HK1210476A1 - A method of weak partitioning chromatography - Google Patents

A method of weak partitioning chromatography

Info

Publication number
HK1210476A1
HK1210476A1 HK15111155.1A HK15111155A HK1210476A1 HK 1210476 A1 HK1210476 A1 HK 1210476A1 HK 15111155 A HK15111155 A HK 15111155A HK 1210476 A1 HK1210476 A1 HK 1210476A1
Authority
HK
Hong Kong
Prior art keywords
weak partitioning
partitioning chromatography
chromatography
weak
partitioning
Prior art date
Application number
HK15111155.1A
Other languages
English (en)
Chinese (zh)
Inventor
Paul Brown
Jon Coffman
Ranganathan Godavarti
Tim Iskra
Brian D Kelley
Suresh Vunnum
Shujun Sun
Tianning Yu
James Edward Booth
Mary Beth Switzer
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36992346&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK1210476(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of HK1210476A1 publication Critical patent/HK1210476A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
HK15111155.1A 2005-03-11 2015-11-12 A method of weak partitioning chromatography HK1210476A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US66043705P 2005-03-11 2005-03-11

Publications (1)

Publication Number Publication Date
HK1210476A1 true HK1210476A1 (en) 2016-04-22

Family

ID=36992346

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15111155.1A HK1210476A1 (en) 2005-03-11 2015-11-12 A method of weak partitioning chromatography

Country Status (21)

