FI57783C - Foerfarande foer bestaemning av kolesterol - Google Patents
Foerfarande foer bestaemning av kolesterol Download PDFInfo
- Publication number
- FI57783C FI57783C FI941/74A FI94174A FI57783C FI 57783 C FI57783 C FI 57783C FI 941/74 A FI941/74 A FI 941/74A FI 94174 A FI94174 A FI 94174A FI 57783 C FI57783 C FI 57783C
- Authority
- FI
- Finland
- Prior art keywords
- cholesterol
- spec
- streptomyces
- candida
- determining
- Prior art date
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 146
- 235000012000 cholesterol Nutrition 0.000 claims description 73
- 108010055297 Sterol Esterase Proteins 0.000 claims description 49
- 102000000019 Sterol Esterase Human genes 0.000 claims description 49
- 238000000034 method Methods 0.000 claims description 35
- 244000005700 microbiome Species 0.000 claims description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims description 21
- 108010089254 Cholesterol oxidase Proteins 0.000 claims description 19
- 150000001840 cholesterol esters Chemical class 0.000 claims description 16
- 241000222122 Candida albicans Species 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 12
- 229940095731 candida albicans Drugs 0.000 claims description 12
- 241000222175 Diutina rugosa Species 0.000 claims description 11
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 claims description 10
- 241000187747 Streptomyces Species 0.000 claims description 9
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 7
- 239000004094 surface-active agent Substances 0.000 claims description 7
- 241000228212 Aspergillus Species 0.000 claims description 6
- 102000016938 Catalase Human genes 0.000 claims description 6
- 108010053835 Catalase Proteins 0.000 claims description 6
- 241000235527 Rhizopus Species 0.000 claims description 6
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 claims description 6
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 claims description 6
- 241000186046 Actinomyces Species 0.000 claims description 5
- 102000003992 Peroxidases Human genes 0.000 claims description 5
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 4
- 241000936713 Streptomyces griseomycini Species 0.000 claims description 4
- 241000187179 Streptomyces tendae Species 0.000 claims description 4
- 241000589220 Acetobacter Species 0.000 claims description 3
- 241000228143 Penicillium Species 0.000 claims description 3
- 241000204087 Pseudonocardia autotrophica Species 0.000 claims description 3
- 150000002429 hydrazines Chemical class 0.000 claims description 3
- 125000000468 ketone group Chemical group 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims description 3
- 241001149951 Mucor mucedo Species 0.000 claims description 2
- 150000007857 hydrazones Chemical class 0.000 claims description 2
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 14
- 230000000694 effects Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 239000000843 powder Substances 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000007127 saponification reaction Methods 0.000 description 6
- 241000187654 Nocardia Species 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 5
- 238000008620 Cholesterol Assay Methods 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 150000001450 anions Chemical class 0.000 description 4
