GB2378245A - Nucleic acid amplification method - Google Patents
Nucleic acid amplification method Download PDFInfo
- Publication number
- GB2378245A GB2378245A GB0118959A GB0118959A GB2378245A GB 2378245 A GB2378245 A GB 2378245A GB 0118959 A GB0118959 A GB 0118959A GB 0118959 A GB0118959 A GB 0118959A GB 2378245 A GB2378245 A GB 2378245A
- Authority
- GB
- United Kingdom
- Prior art keywords
- probe
- probes
- amplification
- product
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 106
- 239000000523 sample Substances 0.000 claims abstract description 165
- 230000003321 amplification Effects 0.000 claims abstract description 103
- 238000000034 method Methods 0.000 claims abstract description 86
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 40
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 34
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 34
- 239000000178 monomer Substances 0.000 claims abstract description 19
- 238000005096 rolling process Methods 0.000 claims abstract description 6
- 239000000047 product Substances 0.000 claims description 128
- 238000006243 chemical reaction Methods 0.000 claims description 38
- 108091034117 Oligonucleotide Proteins 0.000 claims description 37
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 30
- 230000000295 complement effect Effects 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 230000015572 biosynthetic process Effects 0.000 claims description 17
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- 108091008146 restriction endonucleases Proteins 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 13
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- 238000010494 dissociation reaction Methods 0.000 claims description 7
- 230000005593 dissociations Effects 0.000 claims description 7
- 108010042407 Endonucleases Proteins 0.000 claims description 6
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 6
- 239000003446 ligand Substances 0.000 claims description 6
- 230000010076 replication Effects 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 238000009877 rendering Methods 0.000 claims description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 4
- 108060002716 Exonuclease Proteins 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 102000013165 exonuclease Human genes 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000007795 chemical reaction product Substances 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 239000002299 complementary DNA Substances 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000005382 thermal cycling Methods 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 230000008684 selective degradation Effects 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 16
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 16
- 238000003776 cleavage reaction Methods 0.000 description 15
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- 239000000872 buffer Substances 0.000 description 9
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- 238000012544 monitoring process Methods 0.000 description 8
- 238000013461 design Methods 0.000 description 7
- 102000012410 DNA Ligases Human genes 0.000 description 6
- 108010061982 DNA Ligases Proteins 0.000 description 6
- 102000003960 Ligases Human genes 0.000 description 5
- 108090000364 Ligases Proteins 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 102100031780 Endonuclease Human genes 0.000 description 4
- 101710163270 Nuclease Proteins 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 102000018120 Recombinases Human genes 0.000 description 3
- 108010091086 Recombinases Proteins 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 230000001036 exonucleolytic effect Effects 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108091064358 Holliday junction Proteins 0.000 description 1
