JPS6012040B2 - トリグリセリドの検出用組成物及び検出方法 - Google Patents
トリグリセリドの検出用組成物及び検出方法Info
- Publication number
- JPS6012040B2 JPS6012040B2 JP9941877A JP9941877A JPS6012040B2 JP S6012040 B2 JPS6012040 B2 JP S6012040B2 JP 9941877 A JP9941877 A JP 9941877A JP 9941877 A JP9941877 A JP 9941877A JP S6012040 B2 JPS6012040 B2 JP S6012040B2
- Authority
- JP
- Japan
- Prior art keywords
- composition
- glycerophosphate
- lipase
- glycerin
- oxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- 244000273928 Zingiber officinale Species 0.000 description 1
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- 230000003143 atherosclerotic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
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- 239000013043 chemical agent Substances 0.000 description 1
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- OIDPCXKPHYRNKH-UHFFFAOYSA-J chrome alum Chemical compound [K]OS(=O)(=O)O[Cr]1OS(=O)(=O)O1 OIDPCXKPHYRNKH-UHFFFAOYSA-J 0.000 description 1
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- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
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- 239000000839 emulsion Substances 0.000 description 1
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- 150000004665 fatty acids Chemical class 0.000 description 1
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- 239000012530 fluid Substances 0.000 description 1
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- 208000019622 heart disease Diseases 0.000 description 1
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- RSAZYXZUJROYKR-UHFFFAOYSA-N indophenol Chemical compound C1=CC(O)=CC=C1N=C1C=CC(=O)C=C1 RSAZYXZUJROYKR-UHFFFAOYSA-N 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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- 150000004694 iodide salts Chemical class 0.000 description 1
- DXTCFKRAUYBHRC-UHFFFAOYSA-L iron(2+);dithiocyanate Chemical compound [Fe+2].[S-]C#N.[S-]C#N DXTCFKRAUYBHRC-UHFFFAOYSA-L 0.000 description 1
- YWBREQLMDUVNIR-UHFFFAOYSA-N iron(2+);hexacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YWBREQLMDUVNIR-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940057428 lactoperoxidase Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 229960004502 levodopa Drugs 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- NXPPAOGUKPJVDI-UHFFFAOYSA-N naphthalene-1,2-diol Chemical compound C1=CC=CC2=C(O)C(O)=CC=C21 NXPPAOGUKPJVDI-UHFFFAOYSA-N 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
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- 238000006395 oxidase reaction Methods 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- WDGFFVCWBZVLCE-UHFFFAOYSA-N purpurogallin Chemical group C1=CC=C(O)C(=O)C2=C1C=C(O)C(O)=C2O WDGFFVCWBZVLCE-UHFFFAOYSA-N 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 150000003232 pyrogallols Chemical class 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 108091016642 steapsin Proteins 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71579776A | 1976-08-19 | 1976-08-19 | |
| US715797 | 1976-08-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5324892A JPS5324892A (en) | 1978-03-08 |
| JPS6012040B2 true JPS6012040B2 (ja) | 1985-03-29 |
Family
ID=24875522
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9941877A Expired JPS6012040B2 (ja) | 1976-08-19 | 1977-08-19 | トリグリセリドの検出用組成物及び検出方法 |
| JP7213081A Granted JPS5726600A (en) | 1976-08-19 | 1981-05-13 | Method and composition for detecting glycerine |
| JP1227086A Expired - Lifetime JPH0246200B2 (ja) | 1976-08-19 | 1986-01-24 | Toriguriseridokenshutsuyososeibutsu |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7213081A Granted JPS5726600A (en) | 1976-08-19 | 1981-05-13 | Method and composition for detecting glycerine |
| JP1227086A Expired - Lifetime JPH0246200B2 (ja) | 1976-08-19 | 1986-01-24 | Toriguriseridokenshutsuyososeibutsu |
Country Status (5)
| Country | Link |
|---|---|
| JP (3) | JPS6012040B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1100023A (enrdf_load_stackoverflow) |
| DE (1) | DE2737288C2 (enrdf_load_stackoverflow) |
| FR (1) | FR2362395A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1590736A (enrdf_load_stackoverflow) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5545616B2 (enrdf_load_stackoverflow) * | 1973-05-26 | 1980-11-19 | ||
| US4338395A (en) * | 1980-07-21 | 1982-07-06 | Technicon Instruments Corporation | Method for the analysis of triglycerides |
| JPS5783287A (en) * | 1980-11-14 | 1982-05-25 | Kyowa Hakko Kogyo Co Ltd | Elimination of hydrogen peroxide |
| JPS5794653A (en) * | 1980-12-04 | 1982-06-12 | Konishiroku Photo Ind Co Ltd | Element for analysis |
| JPS5794656A (en) * | 1980-12-04 | 1982-06-12 | Konishiroku Photo Ind Co Ltd | Element for analysis |
| JPS60126084A (ja) * | 1983-12-13 | 1985-07-05 | Toyo Jozo Co Ltd | グリセロリン酸オキシダーゼの安定化法 |
| JP2562882B2 (ja) * | 1986-12-02 | 1996-12-11 | 株式会社シノテスト | 生体成分測定用試薬の安定化法 |
| CN114894668B (zh) * | 2022-05-11 | 2025-05-02 | 山东省分析测试中心 | 一种基于液体粘度变化的脂肪酶可视化检测方法及检测试剂盒 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2000127C3 (de) * | 1970-01-02 | 1974-12-12 | Boehringer Mannheim Gmbh | Verfahren zur quantitativen Spaltung und zum quantitativen Nachweis von Tri-, Di- und Monoglyceriden |
| US3703591A (en) * | 1970-12-16 | 1972-11-21 | Calbiochem | Triglyceride hydrolysis and assay |
| CH548029A (de) * | 1971-03-30 | 1974-04-11 | Hoffmann La Roche | Mittel zum glucosenachweis. |
| JPS4950990A (enrdf_load_stackoverflow) * | 1972-09-13 | 1974-05-17 | ||
| DE2315501C3 (de) * | 1973-03-28 | 1980-02-21 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Bestimmung von Cholesterin |
| DK678474A (enrdf_load_stackoverflow) * | 1974-02-15 | 1975-10-13 | Hoffmann La Roche | |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
-
1977
- 1977-07-08 CA CA282,354A patent/CA1100023A/en not_active Expired
- 1977-08-18 DE DE19772737288 patent/DE2737288C2/de not_active Expired
- 1977-08-19 FR FR7725353A patent/FR2362395A1/fr active Granted
- 1977-08-19 JP JP9941877A patent/JPS6012040B2/ja not_active Expired
- 1977-08-19 GB GB3497477A patent/GB1590736A/en not_active Expired
-
1981
- 1981-05-13 JP JP7213081A patent/JPS5726600A/ja active Granted
-
1986
- 1986-01-24 JP JP1227086A patent/JPH0246200B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5726600A (en) | 1982-02-12 |
| JPS61293397A (ja) | 1986-12-24 |
| CA1100023A (en) | 1981-04-28 |
| GB1590736A (en) | 1981-06-10 |
| DE2737288A1 (de) | 1978-02-23 |
| JPH0246200B2 (ja) | 1990-10-15 |
| FR2362395A1 (fr) | 1978-03-17 |
| JPS5324892A (en) | 1978-03-08 |
| FR2362395B1 (enrdf_load_stackoverflow) | 1984-02-24 |
| DE2737288C2 (de) | 1985-05-02 |
| JPH0131880B2 (enrdf_load_stackoverflow) | 1989-06-28 |
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