FI84080B - Foerfarande foer framstaellning av rennin och daeri anvaendbara hybridplasmider. - Google Patents

Foerfarande foer framstaellning av rennin och daeri anvaendbara hybridplasmider. Download PDF

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FI84080B
FI84080B FI820075A FI820075A FI84080B FI 84080 B FI84080 B FI 84080B FI 820075 A FI820075 A FI 820075A FI 820075 A FI820075 A FI 820075A FI 84080 B FI84080 B FI 84080B
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Bernadette Lavetsky Alford
Jen-I Mao
Donald Taylor Moir
Alison Taunton-Rigby
Gerald Francis Vovis
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Collaborative Res Inc
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Claims (11)

1. Förfarande för uttryckning av en gen, som kodar för pre-prorennin eller prorennin, i jäst- eller bakte-rieceller, varvid nukleotidsekvensen och aminosyrasekven-sen av nämnda pre-prorennin är ATG AGG TGT CTC GTG GTG CTA CTT OCT GTC TTC OCT CTC TCC CAG GGC MET ABG CYS LEU VAL VAL LEU LEU ALA VAL PHE ALA LEU SEP, GLN GLY GCT GAG ATC ACC AGG ATC OCT CTC TAC AAA GGC AAG TCT CTG AGG AAG QCG CTG AAG GAG CAT ALA GLU ILE TTO ABG ILE PRO LEU TYR LYS GLY LYS SEP. LED ABG LYS ALA LEU LYS GLU HI S GGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT QGC ATC AGC AGC AAG TAC TCC GGC TTC GLY LEU LEU GLU ASP PHE LEU GLN LYS GLN GLN TYR GLY ILE SEK ££R LYS TYR SER GLY PHE GGG GAG CTG OCC AGC GTG CCC CTG ACC AAC TAC CTG GAT AGT CAG TAC TTT GGG AAG ATC GLY GLU VAI, ALA SER VAL PRO LEU TTO ASN TYR LEU ASP SER GLN TYR PHE GLY LYS ILE TAC CTC GGG ACC CCG OCC CAG GAG TTC ACC CTG CTG TTT GAC ACT GGC TCC TCT GAC TTC TYR LEU GLY TTO PRO PRO GLN GLU PHE THR VAL LEU PHE ASP THR GLY SER SIR ASP PHE TGG CTA CCC TCT ATC TAC TGC AAG AGC AAT CCC TGC AAA AAC CAC CAG OGC TTC GAC CCG TOP VAL PRO SER ILE TYR CYS LYS SER ASN ALA CYS LYS ASN HIS GLN ARG PHE ASP PRO AGA AAG TCG TCC ACC TTC CAG AAC CTG GGC AAG CCC CTG TCT ATC CAC TAC OGG ACA GGC ARG LYS SER SR TTO PHE GLN ASN LEU GLY LYS PRO LEU ST* ILE HIS TYR GLY THR GLY AGC ATG CAG GGC ATC CTG GGC TAT GAC ACC GTC ACT GTC TCC AAC ATT GTG GAC ATC CAG SER (CT GLN GLY ILE LEU GLY TYR ASP THR VAL THR VAL SER ASN ILE VAL ASP ILE GLN CAG ACA CTA GGC CTG AGC ACC CAG GAG OCC OGG GAC GTC TTC ACC TAT OCC GAA TTC GAC GLN TTO VAL GLY LEU SIR TTO GLN GLU PRO GLY ASP VAL PHE TPP, TYR ALA GLU PHE ASP GGG ATC CTG GGG ATG OCC TAC OCC TCG CTC CCC TCA GAG TAC TCG ATA OCC GTG TTT GAC GLY ILE LEU GLY RET ALA TYR PRO SER LEU ALA SER GLU TYR SER ILE PRO VAL PHE ASP AAC ATG ATG AAC AGG CAC CTG GTG OCC CAA GAC CTG TTC TCG GTT TAC ATG GAC AGG AAT ASN MET RET ASN ARG HIS LEU VAL ALA GLN ASP LEU PHE SER VAL TYR RET ASP ARG ASN GGC CAG GAG AGC ATG CTC ACG CTG GGG GCC ATC GAC CCG TCC TAC TAC ACA GGG TCC CTG GLY GLN GLU SER RET LED TTO LEU GLY ALA ILE ASP PRO SER TYR TYR THR GLY SR LEU CAC TGG GTG CCC GTG ACA GTG CAG CAG TAC TGG CAG TTC ACT GTG GAC AGT GTC ACC ATC HIS TOP VAL PRO VAL TTO VAI. GLN GLN TYR TOP GLN PHE THR VAL ASP SR VAL TOR ILE AGC OGT GTG GTT GTG GCC TGT GAG GGT GGC TGT CAG GCC ATC CTG GAC ACG GGC ACC TCC SR GLY VAL VAL VAL ALA CYS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY TOR SR AAG CTG GTC GGG CCC AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA GCC ACA. CAG LYS LEU VAL GLY TOO SIR SIR ASP ILE SU ASN ILE GLN Q.N ALA ILE G1,Y ALA THR GLN AAC CAG TAC GAT GAG TTT GAC ATI GAC TGC GAC AAC CTG AQC TAC ATC COC ALT GTG GTC ASN GLN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN LEU SER TYR »ET TOO THR VAL VAL 84080 TIT GAG ATC AAT GQC AM ATG TAC CCA CTG AOC COC TOC GCC TAT AOC AGC CA3 GAC CAG FHE GLU ILE ASN GLY LYS KET TYR PRO LEU THR FRO SER ALA TYR THR SER GLN ASP GLN GOC TTC TGT AOC AGT GOO TTC CAG ACT GAA AAT CAT TOC CAG AM TOG ATC CTG GGG GAT GLY PHE CYS THR EER GLY PHE GLN SR GLU ASN HIS SER GLN LYS TRP ILE LEU GLY ASP GTT TTC ATC CGA GAG TAT TAC AOC GTC TTT GAC AQG GOC AAE AAC CTC GTG GOG CTG OOC VAL FHE ILE ARG GLU TYR TYR SER VAL ΪΉΕ ASP AFG ALA ASN ASN LEU VAL GLY LEU ALA AM GOC ATC TGA LYS ALA ILE eller en annan nukleotidsekvens, som kodar för densamma aminosyrasekvensen