- 102000039011 Holliday junction Human genes 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108010020713 Tth polymerase Proteins 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0118959A GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
| EP02741612.2A EP1423532B1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| CA002455803A CA2455803A1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| JP2003517293A JP2004536617A (ja) | 2001-08-03 | 2002-07-12 | 核酸増幅方法 |
| EP10003586.4A EP2251439B1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| EP10003679.7A EP2236622B1 (en) | 2001-08-03 | 2002-07-12 | Method for generating circularised nucleic acid |
| PCT/SE2002/001378 WO2003012119A2 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| AU2002314693A AU2002314693A1 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| EP10003678A EP2224016A1 (en) | 2001-08-03 | 2002-07-12 | Method for generating a circularised nucleic acid |
| US10/483,900 US7320860B2 (en) | 2001-08-03 | 2002-07-12 | Nucleic acid amplification method |
| US11/946,706 US7790388B2 (en) | 2001-08-03 | 2007-11-28 | Nucleic acid amplification method |
| JP2008316989A JP4559517B2 (ja) | 2001-08-03 | 2008-12-12 | 核酸増幅方法 |
| JP2009230088A JP4675424B2 (ja) | 2001-08-03 | 2009-10-02 | 核酸増幅方法 |
| JP2010125694A JP5313204B2 (ja) | 2001-08-03 | 2010-06-01 | 核酸増幅方法 |
| US13/329,998 USRE44265E1 (en) | 2001-08-03 | 2011-12-19 | Nucleic acid amplification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0118959A GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0118959D0 GB0118959D0 (en) | 2001-09-26 |
| GB2378245A true GB2378245A (en) | 2003-02-05 |
Family
ID=9919749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0118959A Withdrawn GB2378245A (en) | 2001-08-03 | 2001-08-03 | Nucleic acid amplification method |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7320860B2 (enExample) |
| EP (4) | EP2236622B1 (enExample) |
| JP (4) | JP2004536617A (enExample) |
| AU (1) | AU2002314693A1 (enExample) |
| CA (1) | CA2455803A1 (enExample) |
| GB (1) | GB2378245A (enExample) |
| WO (1) | WO2003012119A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079042A1 (en) * | 2013-11-29 | 2015-06-04 | Q-Linea Ab | Rolling circle amplification method |
Families Citing this family (121)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| GB0304371D0 (en) * | 2003-02-26 | 2003-04-02 | Solexa Ltd | DNA Sequence analysis |
| US7385043B1 (en) * | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
| SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| WO2005113804A1 (en) * | 2004-05-20 | 2005-12-01 | Trillion Genomics Limited | Use of mass labelled probes to detect target nucleic acids using mass spectrometry |
| WO2006020775A2 (en) * | 2004-08-13 | 2006-02-23 | Harvard University | An ultra high-throughput opti-nanopore dna readout platform |
| WO2006063355A2 (en) * | 2004-12-11 | 2006-06-15 | Cytogenix , Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| WO2007145612A1 (en) * | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Paired end sequencing |
| JP5103398B2 (ja) * | 2005-06-06 | 2012-12-19 | ４５４ ライフ サイエンシーズ コーポレイション | 両末端配列決定（ｐａｉｒｅｄｅｎｄｓｅｑｕｅｎｃｉｎｇ） |
| DK1907571T3 (en) | 2005-06-15 | 2017-08-21 | Complete Genomics Inc | NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS |
| US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
| US8492098B2 (en) | 2006-02-21 | 2013-07-23 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of reaction components that affect a reaction |
| SG170028A1 (en) | 2006-02-24 | 2011-04-29 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090075343A1 (en) | 2006-11-09 | 2009-03-19 | Complete Genomics, Inc. | Selection of dna adaptor orientation by nicking |
| US7862999B2 (en) | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
| WO2008122960A2 (en) * | 2007-04-10 | 2008-10-16 | Microvacuum Ltd. | Label-free optical detection method |
| US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
