IL225109A - Direct capture, amplification and arrangement of target DNA using bled primers - Google Patents

Direct capture, amplification and arrangement of target DNA using bled primers

Info

Publication number
IL225109A
IL225109A IL225109A IL22510913A IL225109A IL 225109 A IL225109 A IL 225109A IL 225109 A IL225109 A IL 225109A IL 22510913 A IL22510913 A IL 22510913A IL 225109 A IL225109 A IL 225109A
Authority
IL
Israel
Prior art keywords
sequence
primer
seq
adaptor
genomic
Prior art date
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IL225109A
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English (en)
Hebrew (he)
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Univ Leland Stanford Junior
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Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of IL225109A publication Critical patent/IL225109A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
IL225109A 2010-09-24 2013-03-07 Direct capture, amplification and arrangement of target DNA using bled primers IL225109A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38639010P 2010-09-24 2010-09-24
US201161485062P 2011-05-11 2011-05-11
PCT/US2011/052645 WO2012040387A1 (en) 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers

Publications (1)

Publication Number Publication Date
IL225109A true IL225109A (en) 2017-05-29

Family

ID=45874160

Family Applications (1)

Application Number Title Priority Date Filing Date
IL225109A IL225109A (en) 2010-09-24 2013-03-07 Direct capture, amplification and arrangement of target DNA using bled primers

Country Status (13)

Country Link
US (3) US9309556B2 (https=)
EP (2) EP2619329B1 (https=)
JP (1) JP5986572B2 (https=)
KR (1) KR20130113447A (https=)
CN (1) CN103228798B (https=)
AU (1) AU2011305445B2 (https=)
CA (1) CA2810931C (https=)
IL (1) IL225109A (https=)
IN (1) IN2013MN00522A (https=)
MX (1) MX346956B (https=)
NZ (1) NZ608313A (https=)
RU (1) RU2565550C2 (https=)
WO (1) WO2012040387A1 (https=)

Families Citing this family (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
EP4372084A3 (en) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP4026912A1 (en) 2012-05-10 2022-07-13 The General Hospital Corporation Methods for determining a nucleotide sequence
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
AU2013292610B2 (en) * 2012-07-17 2018-05-17 Myriad Women's Health, Inc. System and methods for detecting genetic variation
CN103571822B (zh) * 2012-07-20 2016-03-30 中国科学院植物研究所 一种用于新一代测序分析的多重目的dna片段富集方法
GB2533006B (en) * 2012-09-04 2017-06-07 Guardant Health Inc Systems and methods to detect copy number variation
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP4293125A3 (en) * 2012-12-10 2024-02-28 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN105339507A (zh) * 2013-02-21 2016-02-17 托马生物科学公司 用于核酸分析的方法、组合物和试剂盒
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
US11049587B2 (en) 2013-10-18 2021-06-29 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
AU2014337093B2 (en) 2013-10-18 2020-07-30 Seven Bridges Genomics Inc. Methods and systems for identifying disease-induced mutations
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
WO2015058095A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for quantifying sequence alignment
JP2017500004A (ja) 2013-10-18 2017-01-05 セブン ブリッジズ ジェノミクス インコーポレイテッド 遺伝子試料について遺伝子型解析するための方法およびシステム
US9063914B2 (en) 2013-10-21 2015-06-23 Seven Bridges Genomics Inc. Systems and methods for transcriptome analysis
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN111118125B (zh) * 2013-11-26 2023-06-30 杭州联川基因诊断技术有限公司 一种纯化pcr产物的方法
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
CN105793438B (zh) * 2013-12-15 2020-02-11 中央研究院 未知序列的双股线性核酸的全长扩增方法
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
US9677132B2 (en) * 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
HK1225758A1 (zh) 2014-01-16 2017-09-15 Illumina, Inc. 固体支持物上的扩增子制备和测序
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
WO2015131107A1 (en) * 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
US10570447B2 (en) * 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
US20150361422A1 (en) * 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