som den angivna nukleotidsekvensen av pre-prorennin, eller en nukleotidsekvens, som kodar för aminosyrasekvensen av en sädan funktionell ekvivalent av pre-prorennin, som har väsentligen densamma aminosyrasekvensen som den, som kodas av den angivna nukleotidsekvensen av pre-prorennin, och varvid nukleotidsekvensen och aminosyrasekvensen av det nämnda prorenninet är OCT GAG ATC ACC AGG ATC CCT CTG TAC AAA GGC MG TCT CTG AGG MG QCG CTG MG GAG CAT ALA GLU ILE TIR ARG ILE PRO LEU TYR LYS GLY LYS SER LEU ARG LYS ALA LEU LYS GLU HIS GGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT GGC ATC AGC AGC MG TAC TCC GGC TTC GLY LEU LEU GLU ASP PHE LEU GLN LYS GLN GLN TYR GLY ILE SR SER LYS TYR 90R GLY PHE OGG GAG GTG GCC AGC GTG CCC CTG ACC MC TAC CTG GAT AGT CAG TAC TTT GGG MG ATC GLY GLU VAL ALA SER VAL PRO LEU THR ASN TYR LEU ASP SR GLN TYR PHE GLY LYS ILE TAC CTC GGG ACC OCG OCC CAG GAG TTC ACC GTG CTG TIT GAC ACT GGC TCC TCT GAC TTC TYR LEU GLY TIR FRO PRO GLN GIL PHE TIR VAL LEU PHE ASP THR GLY SR SER ASP PHE OGG CTA OCC TCT ATC TAC TGC MG AGC MT GCC TGC AAA MC CAC CAG CGC TTC GAC OCG TRP VAL PRO SR ILE TYR CYS LYS SER ASN ALA CYS LYS ASN HIS GLN ARG PHE ASP PRO AGA MG TCG TCC ACC TTC CAG MC CTG GGC MG OCC CTG TCT ATC CAC TAC GGG ACA OGC ARG LYS SER SER TIR PHE GLN ASN LEU GLY LYS FRO LEU SR ILE HIS TYR GLY TOR GLY AGC ATG CAG GGC ATC CTG GGC TAT GAC ACC CTC ACT GTC TCC MC ATT CTG GAC ATC CAG SER KET GLN GLY ILE LEU GLY TYR ASP TOR VAL TIR VAL SR ASN ILE VAL ASP ILE GLN CAG ACA GTA GGC CTG AGC ACC CAG GAG CCC GGG GAC GTC TTC ACC TAT GCC GAA TTC GAC GLN THR VAL GLY LEU SER TW GLN GLU PRO GLY ASP VAL PHE THR TYR ALA GLU PHE ASP GGG ATC CTG GGG ATG GCC TAC CCC TCG CTC GCC TEA GAG TAC TCG ATA OCC GTG TTT GAC GLY ILE LEU GLY MET ALA TYR PRO SER LEU ALA SER GLU TYR SER ILE PRO VAL FHE ASP MC ATG ATG MC AGG CAC CTG GTG GCC CAA GAC CTG TTC TCG CTT TAC ATG GAC AGG MT ASN KET KET ASN ARG HIS LEU VAL ALA GLN ASP LEU PHE SER VAL TYR KET ASP ARG ASN GGC CAG GAG AGC ATG CTC ACG CTG OGG GCC ATC GAC OCG TCC TAC TAC ACA GGG TCC CTG GLY GIN GLU SER KET LEU TIR LEU GLY ALA ILE ASP PRO SER TYR TYR TOR GLY SER LEU 84080 CAC TCG GTG CCC GTG AC A GTG CAG CAG TAC TCG CAG TTC ACT GTG GAC ACT GTC ACC ATC HIS TOP VAL PRO VAL TH* VAL GLM GLN TYR TRP GLN PHE THR VAL ASP EER VAL TOR ILE AGC GGT GTG GTT GTG GCC TGT GAG GGT GGC TGT CAG GCC ATC CTG GAC ACG GGC ACC TCC £ER GLY VAL VAL VAL ALA CYS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY TOR SEP AAG CTG GTC GGG OCC AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA GCC ACA CAG LYS LEU VAL GLY PRO SER SEK ASP ILE LEU ASN ILE GLN GLN ALA ILE GLY ALA TOR GLN AAC CAG TAC GAT GAG TIT' GAC ATC GAC TGC GAC AAC CTG AGC TAC ATG CCC ACT GTG GTC ASN GLN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN LEU SER TYR RET PRO THR VAL VAL TTT GAG ATC AAT GGC AAA ATG TAC CCA CTG ACC OCC TCC GCC TAT ACC AGC CAG GAC CAG PHE GLU ILE ASN GLY LY5 RET TYR PRO LEU THR PRO ST* ALA TYR THR SER GLN ASP GLN GGC TTC TGT ACC ACT GGC TTC CAG ACT GAA AAT CAT TOC CAG AAA TOG ATC CTG GGG GAT GLY FHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE LEU GLY ASP GTT TTC ATC CGA GAG TAT TAC AGC GTC TTT GAC AGG GCC AAC AAC CTC GTG GGG CTC GCC VAL FHE ILE APG GLU TYR TYR SER VAL PHE ASP APG ALA ASN ASN LEU VAL GLY LEU ALA AAA GOC ATC TGA LYS ALA ILE eller en annan nukleotidsekvens, som kodar för densamma aminosyrasekvensen som den angivna nukleotidsekvensen av prorennin, eller en nukleotidsekvens, som kodar för aminosyrasekvensen av en sadan funktionell ekvivalent av prorennin, som har väsentligen densamma aminosyrasekvensen som den, som kodas av den angivna nukleotidsekvensen av prorennin, kännetecknat därav, att man isolerar den nSmnda genen, kopplar en genetisk promotornukleotidsekvens till genens 5'-ände, sa att en hybrid-DNA-molekyl bildas, förutsatt, att när man vill ästadkomma uttryckning av prorennin, ligerar man en ATG-kodon till 5'-änden av den ovan definierade nukleotidsekvensen av prorennin, för den nämnda hybrid-DNA-molekylen genom transformering till värdcellerna, och odlar dessa celler.