| EA201000427A1 (ru) * | 2007-10-04 | 2010-10-29 | Хэлсион Молекулар | Секвенирование нуклеиново-кислотных полимеров с использованием электронной микроскопии |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| WO2009073629A2 (en) | 2007-11-29 | 2009-06-11 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| FR2924440B1 (fr) | 2007-12-04 | 2015-01-09 | Pf Medicament | Nouveau procede de generation et de criblage d'une banque d'anticorps |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US20090233809A1 (en) * | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
| US20110159499A1 (en) * | 2009-11-25 | 2011-06-30 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| EP2438442B1 (en) | 2008-12-01 | 2017-08-09 | Laboratory Corporation of America Holdings | Methods and assays for measuring p95 and/or p95 complexes in a sample and antibodies specific for p95 |
| BRPI1007321A2 (en) | 2009-01-15 | 2018-07-10 | Laboratory Corporation Of America Holdings | methods for measuring and / or quantifying the presence and / or amount of her-3 or her-3 in a complex in a patient sample, and to determine if an individual with cancer is likely to respond to treatment with a targeted therapy. and antibody. |
| US20100233732A1 (en) * | 2009-01-15 | 2010-09-16 | Laboratory Corporation Of America Holdings | Methods of Determining Patient Response By Measurement of HER-2 Expression |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| EP2425240A4 (en) | 2009-04-30 | 2012-12-12 | Good Start Genetics Inc | METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| WO2011109379A1 (en) | 2010-03-01 | 2011-09-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| EP2585593B1 (en) | 2010-06-24 | 2017-03-01 | Population Genetics Technologies Ltd. | Methods for polynucleotide library production, immortalization and region of interest extraction |
| WO2012049316A1 (en) | 2010-10-15 | 2012-04-19 | Olink Ab | Dynamic range methods |
| CN101974638B (zh) * | 2010-11-10 | 2012-10-03 | 华东医学生物技术研究所 | 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法 |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| EP3483285B1 (en) | 2011-02-09 | 2021-07-14 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| JP5881746B2 (ja) | 2011-02-15 | 2016-03-09 | ライカ バイオシステムズ ニューキャッスル リミテッド | ｍＲＮＡの局在インサイチュー検出のための方法 |
| US9556473B2 (en) | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
| US10597701B2 (en) | 2011-05-11 | 2020-03-24 | Navinci Diagnostics Ab | Unfolding proximity probes and methods for the use thereof |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| EP2768983A4 (en) | 2011-10-17 | 2015-06-03 | Good Start Genetics Inc | METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES |
| EP3287531B1 (en) | 2012-02-28 | 2019-06-19 | Agilent Technologies, Inc. | Method for attaching a counter sequence to a nucleic acid sample |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| KR101922124B1 (ko) | 2012-08-27 | 2018-11-26 | 삼성전자주식회사 | 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법 |
| US20160369321A1 (en) | 2012-11-14 | 2016-12-22 | Olink Ab | RCA Reporter Probes and Their Use in Detecting Nucleic Acid Molecules |
| US10174366B2 (en) | 2012-11-14 | 2019-01-08 | Olink Bioscience Ab | Localised RCA-based amplification method |
| JP6112652B2 (ja) * | 2012-11-29 | 2017-04-12 | 国立大学法人広島大学 | 標的ｄｎａの特異的増幅方法 |
| ES2900102T3 (es) * | 2013-02-20 | 2022-03-15 | Univ Emory | Composiciones para la secuenciación de ácidos nucleicos en mezclas |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CN117288934A (zh) | 2013-03-13 | 2023-12-26 | 中尺度技术有限责任公司 | 改进的测定方法 |
| EP2971159B1 (en) | 2013-03-14 | 2019-05-08 | Molecular Loop Biosolutions, LLC | Methods for analyzing nucleic acids |
| CN105209532B (zh) | 2013-03-15 | 2017-11-28 | 路博润先进材料公司 | 不含重金属的cpvc组合物 |
| WO2014197377A2 (en) | 2013-06-03 | 2014-12-11 | Good Start Genetics, Inc. | Methods and systems for storing sequence read data |
