GB720232A - Preparation of catalase - Google Patents

Preparation of catalase

Info

Publication number
GB720232A
GB720232A GB1995052A GB1995052A GB720232A GB 720232 A GB720232 A GB 720232A GB 1995052 A GB1995052 A GB 1995052A GB 1995052 A GB1995052 A GB 1995052A GB 720232 A GB720232 A GB 720232A
Authority
GB
United Kingdom
Prior art keywords
water
precipitate
bring
separated
alum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1995052A
Inventor
Havard Lawrence Keil
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Armour and Co
Original Assignee
Armour and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Armour and Co filed Critical Armour and Co
Priority to GB1995052A priority Critical patent/GB720232A/en
Publication of GB720232A publication Critical patent/GB720232A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

Abstract

Whole blood or separated blood cells are diluted with water and the pH brought to 5.5-4.0 in the presence of an alum to precipitate a catalase complex. The latter may be separated and dried, or mixed with glycerol to give a stable product, or further purified by dispersing in water, bringing the pH to 7-8 (e.g. with sodium citrate), acidifying to pH 5.5-4, precipitating with ethanol and drying the precipitate. Preferably the temperature is kept around 5 DEG C. throughout. The initial fall in pH may be effected with the alum solution alone, or an acid such as sulphuric first added to bring the pH down to 6.0. Preferred pH values for the respective steps are 4.8 and 7.2. In a modification the initial water is treated with carbon dioxide (preferably in solid form) to bring its pH to 5.5-6.0. Examples are given of the process, using sodium aluminium sulphate as precipitant; chrome and ferric alums are also stated to be suitable. Specification 713,720 is referred to.
GB1995052A 1952-08-07 1952-08-07 Preparation of catalase Expired GB720232A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1995052A GB720232A (en) 1952-08-07 1952-08-07 Preparation of catalase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1995052A GB720232A (en) 1952-08-07 1952-08-07 Preparation of catalase

Publications (1)

Publication Number Publication Date
GB720232A true GB720232A (en) 1954-12-15

Family

ID=10137834

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1995052A Expired GB720232A (en) 1952-08-07 1952-08-07 Preparation of catalase

Country Status (1)

Country Link
GB (1) GB720232A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4341867A (en) 1980-04-21 1982-07-27 De Forende Bryggerier A/S Process for recovering enzymes from blood

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4341867A (en) 1980-04-21 1982-07-27 De Forende Bryggerier A/S Process for recovering enzymes from blood

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