HRP20210092T1 - Pripravak medija za pripremu botulinum toksina - Google Patents
Pripravak medija za pripremu botulinum toksina Download PDFInfo
- Publication number
- HRP20210092T1 HRP20210092T1 HRP20210092TT HRP20210092T HRP20210092T1 HR P20210092 T1 HRP20210092 T1 HR P20210092T1 HR P20210092T T HRP20210092T T HR P20210092TT HR P20210092 T HRP20210092 T HR P20210092T HR P20210092 T1 HRP20210092 T1 HR P20210092T1
- Authority
- HR
- Croatia
- Prior art keywords
- botulinum toxin
- preparation
- medium
- hydrolyzate
- medium composition
- Prior art date
Links
- 108030001720 Bontoxilysin Proteins 0.000 title claims 5
- 229940053031 botulinum toxin Drugs 0.000 title claims 5
- 239000013028 medium composition Substances 0.000 title claims 2
- 238000011177 media preparation Methods 0.000 claims 4
- 239000001888 Peptone Substances 0.000 claims 3
- 108010080698 Peptones Proteins 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 235000019319 peptone Nutrition 0.000 claims 3
- 241000193155 Clostridium botulinum Species 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 claims 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims 2
- 235000019797 dipotassium phosphate Nutrition 0.000 claims 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims 2
- 235000019800 disodium phosphate Nutrition 0.000 claims 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000011707 mineral Substances 0.000 claims 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims 2
- 229940066779 peptones Drugs 0.000 claims 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 2
- 238000002360 preparation method Methods 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229920000742 Cotton Polymers 0.000 claims 1
- 108010068370 Glutens Proteins 0.000 claims 1
- 239000007836 KH2PO4 Substances 0.000 claims 1
- 240000004713 Pisum sativum Species 0.000 claims 1
- 235000016816 Pisum sativum subsp sativum Nutrition 0.000 claims 1
- 241000209140 Triticum Species 0.000 claims 1
- 235000021307 Triticum Nutrition 0.000 claims 1
- 235000021120 animal protein Nutrition 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 235000021312 gluten Nutrition 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 239000001488 sodium phosphate Substances 0.000 claims 1
- 235000013311 vegetables Nutrition 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Claims (7)
1. Pripravak medija životinjskog proteina (APF) za uporabu u kulturi Clostridium botulinum, pripravak medija sadrži:
peptone biljnog porijekla koji sadrže hidrolizat vrtnog graška, hidrolizat sjemena pamuka i hidrolizat pšeničnog glutena u omjeru 1: 0.24-43.62: 0.01-50.57 težinskih; izvor ugljika; i najmanje jedan mineral odabran iz skupine koja se sastoji od K2HPO4 (dikalijev fosfat), Na2HPO4 (dinatrijev fosfat) i KH2PO4 (monokalijev fosfat).
2. Pripravak medija prema patentnom zahtjevu 1, naznačen time što su peptoni biljnog porijekla sadržani od 0.1 -10 w / v%.
3. Pripravak medija prema zahtjevu 1, naznačen time što je pepton biljnog porijekla podvrgnut enzimskom tretmanu.
4. Pripravak medija prema zahtjevu 1, naznačen time što se mineral nalazi u pripravku medija sa sadržajem 0.05-3.5 w/v%.
5. Postupak za proizvodnju botulinum toksina, koji obuhvaća korake:
(a) uzgoj Clostridium botulinum uporabom pripravka medija prema bilo kojem od zahtjeva 1 do 4 za proizvodnju botulinum toksina; i
(b) obnavljanje proizvedenog botulinum toksina.