| CN105555972B (zh) | 2013-07-25 | 2020-07-31 | 伯乐生命医学产品有限公司 | 遗传测定 |
| US9217167B2 (en) | 2013-07-26 | 2015-12-22 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| US9644232B2 (en) | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| US11041203B2 (en) | 2013-10-18 | 2021-06-22 | Molecular Loop Biosolutions, Inc. | Methods for assessing a genomic region of a subject |
| GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
| GB2520765A (en) | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Multiplex detection of nucleic acids |
| US10112194B2 (en) | 2014-04-14 | 2018-10-30 | Q-Linea Ab | Detection of microscopic objects |
| WO2015175530A1 (en) | 2014-05-12 | 2015-11-19 | Gore Athurva | Methods for detecting aneuploidy |
| KR102757668B1 (ko) | 2014-05-15 | 2025-01-21 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| WO2015189390A1 (en) | 2014-06-13 | 2015-12-17 | Q-Linea Ab | Method for detecting and characterising a microorganism |
| GB201413718D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| GB201413717D0 (en) | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| WO2016025818A1 (en) | 2014-08-15 | 2016-02-18 | Good Start Genetics, Inc. | Systems and methods for genetic analysis |
| US11408024B2 (en) | 2014-09-10 | 2022-08-09 | Molecular Loop Biosciences, Inc. | Methods for selectively suppressing non-target sequences |
| US10429399B2 (en) | 2014-09-24 | 2019-10-01 | Good Start Genetics, Inc. | Process control for increased robustness of genetic assays |
| EP4098753A1 (en) | 2014-10-23 | 2022-12-07 | Ricardo Mancebo | Reagents and methods for isothermal chain reaction |
| EP3271480B8 (en) | 2015-01-06 | 2022-09-28 | Molecular Loop Biosciences, Inc. | Screening for structural variants |
| JP6929778B2 (ja) | 2015-01-15 | 2021-09-01 | グッド スタート ジェネティクス， インコーポレイテッド | 着床前遺伝子スクリーニングにおける一塩基多型を用いた品質管理方法 |
| GB201507026D0 (en) | 2015-04-24 | 2015-06-10 | Linea Ab Q | Medical sample transportation container |
| EP3303377B1 (en) | 2015-06-05 | 2022-11-23 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for transient gene therapy with enhanced stability |
| WO2016201142A1 (en) | 2015-06-09 | 2016-12-15 | Life Technologies Corporation | Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging |
| GB201511129D0 (en) | 2015-06-24 | 2015-08-05 | Linea Ab Q | Method of determining antimicrobial susceptibility of a microorganism |
| CN108350500A (zh) | 2015-07-29 | 2018-07-31 | 普罗格尼迪公司 | 用于检测染色体异常的核酸和方法 |
| GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| EP3377647B1 (en) | 2015-11-16 | 2020-03-11 | Progenity, Inc. | Nucleic acids and methods for detecting methylation status |
| WO2017173279A1 (en) * | 2016-03-31 | 2017-10-05 | Arizona Board Of Regents On Behalf Of Arizona State University | Probes and methods for measuring tandem repeats |
| GB2554767A (en) | 2016-04-21 | 2018-04-11 | Q Linea Ab | Detecting and characterising a microorganism |
| US10655170B2 (en) | 2016-07-06 | 2020-05-19 | Takara Bio Usa, Inc. | Coupling adaptors to a target nucleic acid |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| GB201621514D0 (en) | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
| US11661624B2 (en) | 2017-03-30 | 2023-05-30 | Pioneer Hi-Bred International, Inc. | Methods of identifying and characterizing gene editing variations in nucleic acids |
| AU2018251187B2 (en) | 2017-04-14 | 2024-03-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for transient gene therapy with enhanced stability |
| WO2018217905A1 (en) * | 2017-05-23 | 2018-11-29 | Rutgers, The State University Of New Jersey | Target mediated in situ signal amplification with dual interacting hairpin probes |
| JP2020532976A (ja) | 2017-09-14 | 2020-11-19 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 環状一本鎖ｄｎａライブラリーを生成するための新規な方法 |
| EP3682027B1 (en) | 2017-09-15 | 2025-02-26 | F. Hoffmann-La Roche AG | Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries |
| WO2019068797A1 (en) | 2017-10-06 | 2019-04-11 | F. Hoffmann-La Roche Ag | CIRCULARIZATION METHODS FOR THE PREPARATION OF SAMPLE SAMPLES OF UNIQUE MOLECULES |
| WO2019068880A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | RNA MATRIX LIGATION |
| WO2019086531A1 (en) | 2017-11-03 | 2019-05-09 | F. Hoffmann-La Roche Ag | Linear consensus sequencing |
| CN111801427B (zh) | 2018-02-05 | 2023-12-05 | 豪夫迈·罗氏有限公司 | 用于单分子的单链环状dna模板的产生 |
| WO2019166565A1 (en) | 2018-03-02 | 2019-09-06 | F. Hoffmann-La Roche Ag | Generation of double-stranded dna templates for single molecule sequencing |
| EP3775272B1 (en) | 2018-04-02 | 2025-07-30 | Enumera Molecular, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| CN109444102B (zh) * | 2018-12-18 | 2021-03-23 | 济南大学 | 一种检测赭曲霉毒素a的荧光生物传感器及其制备方法和应用 |
| CN120098057A (zh) | 2019-03-01 | 2025-06-06 | 中尺度技术有限责任公司 | 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒 |
| WO2020206170A1 (en) | 2019-04-02 | 2020-10-08 | Progenity, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| JP7727628B2 (ja) | 2019-12-03 | 2025-08-21 | アラマー バイオサイエンシーズ， インク． | 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ） |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| US11492662B2 (en) | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| US11434525B2 (en) | 2020-08-06 | 2022-09-06 | Singular Genomics Systems, Inc. | Spatial sequencing |
| WO2022056078A1 (en) * | 2020-09-11 | 2022-03-17 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Rnase h-assisted detection assay for rna (radar) |
| WO2022061305A1 (en) | 2020-09-21 | 2022-03-24 | Progenity, Inc. | Compositions and methods for isolation of cell-free dna |
| US12497651B2 (en) | 2020-11-23 | 2025-12-16 | Pleno, Inc. | Encoded dual-probe endonuclease assays |
| WO2022235764A1 (en) | 2021-05-05 | 2022-11-10 | Singular Genomics Systems, Inc. | Multiomic analysis device and methods of use thereof |
| US12704508B2 (en) | 2021-06-02 | 2026-08-11 | Alamar Biosciences, Inc. | Nucleic acid linked immune-sandwich assay (NULISA) |
| AU2022304570A1 (en) | 2021-06-28 | 2024-01-18 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for assay signal amplification |
| GB202203185D0 (en) | 2022-03-08 | 2022-04-20 | Rarity Bioscience Ab | Method for detecting nucleic acid amplification products |
| EP4720332A1 (en) | 2023-06-01 | 2026-04-08 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| GB202310278D0 (en) | 2023-07-05 | 2023-08-16 | Navinci Diagnostics Ab | Stabilisation of phi29 polymerase |
| CN120249442B (zh) * | 2025-06-04 | 2025-09-26 | 北京航空航天大学杭州创新研究院 | 一种基于邻近核酸扩增信号放大的超灵敏免疫检测方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992001813A1 (en) * | 1990-07-25 | 1992-02-06 | Syngene, Inc. | Circular extension for generating multiple nucleic acid complements |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| JP3085409B2 (ja) | 1991-03-29 | 2000-09-11 | 東洋紡績株式会社 | 標的核酸配列の検出方法およびそのための試薬キット |
| US5714320A (en) | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US6077668A (en) * | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
| AU8102694A (en) | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
| DE69528670T2 (de) | 1994-12-23 | 2004-03-11 | Dade Behring Inc., Deerfield | Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung |
| SE504798C2 (sv) | 1995-06-16 | 1997-04-28 | Ulf Landegren | Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten |
| DK0862656T3 (da) | 1995-11-21 | 2001-04-09 | Univ Yale | Unimolekylær segmentamplifikation og -detektering |
| DE69636080T2 (de) | 1995-12-05 | 2006-11-30 | Erland Jorn Koch | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| WO1999049079A1 (en) | 1998-03-25 | 1999-09-30 | Ulf Landegren | Rolling circle replication of padlock probes |
| US6037130A (en) | 1998-07-28 | 2000-03-14 | The Public Health Institute Of The City Of New York, Inc. | Wavelength-shifting probes and primers and their use in assays and kits |