EP3919621A1 (en) 2014-06-23 2021-12-08 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
CA2964349C (en) 2014-10-14 2023-03-21 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
EP4095261B1 (en) 2015-01-06 2025-05-28 Molecular Loop Biosciences, Inc. Screening for structural variants
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
WO2016196827A1 (en) * 2015-06-02 2016-12-08 Biofire Defense, Llc Sample to sequence
RU2604198C1 (ru) * 2015-06-22 2016-12-10 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Способ получения гетерогенного набора одноцепочечных фрагментов днк для мультиплексного генетического анализа
ES2888626T3 (es) * 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
AU2016321328A1 (en) 2015-09-09 2018-04-26 Psomagen, Inc. Method and system for microbiome-derived diagnostics and therapeutics infectious disease and other health conditions associated with antibiotic usage
KR102753113B1 (ko) 2015-09-18 2025-01-09 바나디스 다이아그노스틱스 Dna 샘플의 분석을 위한 프로브 세트 및 이의 사용 방법
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
KR101651817B1 (ko) * 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
RU2018121254A (ru) 2015-11-11 2019-12-16 Резолюшн Байосайенс, Инк. Высокоэффективное построение библиотек днк
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017168331A1 (en) * 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex fragment sequencing
WO2017193044A1 (en) 2016-05-06 2017-11-09 Counsyl, Inc. Noninvasive prenatal diagnostic
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018017884A1 (en) 2016-07-20 2018-01-25 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
WO2018053362A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
EP3518974A4 (en) 2016-09-29 2020-05-27 Myriad Women's Health, Inc. NON-INVASIVE PRENATAL SCREENING USING DYNAMIC ITERATIVE DEEP OPTIMIZATION
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
US10844429B2 (en) 2017-01-18 2020-11-24 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10752946B2 (en) 2017-01-31 2020-08-25 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
EP3602359A4 (en) 2017-03-24 2021-01-06 Myriad Women's Health, Inc. COPY NUMBER VARIANT CALLER
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019060628A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
CN111542532B (zh) * 2017-10-04 2024-03-15 生捷科技控股公司 酶法合成寡核苷酸的方法和体系
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111788316B (zh) * 2018-02-07 2024-11-29 帝肯基因组学公司 库制备
CA3097044A1 (en) 2018-04-17 2019-10-24 The General Hospital Corporation Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents
CN120624607A (zh) 2018-05-08 2025-09-12 深圳华大智造科技股份有限公司 用于准确且经济高效的测序、单体型分型和组装的基于单管珠粒的dna共条形码化
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
WO2020039261A1 (en) 2018-08-23 2020-02-27 Boreal Genomics, Inc. Linked target capture and ligation
WO2020076914A1 (en) * 2018-10-09 2020-04-16 Academia Sinica Digital polymerase chain reaction method for detecting nucleic acids in samples
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US12247198B2 (en) 2018-12-14 2025-03-11 Bgi Shenzhen Nucleic acid synthesis and purification device, use thereof, and nucleic acid synthesis and purification method
CN112654714B (zh) * 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
WO2020141464A1 (en) 2019-01-03 2020-07-09 Boreal Genomics, Inc. Linked target capture
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US20220228139A1 (en) * 2019-05-21 2022-07-21 Academia Sinica Method for amplifying and detecting ribonucleic acid (rna) fragments
US11639521B2 (en) * 2019-06-03 2023-05-02 The Chinese University Of Hong Kong Method for determining the copy number of a tandem repeat sequence
CN112342269B (zh) * 2019-08-09 2023-12-05 深圳市真迈生物科技有限公司 一种捕获核酸分子的方法及其应用
US11795495B1 (en) * 2019-10-02 2023-10-24 FOXO Labs Inc. Machine learned epigenetic status estimator
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
EP4097231B1 (en) * 2020-01-31 2025-03-19 Agilent Technologies, Inc. Systems and methods for targeted nucleic acid capture
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
MX2021015806A (es) * 2020-02-26 2022-04-27 Illumina Inc Kits para genotipificacion.
