FI96966B - Expressionskasett för produktion av en hirudinvariant i jäst, en plasmid innehållande denna, en jästcell transformerad med denna och ett förfarande för framställning av en hirudinvariant med hjälp av jästcellen - Google Patents
Expressionskasett för produktion av en hirudinvariant i jäst, en plasmid innehållande denna, en jästcell transformerad med denna och ett förfarande för framställning av en hirudinvariant med hjälp av jästcellen Download PDFInfo
- Publication number
- FI96966B FI96966B FI873068A FI873068A FI96966B FI 96966 B FI96966 B FI 96966B FI 873068 A FI873068 A FI 873068A FI 873068 A FI873068 A FI 873068A FI 96966 B FI96966 B FI 96966B
- Authority
- FI
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- Prior art keywords
- yeast
- hirudin
- gene
- plasmid
- sequence
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Botany (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Claims (5)
1. Expressionskasett för produktion av en hirudin-variant i jäst, kännetecknat därav, att den 5 innehäller a) ett första DNA-fragment som kodar för en prekur-sor av hirudinvarianten HV1, vilken prekursor omfattar nämnda hirudinvariant, säsom den i figur 10 angivna hirudinvarianten, och ett extra omräde omfattande mera än tvä 10 aminosyror, vilket har bundits vid hirudinvariantens N- terminal medelst en peptidbindning och vilket innehäller ett enda spjälkningsställe som igenkänns av yscF-proteinas och är dipeptiden Lys-Arg, säsom anges i figur 7, och som bildar det ovan nämnda extra omr&dets C-terminal, 15 b) ett andra DNA-fragment, vilket kodar för en lea- der-peptid, som är pre-pro - eller profragmentet av a-fak-torns pre-pro -prekursor, och vilket andra DNA-fragment bundits vid det första DNA-fragmentets 5-terminal i rätt läsram, och 20 c) ändamälsenliga kontrollomräden för expression av nämnda DNA-fragment, säsom PGK-genpromotorn.
2. Jästcell, kännetecknad därav, att den transformerats med en expressionskasett enligt patentkrav 1. • 25
3. Jästcell enligt patentkrav 2, känne tecknad därav, att expressionskasetten insätts i en plasmid som innehäller en i jäst fungerande replikations-origin.
4. Plasmid, kännetecknad därav, att 30 den innehäller en expressionskasett enligt patentkrav 1 och en i jäst fungerande replikationsorigin.
5. Förfarande för framställning av en hirudinvariant, kännetecknat därav, att en jäst enligt patentkrav 2 eller 3 odlas och hirudinvarianten tas till- 35 vara frän odlingssupernatanten.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR868610090A FR2601383B2 (fr) | 1985-05-02 | 1986-07-10 | Vecteurs perfectionnes d'expression de l'hirudine dans les levures, procede et produit obtenu |
FR8610090 | 1986-07-10 | ||
FR8616722 | 1986-10-01 | ||
FR868616722A FR2607516B2 (fr) | 1986-12-01 | 1986-12-01 | Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu |
Publications (4)
Publication Number | Publication Date |
---|---|
FI873068A0 FI873068A0 (sv) | 1987-07-10 |
FI873068A FI873068A (sv) | 1988-01-11 |
FI96966B true FI96966B (sv) | 1996-06-14 |
FI96966C FI96966C (sv) | 1996-09-25 |
Family
ID=26225387
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI873068A FI96966C (sv) | 1986-07-10 | 1987-07-10 | Expressionskasett för produktion av en hirudinvariant i jäst, en plasmid innehållande denna, en jästcell transformerad med denna och ett förfarande för framställning av en hirudinvariant med hjälp av jästcellen |
Country Status (20)
Country | Link |
---|---|
US (2) | US5591640A (sv) |
EP (1) | EP0252854B1 (sv) |
JP (1) | JP2733602B2 (sv) |
KR (1) | KR960013464B1 (sv) |
AT (1) | ATE153066T1 (sv) |
AU (1) | AU614933B2 (sv) |
BG (1) | BG80504A (sv) |
CA (1) | CA1341490C (sv) |
CZ (1) | CZ278268B6 (sv) |
DE (1) | DE3752063T2 (sv) |
DK (1) | DK175195B1 (sv) |
ES (1) | ES2103700T3 (sv) |
FI (1) | FI96966C (sv) |
GR (1) | GR3024368T3 (sv) |
HU (1) | HU214236B (sv) |
MC (1) | MC1834A1 (sv) |
NO (1) | NO179489C (sv) |
PL (1) | PL154118B1 (sv) |
PT (1) | PT85296B (sv) |
RU (1) | RU1776276C (sv) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
GB8530631D0 (en) | 1985-12-12 | 1986-01-22 | Ciba Geigy Ag | Thrombin inhibitors |
MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
US5256559A (en) * | 1988-03-04 | 1993-10-26 | Biogen, Inc. | Methods and compositions for inhibiting platelet aggregation |
GB8809860D0 (en) * | 1988-04-26 | 1988-06-02 | Ciba Geigy Ag | Process for production of polypeptides |
US5112615A (en) * | 1988-08-03 | 1992-05-12 | New England Deaconess Hospital Corporation | Soluble hirudin conjugates |
