GB2378245A - Nucleic acid amplification method - Google Patents
Nucleic acid amplification method Download PDFInfo
- Publication number
- GB2378245A GB2378245A GB0118959A GB0118959A GB2378245A GB 2378245 A GB2378245 A GB 2378245A GB 0118959 A GB0118959 A GB 0118959A GB 0118959 A GB0118959 A GB 0118959A GB 2378245 A GB2378245 A GB 2378245A
- Authority
- GB
- United Kingdom
- Prior art keywords
- probe
- probes
- amplification
- product
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 106
- 239000000523 sample Substances 0.000 claims abstract description 165
- 230000003321 amplification Effects 0.000 claims abstract description 103
- 238000000034 method Methods 0.000 claims abstract description 86
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 40
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 34
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 34
- 239000000178 monomer Substances 0.000 claims abstract description 19
- 238000005096 rolling process Methods 0.000 claims abstract description 6
- 239000000047 product Substances 0.000 claims description 128
- 238000006243 chemical reaction Methods 0.000 claims description 38
- 108091034117 Oligonucleotide Proteins 0.000 claims description 37
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 30
- 230000000295 complement effect Effects 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 230000015572 biosynthetic process Effects 0.000 claims description 17
- 238000009396 hybridization Methods 0.000 claims description 17
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- 108091008146 restriction endonucleases Proteins 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 13
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- 238000010791 quenching Methods 0.000 claims description 11
- 230000000171 quenching effect Effects 0.000 claims description 11
- 238000010494 dissociation reaction Methods 0.000 claims description 7
- 230000005593 dissociations Effects 0.000 claims description 7
- 108010042407 Endonucleases Proteins 0.000 claims description 6
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 6
- 239000003446 ligand Substances 0.000 claims description 6
- 230000010076 replication Effects 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 238000009877 rendering Methods 0.000 claims description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 4
- 108060002716 Exonuclease Proteins 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 102000013165 exonuclease Human genes 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000007795 chemical reaction product Substances 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 239000002299 complementary DNA Substances 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000005382 thermal cycling Methods 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 230000008684 selective degradation Effects 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 16
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 16
- 238000003776 cleavage reaction Methods 0.000 description 15
- 230000007017 scission Effects 0.000 description 15
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- 239000000872 buffer Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 8
- 238000012544 monitoring process Methods 0.000 description 8
- 238000013461 design Methods 0.000 description 7
- 102000012410 DNA Ligases Human genes 0.000 description 6
- 108010061982 DNA Ligases Proteins 0.000 description 6
- 102000003960 Ligases Human genes 0.000 description 5
- 108090000364 Ligases Proteins 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 102100031780 Endonuclease Human genes 0.000 description 4
- 101710163270 Nuclease Proteins 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 102000018120 Recombinases Human genes 0.000 description 3
- 108010091086 Recombinases Proteins 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 230000001036 exonucleolytic effect Effects 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108091064358 Holliday junction Proteins 0.000 description 1
- 102000039011 Holliday junction Human genes 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108010020713 Tth polymerase Proteins 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0118959A GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
| EP02741612.2A EP1423532B1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| EP10003678A EP2224016A1 (en) | 2001-08-03 | 2002-07-12 | Method for generating a circularised nucleic acid |
| JP2003517293A JP2004536617A (ja) | 2001-08-03 | 2002-07-12 | 核酸増幅方法 |
| CA002455803A CA2455803A1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| PCT/SE2002/001378 WO2003012119A2 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| AU2002314693A AU2002314693A1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| EP10003586.4A EP2251439B1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| US10/483,900 US7320860B2 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| EP10003679.7A EP2236622B1 (en) | 2001-08-03 | 2002-07-12 | Method for generating circularised nucleic acid |
| US11/946,706 US7790388B2 (en) | 2001-08-03 | 2007-11-28 | Nucleic acid amplification method |
| JP2008316989A JP4559517B2 (ja) | 2001-08-03 | 2008-12-12 | 核酸増幅方法 |
| JP2009230088A JP4675424B2 (ja) | 2001-08-03 | 2009-10-02 | 核酸増幅方法 |
| JP2010125694A JP5313204B2 (ja) | 2001-08-03 | 2010-06-01 | 核酸増幅方法 |
| US13/329,998 USRE44265E1 (en) | 2001-08-03 | 2011-12-19 | Nucleic acid amplification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0118959A GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0118959D0 GB0118959D0 (en) | 2001-09-26 |
| GB2378245A true GB2378245A (en) | 2003-02-05 |
Family