- 230000008033 biological extinction Effects 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 150000002148 esters Chemical class 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000008057 potassium phosphate buffer Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- HORQAOAYAYGIBM-UHFFFAOYSA-N 2,4-dinitrophenylhydrazine Chemical compound NNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O HORQAOAYAYGIBM-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000007824 enzymatic assay Methods 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- RQNOFUZGXHSHOT-UHFFFAOYSA-N 1-(diethylamino)ethanol Chemical group CCN(CC)C(C)O RQNOFUZGXHSHOT-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229930182558 Sterol Natural products 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000001166 ammonium sulphate Substances 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 108010023417 cholesterol dehydrogenase Proteins 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 108010085346 steroid delta-isomerase Proteins 0.000 description 2
- 150000003432 sterols Chemical class 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000228257 Aspergillus sp. Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000186311 Brevibacterium sterolicum Species 0.000 description 1
- 241000235556 Cunninghamella elegans Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241001251094 Formica Species 0.000 description 1
- 229930194542 Keto Natural products 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- RJECHNNFRHZQKU-UHFFFAOYSA-N Oelsaeurecholesterylester Natural products C12CCC3(C)C(C(C)CCCC(C)C)CCC3C2CC=C2C1(C)CCC(OC(=O)CCCCCCCC=CCCCCCCCC)C2 RJECHNNFRHZQKU-UHFFFAOYSA-N 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000187563 Rhodococcus ruber Species 0.000 description 1
- 241000520747 Streptomyces canescens Species 0.000 description 1
- 241000187130 Streptomyces chartreusis Species 0.000 description 1
- 241000936751 Streptomyces cyaneofuscatus Species 0.000 description 1
- 241000936729 Streptomyces hachijoensis Species 0.000 description 1
- 241000946855 Streptomyces malachiticus Species 0.000 description 1
- 241000946864 Streptomyces michiganensis Species 0.000 description 1
- 241001468239 Streptomyces murinus Species 0.000 description 1
- 241000946794 Streptomyces roseolus Species 0.000 description 1
- 241000946767 Streptomyces toxytricini Species 0.000 description 1
- 241000946738 Streptomyces variabilis Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- RJECHNNFRHZQKU-RMUVNZEASA-N cholesteryl oleate Chemical compound C([C@@H]12)C[C@]3(C)[C@@H]([C@H](C)CCCC(C)C)CC[C@H]3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)CCCCCCC\C=C/CCCCCCCC)C1 RJECHNNFRHZQKU-RMUVNZEASA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 150000002632 lipids Chemical group 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/827—Actinoplanes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/893—Streptomyces chartreusis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/913—Aspergillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/921—Candida
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/921—Candida
- Y10S435/922—Candida albicans
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/933—Penicillium
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2315501 | 1973-03-28 | ||
| DE2315501A DE2315501C3 (de) | 1973-03-28 | 1973-03-28 | Verfahren zur Bestimmung von Cholesterin |
| DE2316637A DE2316637A1 (de) | 1973-03-28 | 1973-04-03 | Verfahren zur bestimmung von cholesterin |
| DE2316637 | 1973-04-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FI57783B FI57783B (fi) | 1980-06-30 |
| FI57783C true FI57783C (fi) | 1980-10-10 |
Family
ID=25764885
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI941/74A FI57783C (fi) | 1973-03-28 | 1974-03-27 | Foerfarande foer bestaemning av kolesterol |
Country Status (20)