6. Postupak prema zahtjevu 5, naznačen time što se uzgoj vrši u anaerobnim uvjetima.
7. Postupak prema zahtjevu 5, naznačen time što je botulinum toksin odabran iz skupine koja se sastoji od serotipova A, B, C, D, E, F i G.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150059654A KR101723167B1 (ko) | 2015-04-28 | 2015-04-28 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| EP16786751.4A EP3289069B1 (en) | 2015-04-28 | 2016-04-28 | Medium composition for preparing botulinum toxin |
| PCT/KR2016/004430 WO2016175565A1 (en) | 2015-04-28 | 2016-04-28 | Medium composition for preparing botulinum toxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20210092T1 true HRP20210092T1 (hr) | 2021-04-30 |
Family
ID=57199460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20210092TT HRP20210092T1 (hr) | 2015-04-28 | 2021-01-19 | Pripravak medija za pripremu botulinum toksina |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US9926549B2 (hr) |
| EP (1) | EP3289069B1 (hr) |
| JP (2) | JP2018500880A (hr) |
| KR (1) | KR101723167B1 (hr) |
| CN (1) | CN107109354A (hr) |
| AR (1) | AR104419A1 (hr) |
| AU (1) | AU2016253788B2 (hr) |
| BR (1) | BR112017012885A2 (hr) |
| CA (1) | CA2968041A1 (hr) |
| DK (1) | DK3289069T3 (hr) |
| ES (1) | ES2846927T3 (hr) |
| HR (1) | HRP20210092T1 (hr) |
| HU (1) | HUE052935T2 (hr) |
| MX (1) | MX2017008197A (hr) |
| PL (1) | PL3289069T3 (hr) |
| PT (1) | PT3289069T (hr) |
| RU (1) | RU2663586C1 (hr) |
| SA (1) | SA517381720B1 (hr) |
| TW (1) | TWI592484B (hr) |
| WO (1) | WO2016175565A1 (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101723167B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101723168B1 (ko) * | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR102209159B1 (ko) * | 2019-03-29 | 2021-01-29 | (주)제테마 | 독소의 제조방법 |
| GEP20247609B (en) * | 2019-12-20 | 2024-03-11 | Ipsen Biopharm Ltd | Ethod of producing botulinum toxin |
| KR102581772B1 (ko) * | 2021-03-30 | 2023-09-22 | 강원대학교 산학협력단 | 식물 및 곤충 단백질 가수 분해물을 이용한 배양육 제조를 위한 최적 배양 방법 및 그 응용 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5409799A (en) * | 1998-07-03 | 2000-01-24 | Dsm N.V. | Fermentation process to produce clavulanic acid at a low concentration of free amino acids |
| US6558926B1 (en) | 1999-07-16 | 2003-05-06 | Massachusetts Institute Of Technology | Method for production of tetanus toxin using media substantially free of animal products |
| US7160699B2 (en) | 2003-09-25 | 2007-01-09 | Allergan, Inc. | Media for clostridium bacterium and processes for obtaining a clostridial toxin |
| US7148041B2 (en) * | 2003-09-25 | 2006-12-12 | Allergan, Inc. | Animal product free media and processes for obtaining a botulinum toxin |
| RU2255761C1 (ru) * | 2004-07-01 | 2005-07-10 | Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" | Препарат для лечения мышечных дистоний из токсина культуры clostridium botulinum и способ его получения |
| WO2006042542A2 (en) | 2004-10-19 | 2006-04-27 | Statens Serum Institut | Production of tetanus, diphtheria, and pertussis toxins and toxoids using fermentation media containing no components of animal or soy origin |
| CA2556796C (en) * | 2005-03-03 | 2018-01-23 | Allergan, Inc. | Animal product free system and process for purifying a botulinum toxin |
| NL1029059C2 (nl) | 2005-05-17 | 2006-11-20 | Noord Nl Oliemolen Holding B V | Peptidenpreparaat voor het groeien en/of kweken van micro-organismen en/of cellen. |
| FR2918671B1 (fr) * | 2007-07-10 | 2010-10-15 | Sanofi Pasteur | Milieu de culture d'haemophilus influenzae type b. |
| CN101698826A (zh) * | 2007-12-19 | 2010-04-28 | 杨宇 | 胰酪胨肉毒梭菌增菌肉汤 |
