FI91167B - Ny transaminas, dess framställning och användning - Google Patents

Ny transaminas, dess framställning och användning Download PDF

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Publication number
FI91167B
FI91167B FI892689A FI892689A FI91167B FI 91167 B FI91167 B FI 91167B FI 892689 A FI892689 A FI 892689A FI 892689 A FI892689 A FI 892689A FI 91167 B FI91167 B FI 91167B
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FI
Finland
Prior art keywords
transaminase
carried out
amino group
range
enzyme
Prior art date
Application number
FI892689A
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English (en)
Finnish (fi)
Other versions
FI892689A0 (sv
FI91167C (sv
FI892689A (sv
Inventor
Klaus Sauber
Arno Schulz
Dominique Tripier
Klaus Bartsch
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Hoechst Ag
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Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of FI892689A0 publication Critical patent/FI892689A0/sv
Publication of FI892689A publication Critical patent/FI892689A/sv
Application granted granted Critical
Publication of FI91167B publication Critical patent/FI91167B/sv
Publication of FI91167C publication Critical patent/FI91167C/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Claims (11)

1. Förfarande för framställning av ett transaminas, kannetecknat därav, att man odlar E. coli DH-1 5 (ATCC 33849) och isolerar transaminaset, vars molekylvikt är 25 000 - 100 000 dalton och vars isoelektriska punkt är inom pH-omrädeti 3,5 - 5,5 och vars pH-optimi är inom omrädet: 8,0 - 9,0, och 10 med en substratspecifitet för L-fosfinotricin, γ-amino-smörsyra och glutamat som donator av aminogruppen och motsvarande ketoföreningar som acceptor för aminogruppen.
2. Förfarande enligt patentkravet 1, kannetecknat därav, att odlingen utförs i en temperatur 15 pä 20 - 40 °C.
3. Förfarande enligt patentkravet 2, känne-t e c k n a t därav, att odlingen utförs i en temperatur pä 25 - 37 °C.
4. Förfarande enligt nägot av patentkraven 1-3, 20 kannetecknat därav, att odlingen utförs vid ett pH-värde pä 5 - 8,5.
5. Förfarande enligt patentkravet 4, känne-t e c k n a t därav, att odlingen utförs vid ett pH-värde pä 5,5 - 8,0.
6. Användning av transaminaset erhället enligt pa tentkravet 1 för transaminering av (3-karboxi-3-oxo-pro-py1)-mety1-fos finsyra.
7. Användning enligt patentkravet 6, känne-t e c k n a d därav, att transamineringsreaktionen utförs 30 vid 20 - 60 °C.
8. Användning enligt patentkravet 6 eller 7, kännetecknad därav, att transamineringsreaktionen utförs vid ett pH-värde pä 4 - 12.
9. Användning enligt ett eller flere av patentkra-35 ven 6-8, kännetecknad därav, att transami- naset används i immobiliserad form.
10. Användning enligt patentkravet 9, kanne-t e c k n a d därav, att man som bärarmaterial använder ett kopolymerat ur vinylacetat och divinyletylenurea.
11. Transaminas, kännetecknat därav, att det isolerats ur E. coli DH-1 (ATCC 33849) och att - dess molekylvikt är 25 000 - 100 000 dalton, - dess isoelektriska punkt är inom pH-omrädet: 3,5 - 5,5 10. dess pH-optimi är inom omrädet: 8-9 - det har en substratspecifitet för L-fosfinotri-cin, γ-aminosmörsyra och glutamat som donator av amino-gruppen och motsvarande ketoföreningar som acceptor för aminogruppen. II
FI892689A 1988-06-03 1989-06-01 Ny transaminas, dess framställning och användning FI91167C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3818851 1988-06-03
DE3818851A DE3818851A1 (de) 1988-06-03 1988-06-03 Neue transaminase, ihre herstellung und ihre verwendung

Publications (4)

Publication Number Publication Date
FI892689A0 FI892689A0 (sv) 1989-06-01
FI892689A FI892689A (sv) 1989-12-04
FI91167B true FI91167B (sv) 1994-02-15
FI91167C FI91167C (sv) 1994-05-25

Family

ID=6355745

Family Applications (1)

Application Number Title Priority Date Filing Date
FI892689A FI91167C (sv) 1988-06-03 1989-06-01 Ny transaminas, dess framställning och användning

Country Status (14)