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150126A4 (en) * 2020-05-12 2024-06-19 Singular Genomics Systems, Inc. NUCLEIC ACID AMPLIFICATION PROCESSES
CN111676276A (zh) * 2020-07-13 2020-09-18 湖北伯远合成生物科技有限公司 一种快速精准确定基因编辑突变情况的方法及其应用
EP4153606A4 (en) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES
US20230212560A1 (en) * 2020-07-31 2023-07-06 Arc Bio, Llc Systems, methods, and media for determining relative quality of oligonucleotide preparations
WO2022055969A1 (en) * 2020-09-08 2022-03-17 Seqwell, Inc. Sequencing oligonucleotides and methods of use thereof
US12499970B2 (en) 2020-09-30 2025-12-16 Myriad Women's Health, Inc. Deep learning based variant calling using machine learning
RU2748380C1 (ru) * 2020-11-10 2021-05-25 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Набор олигонуклеотидных праймеров и способ генотипирования однонуклеотидного полиморфизма rs8065080 в гене TRPV1 человека
CN112837749B (zh) * 2021-02-01 2021-11-26 北京百奥纳芯生物科技有限公司 一种癌症筛查用基因芯片探针的优选方法
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
JP2024528704A (ja) * 2021-07-20 2024-07-30 フリーノム ホールディングス,インク. 核酸シーケンシングにおける、改善された5-ヒドロキシメチル化シトシンの分解能のための組成物および方法
CN114621307B (zh) * 2022-04-12 2024-12-13 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
WO2025072683A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification

Family Cites Families (271)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
AU4128089A (en) 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US7223833B1 (en) 1991-05-24 2007-05-29 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
ATE198358T1 (de) 1992-04-27 2001-01-15 Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CH686982A5 (fr) 1993-12-16 1996-08-15 Maurice Stroun Méthode pour le diagnostic de cancers.
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5789206A (en) 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
NO954667D0 (no) 1995-11-17 1995-11-17 Dagfinn Oegreid Fremgangsmåte til deteksjon av Ki-ras mutasjoner
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
JP2000505312A (ja) 1996-03-18 2000-05-09 モレキュラー バイオロジー リソーシーズ,インコーポレイテッド 標的核酸配列増幅
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
SE9602829D0 (sv) 1996-07-19 1996-07-19 Yngve Johnsson Förfarande och anordning för betalningsmedelshantering jämte betalningsmedel
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6482795B1 (en) 1997-01-30 2002-11-19 Myriad Genetics, Inc. Tumor suppressor designated TS10q23.3
US6262242B1 (en) 1997-01-30 2001-07-17 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JPH10341047A (ja) 1997-06-06 1998-12-22 Sony Corp 磁気トンネル素子
JP4298912B2 (ja) 1997-07-07 2009-07-22 メディカル リサーチ カウンシル invitro選別法
US6008002A (en) 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
WO1999041613A1 (en) 1998-02-12 1999-08-19 Immunivest Methods and reagents for the rapid and efficient isolation of circulating cancer cells
JPH11355695A (ja) 1998-06-10 1999-12-24 Sony Corp 映像信号処理装置
DE19833738A1 (de) 1998-07-27 2000-02-03 Michael Giesing Verfahren zur Isolierung von Krebszellen aus zellhaltigen Körperflüssigkeiten sowie Sets zur Durchführung dieses Verfahrens
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
WO2000040758A2 (en) 1999-01-06 2000-07-13 Hyseq Inc. Enhanced sequencing by hybridization using pools of probes
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US9534254B1 (en) 1999-02-02 2017-01-03 Abbott Molecular Inc. Patient stratification for cancer therapy based on genomic DNA microarray analysis
US6395481B1 (en) 1999-02-16 2002-05-28 Arch Development Corp. Methods for detection of promoter polymorphism in a UGT gene promoter
US6410231B1 (en) 1999-02-26 2002-06-25 Incyte Genomics, Inc. SNP detection
