IL73568A - Yeast hybrid vector comprising the yeast pho5 promoter,a dna sequence encoding a signal peptide,the human tissue plaminogen activator coding region and a dna sequence encoding yeast transcription termination signals,a method for producing the vector,a yeast host transformed by the vector,a method for producing the transformed host,a method for producing biologically active human tissue plasminogen activator using a so transformed host and human tissue plasminogen activator produced by the method - Google Patents
Yeast hybrid vector comprising the yeast pho5 promoter,a dna sequence encoding a signal peptide,the human tissue plaminogen activator coding region and a dna sequence encoding yeast transcription termination signals,a method for producing the vector,a yeast host transformed by the vector,a method for producing the transformed host,a method for producing biologically active human tissue plasminogen activator using a so transformed host and human tissue plasminogen activator produced by the methodInfo
- Publication number
- IL73568A IL73568A IL73568A IL7356884A IL73568A IL 73568 A IL73568 A IL 73568A IL 73568 A IL73568 A IL 73568A IL 7356884 A IL7356884 A IL 7356884A IL 73568 A IL73568 A IL 73568A
- Authority
- IL
- Israel
- Prior art keywords
- yeast
- human tissue
- producing
- vector
- sequence encoding
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title 4
- 238000004519 manufacturing process Methods 0.000 title 3
- 108091028043 Nucleic acid sequence Proteins 0.000 title 2
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 title 2
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 title 2
- 229960000187 tissue plasminogen activator Drugs 0.000 title 2
- 108091026890 Coding region Proteins 0.000 title 1
- 101150012394 PHO5 gene Proteins 0.000 title 1
- 108010076504 Protein Sorting Signals Proteins 0.000 title 1
- 239000012190 activator Substances 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 230000005030 transcription termination Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB838331045A GB8331045D0 (en) | 1983-11-21 | 1983-11-21 | Synthesis of fibrinolytic agents by yeast |
| GB848423112A GB8423112D0 (en) | 1983-11-21 | 1984-09-13 | Synthesis of fibrino lytic agents by yeast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL73568A0 IL73568A0 (en) | 1985-02-28 |
| IL73568A true IL73568A (en) | 1992-07-15 |
Family
ID=26287024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL73568A IL73568A (en) | 1983-11-21 | 1984-11-20 | Yeast hybrid vector comprising the yeast pho5 promoter,a dna sequence encoding a signal peptide,the human tissue plaminogen activator coding region and a dna sequence encoding yeast transcription termination signals,a method for producing the vector,a yeast host transformed by the vector,a method for producing the transformed host,a method for producing biologically active human tissue plasminogen activator using a so transformed host and human tissue plasminogen activator produced by the method |
Country Status (17)
| Country | Link |
|---|---|
| EP (1) | EP0143081B1 (pt) |
| JP (1) | JPS6119489A (pt) |
| KR (1) | KR930002889B1 (pt) |
| AU (1) | AU595478B2 (pt) |
| DD (1) | DD229151A5 (pt) |
| DE (1) | DE3479520D1 (pt) |
| DK (1) | DK164941C (pt) |
| ES (1) | ES8609471A1 (pt) |
| FI (1) | FI86746C (pt) |
| GR (1) | GR80966B (pt) |
| HU (1) | HU201115B (pt) |
| IE (1) | IE57699B1 (pt) |
| IL (1) | IL73568A (pt) |
| NO (1) | NO844618L (pt) |
| NZ (1) | NZ210266A (pt) |
| PH (1) | PH22337A (pt) |
| PT (1) | PT79518B (pt) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR78180B (pt) * | 1982-04-15 | 1984-09-26 | Genentech Inc | |
| US7138505B1 (en) | 1984-01-12 | 2006-11-21 | Novartis Vaccines And Diagnostics, Inc. | Factor VIII:C nucleic acid molecules |
| JPS61502655A (ja) * | 1984-07-09 | 1986-11-20 | インテグレ−テッド・ジェネティックス・インコ−ポレ−テッド | 酵母クロ−ニングビヒクル |
| NZ213647A (en) * | 1984-10-01 | 1989-05-29 | Integrated Genetics Inc | Recombinant human tissue plasminogen activator (tpa) modified in n-linked glycosylation pattern |
| JPS624233A (ja) * | 1985-07-01 | 1987-01-10 | Toyobo Co Ltd | 組織性プラスミノ−ゲン活性化因子の製造法 |
| JPS6229975A (ja) * | 1985-08-01 | 1987-02-07 | Mitsui Toatsu Chem Inc | 粗ｔＰＡの精製法 |
| GB8521496D0 (en) * | 1985-08-29 | 1985-10-02 | Ciba Geigy Ag | Repressible yeast promoters |
| EP0216573B1 (en) * | 1985-09-18 | 1990-11-22 | Green Cross Corporation | Yeast promoter and method of producing heterologous proteins |
| USH2055H1 (en) * | 1985-09-20 | 2002-12-03 | Zymogenetics, Inc. | Expression of tissue-type plasminogen activator in bacteria |