Country Link
US (2) US5130246A (sv)
EP (1) EP0344683B1 (sv)
JP (1) JP2883635B2 (sv)
KR (1) KR0170365B1 (sv)
CN (2) CN1052508C (sv)
AT (1) ATE104342T1 (sv)
AU (1) AU615304B2 (sv)
CA (1) CA1340515C (sv)
DE (2) DE3818851A1 (sv)
DK (1) DK270989A (sv)
ES (1) ES2051921T3 (sv)
FI (1) FI91167C (sv)
IL (1) IL90485A0 (sv)
ZA (1) ZA894179B (sv)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2144999T3 (es) * 1986-06-04 2000-07-01 Hoechst Schering Agrevo Gmbh Procedimiento para la preparacion de l-leucina terciaria mediante transaminacion.
DE3842025A1 (de) * 1988-12-14 1990-07-05 Hoechst Ag Verfahren zur herstellung von l-phosphinothricin
DE3932015A1 (de) 1988-12-15 1991-04-04 Hoechst Ag Gen und genstruktur, codierend fuer eine aminotransferase, mikroorganismen, die dieses gen exprimieren, und transaminierungsverfahren unter verwendung des expressionsprodukts
US4950606A (en) * 1989-06-22 1990-08-21 Celgene Corporation Enantiomeric enrichment and stereoselective synthesis of chiral amines
DE4026628A1 (de) * 1990-08-23 1992-02-27 Hoechst Ag Verfahren zur extraktiven trennung von l-phosphinothricin und l-glutaminsaeure in waessriger loesung
DE4030578A1 (de) 1990-09-27 1992-04-16 Hoechst Ag Verfahren zur herstellung von l-phosphinothricin durch eine gekoppelte enzymatische reaktion
TW198064B (sv) * 1990-12-24 1993-01-11 Hoechst Ag
DE19919848A1 (de) 1999-04-30 2000-11-02 Aventis Cropscience Gmbh Verfahren zur Herstellung von L-Phosphinothricin durch enzymatische Transaminierung mit Aspartat
CN1298860C (zh) * 2004-09-30 2007-02-07 南京大学 γ－氨基丁酸的酶法转化制备方法
CN1295342C (zh) * 2005-03-07 2007-01-17 浙江大学 控制pH发醇生产γ－氨基丁酸的方法
CN101974455B (zh) * 2010-09-28 2012-05-09 郑州大学 高产γ-氨基丁酸的大肠杆菌菌株及其γ-氨基丁酸生产方法
WO2012043653A1 (ja) * 2010-09-28 2012-04-05 株式会社カネカ グルタミン酸に高活性を示す新規アミノ基転移酵素、およびこれをコードする遺伝子、ならびにこれらの利用法
EP3294064A1 (en) 2015-05-11 2018-03-21 Bayer CropScience AG Herbicide combinations comprising l-glufosinate and indaziflam
BR112018067523A8 (pt) 2016-03-02 2023-01-31 Agrimetis Llc Métodos para preparar l-glufosinato
BR112020001585A2 (pt) 2017-07-27 2020-08-11 Basf Se usos de uma composição e método para controlar plantas prejudiciais em uma cultura de campo tolerante a glufosinato
CN110343676B (zh) 2018-04-03 2020-06-23 上海弈柯莱生物医药科技有限公司 一种l-谷氨酸脱氢酶突变体及其应用
US20210214754A1 (en) 2018-09-05 2021-07-15 Basf Se Methods for improving yields of l-glufosinate
CN111019916B (zh) 2018-11-23 2020-12-08 弈柯莱生物科技(上海)股份有限公司 一种d-氨基酸氧化酶突变体及其应用
CN111979208B (zh) 2019-05-23 2023-01-10 弈柯莱生物科技(上海)股份有限公司 一种l-谷氨酸脱氢酶突变体及其应用
US20230082754A1 (en) 2020-01-23 2023-03-16 Basf Se Glufosinate formulations containing amines or ammonium salts
BR112022014824A2 (pt) 2020-01-31 2022-12-13 Basf Se Combinação de herbicidas, composição, métodos de produção de combinações herbicidas, de tratamento ou proteção de plantas produtoras em fileiras e de tratamento ou proteção de plantas especializadas e uso da composição herbicida
WO2021151738A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and epyrifenacil
US20230054749A1 (en) 2020-01-31 2023-02-23 Basf Se Herbicide Combinations Comprising Glufosinate and Flumiclorac-pentyl
BR112022014960A2 (pt) 2020-01-31 2022-09-20 Basf Se Combinação de herbicidas, composição, métodos de produção de uma combinação de herbicidas, para controlar o crescimento de plantas indesejadas, para tratar ou proteger culturas em linha e culturas de especialidades e uso
US20230054333A1 (en) 2020-01-31 2023-02-23 Basf Se Herbicide Combinations Comprising Glufosinate and Saflufenacil
WO2021151745A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and oxadiazon
WO2021151753A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and selected ppo inhibitors
US20230075365A1 (en) 2020-01-31 2023-03-09 Basf Se Herbicide combinations comprising glufosinate and selected ppo inhibitors
WO2021151741A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and tiafenacil
WO2021151744A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and oxyfluorfen
BR112022014727A2 (pt) 2020-01-31 2022-10-11 Basf Se Combinação de herbicidas, composição, métodos para produzir uma combinação de herbicidas, para controlar o crescimento indesejado das plantas, para tratar ou proteger culturas em linha e para tratar ou proteger culturas especiais e uso de uma combinação de herbicidas
BR112022014758A2 (pt) 2020-01-31 2022-10-11 Basf Se Combinação de herbicidas, composição, métodos para produzir uma combinação de herbicidas, para controlar o crescimento indesejado das plantas e para tratar ou proteger culturas e uso da combinação de herbicidas
BR112022014809A2 (pt) 2020-01-31 2022-09-20 Basf Se Combinação de herbicidas, composição, métodos para produzir uma combinação de herbicidas, para controlar o crescimento de plantas indesejadas e para tratar ou proteger culturas e uso de uma combinação de herbicidas
WO2021151737A1 (en) 2020-01-31 2021-08-05 Basf Se Herbicide combinations comprising glufosinate and flumioxazin
EP4000400A1 (en) 2020-11-17 2022-05-25 Basf Se Herbicide combination comprising glufosinate, saflufenacil and trifludimoxazin
WO2022078972A1 (en) 2020-10-12 2022-04-21 Basf Se Herbicide combination comprising glufosinate, saflufenacil and trifludimoxazin
KR20230097101A (ko) 2020-10-27 2023-06-30 바스프 에스이 살충제 마이크로에멀젼 조성물
AR124823A1 (es) 2021-02-05 2023-05-10 Basf Se Composiciones herbicidas líquidas
EP4314310A1 (en) 2021-04-01 2024-02-07 Basf Se Methods for preparing l-glufosinate
CA3219689A1 (en) 2021-05-21 2022-11-24 Anna SOYK Computer-implemented method for applying a glutamine synthetase inhibitor on an agricultural field
CA3238333A1 (en) 2021-11-15 2023-05-19 Dean A Oester Method for increasing the efficacy of a herbicide
WO2024056517A1 (en) 2022-09-14 2024-03-21 Basf Se Use of an alkylether sulfate for improving the efficacy of herbicides
EP4338592A1 (en) 2022-09-15 2024-03-20 Basf Se Use of compound for improving the efficacy of herbicides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525454A (en) * 1983-09-01 1985-06-25 Genetics Institute, Inc. Production of L-4-phenyl-2-aminobutanoic acid by transamination
US4859591A (en) * 1984-07-05 1989-08-22 W. R. Grace & Co.-Conn. Transamination process for producing amino acids
US4826766A (en) * 1985-09-23 1989-05-02 Genetics Institute, Inc. Production of amino acids using coupled aminotransferases
ES2144999T3 (es) * 1986-06-04 2000-07-01 Hoechst Schering Agrevo Gmbh Procedimiento para la preparacion de l-leucina terciaria mediante transaminacion.
AU599985B2 (en) * 1986-06-09 1990-08-02 Meiji Seika Kaisha Ltd. New process for the production of L-2-amino-4- (hydroxymethyl-phosphinyl)-butyric acid
DE3636722A1 (de) * 1986-10-29 1988-05-05 Hoechst Ag Klonierung und verwendung des transaminase-gens ilve
DE3932015A1 (de) * 1988-12-15 1991-04-04 Hoechst Ag Gen und genstruktur, codierend fuer eine aminotransferase, mikroorganismen, die dieses gen exprimieren, und transaminierungsverfahren unter verwendung des expressionsprodukts