US7324926B2 (en) 1999-04-09 2008-01-29 Whitehead Institute For Biomedical Research Methods for predicting chemosensitivity or chemoresistance
US20010034023A1 (en) 1999-04-26 2001-10-25 Stanton Vincent P. Gene sequence variations with utility in determining the treatment of disease, in genes relating to drug processing
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
JP2003508066A (ja) 1999-09-01 2003-03-04 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ タンパク質−dna相互作用の染色体全体の解析
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
AU2001231091A1 (en) 2000-01-21 2001-07-31 Vincent P. Stanton Jr. Identification of genetic components of drug response
WO2001055720A2 (en) 2000-01-26 2001-08-02 Ventana Medical Systems, Inc. A system for developing assays for personalized medicine
WO2001064864A2 (en) 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
EP1995330A1 (en) 2000-03-01 2008-11-26 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US20020120409A1 (en) 2000-05-19 2002-08-29 Affymetrix, Inc. Methods for gene expression analysis
EP1297004A2 (en) 2000-06-15 2003-04-02 Prokaria ehf. Thermostable cellulase
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7105293B2 (en) 2000-09-19 2006-09-12 Whitehead Institute For Biomedical Research Genetic markers for tumors
DE60131903T2 (de) 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
US20050021240A1 (en) 2000-11-02 2005-01-27 Epigenomics Ag Systems, methods and computer program products for guiding selection of a therapeutic treatment regimen based on the methylation status of the DNA
AU2002218006A1 (en) 2000-11-03 2002-05-15 Dana Farber Cancer Institute Compositions and methods for the diagnosis of cancer
EP1207209A3 (en) 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
CA2436910C (en) 2000-12-01 2014-06-17 Response Genetics, Inc. Method of determining epidermal growth factor receptor and her2-neu gene expression and correlation of levels thereof with survival rates
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US20020115073A1 (en) 2001-02-16 2002-08-22 Nickolas Papadopoulos Genome-based personalized medicine
DE60238085D1 (de) 2001-02-23 2010-12-02 Japan Science & Tech Agency Vorrichtung und Verfahren zum herstellen von Mikrokapseln
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20040110193A1 (en) 2001-07-31 2004-06-10 Gene Logic, Inc. Methods for classification of biological data
US20030165940A1 (en) 2001-12-06 2003-09-04 The Johns Hopkins University Disease detection by digital protein truncation assays
AU2002359645A1 (en) 2001-12-07 2003-07-09 University Of Massachusetts Targeted genetic risk-stratification using microarrays
NZ532780A (en) 2001-12-21 2006-10-27 Wellcome Trust Isolated naturally occurring mutant human B-Raf polypeptides and nucleic acids encoding them
US6949342B2 (en) 2001-12-21 2005-09-27 Whitehead Institute For Biomedical Research Prostate cancer diagnosis and outcome prediction by expression analysis
US7730063B2 (en) 2002-12-10 2010-06-01 Asset Trust, Inc. Personalized medicine service
US7473534B2 (en) 2002-03-01 2009-01-06 Siemens Healthcare Diagnostics Inc. Assays for cancer patient monitoring based on levels of epidermal growth factor receptor (EGFR) extracellular domain (ECD) analyte, alone or in combination with other analytes, in body fluid samples
AU2003213836A1 (en) 2002-03-11 2003-09-29 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7427480B2 (en) 2002-03-26 2008-09-23 Perlegen Sciences, Inc. Life sciences business systems and methods
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
AU2003249681A1 (en) * 2002-05-31 2003-12-19 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US9388459B2 (en) * 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