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3898130A (en) * | 1974-03-18 | 1975-08-05 | American Hospital Supply Corp | Rapid enzymatic hydrolysis of triglycerides |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
| GB1515195A (en) * | 1975-08-05 | 1978-06-21 | Hycel Inc | Triglyceride assay |
| US4184921A (en) * | 1976-03-25 | 1980-01-22 | Boehringer Mannheim Gmbh | Process and reagent for determining cholesterol |
| CA1114269A (en) * | 1976-08-19 | 1981-12-15 | Charles D. Warburton | Integral element for the detection of glycerol or triglycerides |
| CA1100023A (en) * | 1976-08-19 | 1981-04-28 | Charles T. Goodhue | Process and composition for the quantification of glycerol and triglycerides |
| US4042461A (en) | 1976-09-10 | 1977-08-16 | Eastman Kodak Company | Method for purifying cholesterol esterase |
| CA1104041A (en) * | 1977-02-03 | 1981-06-30 | Charles T. Goodhue | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4259440A (en) * | 1979-05-21 | 1981-03-31 | Miles Laboratories, Inc. | Hydrolysis and assay of triglycerides |
| DE2933648A1 (de) * | 1979-08-20 | 1981-03-26 | Boehringer Mannheim Gmbh, 68305 Mannheim | Verfahren zur gewinnung von cholesterinesterase |
| DE2933646A1 (de) * | 1979-08-20 | 1981-03-26 | Boehringer Mannheim Gmbh, 68305 Mannheim | Verfahren zur gewinnung von cholesterinesterase |
| DE3065827D1 (en) * | 1979-08-23 | 1984-01-12 | Ivan Endre Modrovich | Method of stabilizing an enzyme solution for use in total cholesterol determination, stabilized solution and test kit therefor |
| US4378429A (en) * | 1979-08-23 | 1983-03-29 | Modrovich Ivan Endre | Enzymatic method and stabilized solutions for determining total cholesterol in human serum |
| US4425427A (en) | 1980-03-13 | 1984-01-10 | Vitafin N.V. | Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components |
| IT1132230B (it) * | 1980-07-24 | 1986-06-25 | Anic Spa | Procedimento per la produzione dell'enzima colesterolo-esterasi e per idrolisi degli esteri con acidi grassi del colesterolo mediante l'impiego dell'enzima stesso |
| US5456932A (en) * | 1987-04-20 | 1995-10-10 | Fuisz Technologies Ltd. | Method of converting a feedstock to a shearform product and product thereof |
| US5387431A (en) * | 1991-10-25 | 1995-02-07 | Fuisz Technologies Ltd. | Saccharide-based matrix |
| EP0395654A4 (en) * | 1987-09-22 | 1991-01-09 | Abbott Laboratories | Simultaneous assay for cholesterol and triglycerides |
| US5156954A (en) * | 1989-03-08 | 1992-10-20 | Cholestech Corporation | Assay device and method using a signal-modulating compound |
| US5171688A (en) * | 1988-08-30 | 1992-12-15 | Cholestech Corporation | Self-corrected assay device |
| JP2514087B2 (ja) * | 1989-01-06 | 1996-07-10 | 富士写真フイルム株式会社 | 総コレステロ―ル分析要素 |
| US5114350A (en) * | 1989-03-08 | 1992-05-19 | Cholestech Corporation | Controlled-volume assay apparatus |
| US5340539A (en) * | 1989-03-16 | 1994-08-23 | Chemtrak, Inc. | Non-instrumented cholesterol assay |
| US5110724A (en) * | 1990-04-02 | 1992-05-05 | Cholestech Corporation | Multi-analyte assay device |
| DE69232721T2 (de) * | 1991-05-17 | 2002-12-05 | Biovail Technologies Ltd., Chantilly | Enzymsysteme |
| US5576042A (en) * | 1991-10-25 | 1996-11-19 | Fuisz Technologies Ltd. | High intensity particulate polysaccharide based liquids |
| CA2129117C (en) * | 1992-01-31 | 1999-01-05 | Mark D. White | Inhibition of catalase activity in biological fluids |
| US5346377A (en) * | 1993-10-07 | 1994-09-13 | Fuisz Technologies Ltd. | Apparatus for flash flow processing having feed rate control |
| US5445769A (en) * | 1994-06-27 | 1995-08-29 | Fuisz Technologies Ltd. | Spinner head for flash flow processing |
