IN190327B - - Google Patents

Info

Publication number
IN190327B
IN190327B IN586MA2000A IN190327B IN 190327 B IN190327 B IN 190327B IN 586MA2000 A IN586MA2000 A IN 586MA2000A IN 190327 B IN190327 B IN 190327B
Authority
IN
India
Prior art keywords
periplasm
medium
prokaryotic
secretes
secreted
Prior art date
Application number
Other languages
English (en)
Inventor
Dorothee Ambrosius
Rainer Rudolph
Joerg Schaeffner
Elisabeth Schwarz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of IN190327B publication Critical patent/IN190327B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
IN586MA2000 1999-07-29 2000-07-26 IN190327B (ko)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP99114811A EP1077263A1 (de) 1999-07-29 1999-07-29 Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen

Publications (1)

Publication Number Publication Date
IN190327B true IN190327B (ko) 2003-07-19

Family

ID=8238680

Family Applications (1)

Application Number Title Priority Date Filing Date
IN586MA2000 IN190327B (ko) 1999-07-29 2000-07-26

Country Status (13)

Country Link
US (1) US6455279B1 (ko)
EP (2) EP1077263A1 (ko)
JP (1) JP2001061487A (ko)
KR (1) KR100378325B1 (ko)
CN (1) CN1231595C (ko)
AT (1) ATE299942T1 (ko)
CA (1) CA2313248C (ko)
DE (1) DE60021318T2 (ko)
DK (1) DK1077262T3 (ko)
ES (1) ES2247990T3 (ko)
IN (1) IN190327B (ko)
JO (1) JO2309B1 (ko)
MX (1) MXPA00007339A (ko)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001261242B2 (en) * 2000-05-03 2006-12-14 Expressive Constructs, Inc. A method for improving recombinant protein stability and solubility
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0027779D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods for large scale production of recombinant dna-derived tpa or k2s molecules
US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
US7094884B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them
US7094757B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
AU2002317841A1 (en) * 2001-06-22 2003-01-08 Roche Diagnostics Gmbh Use of fkbp chaperones as expression tool
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
KR100441755B1 (ko) * 2001-07-24 2004-08-02 재단법인서울대학교산학협력재단 분자 샤페론을 핵 내부로 이동시키는 방법
MXPA04001566A (es) * 2001-08-27 2004-05-17 Genentech Inc Un sistema para expresion y ensamblado de anticuerpos.
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
AU2003287345A1 (en) * 2002-10-31 2004-06-07 Genentech, Inc. Methods and compositions for increasing antibody production
WO2004065417A2 (en) * 2003-01-23 2004-08-05 Genentech, Inc. Methods for producing humanized antibodies and improving yield of antibodies or antigen binding fragments in cell culture
WO2005004894A2 (en) * 2003-05-12 2005-01-20 Expressive Constructs, Inc. Methods for increasing cell and tissue viability
US20050152894A1 (en) * 2003-09-05 2005-07-14 Genentech, Inc. Antibodies with altered effector functions
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
JP2008511337A (ja) * 2004-09-02 2008-04-17 ジェネンテック・インコーポレーテッド ヘテロ多量体分子
DK2330200T3 (en) * 2004-12-23 2017-07-24 Albumedix As GENE EXPRESSION TECHNIQUE
CN101180308A (zh) * 2005-05-23 2008-05-14 韩国科学技术院 利用sHSPs制备目的蛋白的方法
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
CA2671264C (en) 2006-11-30 2015-11-24 Research Development Foundation Improved immunoglobulin libraries
CN101294164B (zh) * 2007-04-23 2013-01-16 南京大学 重组人中期因子(rh-Midkine)的表达及其单克隆抗体的制备和应用
EP2155789B1 (en) * 2007-05-01 2013-07-24 Research Development Foundation Immunoglobulin fc libraries
CN101307310B (zh) * 2007-05-15 2012-08-08 康泰生医科技股份有限公司 具有提高产率及免疫原性的重组蛋白质表达系统
CA2766065C (en) * 2009-06-30 2020-07-21 Research Development Foundation Immunoglobulin fc polypeptides
AU2012214643B2 (en) 2011-02-07 2016-12-15 Research Development Foundation Engineered immunoglobulin Fc polypeptides
JP6411891B2 (ja) * 2012-10-03 2018-10-24 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
KR20180049238A (ko) 2012-12-05 2018-05-10 솔라 바이오사이언시즈 엘엘씨 단백질 발현 증진 폴리펩타이드
JP6724023B2 (ja) 2015-02-09 2020-07-15 リサーチ ディベロップメント ファウンデーション 改善された補体活性化を示す操作された免疫グロブリンｆｃポリペプチド
CN105296651B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp90基因表达的方法
CN105296652B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp40基因表达的方法
CN105296654B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp60基因表达的方法
CN105296653B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp83基因表达的方法
CN110734480B (zh) * 2019-11-07 2021-08-24 中国科学院遗传与发育生物学研究所 大肠杆菌分子伴侣GroEL/ES在协助合成植物Rubisco中的应用
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
CN112239760B (zh) * 2020-09-18 2022-02-11 深圳科兴药业有限公司 高效表达重组hGH的重组工程菌及构建方法和应用
CN112940955B (zh) * 2021-04-18 2022-10-18 江南大学 一种高效合成乳铁蛋白的毕赤酵母及其构建方法与应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757013A (en) * 1983-07-25 1988-07-12 The Research Foundation Of State University Of New York Cloning vehicles for polypeptide expression in microbial hosts
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
DE3611817A1 (de) 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
JP2980626B2 (ja) * 1988-01-11 1999-11-22 ゾーマ・コーポレーション ペクチン酸リアーゼのシグナル配列を含む新規プラスミドベクター
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
JPH09173078A (ja) * 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
AU692728B2 (en) * 1996-03-28 1998-06-11 Mitsui Chemicals, Inc. Method for secretory production of protein
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
JP3344618B2 (ja) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド
EP1048732A1 (de) 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
FI109361B (fi) * 1999-05-04 2002-07-15 Korpela Timo Mikrobinen proteiinin ilmentämisjärjestelmä

