HUE029181T2 - Herbicid-rezisztens napraforgó növények, herbicid-rezisztens acetohidroxisav szintetáz nagy alegység fehérjét kódoló polinukleotidok, és alkalmazási eljárásaik - Google Patents

Herbicid-rezisztens napraforgó növények, herbicid-rezisztens acetohidroxisav szintetáz nagy alegység fehérjét kódoló polinukleotidok, és alkalmazási eljárásaik Download PDF

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HUE029181T2
HUE029181T2 HUE12183100A HUE12183100A HUE029181T2 HU E029181 T2 HUE029181 T2 HU E029181T2 HU E12183100 A HUE12183100 A HU E12183100A HU E12183100 A HUE12183100 A HU E12183100A HU E029181 T2 HUE029181 T2 HU E029181T2
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herbicide
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ala
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Carlos Alberto Sala
Adriana Mariel Echarte
Mariano Bulos
Sherry R Whitt
Robert Ascenzi
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Basf Se
Nidera Sa
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Claims (8)

  1. napraforgó növények, hesEfeicid-fe^Äns ac®tohí<feöxísav szinieiáz nagy alegység: fehérjét kódoló poiintttóeöíidok, és alkalmazik eljárásaik SZABADALMI IOÉNYPONTÖK IV Napraforgó aeetohidroxisav sxÉtóétá&amp; nagy alegység faeelohydroxyaeld spfhâse large sAuráí, AîîASL) pofeÄotid, akel m említett ÂHÀSL .poiinukleotid egy iöMdazólfooá heihicrÚdolefáns AHASLl fehérjét kódol, amely a t. ssátM szekvencia 7-es ámmoáav ftózie tóban, vagy ekvivalens módon a 12, szántó szekvencia fí)?-es posádójáte- egy iúsmmáí íxmiúmá.· történő szutxsztitddót tartalmaz, ahol az említed Ál-IASL.t lekég© egy olyan poMpeptktó· kódol, amely legalább 95 9kban azonos &amp; 12. szántó szekvenciával* és ah»! u említett AílASLl fehérje egy növénynek megnövelt toleranciát biztosít egy imldazolinon herbielddef zárnám a növény vad-típosó variánsához képest. 2. M 1. igénypont, szerinti AHASLl fehérje, ahol az említett AHASLl fehérje -az alábbiakat tartalmazza: a 2. sz, szekvencia szerinti aminosav-szekveooia, -áltól a 2. sz. szekvencia 7-es pozíciójában, vagy ekvivalens ntódon a 12. szántó szekvencia 107-es pozíciójában egy alanínról treoníma történő szubsztitúció található, és amely adott eseten legalább egy, m alábbi csoportból választott elemmel mndeikezik; (I) egy prol inról gltóannora vagy szemre történő szebsztrtóeiö a 182~es pozícióban vagy a 12. szántó szekvenciában található amirmss v-szek véne iához viszonyít»« ekvivalens pozícióban; f ii) egy dmmä aszpartátm vagy valtóta történő szebsztitetó a J 90-es pozícióban vagy a 12. számtl szekvenciában található antótossv-szekveneiához viszonyított, ekvivalens ftózíeiöbam (iii> egy tríptoíánréi feocinra történő szubsztitúció az 559-es pozícrőbmr vagy a 12, szántó szekvenciában tóiSL ható Ímánosav-szekvenciálioz viszonyítod ekvivalens pozíckibao; és; (iv) alanínról aszparaginra, ímmósme feniklatónra vagy va.ii.nm történő szuOvuíucrő a Ó38-es .pozícióban vagy a 12.: szántó szekvenciában található aminosav-szekvenciákoz viszmyksÉtekvivalens pozícióban.
  2. 3. Expresszrós tórxeita, amely egy az ï. vagy 2. igénypont szerinti polinukleotidhoz működőképesen kapcsolt promoted, tartalmaz.
  3. 4. EffMs egy imidazoisnoo tebtdddel szemben toleráns: növény előállítására, amely eljátós m alábbiakat tartalmazza: fi). a növényi sejtet egy olyan poUnukleoíki konstrokttat tmnszíbrmágak, amely az L vagy 2, igénypont szerinti poltmMeoíiáot tartalmma mt&amp;ödoképesen kapcsolva egy promoterhez, amely promoter egy növényi sejtben sa expressziét vezeti; és fis) az említeti íranszíonaált sejtből regeneráljuk a növényt.
  4. 5. Növény, amely legalább egy képiát tartalmaz az I. vagy 2. igénypont szerinti pöfimikteotídbé!,
  5. 6. EijMs egy .ömszfomÄ növényi sejt kiválasztására, amely eljárás az alábbi lépéseket tartalmazza: t '0 egy növényi sejt biztosítása, .amely sejt egy növény ünnszíörmácios vétett tartalmaz, amely égy promoter! és egy az 1. vagy 2. igénypont szerinti, rnökodoképesen kapcsolt poünukleortdot tartalmaz; (ii) az említett transzformál í növényi sejtet imidazof herbicid hatásának tesszük ki, olyan feneentráeió melleit, amely gátolja egy nem tmrrtzíbrrnált növényi sejt növekedését; éé (Ili) az említett növényi sejtet azmmsájok a beállóid jeleÂiébeo matatod: fejlődési képessége afepján,