TWI266907B (en) 2002-07-24 2006-11-21 Nitto Denko Corp Polarizing device, optical thin film using the same, and image display device using the same
US7153658B2 (en) 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
ATE391173T1 (de) 2002-09-20 2008-04-15 Prokaria Ehf Thermostabile ligase aus thermus phage
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
AU2003295598B2 (en) 2002-11-15 2009-12-24 Genomic Health, Inc. Gene expression profiling of EGFR positive cancer
US20040224325A1 (en) 2002-12-20 2004-11-11 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US20040137539A1 (en) 2003-01-10 2004-07-15 Bradford Sherry A. Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
WO2004083460A1 (en) 2003-03-20 2004-09-30 Csi Biotech Oy Amp ligation assay (ala)
WO2004092333A2 (en) 2003-04-09 2004-10-28 Omicia Inc. Methods of selection, reporting and analysis of genetic markers using broad based genetic profiling applications
WO2004096984A2 (en) 2003-04-25 2004-11-11 Janssen Pharmaceutica N.V. Preservation of rna in a biological sample
WO2004111603A2 (en) 2003-05-28 2004-12-23 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
RU2392324C2 (ru) * 2003-09-18 2010-06-20 Симфоген А/С Способ связывания интересующих последовательностей
CA2543786A1 (en) 2003-10-24 2005-05-06 Mmi Genomics, Inc. Methods and systems for inferring traits to manage non-beef livestock
JP4942484B2 (ja) 2003-10-28 2012-05-30 エポック・バイオサイエンシーズ・インコーポレイテッド 改良された感度および低バックグラウンドを備えるハイブリダイゼーションによるdna検出のための蛍光プローブ
EP1756137A4 (en) 2003-11-05 2007-10-31 Univ Texas DIAGNOSTIC AND THERAPEUTIC PROCEDURES AND COMPOSITIONS CONCERNING PTEN AND BREAST CANCER
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US20050181377A1 (en) 2004-02-13 2005-08-18 Markovic Svetomir N. Targeted cancer therapy
US20060195266A1 (en) 2005-02-25 2006-08-31 Yeatman Timothy J Methods for predicting cancer outcome and gene signatures for use therein
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20070275386A1 (en) 2004-03-01 2007-11-29 Ratain Mark J Polymorphisms in the Epidermal Growth Factor Receptor Gene Promoter
ATE399884T1 (de) 2004-03-24 2008-07-15 Applera Corp Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
CN101501211A (zh) 2004-03-31 2009-08-05 综合医院公司 测定癌症对表皮生长因子受体靶向性治疗反应性的方法
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
CA2563074C (en) 2004-04-09 2014-05-20 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
US20090246756A1 (en) 2004-05-06 2009-10-01 Prokaria Ehe Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP1766068A4 (en) 2004-06-04 2010-03-03 Genentech Inc EGFR Mutations
US7329495B2 (en) 2004-06-09 2008-02-12 Board Of Regents, The University Of Texas System Mutations in KIT confer imatinib resistance in gastrointestinal stromal tumors
US20060115827A1 (en) 2004-07-01 2006-06-01 University Of Southern California Genetic markers for predicting disease and treatment outcome
US8298756B2 (en) 2004-08-11 2012-10-30 Albert Einstein College Of Medicine Of Yeshiva University Isolation, gene expression, and chemotherapeutic resistance of motile cancer cells
WO2006044994A2 (en) 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
KR20070073917A (ko) 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 개선된 증폭을 위한 핵산 복구
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US20060278241A1 (en) 2004-12-14 2006-12-14 Gualberto Ruano Physiogenomic method for predicting clinical outcomes of treatments in patients
US20060184489A1 (en) 2004-12-17 2006-08-17 General Electric Company Genetic knowledgebase creation for personalized analysis of medical conditions
EP1851332A4 (en) 2005-01-21 2010-05-26 Gene Express Inc METHOD AND COMPOSITIONS FOR THE EVALUATION OF NUCLEIC ACIDS