Also Published As

Publication number Publication date
ATE299942T1 (de) 2005-08-15
CN1231595C (zh) 2005-12-14
KR100378325B1 (ko) 2003-03-29
US6455279B1 (en) 2002-09-24
DK1077262T3 (da) 2005-11-07
EP1077262A1 (en) 2001-02-21
CA2313248A1 (en) 2001-01-29
ES2247990T3 (es) 2006-03-16
MXPA00007339A (es) 2002-08-06
EP1077263A1 (de) 2001-02-21
DE60021318D1 (de) 2005-08-25
KR20010049895A (ko) 2001-06-15
JO2309B1 (en) 2005-09-12
JP2001061487A (ja) 2001-03-13
EP1077262B1 (en) 2005-07-20
CA2313248C (en) 2010-05-25
CN1283702A (zh) 2001-02-14
DE60021318T2 (de) 2006-05-24

Similar Documents

Publication Publication Date Title
IN190327B (ko)
EP0799317B8 (en) Method for secreting thrombopoietin polypeptides
HUP0001650A2 (hu) Javított expressziós vektorok
WO1998022574A3 (de) Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
IL142269A0 (en) Process for recovering heterologous polypetides from bacterial cells
AU1550297A (en) Amino acid-enriched plant protein reserves, particularly lysine-enriched maize gamma-zein, and plants expressing such proteins
EP0737747A3 (de) Cytoplasmatische Expression von Antikörpern, Antikörperfragmenten und Antikörperfragment-Fusionsmolekülen in E. coli
MY127575A (en) Methods of synthesizing insulin polypeptide-oligomer conjugates, and proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
NZ508863A (en) Generation of multiple gene products from chimeric fusion proteins by cleavage with ubiquitous endoproteases
EP1054063A3 (en) Process for the production of naturally folded and secreted proteins
AU7057098A (en) Improved prokaryotic expression of protein
GR3034704T3 (en) Recombinant vector for the exocellular preparation of single chain antibodies expressed in bacillus subtilis.
DE69634757D1 (de) Vektor zur expression von n-terminal verlängerten proteinen in hefezellen
WO1999002708A8 (en) Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein
GB2357769A (en) Peptidyl prolyl cis-trans isomerases
EP0750040A3 (en) Macrophage stimulating protein variant and method for producing the same
AU2789000A (en) Sequences for improving the efficiency of secretion of non-secreted proteins from mammalian and insect cells
EP1517913A4 (en) A MYCOBACTERIUM TUBERCULOSIS ENCODING SEQUENCE FOR THE EXPRESSION OF HETEROLOGICAL PROTEINS
WO1996036722A3 (en) Embedded expression of heterologous proteins
EP1217006A3 (en) Polypeptides and nucleic acids encoding the same