IN2014KN00946A - Method and apparatus for chromatographic purification - Google Patents

Method and apparatus for chromatographic purification

Info

Publication number
IN2014KN00946A
IN2014KN00946A IN946/KOLNP/2014A IN946KON2014A IN2014KN00946A IN 2014KN00946 A IN2014KN00946 A IN 2014KN00946A IN 946KON2014 A IN946KON2014 A IN 946KON2014A IN 2014KN00946 A IN2014KN00946 A IN 2014KN00946A
Authority
IN
India
Prior art keywords
chromatographic purification
chromatographic
sequentially
separation columns
polishing
Prior art date
Application number
IN946/KOLNP/2014A
Other languages
English (en)
Inventor
Romas Skudas
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44936136&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IN2014KN00946(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of IN2014KN00946A publication Critical patent/IN2014KN00946A/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G01N30/467—Flow patterns using more than one column with separation columns in parallel all columns being identical
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
IN946/KOLNP/2014A 2011-10-04 2014-05-02 Method and apparatus for chromatographic purification IN2014KN00946A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11008021.5A EP2578286A1 (de) 2011-10-04 2011-10-04 Verfahren und Vorrichtung zur chromatographischen Aufreinigung
US201261666338P 2012-06-29 2012-06-29
PCT/EP2012/003866 WO2013050104A1 (en) 2011-10-04 2012-09-15 Method and apparatus for chromatographic purification

Publications (1)

Publication Number Publication Date
IN2014KN00946A true IN2014KN00946A (en) 2015-10-09

Family

ID=44936136

Family Applications (1)

Application Number Title Priority Date Filing Date
IN946/KOLNP/2014A IN2014KN00946A (en) 2011-10-04 2014-05-02 Method and apparatus for chromatographic purification

Country Status (10)