Country Link
US (3) US8067182B2 (ru)
EP (1) EP1869065B1 (ru)
JP (1) JP5199063B2 (ru)
KR (2) KR101482791B1 (ru)
CN (2) CN104610422A (ru)
AU (1) AU2006223180B2 (ru)
BR (1) BRPI0608584B8 (ru)
CA (1) CA2601062C (ru)
CR (1) CR9377A (ru)
DK (1) DK1869065T3 (ru)
ES (1) ES2797480T3 (ru)
HK (1) HK1210476A1 (ru)
HU (1) HUE049797T2 (ru)
IL (1) IL185687A (ru)
MX (1) MX2007011129A (ru)
NO (1) NO20074443L (ru)
PL (1) PL1869065T3 (ru)
PT (1) PT1869065T (ru)
RU (1) RU2457214C2 (ru)
SI (1) SI1869065T1 (ru)
WO (1) WO2006099308A2 (ru)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104610422A (zh) 2005-03-11 2015-05-13 惠氏公司 弱分配层析的方法
JP2010510963A (ja) * 2006-06-14 2010-04-08 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー セラミックヒドロキシアパタイトを使用する抗体の精製方法
ES2755386T5 (es) 2006-08-28 2023-04-05 Ares Trading Sa Proceso para la purificación de proteínas que contienen fragmentos Fc
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
WO2008033517A2 (en) 2006-09-13 2008-03-20 Abbott Laboratories Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
PL2078071T3 (pl) * 2006-11-08 2015-07-31 Wyeth Llc Racjonalnie opracowane pożywki do hodowli komórek
PL2840090T3 (pl) * 2007-10-30 2018-07-31 Genentech, Inc. Oczyszczanie przeciwciał za pomocą chromatografii kationowymiennej
EP2238154B1 (en) 2008-01-18 2015-09-16 Bio-Rad Laboratories, Inc. Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography
SI2848625T1 (sl) 2008-08-14 2019-11-29 Genentech Inc Postopki za odstranjevanje kontaminanta z uporabo ionskoizmenjevalne membranske kromatografije z izpodrinjenjem nativnega proteina
WO2010030222A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal with multimodal anion exchangers in the presence of protein-excluded zwitterions
US20100069617A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions
EP2921501A1 (en) * 2008-10-20 2015-09-23 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
EP3011953A1 (en) 2008-10-29 2016-04-27 Ablynx N.V. Stabilised formulations of single domain antigen binding molecules
WO2010056550A1 (en) 2008-10-29 2010-05-20 Wyeth Llc Methods for purification of single domain antigen binding molecules
BRPI0923541A2 (pt) * 2008-12-22 2016-01-26 Hoffmann La Roche purificação de imunoglobulina
WO2011073389A1 (en) * 2009-12-18 2011-06-23 Novartis Ag Wash solution and method for affinity chromatography
CA2787009C (en) 2010-01-15 2018-04-03 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
SG10201710439UA (en) * 2010-05-25 2018-01-30 Genentech Inc Methods of purifying polypeptides
EP2583973B1 (en) * 2010-06-21 2018-03-21 Kyowa Hakko Kirin Co., Ltd. Method for purifying protein using amino acid
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
WO2012014183A1 (en) 2010-07-30 2012-02-02 Pfizer Inc. Tandem purification of proteins
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
US20130331554A1 (en) 2010-11-15 2013-12-12 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
US9592540B2 (en) 2011-02-02 2017-03-14 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US10221210B2 (en) 2011-07-20 2019-03-05 Zepteon, Incorporated Polypeptide separation methods
ES2717536T3 (es) 2011-11-02 2019-06-21 Hoffmann La Roche Cromatografía de sobrecarga y elución
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) * 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
CN104507952B (zh) * 2012-05-30 2018-08-10 生物辐射实验室股份有限公司 基于磷灰石的色谱树脂的原位恢复
EP2855504B1 (en) * 2012-05-31 2018-10-24 Agency For Science, Technology And Research Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014035475A1 (en) 2012-09-02 2014-03-06 Abbvie Inc. Methods to control protein heterogeneity
EP2931400B1 (en) 2012-12-14 2021-07-07 Cytiva BioProcess R&D AB Method for cleaning of packed bed chromatography columns
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
WO2014159441A1 (en) * 2013-03-14 2014-10-02 Amgen Inc. Removal of leaked affinity purification ligand
TWI670279B (zh) 2013-03-15 2019-09-01 美商艾爾德生物製藥股份有限公司 抗體純化及純度監測
AR096713A1 (es) * 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
RU2016107435A (ru) 2013-09-13 2017-10-18 Дженентек, Инк. Композиции и способы обнаружения и количественного определения белка клеток-хозяев в клеточных линиях и рекомбинантные полипептидные продукты
SG11201601823TA (en) 2013-09-13 2016-04-28 Genentech Inc Methods and compositions comprising purified recombinant polypeptides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
EP3157689B1 (en) 2014-06-23 2021-04-28 Bio-Rad Laboratories, Inc. Apatite pretreatment
EP3157551B1 (en) 2014-06-23 2023-02-15 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
EP3487868A4 (en) 2016-07-25 2020-07-29 Cephalon, Inc. WASHING PAD FOR AFFINITY CHROMATOGRAPHY
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
JP6951051B2 (ja) * 2018-05-24 2021-10-20 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法
US20220267369A1 (en) * 2018-07-25 2022-08-25 Merck Sharp & Dohme Corp. Methods of separating host cell lipases from a production protein in chromatographic processes
WO2020172658A1 (en) 2019-02-24 2020-08-27 Bristol-Myers Squibb Company Methods of isolating a protein
WO2020191369A1 (en) 2019-03-21 2020-09-24 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
WO2021154908A1 (en) * 2020-01-29 2021-08-05 Merck Sharp & Dohme Corp. Methods of separating host cell lipases from an anti-lag3 antibody production
US20230366794A1 (en) 2020-09-23 2023-11-16 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
WO2022066928A2 (en) 2020-09-23 2022-03-31 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
IL310556A (en) 2021-08-23 2024-03-01 Genentech Inc Purification processes using cation exchange chromatography for drug-antibody conjugates