  6. 7. EijMs egy 5. igénypont szerinti növény termesztésén* az ernlített növény környezetében tévő gyomok irtása mellett, amely eljárás az alábbiakat tartalmazza: 0) a szabadban egy 5, igénypont szerinti oövényt tadesztönk; és (ii) az említett növényt egy AHAS-gátlo berbieiö Mszteény hatásos mennyiségével hozzuk kölcsönhatásba, amely készítmény egy Imidasolmort herbteklet, agy szulMlkarfeamid-herbícidet, egy trlazolopkinidirs-herbicidet, egy ptríniidiniioxi -benzoáí herbtddeí, egy szadfoottarntno-karboniltrsazolman herbkddet vagy ezek keverékét tartalmazza, amellyel szemben a növény toleráns, és amely megakadályozná a növény vad típusának növekedését, aboi a herbicid készítmény nem gátolja az említett növény növekedését olyan komoly mértékben, mint amennyire a növény vad típusának növekedését gátolná, 8, A 4., Ö, vagy 7, igénypontok bármelyike szerinti djMs. ahol az ernlíted hnidazohnon herbicid az alábbiak közül egyet -vagy többet tartalmaz; inntmlapir, hnazapte, Ishmox, Imazak®, imaxetabeoz és imazapir vagy az előbbiek bárnnlyea keveréke, 9, A 7, igénypont szerinti eljárás, ahol az említett szitlfönlíkarbamid herbicid kldmzuláaoot, metenlfomn metili, szulibmetmm metiliklörisnyron etilt, ti&amp;osznllomn metllí, tribennroa rnetik, bestsznlfuroo metili, nikoszulhiront, etnrnet szol túrón métáit, rimsznlíbronf, Irlíhrszulíozon snetiit, triaszulárront, ptisnisznlíorm médit, einosznlínrom, anúdosznlíhroni, iamxzulíuront, imazosznlínront, pímzosznlferon etilt, haloszuffmont vagy az előbbiek bártnllyesr keverékét tatfelimm.
  7. 10, Az S. igénypont szerinti növény bármely szerve, szövete vagy sejtje.
    1. Mag, amely az !. vagy 2. igénypont szerimi pol Inukleotkl közti fegalibb egyet «stai 12, A 11, igénypont szerinti mag, ahol az ernlfte« slagot egy AHAS-gátié herbieid hatásos meny-nyiségével kezeljük, 13. A 12, igénypont szerinti mag, adói az említett AHAS-gáíié berbieid egy nnidazobnon herblci-det, .egy szeifonilkarbamid herbieidet, egy triazolopirímidt» herbicides, egy szuil^dlamino--fearfeonMtriazeltooa herbicides vagy ezek bármilyen keverékéi -arsslmazza. 14, A 13, igénypont szerinti tnag, ahol az AMAS-gátló herhlcld egy irnidazolmon herhlcld.
  8. 15. Eljárás magok kezelésére, amely eljárás során a 51-14. igénypontok bármelyike szerinti magot egy AHAS-gátlö berbieid hatásos merrhyiségével kezeljük. Ki, A 15. igénypont szerinti eljárás, ahol ü etBlített AliAS-gátlo herbielcl egy inrídszoimon herbi-eidet, egy szalfenilkarharnld herbleidet, egy triazoloptrimldin herbicides, egy sznlfeiilamlno·-karbomlsriazolmon herbieidet vagy ezek bármilyen keverek«!: tartalmazza, 17. A 16. igénypont szerinti eljárás, ahol az említett AlMS-gátM herbldd egy irnidazolmon herbi-eld. 1 g. Eljárás napraforgó .4ÍM5L1 detektálására., amely e§árás az alábbiakat tartalmazza: (i) kinyerjük egy növény genomikus DMS-ét; («) az említeti DNS-t mintaként alkalmazzak egy poíimerlz lánemakeié (PCR) amplirikácséhan, amely egy a 7. ss, szekvenciát tartalmaző forward AÎÎâSU prlrrsert és egy a S. sz, szekvenciát hotalmaző reverz ÁííÁSLI prb men tartalmaz; és (ni) szekvenáljnk a PG.R amplirikácié sósán kapott termékeket.
HUE12183100A 2005-07-01 2006-06-29 Herbicid-rezisztens napraforgó növények, herbicid-rezisztens acetohidroxisav szintetáz nagy alegység fehérjét kódoló polinukleotidok, és alkalmazási eljárásaik HUE029181T2 (hu)

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MX2007010552A (es) * 2005-03-02 2009-02-19 Inst Nac De Tecnologia Agropec Plantas de arroz resistentes a herbicidas, polinucleotidos que codifican proteinas de subunidad grande de acido acetohidroxi sintasa resistentes a herbicidas, y sus metodos de uso.
EA020462B1 (ru) * 2005-07-01 2014-11-28 Басф Се Резистентные к гербицидам растения подсолнечника, полинуклеотиды, кодирующие резистентные к гербицидам большие субъединицы белков ацетогидроксикислотной синтазы, и применение растений и полинуклеотидов
AR064180A1 (es) * 2006-12-07 2009-03-18 Univ Kansas State Sorgo resistente a herbicida de acetolactato sintasa
UA108733C2 (uk) 2006-12-12 2015-06-10 Толерантна до гербіциду рослина соняшника
US10017827B2 (en) 2007-04-04 2018-07-10 Nidera S.A. Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use
AU2013270632A1 (en) * 2007-04-04 2014-01-16 Basf Plant Science Gmbh AHAS mutants
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