Country Link
US (2) US20140251911A1 (de)
EP (2) EP2578286A1 (de)
JP (1) JP6105598B2 (de)
KR (1) KR101974676B1 (de)
CN (1) CN103842045B (de)
CA (1) CA2850872C (de)
ES (1) ES2601889T3 (de)
IN (1) IN2014KN00946A (de)
SG (1) SG2014015226A (de)
WO (1) WO2013050104A1 (de)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2682168A1 (de) 2012-07-02 2014-01-08 Millipore Corporation Reinigung biologischer Moleküle
JP2014213313A (ja) * 2013-04-30 2014-11-17 独立行政法人日本原子力研究開発機構 濾過用基布
ES2665645T3 (es) * 2013-05-06 2018-04-26 Sanofi Proceso multietapa continuo para purificar anticuerpos
JP2016519145A (ja) 2013-05-15 2016-06-30 メディミューン リミテッド 組換え産生ポリペプチドの精製
GB201401010D0 (en) * 2014-01-21 2014-03-05 Nat Nuclear Lab Ltd Improved separation apparatus and method
EP3105580B1 (de) * 2014-02-14 2023-10-04 Cytiva Sweden AB Mehrstufiges automatisches reinigungssystem
JP6331485B2 (ja) * 2014-03-04 2018-05-30 株式会社島津製作所 液体クロマトグラフ及び液体クロマトグラフ分析方法
EP4147761A1 (de) 2014-04-30 2023-03-15 Novo Nordisk A/S Verfahren zur reinigung von proteinen mittels caprylsäure
EP3145951A1 (de) * 2014-06-24 2017-03-29 InSight Biopharmaceuticals Ltd. Verfahren zur reinigung von antikörpern
KR20170026521A (ko) * 2014-06-25 2017-03-08 제이에이치엘 바이오테크 인코포레이티드 단백질의 정제를 위한 방법 및 시약
EP3182117B1 (de) * 2014-08-12 2019-04-24 AiSTI SCIENCE CO., Ltd. Voranalysebehandlungsvorrichtung verwendbar für aminosäure-, organischen säure- und zucker sowie voranalysebehandlungsverfahren
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
EP3230729B1 (de) * 2014-12-12 2024-03-06 Cytiva Sweden AB System zur vorbereitung von mobilen phase in chromatographie
CN104729907B (zh) * 2015-01-26 2017-10-17 上虞市创烨生物有限公司 一种快速纯化糖化白蛋白粗溶液的方法
WO2016156153A1 (en) * 2015-03-31 2016-10-06 Ge Healthcare Bio-Sciences Ab A liquid chromatograhy system, a device, and a method
WO2017039673A1 (en) * 2015-09-03 2017-03-09 Hygeia Industries, Inc. Improved method for preparative and commercial scale reverse phase liquid chromatography
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10513537B2 (en) 2016-05-11 2019-12-24 Ge Healthcare Bioprocess R&D Ab Separation matrix
WO2017194593A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of cleaning and/or sanitizing a separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
CN109311949B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 储存分离基质的方法
CN121314243A (zh) 2016-08-26 2026-01-13 思拓凡生物工艺研发有限公司 层析系统
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201622343D0 (en) * 2016-12-29 2017-02-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
GB201702856D0 (en) * 2017-02-22 2017-04-05 Ge Healthcare Bio Sciences Ab Method in continuous chromatography
JP6790963B2 (ja) * 2017-03-30 2020-11-25 株式会社島津製作所 液体クロマトグラフ
WO2018203548A1 (ja) 2017-05-01 2018-11-08 日産化学株式会社 水溶性が改善されたアニオン性高分子化合物の調製方法
CN111050809B (zh) * 2017-07-10 2021-12-24 武田药品工业株式会社 孵育液体和灭活病毒的方法
WO2019213072A1 (en) * 2018-04-30 2019-11-07 Agilent Technologies, Inc. Multidimensional sample separation with pressure adjustment
US20210371852A1 (en) * 2018-05-18 2021-12-02 Children's National Medical Center System of cell expansion and methods of using the same
TWI870412B (zh) * 2019-06-05 2025-01-21 美商建南德克公司 過載層析管柱之再生方法
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
GB201911685D0 (en) * 2019-08-15 2019-10-02 Fujifilm Diosynth Biotechnologies Uk Ltd Process for purifying monoclonal antibodies
GB201911687D0 (en) 2019-08-15 2019-10-02 Fujifilm Diosynth Biotechnologies Uk Ltd Apparatus for purifying a liquid comprising a target substance
CN111610247B (zh) * 2020-05-27 2021-03-16 中国科学院地质与地球物理研究所 一种从地质样品中快速分离出高纯度w的方法
CN111643928B (zh) * 2020-06-12 2021-05-25 中国地质大学（武汉） 一种自动固相萃取装置
US12050448B2 (en) 2021-01-29 2024-07-30 Tata Consultancy Services Limited Method and system to control a continuous bioprocessing
EP4299756A4 (de) * 2021-03-09 2025-03-12 JCR Pharmaceuticals Co., Ltd. Verfahren zur herstellung eines antikörperlysosomalen enzymfusionsproteins
CA3208770A1 (en) * 2021-03-10 2022-09-15 Amgen Inc. Parallel chromatography systems and methods
KR20240046607A (ko) 2021-09-28 2024-04-09 메르크 파텐트 게엠베하 연속 크로마토그래피 작업 동안의 크로마토그래피 수지의 모니터링 방법
US20250179466A1 (en) * 2022-03-03 2025-06-05 Chromacon Ag Chromatographic purification method and uses thereof
CN114832439A (zh) * 2022-06-07 2022-08-02 杭州奕安济世生物药业有限公司 一种自动控制连续层析上样载量的方法和层析方法
EP4397323A1 (de) * 2023-01-09 2024-07-10 Sartorius Stedim Biotech GmbH Verfahren zur inaktivierung von viren in einem fluid
