GB1590736A - Chemical assay method - Google Patents
Chemical assay method Download PDFInfo
- Publication number
- GB1590736A GB1590736A GB3497477A GB3497477A GB1590736A GB 1590736 A GB1590736 A GB 1590736A GB 3497477 A GB3497477 A GB 3497477A GB 3497477 A GB3497477 A GB 3497477A GB 1590736 A GB1590736 A GB 1590736A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glycerol
- reagent composition
- glycerophosphate
- composition
- lipase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000126 substance Substances 0.000 title claims description 27
- 238000003556 assay Methods 0.000 title description 24
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 215
- 238000000034 method Methods 0.000 claims description 88
- 239000000203 mixture Substances 0.000 claims description 82
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 50
- 102000004316 Oxidoreductases Human genes 0.000 claims description 42
- 108090000854 Oxidoreductases Proteins 0.000 claims description 42
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 39
- 239000004367 Lipase Substances 0.000 claims description 38
- 102000004882 Lipase Human genes 0.000 claims description 37
- 108090001060 Lipase Proteins 0.000 claims description 37
- 235000019421 lipase Nutrition 0.000 claims description 37
- 239000003153 chemical reaction reagent Substances 0.000 claims description 32
- 150000001875 compounds Chemical class 0.000 claims description 22
- 150000003626 triacylglycerols Chemical class 0.000 claims description 22
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 21
- 230000008859 change Effects 0.000 claims description 21
- 230000007062 hydrolysis Effects 0.000 claims description 21
- 238000006460 hydrolysis reaction Methods 0.000 claims description 21
- 239000001301 oxygen Substances 0.000 claims description 21
- 229910052760 oxygen Inorganic materials 0.000 claims description 21
- 239000004094 surface-active agent Substances 0.000 claims description 21
- 239000004365 Protease Substances 0.000 claims description 20
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 17
- 102000057621 Glycerol kinases Human genes 0.000 claims description 15
- 108700016170 Glycerol kinases Proteins 0.000 claims description 15
- 108091005804 Peptidases Proteins 0.000 claims description 14
- 230000003617 peroxidasic effect Effects 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 12
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- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims description 11
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 11
- 239000002243 precursor Substances 0.000 claims description 11
- -1 polyoxyethylene chain Polymers 0.000 claims description 10
- 241000194032 Enterococcus faecalis Species 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
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- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 5
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- 241000894007 species Species 0.000 claims description 5
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000589220 Acetobacter Species 0.000 claims description 3
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- 235000019835 bromelain Nutrition 0.000 claims description 3
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- 238000012986 modification Methods 0.000 claims description 3
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- ZUVBIBLYOCVYJU-UHFFFAOYSA-N naphthalene-1,7-diol Chemical compound C1=CC=C(O)C2=CC(O)=CC=C21 ZUVBIBLYOCVYJU-UHFFFAOYSA-N 0.000 claims description 3
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- 125000004432 carbon atom Chemical group C* 0.000 claims 2
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 2
- FBWNMEQMRUMQSO-UHFFFAOYSA-N tergitol NP-9 Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 FBWNMEQMRUMQSO-UHFFFAOYSA-N 0.000 claims 2
- SZXKSDXHODZTFS-UHFFFAOYSA-N 4-[4,5-bis[4-(dimethylamino)phenyl]-1H-imidazol-2-yl]-2,6-dimethoxyphenol Chemical compound COC1=C(O)C(OC)=CC(C=2NC(=C(N=2)C=2C=CC(=CC=2)N(C)C)C=2C=CC(=CC=2)N(C)C)=C1 SZXKSDXHODZTFS-UHFFFAOYSA-N 0.000 claims 1
- WMVPFEQOLJSBDR-UHFFFAOYSA-N 4-propan-2-yloxynaphthalen-1-ol Chemical compound C1=CC=C2C(OC(C)C)=CC=C(O)C2=C1 WMVPFEQOLJSBDR-UHFFFAOYSA-N 0.000 claims 1
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- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 10
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 10
- 239000000758 substrate Substances 0.000 description 9
- 238000011534 incubation Methods 0.000 description 8
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 7
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- 238000007254 oxidation reaction Methods 0.000 description 7