ID=9919749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0118959A Withdrawn GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7320860B2 (enExample) |
| EP (4) | EP2251439B1 (enExample) |
| JP (4) | JP2004536617A (enExample) |
| AU (1) | AU2002314693A1 (enExample) |
| CA (1) | CA2455803A1 (enExample) |
| GB (1) | GB2378245A (enExample) |
| WO (1) | WO2003012119A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079042A1 (en) * | 2013-11-29 | 2015-06-04 | Q-Linea Ab | Rolling circle amplification method |
Families Citing this family (118)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| GB0304371D0 (en) * | 2003-02-26 | 2003-04-02 | Solexa Ltd | DNA Sequence analysis |
| US7385043B1 (en) * | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| WO2005113804A1 (en) * | 2004-05-20 | 2005-12-01 | Trillion Genomics Limited | Use of mass labelled probes to detect target nucleic acids using mass spectrometry |
| EP1784754A4 (en) * | 2004-08-13 | 2009-05-27 | Harvard College | OPTI-NANOPORE DNA READING PLATFORM WITH ULTRAHOLE THROUGHPUT |
| JP2008522628A (ja) * | 2004-12-11 | 2008-07-03 | サイトジェニックス， インコーポレイテッド | 高品質核酸のセルフリー生合成及びその使用 |
| US7601499B2 (en) | 2005-06-06 | 2009-10-13 | 454 Life Sciences Corporation | Paired end sequencing |
| JP5103398B2 (ja) * | 2005-06-06 | 2012-12-19 | ４５４ ライフ サイエンシーズ コーポレイション | 両末端配列決定（ｐａｉｒｅｄｅｎｄｓｅｑｕｅｎｃｉｎｇ） |
| US20090264299A1 (en) | 2006-02-24 | 2009-10-22 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| EP1907571B1 (en) | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
| US8460879B2 (en) | 2006-02-21 | 2013-06-11 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| US7910302B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090111706A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
| US7862999B2 (en) * | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
| US20100047792A1 (en) * | 2007-04-10 | 2010-02-25 | Szendroe Istvan | Label-free optical detection method |
| US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
| WO2009046445A1 (en) * | 2007-10-04 | 2009-04-09 | Halcyon Molecular | Sequencing nucleic acid polymers with electron microscopy |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| US8298768B2 (en) | 2007-11-29 | 2012-10-30 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| FR2924440B1 (fr) * | 2007-12-04 | 2015-01-09 | Pf Medicament | Nouveau procede de generation et de criblage d'une banque d'anticorps |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US20090233809A1 (en) * | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
| WO2010065568A2 (en) | 2008-12-01 | 2010-06-10 | Laboratory Corporation Of America Holdings | METHODS AND ASSAYS FOR MEASURING p95 AND/OR p95 IN A SAMPLE AND ANTIBODIES SPECIFIC FOR p95 |
| SG172983A1 (en) | 2009-01-15 | 2011-08-29 | Lab Corp America Holdings | Methods of determining patient response by measurement of her-3 |
| EP2387717B1 (en) * | 2009-01-15 | 2014-12-10 | Laboratory Corporation of America Holdings | Methods of determining patient response by measurement of her-2 expression |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| JP2012525147A (ja) | 2009-04-30 | 2012-10-22 | グッド スタート ジェネティクス， インコーポレイテッド | 遺伝マーカーを評価するための方法および組成物 |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
| WO2011066476A1 (en) * | 2009-11-25 | 2011-06-03 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| EP2542890B1 (en) | 2010-03-01 | 2015-05-06 | Quanterix Corporation | Methods for extending dynamic range in assays for the detection of molecules or particles |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| US9096952B2 (en) | 2010-06-24 | 2015-08-04 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
| US10669569B2 (en) | 2010-10-15 | 2020-06-02 | Navinci Diagnostics Ab | Dynamic range methods |
| CN101974638B (zh) * | 2010-11-10 | 2012-10-03 | 华东医学生物技术研究所 | 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法 |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| EP2673380B1 (en) | 2011-02-09 | 2018-12-12 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| US9556473B2 (en) | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| CA2827497C (en) | 2011-02-15 | 2014-12-02 | Leica Biosystems Newcastle Ltd. | Method for localized in situ detection of mrna |
| US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| US10597701B2 (en) | 2011-05-11 | 2020-03-24 | Navinci Diagnostics Ab | Unfolding proximity probes and methods for the use thereof |
| CA2852665A1 (en) | 2011-10-17 | 2013-04-25 | Good Start Genetics, Inc. | Analysis methods |
| WO2013128281A1 (en) | 2012-02-28 | 2013-09-06 | Population Genetics Technologies Ltd | Method for attaching a counter sequence to a nucleic acid sample |
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| EP1423532B1 (en) | 2013-05-22 |
| JP2009077735A (ja) | 2009-04-16 |
| WO2003012119A2 (en) | 2003-02-13 |
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| GB0118959D0 (en) | 2001-09-26 |
| US7790388B2 (en) | 2010-09-07 |
| EP1423532A2 (en) | 2004-06-02 |
| EP2236622B1 (en) | 2017-09-06 |
| JP2009297045A (ja) | 2009-12-24 |
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| US20080131899A1 (en) | 2008-06-05 |
| USRE44265E1 (en) | 2013-06-04 |
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| EP2236622A2 (en) | 2010-10-06 |
| US20050287526A1 (en) | 2005-12-29 |
| JP5313204B2 (ja) | 2013-10-09 |
| JP2004536617A (ja) | 2004-12-09 |
| EP2251439A1 (en) | 2010-11-17 |
| US7320860B2 (en) | 2008-01-22 |
| CA2455803A1 (en) | 2003-02-13 |
| EP2236622A3 (en) | 2010-11-03 |
| JP4559517B2 (ja) | 2010-10-06 |
| JP2010183916A (ja) | 2010-08-26 |
| EP2224016A1 (en) | 2010-09-01 |
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