CN121712573A (zh) * 2023-07-04 2026-03-20 瑞士杰特贝林生物制品有限公司 纯化方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51104893A (ja) * 1975-03-12 1976-09-17 Hitachi Ltd Saijunkankuromatogurafuiihohooyobisochi
DE2914807A1 (de) * 1979-04-11 1980-10-23 Dionex Corp Verfahren und vorrichtung fuer die chromatographische separierung und quantitative analyse einer vielzahl von ionenarten in einer probenloesung
IE64511B1 (en) 1988-03-11 1995-08-09 Takeda Chemical Industries Ltd Automated synthesizing apparatus
US5205845A (en) * 1990-09-28 1993-04-27 The Regents Of The University Of Michigan Mechanical gas chromatography injection valves and column multiplexing techniques
WO1997001755A2 (en) * 1995-06-26 1997-01-16 Perseptive Biosystems, Inc. High speed, automated, continuous flow, multi-dimensional molecular selection and analysis
US5630943A (en) * 1995-11-30 1997-05-20 Merck Patent Gesellschaft Mit Beschrankter Haftung Discontinuous countercurrent chromatographic process and apparatus
US6214221B1 (en) 1999-02-22 2001-04-10 Henry B. Kopf Method and apparatus for purification of biological substances
US6375851B1 (en) 2000-05-05 2002-04-23 United States Filter Corporation Continuous liquid purification process
US20040002081A1 (en) * 2001-12-18 2004-01-01 Boehringer Ingelheim International Gmbh And Bia Separations D.O.O. Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
CN102050865B (zh) 2002-09-13 2014-03-12 拜奥根Idec公司 通过模拟移动床层析纯化多肽的方法
EP1614693A4 (de) * 2003-03-31 2006-07-19 Kirin Brewery Reinigung eines humanen monoklonalen antikörpers und humanen polyklonalen antikörpers
WO2005001589A1 (en) 2003-06-06 2005-01-06 Waters Investments Limited Apparatus and methods for performing parallel processes
WO2006043895A1 (en) * 2004-10-21 2006-04-27 Ge Healthcare Bio-Sciences Ab A method of antibody purification
JP2006125856A (ja) 2004-10-26 2006-05-18 Sumitomo Chemical Co Ltd 液体クロマトグラフィー装置
DE102005001162A1 (de) * 2005-01-10 2006-07-20 Haemosys Gmbh Adsorptionssystem zur Entfernung von Viren und viralen Bestandteilen aus Flüssigkeiten, insbesondere aus Blut und Blutplasma
JP2007085830A (ja) 2005-09-21 2007-04-05 Daicel Chem Ind Ltd 濃縮装置及びそれを有するクロマトグラフィー装置
EP1775001A1 (de) 2005-10-13 2007-04-18 Xendo Holding B.V. Vorrichtung für chromatographische Trennungen
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
MX2009007632A (es) 2007-01-22 2009-07-24 Genentech Inc Precipitacion de polielectrolito y purificacion de proteinas.
US20090149638A1 (en) 2007-10-03 2009-06-11 Ley Arthur C Systems and methods for purifying proteins
US8074494B2 (en) * 2007-12-10 2011-12-13 Proxeon Biosystems A/S Plugged flow 2D chromatography
CN101619090A (zh) * 2008-07-02 2010-01-06 中国科学院大连化学物理研究所 用于生物大分子分离纯化的柱切换循环体积排阻色谱系统
US20110232373A1 (en) 2008-12-04 2011-09-29 Vrije Universiteit Brussel Chromatographic separation device with variable length and a method for its use
US20110020327A1 (en) 2008-12-16 2011-01-27 Millipore Corporation Purification of proteins
EP2360183B1 (de) * 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antikörperreinigungsverfahren mittels "mixed-mode" chromatography unter verwendung einer arginine-haltigen beladungslösung
WO2010151214A1 (en) 2009-06-26 2010-12-29 Ge Healthcare Bio-Sciences Ab A method in a chromatography system
CN102574911B (zh) 2009-08-07 2017-06-06 Emd密理博公司 从样品的一或多种杂质中纯化靶蛋白的方法
US9527010B2 (en) 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
KR101152036B1 (ko) 2010-04-27 2012-06-08 (주)셀트리온 컬럼 순환 및 시료 연속주입법을 이용한 단백질 ａ 크로마토그래피 정제 방법
JP5400226B2 (ja) 2010-09-10 2014-01-29 株式会社日立製作所 計算機システムに対する処理のタスクでありユーザ操作に基づくタスクを管理するシステム、及び、その種のタスクに関する情報を表示する方法
EP2649016B1 (de) 2010-12-06 2020-06-10 Pall Corporation Verfahren zur kontinuierlichen verarbeitung biologischer produkte

Also Published As

Publication number Publication date
JP2014534055A (ja) 2014-12-18
CA2850872A1 (en) 2013-04-11
KR20140072162A (ko) 2014-06-12
US20200101399A1 (en) 2020-04-02
KR101974676B1 (ko) 2019-05-02
CN103842045A (zh) 2014-06-04
CA2850872C (en) 2019-10-01
WO2013050104A1 (en) 2013-04-11
ES2601889T3 (es) 2017-02-16
CN103842045B (zh) 2016-08-17
EP2763771B1 (de) 2016-08-03
JP6105598B2 (ja) 2017-03-29
EP2763771A1 (de) 2014-08-13
SG2014015226A (en) 2014-07-30
US20140251911A1 (en) 2014-09-11
US11590434B2 (en) 2023-02-28
EP2578286A1 (de) 2013-04-10

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