US7442507B2 (en) 2005-01-24 2008-10-28 New York University School Of Medicine Methods for detecting circulating mutant BRAF DNA
EP1859330B1 (en) 2005-01-28 2012-07-04 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US7635570B2 (en) 2005-04-01 2009-12-22 Salvatore Siena Epidermal growth factor receptor gene copy number
EP1712639B1 (en) 2005-04-06 2008-08-27 Maurice Stroun Method for the diagnosis of cancer by detecting circulating DNA and RNA
KR20080003422A (ko) 2005-04-14 2008-01-07 메르크 파텐트 게엠베하 종양 조직 내의 egfr 유전자의 증가된 복제수에 기초한항-egfr 항체 치료
JP4547301B2 (ja) 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス及び分析システム
US20070020657A1 (en) 2005-05-20 2007-01-25 Grebe Stefan K Methods for detecting circulating tumor cells
EP1885878B1 (en) 2005-05-31 2010-08-11 Dako Denmark A/S Compositions and methods for predicting outcome of treatment
PL1913157T5 (pl) 2005-06-28 2017-09-29 Genentech Inc Mutacje EGFR i KRAS do prognozowania reakcji pacjenta na leczenie inhibitorem EGFR
GB0514909D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP2009508495A (ja) 2005-09-16 2009-03-05 454 ライフ サイエンシーズ コーポレイション cDNAライブラリー調製
WO2007035842A2 (en) 2005-09-21 2007-03-29 Ccc Diagnostics, Llc Comprehensive diagnostic testing procedures for personalized anticancer chemotherapy (pac)
CA2624086A1 (en) 2005-09-28 2007-04-05 H. Lee Moffitt Cancer Center Individualized cancer treatments
EP2428579B1 (en) 2005-10-24 2013-05-29 The Johns Hopkins University Improved methods for BEAMing
CN101365803B (zh) 2005-12-22 2013-03-20 关键基因股份有限公司 采用高通量测序技术的改进的转录谱描述策略
WO2007091230A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A microfluidic analysis system
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
EP1981624B1 (en) 2006-02-07 2011-09-07 Stokes Bio Limited A liquid bridge system and method
ATE483972T1 (de) 2006-02-16 2010-10-15 Ventana Med Syst Inc Reagenzien und verfahren für krebsprognose und pathologische einstufung
CN101395280A (zh) 2006-03-01 2009-03-25 凯津公司 基于测序的高通量SNPs连接检测技术
US8165367B2 (en) 2006-03-08 2012-04-24 Olympus Medical Systems Corp. Medical image processing apparatus and medical image processing method having three-dimensional model estimating
WO2007106432A2 (en) 2006-03-10 2007-09-20 George Mason Intellectual Properties, Inc. Egf receptor phosphorylation status for disease treatment
US7908091B2 (en) 2006-03-17 2011-03-15 Prometheus Laboratories Inc. Methods of predicting and monitoring tyrosine kinase inhibitor therapy
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
BRPI0709340A2 (pt) 2006-03-27 2013-04-16 Globeimmune, Inc. mutaÇço ras e composiÇÕes e mÉtodos de uso da mesma
TWI395754B (zh) 2006-04-24 2013-05-11 Amgen Inc 人類化之c-kit抗體
US8380539B2 (en) 2006-05-09 2013-02-19 University Of Louisville Research Foundation, Inc. Personalized medicine management software
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
CN103424541B (zh) 2006-05-18 2018-01-30 分子压型学会股份有限公司 确定针对病状的个性化医疗介入的系统和方法
US20080090239A1 (en) 2006-06-14 2008-04-17 Daniel Shoemaker Rare cell analysis using sample splitting and dna tags
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US20080065411A1 (en) 2006-09-08 2008-03-13 Diaceutics Method and system for developing a personalized medicine business plan
KR20090071603A (ko) 2006-09-19 2009-07-01 노파르티스 아게 Raf 억제제에 대한 표적 조절, 효능, 진단 및/또는 예후의 바이오마커
WO2008038259A1 (en) 2006-09-28 2008-04-03 Stokes Bio Limited A qpcr analysis apparatus
EP2076609A1 (en) 2006-10-10 2009-07-08 Illumina Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
JP5631000B2 (ja) 2006-10-27 2014-11-26 デコード・ジェネティクス・イーエイチエフ Chr8q24.21上の癌感受性変異体
JP2010508277A (ja) 2006-11-01 2010-03-18 ジョージ メイソン インテレクチュアル プロパティーズ,インコーポレイテッド オブ フェアファックス,バージニア 癌を検出および抑制するための方法