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 6
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical class CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 5
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- 238000004458 analytical method Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 4
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
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- 230000036284 oxygen consumption Effects 0.000 description 4
- RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 4
- JZLLRGCJEXHGNF-UHFFFAOYSA-M potassium;2-aminoacetic acid;hydroxide Chemical compound [OH-].[K+].NCC(O)=O JZLLRGCJEXHGNF-UHFFFAOYSA-M 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 4
- 101710088194 Dehydrogenase Proteins 0.000 description 3
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- WDGFFVCWBZVLCE-UHFFFAOYSA-N purpurogallin Chemical group C1=CC=C(O)C(=O)C2=C1C=C(O)C(O)=C2O WDGFFVCWBZVLCE-UHFFFAOYSA-N 0.000 description 3
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- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 2
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- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 2
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- 239000002736 nonionic surfactant Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71579776A | 1976-08-19 | 1976-08-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1590736A true GB1590736A (en) | 1981-06-10 |
Family
ID=24875522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3497477A Expired GB1590736A (en) | 1976-08-19 | 1977-08-19 | Chemical assay method |
Country Status (5)
| Country | Link |
|---|---|
| JP (3) | JPS6012040B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1100023A (enrdf_load_stackoverflow) |
| DE (1) | DE2737288C2 (enrdf_load_stackoverflow) |
| FR (1) | FR2362395A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1590736A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894668A (zh) * | 2022-05-11 | 2022-08-12 | 山东省分析测试中心 | 一种基于液体粘度变化的脂肪酶可视化检测方法及检测试剂盒 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5545616B2 (enrdf_load_stackoverflow) * | 1973-05-26 | 1980-11-19 | ||
| US4338395A (en) * | 1980-07-21 | 1982-07-06 | Technicon Instruments Corporation | Method for the analysis of triglycerides |
| JPS5783287A (en) * | 1980-11-14 | 1982-05-25 | Kyowa Hakko Kogyo Co Ltd | Elimination of hydrogen peroxide |
| JPS5794653A (en) * | 1980-12-04 | 1982-06-12 | Konishiroku Photo Ind Co Ltd | Element for analysis |
| JPS5794656A (en) * | 1980-12-04 | 1982-06-12 | Konishiroku Photo Ind Co Ltd | Element for analysis |
| JPS60126084A (ja) * | 1983-12-13 | 1985-07-05 | Toyo Jozo Co Ltd | グリセロリン酸オキシダーゼの安定化法 |
| JP2562882B2 (ja) * | 1986-12-02 | 1996-12-11 | 株式会社シノテスト | 生体成分測定用試薬の安定化法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2000127C3 (de) * | 1970-01-02 | 1974-12-12 | Boehringer Mannheim Gmbh | Verfahren zur quantitativen Spaltung und zum quantitativen Nachweis von Tri-, Di- und Monoglyceriden |
| US3703591A (en) * | 1970-12-16 | 1972-11-21 | Calbiochem | Triglyceride hydrolysis and assay |
| CH548029A (de) * | 1971-03-30 | 1974-04-11 | Hoffmann La Roche | Mittel zum glucosenachweis. |
| JPS4950990A (enrdf_load_stackoverflow) * | 1972-09-13 | 1974-05-17 | ||
| DE2315501C3 (de) * | 1973-03-28 | 1980-02-21 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Bestimmung von Cholesterin |
| DK678474A (enrdf_load_stackoverflow) * | 1974-02-15 | 1975-10-13 | Hoffmann La Roche | |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
-
1977
- 1977-07-08 CA CA282,354A patent/CA1100023A/en not_active Expired
- 1977-08-18 DE DE19772737288 patent/DE2737288C2/de not_active Expired
- 1977-08-19 GB GB3497477A patent/GB1590736A/en not_active Expired
- 1977-08-19 JP JP9941877A patent/JPS6012040B2/ja not_active Expired
- 1977-08-19 FR FR7725353A patent/FR2362395A1/fr active Granted
-
1981
- 1981-05-13 JP JP7213081A patent/JPS5726600A/ja active Granted
-
1986
- 1986-01-24 JP JP1227086A patent/JPH0246200B2/ja not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894668A (zh) * | 2022-05-11 | 2022-08-12 | 山东省分析测试中心 | 一种基于液体粘度变化的脂肪酶可视化检测方法及检测试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5324892A (en) | 1978-03-08 |
| JPS61293397A (ja) | 1986-12-24 |
| DE2737288A1 (de) | 1978-02-23 |
| JPS6012040B2 (ja) | 1985-03-29 |
| CA1100023A (en) | 1981-04-28 |
| FR2362395A1 (fr) | 1978-03-17 |
| JPS5726600A (en) | 1982-02-12 |
| DE2737288C2 (de) | 1985-05-02 |
| FR2362395B1 (enrdf_load_stackoverflow) | 1984-02-24 |
| JPH0131880B2 (enrdf_load_stackoverflow) | 1989-06-28 |
| JPH0246200B2 (ja) | 1990-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19970818 |