Also Published As

Publication number Publication date
CN1038836A (zh) 1990-01-17
FI892689A0 (sv) 1989-06-01
AU3595389A (en) 1989-12-07
FI91167C (sv) 1994-05-25
IL90485A0 (en) 1990-01-18
CN1250101A (zh) 2000-04-12
CA1340515C (en) 1999-04-27
CN1052508C (zh) 2000-05-17
AU615304B2 (en) 1991-09-26
FI892689A (sv) 1989-12-04
JPH0284179A (ja) 1990-03-26
EP0344683A3 (de) 1991-06-19
DE3818851A1 (de) 1989-12-14
EP0344683A2 (de) 1989-12-06
DK270989D0 (da) 1989-06-02
KR0170365B1 (ko) 1999-02-01
JP2883635B2 (ja) 1999-04-19
ZA894179B (en) 1990-02-28
ATE104342T1 (de) 1994-04-15
DE58907431D1 (de) 1994-05-19
US5130246A (en) 1992-07-14
EP0344683B1 (de) 1994-04-13
ES2051921T3 (es) 1994-07-01
US5162212A (en) 1992-11-10
DK270989A (da) 1989-12-04
KR910001036A (ko) 1991-01-30
CN1087347C (zh) 2002-07-10

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Owner name: HOECHST AKTIENGESELLSCHAFT