2. Förfarande enligt patentkravet 1 för uttryckning av pre-prorennin i jäst- eller bakterieceller, kännetecknat dSrav, att man isolerar en DNA-sekvens, som kodar för pre-prorennin , 79 84080 kopplar till 5'-änden av denna sekvens en tran-skriptionspromotor och ett ribosomalt bindningsstSlle och varierar det avständ, som är mellan början av DNA:n, soin kodar för pre-prorennin och DNA-segmentet, som innehäller promotorn och bindningsstSllet, transformerar DNA:n in i värdceller, klonar värdcellerna och väljer de, som har en hög producer ingsförmaga av pre-prorennin.
3. Förfarande enligt patentkravet 1 för uttryckning av prorennin i jäst- eller bakterieceller, känne- t e c k n a t därav, att man isolerar en DNA-sekvens, som kodar för pre-prorennin och har en 5'-ände, eliminerar den del av 5'-änden, som kodar för pro-rennin-prekursorpolypeptiden, ligerar till resten en syntetisk DNA-bit, som i sin 3'-Snde har en translations-initiationskodon, ligerar till sekvensens 5'-ände en transkriptions-promotor och ett ribosomalt bindningsstSlle och varierar det avständ, som Sr mellan början av DNA:n, som kodar för prorennin och DNA-segmentet, som innehäller promotorn och bindningsstSllet, transformerar DNA:n in i vSrdceller, klonar vSrdcellerna och vSljer de, som har en hög. pro-duceringsförmäga av prorennin.
4. Förfarande enligt patentkravet 1, känne-t e c k n a t därav, att man använder Saccharomyces cerevisiae säsom jSst.
5. Förfarande enligt patentkravet 1, känne-t e c k n a t därav, att man använder Escherichia coli säsom bakterie.
6. Hybridplasmid för transformering av jSst- eller bakterieceller, kSnnetecknad därav, att den innehäller ett i de nämnda jäst- eller bakteriecellerna verkando repi i kat ions-in i t iät. i onsstä 11 e, en i de nämnda jäst- eller bakteriecellerna verkande promotor och tili so 84080 den nämnda promotorn operativt ansluten en nukleotidsek- vens av prorennin OCT GAG ATC ACC AGG ATC OCT CTG TAC AAA OGC AAG TCT CTG AGG AAG OCG CTG AAG GAG CAT ALA GLU ILE TW ARG ILE PRO LEU TYR LYS GLY LYS 6ER LEU ARC LYS ALA LEU LYS GLU HIS OGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT GGC ATC AGO AGC AAG TAC TCC GGC 7TC GLY LEU LEU GLU ASP PHE LEU GLN LYS GIN GIN TYR GLY ILE SEK SER LYS TYR SER GLY PHE OGG GAG GTG OCC AGC GTG OCC CTG ACC AAC TAC CTG GAT AGT CAG TAC TIT OGG AAG ATC GLY GLU VAL ALA SER VAL PRO LEU TUI ASN TYR LEU ASP SER GLN TYR PHE GLY LYS ILE TAC CTC OGG ACC OCG CCC CAG GAG TTC ACC GTG CTG TIT GAC ACT GGC TCC TCT GAC TIG TYR LEU GLY THI PRO PRO GLN GLU PHE THR VAL LEU PHE ASP THR GLY SER ASP PHE TGG GTA OCC TCT ATC TAC TGC AAG AGC AAT OCC TGC AAA AAC CAC CAG OGC TTC GAC OCG TRP VAL PRO SER ILE TYR CYS LYS SER ASN ALA CYS LYS ASN HIS GLN ARC PHE ASP PRO AGA AAG ICG TCC ACC TTC CAG AAC CTG OGC AAG CCC CTG TCT ATC CAC TAC GGG fCA OGC ARG LYS SER SER ThFt PHE GLN ASN LEU GLY LYS PRO LEU S)R ILE HIS TYR GLY TOR GLY AGC ATG CAG OGC ATC CTG GGC TAT GAC ACC CTC ACT GTC TCC AAC ATT GTG GAC ATC CAG SEK RET GLN GLY 1L£ LEU GLY TYR ASP THR VAL TTfc VAL SER ASN ILE VAL ASP ILE GLN CAG ACA GTA GGC CTG AGC ACC CAG GAG CCC OGG GAC GTC TTC ACC TAT GCC GAA TTC GAC GLN THR VAL GLY LEU SER TH? GLN GLU PRO GLY ASP VAL PHE THR TYR ALA GUJ PHE ASP OGG ATC CTG OGG ATG GCC TAC CCC TCG CTC GCC TCA GAG TAC TOG ATA CCC GTG TTT GAC GLY ILE LEU GLY RET ALA TYR PRO SER LEU ALA SER GLU TYR SER ILE PRO VAL PHE ASP AAC ATG ATG AAC AGG CAC CTG GTG GCC CAA GAC CTG TTC TCG CTT TAC ATG GAC AGG AAT ASN MET MET ASN ARG HIS LEU VAL ALA GIN ASP LEU PHE SER VAL TYR RET ASP ARG ASN GGC CAG GAG AGC ATG CTC ACG CTG GGG GCC ATC GAC OCG TCC TAC TAC ACA OGG TCC CTG GLY GLN GLU SER RET LEU TRE LEU GLY AIA ILE ASP PRO SER TYR TYR TOR GLY SER LEU CAC TGG GTG CCC GTG ACA GTG CAG CAG TAC TGG CAG TTC ACT GTG GAC AGT GTC ACC ATC HIS TOP VAL PRO VAL TTR VAL GLN GIN TYR TRP GIN PHE THR VAL ASP SER VAL TOR ILE AGC GGT GTG GTT GTG GCC TGT GAG GGT GGC TGT CAG GCC ATC CTG GAC ACG GGC ACC TCC SER GLY VAL VAL VAL ALA CYS GLU GLY GLY CYS GIN ALA ILE LEU ASP THR GLY TOR SER AAG CTG GTC GGG CCC AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA GCC ACA CAG LYE LEU VAL GLY PRO SER SER ASP ILE LEU ASN ILE GLN GIN ALA ILE GLY ALA TOR GLN AAC CAG TAC GAT GAG ITT GAC ATC GAC TGC GAC AAC CTG AGC TAC ATG CCC ACT GTG GTC ASN GIN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN LEU SER TYR RET PRO THR VAL VAL ITT GAG ATC AAT GGC AAA ATG TAC CCA CTG ACC OCC TCC GCC TAT ACC AGC CAG GAC CAG PHE GLU ILE ASN GLY LYS RET TYR PRO LEU THR PRO SER ALA TYR THR SER GLN ASP GIN GGC TTC TGT ACC AGT GOC TTC CAG ACT GAA AAT CAT TCC CAG AAA TGG ATC CTC GGG GAT GLY PHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE LEU GLY ASP GTT TTC ATC CGA GAG TAT TAG AGC GTC TTT GAC AGG GCC AAC AAC CTC GTG GGG CTC GOC VAL PHE ELE ARG GLU TYR TYR SER VAL PHE ASP ARG ALA ASN ASN LEU VAL GLY LEU ALA AAA GOC ATC TGA LYS ALA LLE 81 84080
7. Plasmiden pCGS28, som innehäller en gen, som kodar för prorennin, och är ansluten till Saccharomyces cere-visiae-jäststammen CGY116, ATCC 20623.
