid expression vectors; plasmid expression vectors |
| US5057420A (en) * | 1987-06-05 | 1991-10-15 | Granada Biosciences, Inc. | Bovine nuclear transplantation |
| CA2059199A1 (en) | 1992-01-10 | 1993-07-11 | Steen Willadsen | Method for producing mature reconstituted mammalian eggs by nuclear transplantation |
| GB2265909B (en) | 1992-04-01 | 1996-03-20 | Inst Of Animal Physiology And | Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion |
| EP0661969B1 (en) * | 1992-09-22 | 2003-03-12 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Taxol for treating lymphomas and breast cancer and for reducing multi-drug resistance to taxol |
| CA2092258A1 (en) | 1992-12-30 | 1994-07-01 | Steven L. Stice | Synchronized donor cells for bovine nuclear transfer |
| US5480772A (en) | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
| US5496720A (en) | 1993-02-10 | 1996-03-05 | Susko-Parrish; Joan L. | Parthenogenic oocyte activation |
| US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| US5333948A (en) | 1993-06-22 | 1994-08-02 | Tekonsha Engineering Company | Multiple-gain electronic brake actuator with trigger point inertial sensor |
| ES2210258T5 (es) * | 1993-07-29 | 2009-01-16 | The Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | Procedimiento de tratamiento de la ateroesclerosis o reestenosis usando un agente estabilizante de microtubulos. |
| ATE213771T1 (de) | 1993-12-23 | 2002-03-15 | Infigen Inc | Embryonale stammzellen von huftieren als kerndonatoren und kerntransfertechniken zur herstellung chimärer und transgener tiere |
| GB9417831D0 (en) | 1994-09-05 | 1994-10-26 | Biotech & Biolog Scien Res | Biological manipulation |
| GB9517780D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517779D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US7307198B2 (en) | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
| US6215041B1 (en) * | 1997-01-10 | 2001-04-10 | University Of Mmassachusetts | Cloning using donor nuclei from a non-quiesecent somatic cells |
| US6235969B1 (en) * | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US7291764B1 (en) | 1997-01-10 | 2007-11-06 | University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst | Cloning pigs using non-quiescent differentiated donor cells or nuclei |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US7361804B1 (en) * | 1997-02-19 | 2008-04-22 | Roslin Institute (Edinburgh) | Unactivated oocytes in nuclear transfer to produce ungulates |
| US6011197A (en) * | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| WO1999036510A1 (de) | 1998-01-16 | 1999-07-22 | Agrobiogen Gmbh | Effizienter kerntransfer mit fetalen fibroblasten |
| JP3573964B2 (ja) * | 1998-06-17 | 2004-10-06 | 三洋電機株式会社 | アルカリ電池用水素吸蔵合金電極及びアルカリ蓄電池用水素吸蔵合金電極の製造方法 |
| AU6218899A (en) * | 1998-10-12 | 2000-05-01 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
| US6700037B2 (en) | 1998-11-24 | 2004-03-02 | Infigen, Inc. | Method of cloning porcine animals |
| US20050156424A1 (en) * | 2000-02-29 | 2005-07-21 | Thyssenkrupp Presta Ag | Steering column and manufacturing method thereof |
| US8302202B2 (en) * | 2005-08-03 | 2012-10-30 | International Business Machines Corporation | Transportable computing environment apparatus system and method |
-
1995
- 1995-08-31 GB GBGB9517779.6A patent/GB9517779D0/en active Pending
-
1996
- 1996-08-30 AU AU68309/96A patent/AU728809B2/en not_active Expired
- 1996-08-30 DE DE69637591T patent/DE69637591D1/de not_active Expired - Lifetime
- 1996-08-30 NZ NZ335407A patent/NZ335407A/en not_active IP Right Cessation
- 1996-08-30 BR BR9610013-3A patent/BR9610013A/pt not_active Application Discontinuation
- 1996-08-30 NZ NZ316148A patent/NZ316148A/xx not_active IP Right Cessation
- 1996-08-30 KR KR1020077007531A patent/KR20070041793A/ko not_active Withdrawn
- 1996-08-30 IL IL12341796A patent/IL123417A0/xx unknown
- 1996-08-30 HU HU9802485A patent/HUP9802485A3/hu unknown
- 1996-08-30 WO PCT/GB1996/002098 patent/WO1997007668A1/en not_active Ceased