US20100196889A1 (en) 2006-11-13 2010-08-05 Bankaitis-Davis Danute M Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer
US20080131887A1 (en) 2006-11-30 2008-06-05 Stephan Dietrich A Genetic Analysis Systems and Methods
WO2008115300A1 (en) 2006-12-01 2008-09-25 Apocell, Inc. C-kit phosphorylation in cancer
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20080177608A1 (en) 2007-01-19 2008-07-24 Diaceutics Business method for enabling personalized medicine
JP2010528585A (ja) 2007-02-26 2010-08-26 ジョン ウェイン キャンサー インスティテュート 癌の診断および治療におけるb−rafdna変異の有用性
US20100166747A1 (en) 2007-03-02 2010-07-01 Beltran Pedro J Methods and compositions for treating tumor diseases
ES2426814T3 (es) 2007-03-13 2013-10-25 Amgen Inc. Mutaciones de K-ras y B-raf y terapia con anticuerpos anti-EGFr
AR065687A1 (es) 2007-03-13 2009-06-24 Amgen Inc Metodo para determinar la presencia o no de una mutacion k-ras y terapia con anticuerpos anti-egfr
US20090258795A1 (en) 2007-03-15 2009-10-15 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
US20080242622A1 (en) 2007-03-19 2008-10-02 Cold Spring Harbor Laboratory Identification of genetic alterations that modulate drug sensitivity in cancer treatments
US20080255243A1 (en) 2007-04-13 2008-10-16 Petricoin Emanuel F Stat3 as a theranostic indicator
US20100255004A1 (en) 2007-04-13 2010-10-07 Dana Farber Cancer Institute Receptor tyrosine kinase profiling
WO2008147879A1 (en) 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
WO2008148072A2 (en) 2007-05-24 2008-12-04 The Brigham And Women's Hospital, Inc. Disease-associated genetic variations and methods for obtaining and using same
ES2959127T3 (es) 2007-06-19 2024-02-20 Hoffmann La Roche Secuenciación de ácidos nucleicos de alto rendimiento por expansión
GB0712882D0 (en) * 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
ES2526211T3 (es) 2007-07-13 2015-01-08 Nestec S.A. Selección de fármacos para la terapia del cáncer de pulmón utilizando matrices basadas en anticuerpos
LT2557517T (lt) 2007-07-23 2023-01-10 The Chinese University Of Hong Kong Nukleino rūgščių sekos disbalanso nustatymas
US8043815B2 (en) 2007-08-06 2011-10-25 Health Research, Inc. Methods for analysis of PDEF and survivin as interconnected cancer biomarkers and targets for personalized medicine
US20090062138A1 (en) 2007-08-31 2009-03-05 Curry Bo U Array-based method for performing SNP analysis
US20100173294A1 (en) 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
US20110212991A1 (en) 2007-09-11 2011-09-01 Roche Molecular Systems, Inc. Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009039202A1 (en) * 2007-09-17 2009-03-26 Twof, Inc. Hydrogel labeled primer extension method for microarrays
AU2008308457A1 (en) 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20090264298A1 (en) 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
EP2063132A1 (de) * 2007-11-24 2009-05-27 Festo AG & Co. KG Linearantriebsvorrichtung
EP2235217B1 (en) 2008-01-09 2016-04-20 Life Technologies Corporation Method of making a paired tag library for nucleic acid sequencing
ES2990227T3 (es) 2008-01-17 2024-11-29 Sequenom Inc Composiciones y procedimientos de análisis de ácido nucleico de secuencia única
US11051733B2 (en) 2008-01-18 2021-07-06 Wake Forest University Health Sciences Isolating and purifying cells for therapy
EP2245199B1 (en) 2008-02-01 2013-11-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2009099602A1 (en) 2008-02-04 2009-08-13 Massachusetts Institute Of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
AU2009213689B2 (en) 2008-02-14 2014-11-20 Decode Genetics Ehf. Susceptibility variants for lung cancer
WO2009102957A2 (en) 2008-02-14 2009-08-20 The Johns Hopkins University Methods to connect gene set expression profiles to drug sensitivity