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4029583A (en) * 1975-02-28 1977-06-14 Purdue Research Foundation Chromatographic supports and methods and apparatus for preparing the same
JPS63126826A (ja) * 1986-11-14 1988-05-30 Asahi Chem Ind Co Ltd プラスミノ−ゲン活性化因子誘導物質
US5451662A (en) * 1987-10-23 1995-09-19 Schering Corporation Method of purifying protein
HU204537B (en) * 1987-10-23 1992-01-28 Schering Corp Process for purifying gm-csf
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
ATE114668T1 (de) 1988-10-26 1994-12-15 American Cyanamid Co Verfahren zur verringerung des aggregatinhaltes in wachstumshormonen gleichenden stoffen.
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5264555A (en) 1992-07-14 1993-11-23 Enzon, Inc. Process for hemoglobin extraction and purification
FR2703049B1 (fr) * 1993-03-22 1995-04-21 Rhone Poulenc Rorer Sa Procédé de purification des taxoïdes.
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
AU7241294A (en) * 1994-04-01 1995-10-23 Unisyn Technologies, Inc. High salt binding buffer for immunoglobulin purification with a synthetic affinity ligand
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
RU2094077C1 (ru) * 1996-07-23 1997-10-27 Акционерное общество закрытого типа "Фосфосорб" Хроматографический способ выделения альфа-фетопротеина
TW505655B (en) * 1997-10-14 2002-10-11 Tanox Inc Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography
US6504013B1 (en) 2000-02-01 2003-01-07 Idexx Laboratories, Inc. Canine allergy therapeutic recombinant chimeric anti-IgE monoclonal antibody
WO2000015260A1 (en) 1998-09-16 2000-03-23 Tanox, Inc. Anti-ige gene therapy
ATE266672T1 (de) * 1998-12-30 2004-05-15 Folke Tjerneld Trennungsmethode unter verwendung von flüssig- flüssig partition
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
AU2004215653B2 (en) * 2003-02-28 2011-03-17 Lonza Biologics Plc. Antibody purification by protein A and ion exchange chromatography
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
JP4496168B2 (ja) * 2003-07-28 2010-07-07 株式会社リバース・プロテオミクス研究所 非特異的物質の除去方法
ATE462716T1 (de) 2003-10-24 2010-04-15 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
CN1898266B (zh) 2003-10-27 2011-09-21 惠氏公司 使用羟磷灰石层析除去高分子量聚集体
CN104610422A (zh) 2005-03-11 2015-05-13 惠氏公司 弱分配层析的方法

Also Published As

Publication number Publication date
JP5199063B2 (ja) 2013-05-15
CA2601062C (en) 2016-08-02
US20070060741A1 (en) 2007-03-15
KR20130128020A (ko) 2013-11-25
EP1869065A4 (en) 2012-07-04
ES2797480T3 (es) 2020-12-02
AU2006223180B2 (en) 2011-11-24
CN101175765A (zh) 2008-05-07
US8067182B2 (en) 2011-11-29
RU2007132851A (ru) 2009-04-20
NO20074443L (no) 2007-09-27
US20070054390A1 (en) 2007-03-08
WO2006099308A2 (en) 2006-09-21
SI1869065T1 (sl) 2020-08-31
MX2007011129A (es) 2007-11-06
JP2008533473A (ja) 2008-08-21
KR20070121667A (ko) 2007-12-27
BRPI0608584B1 (pt) 2018-02-06
IL185687A0 (en) 2008-01-06
EP1869065A2 (en) 2007-12-26
CN104610422A (zh) 2015-05-13
DK1869065T3 (da) 2020-06-08
KR101482791B1 (ko) 2015-01-21
RU2457214C2 (ru) 2012-07-27
IL185687A (en) 2015-05-31
CA2601062A1 (en) 2006-09-21
CR9377A (es) 2008-02-21
WO2006099308A3 (en) 2007-10-25
US9144755B2 (en) 2015-09-29
KR101660575B1 (ko) 2016-09-27
EP1869065B1 (en) 2020-05-06
US20120138537A1 (en) 2012-06-07
PT1869065T (pt) 2020-06-18
HUE049797T2 (hu) 2020-10-28
PL1869065T3 (pl) 2020-09-21
AU2006223180A1 (en) 2006-09-21
BRPI0608584B8 (pt) 2021-05-25
BRPI0608584A2 (pt) 2010-01-19

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