| GB8530631D0 (en) | 1985-12-12 | 1986-01-22 | Ciba Geigy Ag | Thrombin inhibitors |
| MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
| US5106741A (en) * | 1985-12-20 | 1992-04-21 | The Upjohn Company | Tissue plasminogen activator (TPA) analogs |
| US4898825A (en) * | 1986-05-07 | 1990-02-06 | Mitsui Toatsu Chemicals, Incorporated | Methods for purification of single-chain and double-chain tissue plasminogen activator |
| ZA872534B (en) * | 1986-05-08 | 1987-11-25 | Phillips Petroleum Co | Yeast production of streptokinase |
| PT84991B (pt) * | 1986-06-06 | 1990-03-08 | Genentech Inc | Processo para a producao de actividador do plasminogenio biologicamente activo |
| JPS6463378A (en) * | 1986-08-11 | 1989-03-09 | Mitsui Toatsu Chemicals | Separation of single stranded tpa and double standard tpa |
| GB8621118D0 (en) * | 1986-09-01 | 1986-10-08 | Whitbread & Co Plc | Stabilised polypeptides |
| DE3789413T2 (de) | 1986-10-03 | 1994-07-28 | Ciba Geigy Ag | Lymphokin-ähnliche Peptide. |
| PT86162B (pt) * | 1986-11-21 | 1990-11-20 | Smithkline Beecham Corp | Expressao de proteina recombinante |
| ZA879286B (en) | 1986-12-16 | 1988-10-26 | Smith Kline Rit | New plasminogen activators |
| US5149533A (en) * | 1987-06-04 | 1992-09-22 | Zymogenetics, Inc. | Modified t-PA with kringle-/replaced by another kringle |
| US5089409A (en) * | 1989-09-28 | 1992-02-18 | Monsanto Company | Method of increasing specific activity of t-pa |
| US5326700A (en) * | 1990-11-06 | 1994-07-05 | Eli Lilly And Company | DNA sequences encoding t-PA derivatives with cleavable sites |
| WO1995004823A1 (en) * | 1993-08-04 | 1995-02-16 | Ciba-Geigy Ag | High molecular weight desulphatohirudin |
| PT801682E (pt) | 1995-02-09 | 2002-03-28 | Behringwerke Ag | Processo para a producao de proteinas |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
| AR241654A1 (es) * | 1982-05-05 | 1992-10-30 | Genentech Inc | Procedimiento para producir activador de plasminogeno de tejido humano. |
| GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
| EP0174835A1 (en) * | 1984-09-11 | 1986-03-19 | The Upjohn Company | Human tissue plasminogen activator and recombinant DNA compounds |
| NZ213647A (en) * | 1984-10-01 | 1989-05-29 | Integrated Genetics Inc | Recombinant human tissue plasminogen activator (tpa) modified in n-linked glycosylation pattern |
-
1984
- 1984-11-19 PT PT79518A patent/PT79518B/pt not_active IP Right Cessation
- 1984-11-19 DE DE8484810564T patent/DE3479520D1/de not_active Expired
- 1984-11-19 GR GR80966A patent/GR80966B/el unknown
- 1984-11-19 DD DD84269628A patent/DD229151A5/de not_active IP Right Cessation
- 1984-11-19 PH PH31460A patent/PH22337A/en unknown
- 1984-11-19 EP EP84810564A patent/EP0143081B1/en not_active Expired
- 1984-11-19 FI FI844544A patent/FI86746C/fi not_active IP Right Cessation
- 1984-11-20 NO NO844618A patent/NO844618L/no unknown
- 1984-11-20 NZ NZ210266A patent/NZ210266A/xx unknown
- 1984-11-20 IE IE2965/84A patent/IE57699B1/en not_active IP Right Cessation
- 1984-11-20 DK DK551084A patent/DK164941C/da not_active IP Right Cessation
- 1984-11-20 HU HU844309A patent/HU201115B/hu not_active IP Right Cessation
- 1984-11-20 AU AU35719/84A patent/AU595478B2/en not_active Ceased
- 1984-11-20 IL IL73568A patent/IL73568A/xx unknown
- 1984-11-20 JP JP59243529A patent/JPS6119489A/ja active Pending
- 1984-11-21 KR KR1019840007284A patent/KR930002889B1/ko not_active Expired - Lifetime
- 1984-11-21 ES ES537828A patent/ES8609471A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DD229151A5 (de) | 1985-10-30 |
| DK551084D0 (da) | 1984-11-20 |
| NZ210266A (en) | 1988-11-29 |
| KR850003733A (ko) | 1985-06-26 |
| EP0143081A2 (en) | 1985-05-29 |
| JPS6119489A (ja) | 1986-01-28 |
| KR930002889B1 (ko) | 1993-04-12 |
| FI844544A0 (fi) | 1984-11-19 |
| IL73568A0 (en) | 1985-02-28 |
| AU3571984A (en) | 1986-03-20 |
| PT79518A (en) | 1984-12-01 |
| HUT36185A (en) | 1985-08-28 |
| NO844618L (no) | 1985-05-22 |
| DK551084A (da) | 1985-05-22 |
| FI86746B (fi) | 1992-06-30 |
| ES537828A0 (es) | 1986-07-16 |
| ES8609471A1 (es) | 1986-07-16 |
| EP0143081A3 (en) | 1987-01-07 |
| HU201115B (en) | 1990-09-28 |
| FI844544L (fi) | 1985-05-22 |
| IE842965L (en) | 1985-05-21 |
| FI86746C (fi) | 1992-10-12 |
| PH22337A (en) | 1988-08-12 |
| DE3479520D1 (en) | 1989-09-28 |
| DK164941C (da) | 1993-02-01 |
| PT79518B (pt) | 1986-12-12 |
| IE57699B1 (en) | 1993-03-10 |
| AU595478B2 (en) | 1990-04-05 |
| DK164941B (da) | 1992-09-14 |
| EP0143081B1 (en) | 1989-08-23 |
| GR80966B (en) | 1985-03-15 |
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