8. Hybridplasmid för transformering av jäst- eller bakterieceller, kSnnetecknad därav, att den innehäller ett i de nämnda jäst- eller bakteriecellerna verkande replikations-initiationsställe, en i de nämnda jäst- eller bakteriecellerna verkande promotor och tili den nämnda promotorn operativt ansluten en nukleotidsek-vens av pre-prorennin ATG AGG TGT CTC GTG GTG CTA CTT GOT GTC OTC OCT CTC TCC CAG GGC ►ET AFG CY5 LEU VAL VAL LEU LEU ALA VAL PHE ALA LEU SER GLN GLY GCT GAG ATC ACC AGG ATC OCT CTG TAC AAA GGC AAG TCT CTG AGG AAG GCG CTC AAG GAG CAT AIA GUU ILE TIR ARG ILE PRO LEU TYR LY5 GLY LYS SER LEU ARG LYS ALA LEU LYS GLU Hl S QGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT GGC ATC AGC AGC AAG TAC TCC GGC TTC GLY LEU LEU GLU ASP PHE LEU GIN LYS G LA GLN TYR GLY ILE SER SER LYS TYR SER GLY PHE GGG GAG GTG GCC AGC GTG OCC CTG ACC AAC TAC CTG GAT AGT CAG TAC TTT GGG AAG ATC GLY GLU VAL AIA SER VAL PRO LEU TIR ASN TYR LEU ASP SSR G114 TYR PHE GLY LYS ILE TAC CTC GGG ACC OCG OCC CAG GAG TTC ACC GTG CTG TTT GAC ACT GGC ICC TCT GAC TTC TYR LEU GLY TIR PRO PRO G LM GLU PHE THR VAL LEU PHE ASP THR GLY SER £ER ASP PHE 1GG GTA OCC TCT ATC TAC TGC AAG AGC AAT OCC T3C AAA AAC CAC CAG OGC TTC GAC CCG TRP VAL PRO SER ILE TYR CYS LYS SER ASN AIA CYS LYS ASN HIS GLN ARG PHE ASP PRO AGA AAG TCG TCC ACC TTC CAG AAC CTG GGC AAG OCC CTG TCT ATC CAC TAC GGG ACA GGC ARG LYS £ER SER TIR PHE GLN ASN LEU GLY LYS FRO LEU SER ILE HIS TYR GLY THR GLY AGC ATG CAG GGC ATC CTG GGC TAT GAC ACC GTC ACT GTC TCC AAC ATT GTG GAC ATC CAG SER KET GLN GLY ILE LEU GLY TYR ASP THR VAL THR VAL SER ASN ILE VAL ASP ILE GLN CAG ACA CTA GGC CTG AGC ACC CAG GAG OCC GGG GAC GTC TTC ACC TAT OCC GAA TTC GAC GIN Tl·* VAL GLY LEU SER TW GLN GUU PRO GLY ASP VAL PHE TUS TYR ALA GLU PHE ASP GGG ATC CTG GGG ATG OCC TAC OCC TCG CTC OCC TCA GAG TAC TCG ATA OCC GTG TTT GAC GLY ILE LEU GLY MET ALA TYR PRO SER LEU ALA SER GLU TYR SER ILE PRO VAL PHE ASP AAC ATG ATG AAC AGG CAC CTG GTG GCC CAA GAC CTG TTC TCG GTT TAC ATG GAC AGG AAT ASN KET KET ASN ARG HIS LEU VAL ALA GIN ASP LEU PHE SER VAL TYR MET ASP ARG ASN OGC CAG GAG AGC ATG CTC ACG CTG GGG GCC ATC GAC OCG TCC TAC TAC ACA GGG TCC CTG GLY GIN GLU SER ►ET LEU TIR LEU GLY AIA ILE ASP PRO SER TYR TYR THR GLY S:R LEU CAC TGG GTG OCC GTG ACA GTG CAG CAG TAC TGG CAG TTC ACT CTG GAC AGT OTC ACC ATC HIS TRP VAL PRO VAL TIR VAL GLN GLN TYR TRP GIN PHE THR VAL ASP SER VAL THR ILE 84080 AGC GGT GTG GTT GTG GCC TGT GAG GGT GGC TGT CAG OCC ATC CTG GAC ACG GGC ACC TCC SER GLY VAL VAL VAL ALA CYS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY TOR SER AAG CTG GTC GGG CCC AGC AQC GAC ATC CTC AAC ATC CAG CAG GGC ATT GGA GOC ACA. CAG LYS LEU VAL GLY FRO SER SER ASP ILE LEU ASN ILE GLN <XN ALA ILE GLY ALA THR GLN AAC CAG TAC GAT GAG TTT GAC ATC GAC TOC GAC AAC CFG AGC TAC ATC COC ACT GTG GTC ASN GLN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN LEU SER TYR PET PRO THR VAL VAL ITT GAG ATC AAT GGC AAA ATC TAC CCA CTC ACC CCC TOC GCC TAT AOC AGC CAG GAC CAG HIE GLU ILE ASN GLY LYS PET TYR PRO LEU THR FRO ALA TYR THR SER GLN ASP GLN GOC TIC TGT AOC AGT GGC TTC CAG ACT GAA AAT CAT TOC CAG AAA TOG ATC CTC GGG GAT GLY FHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE LEU GLY ASP GTT TIC ATC CGA GAG TAT TAC AOC GTC TTT GAC AOG GOC AAC AAC CTC GTC GOG CTC GOC VAL FHE ILE ARG GLU TYR TYR SER VAL FHE ASP AFC ALA ASN ASN LEU VAL GLY LEU ALA AAA GCC ATC TGA LYS ALA ILE
9. Hybridplasmid enligt patentkravet 8, k ä n n e -t e c k n a d dSrav, att den är plasmiden pCGE21, an-sluten till Escherichia coli-bakteriestammen CGE24, ATCC 31929.