| US6150112A (en) | 1998-09-18 | 2000-11-21 | Yale University | Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors |
| US6235502B1 (en) * | 1998-09-18 | 2001-05-22 | Molecular Staging Inc. | Methods for selectively isolating DNA using rolling circle amplification |
| US6830884B1 (en) * | 1998-12-15 | 2004-12-14 | Molecular Staging Inc. | Method of amplification |
| JP4163386B2 (ja) | 1998-12-15 | 2008-10-08 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 環状化核酸プローブの増幅方法 |
| US6274320B1 (en) * | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| JP2003515149A (ja) * | 1999-11-26 | 2003-04-22 | キュラジェン コーポレイション | 核酸プローブアレイ |
| EP1238105A2 (en) | 1999-12-02 | 2002-09-11 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
| US6221603B1 (en) * | 2000-02-04 | 2001-04-24 | Molecular Dynamics, Inc. | Rolling circle amplification assay for nucleic acid analysis |
| US6291187B1 (en) * | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
| US6573051B2 (en) * | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| SE0401270D0 (sv) | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
-
2001
- 2001-08-03 GB GB0118959A patent/GB2378245A/en not_active Withdrawn
-
2002
- 2002-07-12 EP EP10003679.7A patent/EP2236622B1/en not_active Expired - Lifetime
- 2002-07-12 EP EP10003678A patent/EP2224016A1/en not_active Withdrawn
- 2002-07-12 US US10/483,900 patent/US7320860B2/en not_active Expired - Lifetime
- 2002-07-12 CA CA002455803A patent/CA2455803A1/en not_active Abandoned
- 2002-07-12 JP JP2003517293A patent/JP2004536617A/ja active Pending
- 2002-07-12 EP EP10003586.4A patent/EP2251439B1/en not_active Expired - Lifetime
- 2002-07-12 WO PCT/SE2002/001378 patent/WO2003012119A2/en not_active Ceased
- 2002-07-12 AU AU2002314693A patent/AU2002314693A1/en not_active Abandoned
- 2002-07-12 EP EP02741612.2A patent/EP1423532B1/en not_active Expired - Lifetime
-
2007
- 2007-11-28 US US11/946,706 patent/US7790388B2/en not_active Ceased
-
2008
- 2008-12-12 JP JP2008316989A patent/JP4559517B2/ja not_active Expired - Lifetime
-
2009
- 2009-10-02 JP JP2009230088A patent/JP4675424B2/ja not_active Expired - Lifetime
-
2010
- 2010-06-01 JP JP2010125694A patent/JP5313204B2/ja not_active Expired - Lifetime
-
2011
- 2011-12-19 US US13/329,998 patent/USRE44265E1/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992001813A1 (en) * | 1990-07-25 | 1992-02-06 | Syngene, Inc. | Circular extension for generating multiple nucleic acid complements |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
Non-Patent Citations (3)
| Title |
|---|
| Biotechniques; Vol 30 (3), pp 584-588 (2001). Myer & Day * |
| Nucl Acid Res; Vol 26 (22), pp 5073-5078 (1998). Banôr et al. * |
| Nucl Acid Res; Vol 28 (12), pp E58 (2000). Antson et al. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079042A1 (en) * | 2013-11-29 | 2015-06-04 | Q-Linea Ab | Rolling circle amplification method |
| US10227632B2 (en) | 2013-11-29 | 2019-03-12 | Q-Linea Ab | Rolling circle amplification method |
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| WO2003012119A3 (en) | 2003-11-13 |
| EP2251439A1 (en) | 2010-11-17 |
| EP1423532A2 (en) | 2004-06-02 |
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| US7790388B2 (en) | 2010-09-07 |
| GB0118959D0 (en) | 2001-09-26 |
| EP2236622B1 (en) | 2017-09-06 |
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| JP4559517B2 (ja) | 2010-10-06 |
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| US20080131899A1 (en) | 2008-06-05 |
| US7320860B2 (en) | 2008-01-22 |
| EP2224016A1 (en) | 2010-09-01 |
| JP5313204B2 (ja) | 2013-10-09 |
| WO2003012119A2 (en) | 2003-02-13 |
| JP4675424B2 (ja) | 2011-04-20 |
| JP2009077735A (ja) | 2009-04-16 |
| EP2236622A3 (en) | 2010-11-03 |
| JP2009297045A (ja) | 2009-12-24 |
| EP2236622A2 (en) | 2010-10-06 |
| EP2251439B1 (en) | 2013-09-11 |
| EP1423532B1 (en) | 2013-05-22 |
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