FI80597B - Foerfarande foer framstaellning av en plasminogenaktivator. - Google Patents
Foerfarande foer framstaellning av en plasminogenaktivator. Download PDFInfo
- Publication number
- FI80597B FI80597B FI842627A FI842627A FI80597B FI 80597 B FI80597 B FI 80597B FI 842627 A FI842627 A FI 842627A FI 842627 A FI842627 A FI 842627A FI 80597 B FI80597 B FI 80597B
- Authority
- FI
- Finland
- Prior art keywords
- activator
- plasminogen activator
- arginine
- urokinase
- column
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (2)
1. Process för att bereda en plasminogenaktivator här-stammande frän männinskonjurar och besittande följande karakteristiska egenskaper: 5 (1) Huvudproteinbandet vid elektrofores i natriumdodecyl- sulfat-polyakrylamidgel har en skenbar molekylvikt om ca 70,000 ± 5,000; (2) Huvudbandet vid elektrofores till isoelektriska punkten har ett pi i omrädet 7-9; 10 (3) Plasminogenaktivatorn har den immunologiska egenskapen att icke adsorberas vid affinitetskromatografi med anti-urokinas-IgG-Sepharose; (4) Plasminogenaktivatorn hydrolyserar H-D-valyl-L-leucyl-L-lysin-p-nitroaniliddihydroklorid och H-D-isoleucyl-L-15 prolyl-L-arginin-p-nitroaniliddihydroklorid; men hydrolyserar icke Boc-L-valyl-L-prolyl-L-arginin-4-metylkumaryl- 7-amid, karbobensoxyl-L-fenylalanyl-L-arginin-4-metyl-kumaryl-7-amid, L-prolyl-L-fenylalanyl-L-arginin-4-metyl-kumaryl-7-amid och glutamyl-glycyl-L-arginin-4-metylkumaryl-20 7-amid; vilken process är kännetecknad av att männinskonjure extraheras med ammoniumtiocyanatlösning; varefter det ernädda extraktet fär passera en kolonnvald ur följande grupp av kolonner: en kolonn packad med ett 25 jonbytarmaterial, en kolonn med ett metalkelat, en kolonn packad med L-arginin eller ett argininderivat bundet vid en bärare, en kolonn packad med ett hemagglutinin bundet vid en bärare och en kolonn packad med en bärare med molekylsikts egenskaper eller en kombination av kolonner ur nämnda grupp, 30 i avsikt att rena detta.
2. Process enligt krav 1, kännetecknad av att männinskonjuren extraheras med en ammoniumtiocynat-lösning som buffrats med Tris-HCl i pH-omrädet 7.0 - 7.4.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18906582 | 1982-10-29 | ||
| JP57189065A JPH0637398B2 (ja) | 1982-10-29 | 1982-10-29 | 血栓溶解剤 |
| JP18906782 | 1982-10-29 | ||
| JP57189067A JPS5980613A (ja) | 1982-10-29 | 1982-10-29 | 新規なプラスミノ−ゲン・アクチベ−タ及びその製造方法 |
| JP8300386 | 1983-10-27 | ||
| PCT/JP1983/000386 WO1984001714A1 (en) | 1982-10-29 | 1983-10-27 | Novel plasminogen activator, process for its preparation, and thrombolytic drug containing the same |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI842627A0 FI842627A0 (fi) | 1984-06-29 |
| FI842627A FI842627A (fi) | 1984-06-29 |
| FI80597B true FI80597B (fi) | 1990-03-30 |
| FI80597C FI80597C (sv) | 1990-07-10 |
Family
ID=26505289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI842627A FI80597C (sv) | 1982-10-29 | 1984-06-29 | Förfarande för framställning av en plasminogenaktivator |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0124613B1 (sv) |
| CA (1) | CA1221029A (sv) |
| CH (1) | CH660742A5 (sv) |
| DE (1) | DE3390272C2 (sv) |
| DK (1) | DK169686B1 (sv) |
| FI (1) | FI80597C (sv) |
| GB (1) | GB2138824B (sv) |
| IT (1) | IT1169910B (sv) |
| NL (1) | NL191755C (sv) |
| NO (1) | NO166314C (sv) |