10. Hybridplasmid för transformering av jäst- eller bakterieceller, kännetecknad därav, att den innehaller ett i de nämnda jäst- eller bakteriecellerna verkande replikations-initiationsställe, en i de nämnda jäst- eller bakteriecellerna verkande promotor och tili den nämnda promotorn operativt ansluten en nukleotidsek-vens av rennin GGG GAG GTG GOC AGC GTC COC CTC AOC AAC TAC CTC GAT ACT CAG TAC TTT GOG AAG ATC GLY GLU VAL ALA SER VAL IHO LEU THR ASN TYR LEU ASP SER GLN TYR PHE GLY LYS ILE TAC CTC GGG ACC COG COC CAG GAG TTC AOC GTC CTG TTT GAC ACT GGC TCC TCT GAC TIC TYR LEU GLY THR ERO ERO GLN GLU PHE THR VAL LEU PHE ASP THR GLY SER SER ASP PHE TGG GTA CCC TCT ATC TAC TQC AAG AGC AAT GOC TOC AAA AAC CAC CAG OQC TTC GAC CCG TOP VAL FRO SER ILE TYR CYS LYS S3* ASN ALA CYS LYS ASN H IS GLN ARG PHE ASP PRO AGA AAG TCG TOC ACC TTC CAG AAC CTC GGC AAG COC CTG TCT ATC CAC TAC GGG ACA GGC AFG LYS SER SER THR PHE GLN ASN LEU GLY LYS FRO LEU SER ILE H1S TYR GLY THR GLY AGC ATC CAG GGC ATC CTG GGC TAT GAC ACC GTC ACT GTC TCC AAC ATT GTC GAC ATC CAG SER PET GLN GLY ILE LEU GLY TYR ASP THR VAL THR VAL SER ASN ILE VAL ASP ILE GLN CAG ACA GTA GGC CTC AGC ACC CAG GAG COC GGG GAC GTC TTC ACC TAT GOC GM TTC GAC GLN THR VAI, GLY LEU SER THR GLN GLU FRO GLY ASP VAL PHE THR TYR ALA GLU FHE ASP GGG ATC CTC GGG ATC GCC TAC COC TCG CTC GCC TCA GAG TAC TCG ATA COC GTC ΤΓΓ GAC GLY ILK LKU GLY PICT AJA TYR IRO SEK 1EU AJA SEK GLU TYR SER IIE FRO VAL PHE AST AAC ATC ATC AAC AQG CAC CTC GTC GOC CAA GAC CTC TTC TCG GTT TAC ATC GAC AGG AAT ASN PET PET ASN ARG H IS LEU VAL ALA GLN ASP LEU FHE SER VAL TYR PET ASP AFG ASN GGC CAG GAG AGC ATC CTC AOG CTC GGG GOC ATC GAC CCG TCC TAC TAC ACA GOC TCC CTC GLY GLN GLU SER PET LEU THR LEU GLY ALA ILE ASP FRO SER TYR TYR THR GLY SER LEU θ3 8 4 O E O etc TOG GTG CGC GTC ACA GTG CAG CAG TAG TOG CA3 TTC ACT GTG GAC ACT GTC ACC ATC H IS TRP VAL PRO VAL THR VhL GLN GLN TYR TRP GLN FHE THR VAL ASP SER VAL THR ILE AGC GGT GIG GTT GTG GOC TGT GAG GGT GGC TGT CAG GOC ATC CTG GAC ACG GGC ACC TCC SER GLY VAL VAL \AL ALA CYS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY THR SER AAG CTG GTC GGG COC AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA GOC ACA CAG LYS IEU VAL GLY PRO a» SER ASP ILE LEU ASN ILE GLN GLN ALA ILE GLY ALA THR GLN AAC CAG TAC GAT GAG TTT GAC ATC GAC TGC GAC AAC CTG AGC TAC ATG COC ACT GTG GTC ASN GLN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN IEU SR TYR F£T PRO THR VAL VAL TTT GAG ATC AAT GGC AAA ATG TAC CCA CTG AOC COC TCC GOC TAT AOC AGC CAG GAC CAG PHE GLU ILE ASN GLY LYS »CT TYR PRO IEU THR PRO SER ALA TYR THR £ER GLN ASP GLN GGC TTC TCT ACC ACT GGC TTC CAG ACT GAA AAT CAT TCC CAG AAA TOG ATC CTG GGG GAT GLY FHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE IEU GLY ASP GTT TTC ATC CGA GAG TAT TAC AGC GTC TTT GAC AGG GOC AAC AAC CTC GTG GGG CTC GCC VAL PHE ILE AFG GLU TYR TYR SER VAL PHE ASP ARG ALA ASN ASN IEU VAL GLY IEU ALA AAA GOC ATC TGA LYS ALA ILE