| KR20090120222A (ko) | 2008-05-19 | 2009-11-24 | (주)메디톡스 | 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법 |
| US8236356B2 (en) * | 2008-06-11 | 2012-08-07 | Roche Diagnostics Operations, Inc. | Growth medium for Clostridium histolyticum |
| BR102012013110A2 (pt) | 2012-05-31 | 2014-05-27 | Cristalia Prod Quimicos Farm | Meio de cultura para bactérias do gênero clostridium livre de componentes de origem animal e processo para produção de sobrenadante contendo uma ou mais proteases com atividade colagenolítica e gelatinolítica |
| BR122016023101B1 (pt) | 2012-10-21 | 2022-03-22 | Pfizer Inc | Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile |
| CN103215310B (zh) * | 2013-03-27 | 2015-01-21 | 中棉紫光生物科技（上海）有限公司 | 用于发酵的棉籽蛋白及其制备方法和应用 |
| KR101339349B1 (ko) | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| KR101723167B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101723168B1 (ko) * | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101729251B1 (ko) * | 2015-04-28 | 2017-04-21 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
-
2015
- 2015-04-28 KR KR1020150059654A patent/KR101723167B1/ko active IP Right Grant
-
2016
- 2016-04-27 TW TW105113079A patent/TWI592484B/zh active
- 2016-04-27 AR ARP160101185A patent/AR104419A1/es unknown
- 2016-04-28 CN CN201680004195.4A patent/CN107109354A/zh active Pending
- 2016-04-28 HU HUE16786751A patent/HUE052935T2/hu unknown
- 2016-04-28 RU RU2017120811A patent/RU2663586C1/ru active
- 2016-04-28 DK DK16786751.4T patent/DK3289069T3/da active
- 2016-04-28 PL PL16786751T patent/PL3289069T3/pl unknown
- 2016-04-28 BR BR112017012885A patent/BR112017012885A2/pt active Search and Examination
- 2016-04-28 AU AU2016253788A patent/AU2016253788B2/en active Active
- 2016-04-28 ES ES16786751T patent/ES2846927T3/es active Active
- 2016-04-28 US US15/521,817 patent/US9926549B2/en active Active
- 2016-04-28 EP EP16786751.4A patent/EP3289069B1/en active Active
- 2016-04-28 JP JP2017524476A patent/JP2018500880A/ja active Pending
- 2016-04-28 PT PT167867514T patent/PT3289069T/pt unknown
- 2016-04-28 WO PCT/KR2016/004430 patent/WO2016175565A1/en active Application Filing
- 2016-04-28 MX MX2017008197A patent/MX2017008197A/es unknown
- 2016-04-28 CA CA2968041A patent/CA2968041A1/en not_active Abandoned
-
2017
- 2017-06-14 SA SA517381720A patent/SA517381720B1/ar unknown
-
2018
- 2018-02-09 US US15/893,297 patent/US10465179B2/en active Active
-
2019
- 2019-06-05 JP JP2019105170A patent/JP6888049B2/ja active Active
-
2021
- 2021-01-19 HR HRP20210092TT patent/HRP20210092T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2016253788B2 (en) | 2018-12-13 |
| CN107109354A (zh) | 2017-08-29 |
| BR112017012885A2 (pt) | 2018-01-30 |
| DK3289069T3 (da) | 2021-01-25 |
| AR104419A1 (es) | 2017-07-19 |
| US10465179B2 (en) | 2019-11-05 |
| PT3289069T (pt) | 2021-02-11 |
| KR20160127997A (ko) | 2016-11-07 |
| PL3289069T3 (pl) | 2021-07-05 |
| MX2017008197A (es) | 2017-09-13 |
| US20170247675A1 (en) | 2017-08-31 |
| KR101723167B1 (ko) | 2017-04-05 |
| EP3289069A4 (en) | 2018-10-03 |
| HUE052935T2 (hu) | 2021-05-28 |
| WO2016175565A1 (en) | 2016-11-03 |
| RU2663586C1 (ru) | 2018-08-07 |
| US20180163193A1 (en) | 2018-06-14 |
| US9926549B2 (en) | 2018-03-27 |
| TW201638328A (zh) | 2016-11-01 |
| SA517381720B1 (ar) | 2021-03-07 |
| AU2016253788A1 (en) | 2017-05-25 |
| JP6888049B2 (ja) | 2021-06-16 |
| EP3289069A1 (en) | 2018-03-07 |
| EP3289069B1 (en) | 2020-12-09 |
| ES2846927T3 (es) | 2021-07-30 |
| JP2018500880A (ja) | 2018-01-18 |
| TWI592484B (zh) | 2017-07-21 |
| CA2968041A1 (en) | 2016-11-03 |
| JP2020022435A (ja) | 2020-02-13 |
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