| SE (1) | SE8403375D0 (sv) |
| WO (1) | WO1984001714A1 (sv) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3582338D1 (de) * | 1984-01-30 | 1991-05-08 | Asahi Chemical Ind | Verfahren zur herstellung eines doppelkettigen plasminogenaktivators. |
| US4929444A (en) * | 1985-05-28 | 1990-05-29 | Burroughs Wellcome Co. | Low pH pharmaceutical formulation containing t-PA |
| IL78937A (en) * | 1985-05-28 | 1990-11-29 | Wellcome Found | Pharmaceutical compositions containing tissue-plasminogen activator |
| EP0275282A1 (en) * | 1986-07-16 | 1988-07-27 | Celltech Limited | Process for purifying a plasminogen activator |
| JPS6379591A (ja) * | 1986-09-22 | 1988-04-09 | Mitsui Toatsu Chem Inc | ｔＰＡの精製方法 |
| AU1668988A (en) * | 1987-06-03 | 1988-12-08 | Smithkline Beckman Corporation | Purification of tpa |
| DE3726019A1 (de) * | 1987-08-05 | 1989-02-16 | Boehringer Mannheim Gmbh | Verfahren zur abtrennung und reinigung von t-pa |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8003402A (nl) * | 1980-06-11 | 1982-01-04 | Leuven Res & Dev Vzw | Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking. |
| IL68561A (en) * | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
| JPS5913732A (ja) * | 1982-07-16 | 1984-01-24 | Mitsui Toatsu Chem Inc | 血栓溶解剤 |
| IE81073B1 (en) * | 1986-03-18 | 2000-01-12 | Genentech Inc | Modified human tissue-type plasminogen activator and its preparation |
-
1983
- 1983-10-27 WO PCT/JP1983/000386 patent/WO1984001714A1/ja active IP Right Grant
- 1983-10-27 GB GB08414992A patent/GB2138824B/en not_active Expired
- 1983-10-27 NL NL8320346A patent/NL191755C/xx not_active IP Right Cessation
- 1983-10-27 EP EP83903315A patent/EP0124613B1/en not_active Expired - Lifetime
- 1983-10-27 DE DE19833390272 patent/DE3390272C2/de not_active Expired
- 1983-10-27 CH CH3063/84A patent/CH660742A5/de not_active IP Right Cessation
- 1983-10-28 IT IT23533/83A patent/IT1169910B/it active
- 1983-10-28 CA CA000439941A patent/CA1221029A/en not_active Expired
-
1984
- 1984-06-19 NO NO84842468A patent/NO166314C/no unknown
- 1984-06-22 DK DK307384A patent/DK169686B1/da active
- 1984-06-25 SE SE8403375A patent/SE8403375D0/sv unknown
- 1984-06-29 FI FI842627A patent/FI80597C/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO166314B (no) | 1991-03-25 |
| GB2138824B (en) | 1986-12-31 |
| DK307384A (da) | 1984-06-22 |
| EP0124613A1 (en) | 1984-11-14 |
| EP0124613B1 (en) | 1992-12-30 |
| EP0124613A4 (en) | 1986-07-29 |
| NL8320346A (nl) | 1984-09-03 |
| DK169686B1 (da) | 1995-01-09 |
| IT1169910B (it) | 1987-06-03 |
| CH660742A5 (de) | 1987-06-15 |
| DK307384D0 (da) | 1984-06-22 |
| NO166314C (no) | 1991-07-03 |
| NL191755C (nl) | 1996-07-02 |
| GB2138824A (en) | 1984-10-31 |
| FI842627A0 (fi) | 1984-06-29 |
| CA1221029A (en) | 1987-04-28 |
| FI80597C (sv) | 1990-07-10 |
| DE3390272C2 (de) | 1987-09-17 |
| SE8403375L (sv) | 1984-06-25 |
| IT8323533A0 (it) | 1983-10-28 |
| NO842468L (no) | 1984-06-19 |
| DE3390272T1 (de) | 1984-11-29 |
| WO1984001714A1 (en) | 1984-05-10 |
| NL191755B (nl) | 1996-03-01 |
| GB8414992D0 (en) | 1984-07-18 |
| FI842627A (fi) | 1984-06-29 |
| SE8403375D0 (sv) | 1984-06-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC | Transfer of assignment of patent |
Owner name: MITSUI CHEMICALS, INC. |
|
| MM | Patent lapsed |
Owner name: SCHERING AKTIENGESELLSCHAFT |