11. Förfarande för framställning av rennin eller en funktionell ekvivalent därav, kännetecknat därav, att man odlar jäst- eller bakterieceller, som innehäller en hybridplasmid, som innehäller en gen, som kodar för pre-prorennin, som har den följande nukleotid-sekvensen ATG AGG TGT CTC CTG CTG CTA CTT GCT GTC TTC QCT CTC TCC CAG GGC MST ARG CVS LEU VAL VAL LEU LEU ALA VAL PHE ALA LEU SER GLN GLY GCT GAG ATC ACC AGG ATC OCT CTG TAC AAA GGC AAG TCT CTG AGG AAG QCG CTG AAG GAG CAT AIA GLU ILE TIR ARG ILE PRO LEU TYR LYS GLY LYS SER LEU ARG LYS ALA LEU LYS GLU HIS GGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT GGC ATC AGC AGC AAG TAC TCC GGC TTC GLY LEU LEU GLU ASP PHE LEU GLN LYS GIN GIN TYR GLY ILE SER SER LYS TYR SER GLY PHE GGG GAG GTG GCC AGC GTG OCC CTG ACC AAC TAC CTG GAT AGT CAG TAC TTT GGG AAG ATC GLY GLU VAL AIA SER VAL PRO LEU TIR ASN TYR LEU ASP SER GIN TYR FHE GLY LYS ILE TAC CTC GGG ACC CCG OCC CAG GAG TTC ACC CTG CTG TTT GAC ACT GGC TCC TCT GAC TTC TYR LEU GLY TIR PRO PRO GLN GLJU PHE THR VAL LEU PHE ASP THR GLY SER ££R ASP PHE TGG CTA OCC TCT ATC TAC TGC MG AGC AAT GCC TGC AAA MC CAC CAG CGC TTC GAC CCG TOP VAL PRO SER ILE TYR CYS LYS SR ASN AIA CYS LYS ASN HIS GLN ARG PHE ASP PRO AGA MG TCG ICC ACC TTC CAG MC CTG GGC MG OCC CTG TCT ATC CAC TAC GGG ACA GGC ARG LYS SER SER TIR PHE GIN ASN LEU GLY LYS PRO LEU SER ILE HIS TYR GLY THR GLY AGC ATG CAG GGC ATC' CTC GGC TAT GAC ACC GTC ACT GTC TCC AAC ATT CTG GAC ATC CAG SER FET GLN GLY ILE LEU GLY TYR ASP THk VAL THR VAL SER ASN ILE VAL ASP ILE GLN 34 84060 CAG AC A GTA QGC CTG AGC ACC CAG GAG OCC OGG GAC GTC TTC ACC TAT GCC GAA TTC GAC GLN TTO VAL GLY LEU SEK TTO GLN GLU PRO GLY ASP VAL PHE TTO TYR ALA GLU PHE ASP GGG ATC CTG GGG ATG GCC TAC OCC TCG CTC GCC TCA GAG TAC TCG ATA CCC GTG TTT GAC GLY ILE LEU GLY MET ALA TYR PRO SER LEU ALA SEP GLU TYR SEP ILE PRO VAL PHE ASP AAC ATG ATG AAC AGG CAC CTG GTG GCC CAA GAC CTG TTC TCG GTT TAC ATG GAC AGG AAT ASN PET PET ASN APG HIS LEU VAL ALA GLM ASP LEU PHE SER VAL TYR PET ASP ARG ASN GGC CAG GAG AGC ATG CTC ACG CTG GGG GCC ATC GAC OCG ICC TAC TAC ACA GGG TCC CTG GLY GLN GLU ffiR PET LEU TTO LEU GLY ALA ILE ASP PRO SER TYR TYR THR GLY SER LEU CAC TCG CTG CCC GTG ACA GTG CAG CAG TAC TCG CAG TTC ACT GTG GAC AGT GTC ACC ATC HIS TOP VAL PRO VAL TPf< VAL GLN GLN TYR TOP GLN PHE THR VAL ASP S:R VAL THR ILE AGC QGT CTG GTT GTG QCC TGT GAG GGT GGC TCT CAG GCC ATC CTG GAC ACG OGC ACC TCC SER GLY VAL VAL VAL ALA CIS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY TOR SER AAG CTC GTC GGG COE AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA GCC ACA. CAG LYS LEU VAL GLY TOO SER SER ASP ILE LEU ASN ILE GLN GLN ALA ILE GLY ALA THR GLN AAC CAG TAC GAT GAG ΤΤΓ GAC ATC GAC TOC GAC AAC CTC AGC TAC ATC COC ACT GTC GTC ASN GLN TYR ASP GLU RLE ASP ILE ASP CYS ASP ASN LEU SER TYR PET FRO THR VAL VAL TTT GAG ATC AAT GGC AAA ATC TAC CCA CTC ACC COC TOC GCC TAT ACC AGC CAG GAC CAG PHE GLU ILE ASN GLY LYS PET TYR TOO LEU THR TOO SER ALA TYR THR SER GLN ASP GLN GGC TTC TGT ACC ACT GGC TTC CAG ACT GAA AAT CAT TOC CAG AAA TOG ATC CTC GGG GAT GLY FHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE LEU GLY ASP GTT TIC ATC CGA GAG TAT TAC AGC GTC TTT GAC AGG GOC AAC AAC CTC GTC GGG CTC GOC VAL FHE ILE ARG GLU TYR TYR SER VAL ΙΉΕ ASP ARG ALA ASN ASN LEU VAL GLY LEU ALA AAA GOC ATC TGA LYS ALA ILE eller en annan nukleotidsekvens, som kodar för densamma aminosyrasekvensen som den angivna nukleotidsekvensen av pre-prorennin, eller en nukleotidsekvens, som kodar för aminosyrasekvensen av en sadan funktionell ekvivalent av pre-prorennin, som har väsentligen densamma aminosyrasekvensen som den, som kodas av den angivna nukleotidsekvensen av pre-prorennin, eller en gen, som kodar för prorennin, som har den följande nukleotidsekvensen 85 8 4 ο ε o OCT GAG ATC ACC AGG ATC OCT CTG TAC AAA GGC AAG TCT CTG AGG AAG GCG CTG AAG GAG CAT ALA GLU ILE