US5167960A (en) * | 1988-08-03 | 1992-12-01 | New England Deaconess Hospital Corporation | Hirudin-coated biocompatible substance |
DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
FR2645174B1 (fr) * | 1989-03-31 | 1994-01-07 | Transgene Sa | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant |
US5879926A (en) * | 1989-03-31 | 1999-03-09 | Transgene S.A. | Yeast strains for the production of mature heterologous proteins, especially hirudin |
FR2645175B1 (fr) * | 1989-03-31 | 1994-02-18 | Transgene Sa | Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche |
FR2646437B1 (fr) * | 1989-04-28 | 1991-08-30 | Transgene Sa | Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant |
US5179196A (en) * | 1989-05-04 | 1993-01-12 | Sri International | Purification of proteins employing ctap-iii fusions |
DE4009268A1 (de) * | 1990-03-22 | 1991-09-26 | Consortium Elektrochem Ind | Sekretion von hirudinderivaten |
GB9015825D0 (en) * | 1990-07-18 | 1990-09-05 | Ciba Geigy Ag | In vitro processing of fusion proteins |
US6514730B1 (en) | 1991-03-21 | 2003-02-04 | Consortium für elektrochemische Industrie GmbH | Secretion of hirudin derivatives |
US5837808A (en) * | 1991-08-20 | 1998-11-17 | Baxter International Inc. | Analogs of hirudin |
EP0592358B1 (en) * | 1992-09-04 | 2000-10-11 | Novartis AG | Process for the production of protease inhibitors |
TW282490B (sv) * | 1992-12-15 | 1996-08-01 | Ciba Geigy Ag | |
US5712114A (en) * | 1995-06-06 | 1998-01-27 | Basf Aktiengesellschaft | Compositions for expression of proteins in host cells using a preprocollagen signal sequence |
EP1049790A1 (en) | 1998-01-23 | 2000-11-08 | Novo Nordisk A/S | Process for making desired polypeptides in yeast |
KR100949722B1 (ko) | 2002-08-12 | 2010-03-25 | 에이펙셀 (주) | 마이크로 초미립 분쇄기 |
US7795205B2 (en) | 2004-04-12 | 2010-09-14 | Canyon Pharmaceuticals, Inc. | Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4356270A (en) * | 1977-11-08 | 1982-10-26 | Genentech, Inc. | Recombinant DNA cloning vehicle |
US4769326A (en) * | 1980-02-29 | 1988-09-06 | The Regents Of The University Of California | Expression linkers |
US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
NO840200L (no) * | 1983-01-28 | 1984-07-30 | Cefus Corp | Glukoamylase cdna. |
JPS6041487A (ja) * | 1983-04-25 | 1985-03-05 | ジエネンテツク・インコ−ポレイテツド | 酵母発現系でのアルフア因子配列の使用 |
ATE78294T1 (de) * | 1984-03-27 | 1992-08-15 | Transgene Sa | Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin. |
DE3583361D1 (de) * | 1984-06-14 | 1991-08-08 | Ucp Gen Pharma Ag | Verfahren zur herstellung von thrombin-inhibitoren. |
DE3429430A1 (de) * | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens |
DE3445517C2 (de) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins |
FR2593518B1 (fr) * | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
-
1987
- 1987-07-02 MC MC871896A patent/MC1834A1/xx unknown
- 1987-07-08 AU AU75366/87A patent/AU614933B2/en not_active Expired
- 1987-07-09 CA CA000541712A patent/CA1341490C/fr not_active Expired - Fee Related
- 1987-07-09 RU SU874202962A patent/RU1776276C/ru active
- 1987-07-09 PT PT85296A patent/PT85296B/pt not_active IP Right Cessation
- 1987-07-09 BG BG080504A patent/BG80504A/bg unknown
- 1987-07-09 DK DK198703558A patent/DK175195B1/da not_active IP Right Cessation
- 1987-07-10 NO NO872878A patent/NO179489C/no not_active IP Right Cessation
- 1987-07-10 HU HU873161A patent/HU214236B/hu unknown
- 1987-07-10 PL PL1987266756A patent/PL154118B1/pl unknown
- 1987-07-10 CZ CS875277A patent/CZ278268B6/cs not_active IP Right Cessation
- 1987-07-10 ES ES87401649T patent/ES2103700T3/es not_active Expired - Lifetime
- 1987-07-10 AT AT87401649T patent/ATE153066T1/de not_active IP Right Cessation
- 1987-07-10 EP EP87401649A patent/EP0252854B1/fr not_active Expired - Lifetime
- 1987-07-10 JP JP62173724A patent/JP2733602B2/ja not_active Expired - Lifetime
- 1987-07-10 FI FI873068A patent/FI96966C/sv not_active IP Right Cessation
- 1987-07-10 DE DE3752063T patent/DE3752063T2/de not_active Expired - Lifetime
- 1987-07-10 KR KR1019870007450A patent/KR960013464B1/ko not_active IP Right Cessation
-
1995
- 1995-03-16 US US08/405,765 patent/US5591640A/en not_active Expired - Lifetime
-
1996
- 1996-09-05 US US08/708,543 patent/US5902735A/en not_active Expired - Fee Related
-
1997
- 1997-08-06 GR GR970402015T patent/GR3024368T3/el unknown
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Owner name: BEHRINGWERKE AKTIENGESELLSCHAFT |
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Owner name: SCHERING AKTIENGESELLSCHAFT |
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MA | Patent expired |