NZ617520A (en) 2008-02-25 2015-05-29 Nestec Sa Drug selection for breast cancer therapy using antibody-based arrays
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
WO2009114836A1 (en) 2008-03-14 2009-09-17 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
US20090307179A1 (en) 2008-03-19 2009-12-10 Brandon Colby Genetic analysis
US7842248B2 (en) 2008-06-20 2010-11-30 Silverbrook Research Pty Ltd Microfluidic system comprising microfluidic pump, mixer or valve
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
JP2010041985A (ja) * 2008-08-18 2010-02-25 Hitachi Plant Technologies Ltd 核酸配列増幅方法、核酸配列の検出方法及び核酸増幅・検出用基板
US8583380B2 (en) * 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
CN102232117A (zh) 2008-10-14 2011-11-02 卡里斯Mpi公司 描绘肿瘤类型生物标志模式和特征集的基因靶和基因表达的蛋白靶
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8349563B2 (en) 2008-11-18 2013-01-08 Life Technologies Corporation Sequence amplification with target primers
WO2010059742A1 (en) 2008-11-18 2010-05-27 Collabrx, Inc. Individualized cancer treatment
US9534255B2 (en) 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US8790873B2 (en) 2009-01-16 2014-07-29 Affymetrix, Inc. DNA ligation on RNA template
CA2751470C (en) 2009-02-16 2016-07-26 Epicentre Technologies Corporation Template-independent ligation of single-stranded dna
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
WO2010127045A2 (en) 2009-04-29 2010-11-04 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
EP2494069B1 (en) * 2009-10-30 2013-10-02 Roche Diagniostics GmbH Method for detecting balanced chromosomal aberrations in a genome
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
JP2013511991A (ja) 2009-11-25 2013-04-11 クアンタライフ, インコーポレイテッド 遺伝子材料を検出する方法および組成物
US20110157322A1 (en) 2009-12-31 2011-06-30 Broadcom Corporation Controlling a pixel array to support an adaptable light manipulator
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
PL2539450T3 (pl) 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
US20130143276A1 (en) 2010-04-01 2013-06-06 New England Biolabs, Inc. Compositions and Methods for Adenylating Oligonucleotides
WO2011140510A2 (en) 2010-05-06 2011-11-10 Bioo Scientific Corporation Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing
US20110299645A1 (en) 2010-06-07 2011-12-08 Korea Hydro & Nuclear Power Co., Ltd. Breeding Nuclear Fuel Mixture Using Metallic Thorium
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
US9353406B2 (en) 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
EP3564392B1 (en) 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
KR20190002733A (ko) 2010-12-30 2019-01-08 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
DK2748357T3 (en) 2011-09-07 2018-07-16 X Chem Inc Methods for labeling DNA-encoded libraries
CA2852098C (en) 2011-10-21 2023-05-02 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
EP4372084A3 (en) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013113012A2 (en) 2012-01-27 2013-08-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
WO2013119690A1 (en) 2012-02-06 2013-08-15 Wisconsin Alumni Research Foundation Nucleic acid ligation method
WO2013173472A1 (en) 2012-05-15 2013-11-21 Predictive Biosciences, Inc. Methods of assessing chromosomal instabilities
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN105339507A (zh) 2013-02-21 2016-02-17 托马生物科学公司 用于核酸分析的方法、组合物和试剂盒
US20140287937A1 (en) 2013-02-21 2014-09-25 Toma Biosciences, Inc. Methods for assessing cancer
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
JP2019511070A (ja) 2016-02-09 2019-04-18 トマ・バイオサイエンシズ,インコーポレーテッド 核酸を解析するシステムおよび方法

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