TIR ARG ILE PRO LEU TYR LYS GLY LYS 6ER LEU APG LYS ALA LEU LYS GLU HIS GGG CTT CTG GAG GAC TTC CTG CAG AAA CAG CAG TAT GGC ATC AGC AGC AAG TAC TCC GGC TTC GLY LEU LEU GLU ASP PHE LEU GLN LYS GIN GLN TYR GLY ILE SER SR LYS TYR S3? GLY PHE Q3G GAG GTG GCC AGC GTG OCC CTG ACC AAC TAC CTG GAT ACT CAG TAC ΤΓΤ GGG AAG ATC GLY GLU VAL ALA SER VAL PRO LEU TWt ASN TYR LEU ASP SER GLN TYR PHE GLY LYS ILE TAC CTC QGG ACC OCG OCC CAG GAG TTC ACC GTG CTG TTT GAC ACT GGC TCC TCT GAC TIC TYR LEU GLY Ttf* PRO FRO GLN GLU PHE THR VAL LEU PHE ASP THR GLY SER SER ASP PHE TGG GTA OCC TCT ATC TAC TGC AAG AGC AAT GCC TGC AAA AAC CAC CAG CGC TTC GAC OCG TRP VAL PRO SER ILE TYR CYS LYS SER ASN ALA CVS LYS ASN HIS GLN ARG PHE ASP PRO AGA AAG TCG TCC ACC TTC CAG AAC CTG GGC AAG OCC CTG TCT ATC CAC TAC GGG ACA OX ATC LV5 SR SER TIR PHE GLN ASN LEU GLY LYS FRO LEU SEP ILE HIS TYR GLY TOR GLY AGC ATG CAG GGC ATC CTG GGC TAT GAC ACC CTC ACT GTC TCC AAC ATT GTG GAC ATC CAG SER KET GLN GLY ILE LEU GLY TYR ASP THR VAL TH< VAL SER ASN ILE VAL ASP ILE GLN CAG ACA GTA GGC CTG AGC ACC CAG GAG OCC GGG GAC GTC TTC ACC TAT GCC GAA TTC GAC GLN THR VAL GLY LEU SER TIE GLN GLU PRO GLY ASP VAL PHE THR TYR ALA GLU PHE ASP GGG ATC CTG GGG ATG GCC TAC OCC TCG CTC GCC TCA GAG TAC TCG ATA OCC GTG TTT GAC GLY ILE LEU GLY KET ΑΙΑ TYR PRO SER LEU ALA SER GLU TYR SER ILE PRO VAL PHE ASP AAC ATG ATG AAC AGG CAC CTG GTG GCC CAA GAC CTG TTC TCG GTT TAC ATG GAC PCG AAT ASN KET KET ASN ARG HIS LEU VAL ALA GLN ASP LEU PHE SER VAL TYR KET ASP ARG ASN GGC CAG GAG AGC ATG CTC ACG CTG GGG GCC ATC GAC CCG TCC TAC TAC ACA GGG ICC CTG GLY GIN GLU SER KET LEU THR LEU GLY ALA ILE ASP PRO SIR TYR TYR TOR GLY SER LEU CAC TGG GTG OCC GTG ACA GTG CAG CAG TAC TGG CAG TTC ACT GTG GAC AST GTC ACC ATC HIS TOR VAL PRO VAL TIR VAL GLN GLN TYR TRP GLN PHE THR VAL ASP SR VAL TOR ILE AGC GGT GTG GTT GTG GCC TGT GAG GGT GGC TGT CAG GCC ATC CTG GAC ACG GGC ACC TCC SER GLY VAL VAL VAL ALA CYS GLU GLY GLY CYS GLN ALA ILE LEU ASP THR GLY TOR SER AAG CTG GTC GGG OCC AGC AGC GAC ATC CTC AAC ATC CAG CAG GCC ATT GGA OCC ACA CAG LYS LEU VAL GLY PRO SER SR ASP ILE LEU ASN ILE GLN GLN ALA ILE GLY ALA TOR GLN AAC CAG TAC GAT GAG ITT GAC ATC GAC TGC GAC AAC CTG AGC TAC ATG OCC ACT GTG GTC ASN GLN TYR ASP GLU PHE ASP ILE ASP CYS ASP ASN LEU SER TYR KET PRO THR VAL VAL ΤΓΤ GAG ATC AAT GGC AAA ATG TAC OCA CTG ACC OCC ICC OCC TAT ACC AGC CAG GAC CAG PHE GLU ILE ASN GLY LV5 KET TYR FRO LEU THR PRO SER ALA TYR THR SER GLN ASP GLN GOC TTC TGT ACC ACT GGC TTC CAG AGT GAA ΑΛΤ CAT TCC CAG ΑΛΑ TGG ATC CTG GGG GAT GLY FHE CYS THR SER GLY PHE GLN SER GLU ASN HIS SER GLN LYS TRP ILE IEU GLY ASP GTT TTC ATC CGA GAG TAT TAC AGC GTC TTT GAC AGG GOC AVAC AVAC CTC GTG GGG CTG GOC VAL PHE ILE ARG GLU TYR TYR SER VAL FHE ASP ARG ALA ASN ASN LEU VAL GLY LEU ALA AAA GOC ATC TGA LYS ALA ILE 86 8 4 O o O eller en annan nuk.leotidsek.vens, som kodar för densamma aminosyrasekvensen som den angivna nukleotidsekvensen av prorennin, eller en nukleotidsekvens, som kodar för aminosyrasekvensen av en sädan funktionell ekvivalent av prorennin, som har väsentligen densamma aminosyrasekvensen som den, som kodas av den angivna nukleotidsekvensen av prorennin, tillvaratar pre-prorennin, prorennin eller funktionella ekvivalenter därav frlin de nämnda cellerna och klyver pre-prorennin, prorennin eller en funktionell ekvivalent därav, varvid man erhäller rennin eller en funktionell ekvivalent därav.