- 1996-08-30 CZ CZ98604A patent/CZ60498A3/cs unknown
- 1996-08-30 CN CN2006100037660A patent/CN1813642B/zh not_active Expired - Lifetime
- 1996-08-30 CA CA002229657A patent/CA2229657A1/en not_active Abandoned
- 1996-08-30 KR KR1019980701382A patent/KR100533477B1/ko not_active Expired - Lifetime
- 1996-08-30 SG SG1999000941A patent/SG75919A1/en unknown
- 1996-08-30 CN CNA2005101270278A patent/CN1772891A/zh active Pending
- 1996-08-30 CN CNB961978929A patent/CN1250715C/zh not_active Expired - Lifetime
- 1996-08-30 ZA ZA9607383A patent/ZA967383B/xx unknown
- 1996-08-30 EP EP96928586A patent/EP0847237B1/en not_active Expired - Lifetime
- 1996-08-30 DK DK96928586T patent/DK0847237T3/da active
- 1996-08-30 KR KR1020057014691A patent/KR100743006B1/ko not_active Expired - Lifetime
- 1996-08-30 KR KR1020067006296A patent/KR20060057638A/ko not_active Ceased
- 1996-08-30 JP JP9509995A patent/JP2000506721A/ja active Pending
- 1996-08-30 AT AT96928586T patent/ATE400176T1/de not_active IP Right Cessation
- 1996-08-30 ES ES96928586T patent/ES2309989T3/es not_active Expired - Lifetime
- 1996-08-30 PL PL96325336A patent/PL325336A1/xx unknown
-
1997
- 1997-02-19 US US08/803,165 patent/US6252133B1/en not_active Expired - Lifetime
-
1998
- 1998-02-27 NO NO980846A patent/NO980846L/no unknown
-
2000
- 2000-08-29 US US09/650,285 patent/US6525243B1/en not_active Expired - Lifetime
-
2001
- 2001-10-11 US US09/973,701 patent/US20030037352A1/en not_active Abandoned
-
2002
- 2002-07-09 US US10/190,617 patent/US7326824B2/en not_active Expired - Fee Related
- 2002-09-05 US US10/234,854 patent/US7321075B2/en not_active Expired - Fee Related
-
2004
- 2004-08-03 US US10/909,845 patent/US7329796B2/en not_active Expired - Fee Related
- 2004-08-10 US US10/914,161 patent/US7321076B2/en not_active Expired - Fee Related
-
2005
- 2005-03-02 US US11/068,903 patent/US7432415B2/en not_active Expired - Fee Related
- 2005-11-03 US US11/265,114 patent/US7524677B2/en not_active Expired - Fee Related
- 2005-11-30 JP JP2005345998A patent/JP2006340710A/ja active Pending
-
2006
- 2006-10-06 US US11/544,038 patent/US20070028312A1/en not_active Abandoned
- 2006-10-06 US US11/543,881 patent/US7326825B2/en not_active Expired - Fee Related
-
2008
- 2008-04-22 JP JP2008111492A patent/JP2008194053A/ja active Pending
-
2009
- 2009-12-24 US US12/647,316 patent/US20100146654A1/en not_active Abandoned
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2000506721A (ja) | 核転移用レシピエント細胞質としての不活性卵母細胞 | |
| US7304204B2 (en) | Ungulates produced by nuclear transfer of G1 cells | |
| US7361804B1 (en) | Unactivated oocytes in nuclear transfer to produce ungulates | |
| AU2005202808B2 (en) | Unactivated oocytes as cytoplast recipients for nuclear transfer | |
| AU2005202805B2 (en) | Unactivated oocytes as cytoplast recipients for nuclear transfer | |
| GB2340493A (en) | Unactivated oocytes as cytoplast recipients for nuclear transfer | |
| NZ504101A (en) | A method of preparing a non-human mammal comprising reconstituting a non-human mammalian embryo | |
| MXPA98001645A (en) | Oocytes inactivated as receptors of cytopllasto for nuclear transfer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20051125 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070116 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20070330 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20070521 |
|
| A524 | Written submission of copy of amendment under article 19 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A524 Effective date: 20070713 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20071225 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080422 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080618 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20080618 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20080807 |
|
| A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20080828 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20101202 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20101207 |