FI820075A 1981-01-16 1982-01-11 Förfarande för framställning av rennin och däri användbara hybridplasm ider FI84080C (sv)

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US5525484A (en) * 1981-01-16 1996-06-11 Genome Therapeutics Corp. Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
JPS5832896A (ja) * 1981-08-24 1983-02-25 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
EP0096910B1 (en) * 1982-05-19 1989-04-19 Unilever N.V. Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms
IL68797A0 (en) * 1982-07-01 1983-09-30 Genex Corp Cloned bovine calf chymosin and calf prochymosin genes,their preparation,plasmids containing them and microorganisms transformed by those plasmids
US4935369A (en) * 1982-12-22 1990-06-19 Genentech, Inc. Isolation of active microbially produced bovine rennin
US4961938A (en) * 1982-12-22 1990-10-09 Genentech, Inc. Preparation of cheese with rennin from recombinant microbial cells
IE56372B1 (en) * 1982-12-22 1991-07-03 Genentech Inc Microbially produced rennet methods for its production and plasmids used for its production
US4661454A (en) * 1983-02-28 1987-04-28 Collaborative Research, Inc. GAL1 yeast promoter linked to non galactokinase gene
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法
DE3480006D1 (en) * 1983-05-19 1989-11-09 Unilever Nv Improvements in the expression of newly introduced genes in yeast cells
EP0128743A3 (en) * 1983-06-07 1985-12-27 Genex Corporation Expression of foreign genes in yeast
AU3036684A (en) * 1983-07-07 1985-01-10 Genex Corp. Production of bovine calf chymosin
US4935354A (en) * 1983-07-14 1990-06-19 Genentech, Inc. Rennin from recombinant microbial cells for preparation of cheese
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
US5639639A (en) * 1983-11-02 1997-06-17 Genzyme Corporation Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof
US4935370A (en) * 1983-12-23 1990-06-19 Pfizer Inc. Expression plasmids for improved production of heterologous protein in bacteria
NL8400687A (nl) * 1984-03-02 1985-10-01 Unilever Nv Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
AU4403885A (en) * 1984-05-11 1985-12-13 Codon Genetic Engineering Laboratories An efficient process for producing active chymosin from a precursor protein synthesized in bacteria
GB2178431B (en) * 1985-07-15 1989-11-22 Bioteknika International Genetically engineered yeast strains
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US6004785A (en) * 1985-08-29 1999-12-21 Genencor International Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
JPH0673456B2 (ja) * 1986-04-26 1994-09-21 三共株式会社 ヒト・膵臓エラスタ−ゼ▲ｉ▼
ES2033747T3 (es) * 1986-07-22 1993-04-01 Ciba-Geigy Ag Preparacion de polipeptidos relacionados con factores de ligacion.
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
US5162226A (en) 1987-08-24 1992-11-10 University Of Tennessee Research Corp. (U.T.R.C.) Therapeutic compositions against streptococcal infections, transformed hosts, methods of immunization and genetically engineered products
JPH02109984A (ja) * 1988-12-01 1990-04-23 Teruhiko Beppu 複合プラスミド及びそれを含有する微生物
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
JPH05505308A (ja) * 1990-03-13 1993-08-12 ハワイ・バイオテクノロジー・グループ・インコーポレイテツド アオパンカビ発現システム
IE914007A1 (en) * 1990-11-19 1992-05-20 Univ Illinois A protein being genetically engineered to provide¹orientability and a substrate having an oriented layer of¹proteins
PT700445E (pt) * 1993-06-04 2002-07-31 Whitehead Biomedical Inst Proteinas de stress e suas utilizacoes
US7157089B1 (en) * 1996-11-26 2007-01-02 Stressgen Biotechnologies Corporation Immune responses using compositions containing stress proteins
EP1196772A2 (en) * 1999-07-08 2002-04-17 Stressgen Biotechnologies Corporation Induction of a th1-like response in vitro
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
US6964851B2 (en) * 1999-12-07 2005-11-15 Stressgen Biotechnologies Corp. Compositions and methods for detecting stress-inducible proteins
JP2003519668A (ja) * 2000-01-14 2003-06-24 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ 熱ショックタンパク質融合タンパク質による、ｃｄ４＋ｔ細胞非依存性のインビボｃｔｌ惹起による個別ａｔｐ結合ドメインのマッピング
DK1296711T3 (da) * 2000-06-26 2006-08-28 Stressgen Biotechnologies Corp HPV-E7 til behandling af humant papillomavirus
WO2002062959A2 (en) * 2001-02-05 2002-08-15 Stressgen Biotechnologies Corp. Hepatitis b virus treatment
WO2005094185A2 (en) * 2004-03-30 2005-10-13 Sudershan Biotech Limited Recombinant calf-chymosin and a process for producing the same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4136201A (en) * 1967-12-06 1979-01-23 Gb Fermentation Industries, Inc. Microbial rennin
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
US4332892A (en) * 1979-01-15 1982-06-01 President And Fellows Of Harvard College Protein synthesis
AU542264B2 (en) * 1979-06-01 1985-02-14 G.D. Searle & Co. Plasmid vectors
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide

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EP0057350A2 (en) 1982-08-11
AU560340B2 (en) 1987-04-02
IE52760B1 (en) 1988-02-17
DE3280479D1 (de) 1998-11-26
EP0057350A3 (en) 1982-12-01
NZ199445A (en) 1985-07-12
IE820069L (en) 1983-06-01
BR8200191A (pt) 1982-11-09
JPH0716419B2 (ja) 1995-03-01
AU7945682A (en) 1982-07-22
JPH07170978A (ja) 1995-07-11
FI84080C (sv) 1991-10-10
DK15182A (da) 1982-07-17
JPH07108221B2 (ja) 1995-11-22
ES8700320A1 (es) 1986-10-01
ES508813A0 (es) 1983-12-01
GB2091271A (en) 1982-07-28
GB2091271B (en) 1984-08-30
FI820075L (fi) 1982-07-17
JPS57141287A (en) 1982-09-01
DE3280479T2 (de) 1999-03-11
US4666847A (en) 1987-05-19
EP0057350B1 (en) 1998-10-21
ES8401132A1 (es) 1983-12-01
CA1341300C (en) 2001-10-09
NO820090L (no) 1982-07-19
ES524986A0 (es) 1986-10-01
ATE172493T1 (de) 1998-11-15

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