| US5582855A (en) * | 1994-07-01 | 1996-12-10 | Fuisz Technologies Ltd. | Flash flow formed solloid delivery systems |
| US5556652A (en) * | 1994-08-05 | 1996-09-17 | Fuisz Technologies Ltd. | Comestibles containing stabilized highly odorous flavor component delivery systems |
| US5587198A (en) * | 1995-05-31 | 1996-12-24 | Fuisz Technologies Ltd. | Positive hydration method of preparing confectionery and product therefrom |
| DE60207196T2 (de) | 2002-04-09 | 2006-07-20 | Cholestech Corp., Hayward | Verfahren und Vorrichtung zur Quantifizierung von Lipoprotein-Cholesterol hoher Dichte |
| US20050074828A1 (en) * | 2003-07-16 | 2005-04-07 | Dimagno Theodore John | Uae of lipase for high-density lipoprotein cholesterol detection |
| JP4643212B2 (ja) * | 2003-11-28 | 2011-03-02 | シスメックス株式会社 | コレステロール脱水素酵素の安定化方法、コレステロール脱水素酵素含有組成物及びコレステロール測定試薬 |
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| US9828624B2 (en) | 2013-07-24 | 2017-11-28 | Boston Heart Diagnostics Corporation | Driving patient compliance with therapy |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3607093A (en) * | 1968-02-15 | 1971-09-21 | Schering Corp | Devices for testing biological liquids |
| FR2047147A5 (enrdf_load_stackoverflow) * | 1969-05-06 | 1971-03-12 | Kyowa Hakko Kogyo Kk | |
| AR195000A1 (es) * | 1972-05-17 | 1973-08-30 | Boehringer Mannheim Gmbh | Procedimiento para la determinacion de colesterol |
| DK158602C (da) * | 1983-05-11 | 1990-11-19 | Interholz Technik Gmbh | Fremgangsmaade og apparat til at rette langstrakte traeelementer op |
-
1973
- 1973-03-28 DE DE2315501A patent/DE2315501C3/de not_active Expired
- 1973-04-03 DE DE2316637A patent/DE2316637A1/de active Pending
-
1974
- 1974-03-13 IT IT7467220Q patent/IT1024524B/it active
- 1974-03-24 IL IL44480A patent/IL44480A/en unknown
- 1974-03-25 US US454522A patent/US3925164A/en not_active Expired - Lifetime
- 1974-03-25 AR AR252946A patent/AR200596A1/es active
- 1974-03-25 NL NL7403978.A patent/NL157111B/xx not_active IP Right Cessation
- 1974-03-25 GB GB1315974A patent/GB1429526A/en not_active Expired
- 1974-03-25 CH CH413574A patent/CH594887A5/xx not_active IP Right Cessation
- 1974-03-26 BE BE142475A patent/BE812858A/xx not_active IP Right Cessation
- 1974-03-26 DD DD177462A patent/DD110356A5/xx unknown
- 1974-03-27 HU HU74BO1493A patent/HU174541B/hu unknown
- 1974-03-27 SE SE7404115A patent/SE460057B/xx not_active IP Right Cessation
- 1974-03-27 FI FI941/74A patent/FI57783C/fi active
- 1974-03-27 CA CA196,179A patent/CA1067804A/en not_active Expired
- 1974-03-28 YU YU00860/74A patent/YU86074A/xx unknown
- 1974-03-28 DK DK172374A patent/DK151396C/da not_active IP Right Cessation
- 1974-03-28 FR FR7410953A patent/FR2223696B1/fr not_active Expired
-
1979
- 1979-06-15 KE KE2968A patent/KE2968A/xx unknown
- 1979-07-12 HK HK466/79A patent/HK46679A/xx unknown
-
1980
- 1980-12-30 MY MY101/80A patent/MY8000101A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2316637A1 (de) | 1974-10-17 |
| IL44480A0 (en) | 1974-06-30 |
| IL44480A (en) | 1976-12-31 |
| YU86074A (en) | 1984-04-30 |
| FR2223696A1 (enrdf_load_stackoverflow) | 1974-10-25 |
| GB1429526A (en) | 1976-03-24 |
| CA1067804A (en) | 1979-12-11 |
| DK151396C (da) | 1988-07-04 |
| MY8000101A (en) | 1980-12-31 |
| BE812858A (fr) | 1974-09-26 |
| DE2315501A1 (de) | 1974-10-17 |
| FI57783B (fi) | 1980-06-30 |
| IT1024524B (it) | 1978-07-20 |
| DK151396B (da) | 1987-11-30 |
| SE460057B (sv) | 1989-09-04 |
| FR2223696B1 (enrdf_load_stackoverflow) | 1976-10-08 |
| US3925164A (en) | 1975-12-09 |
| AU6730074A (en) | 1975-10-02 |
| DE2315501C3 (de) | 1980-02-21 |
| DE2315501B2 (de) | 1979-04-26 |
| AR200596A1 (es) | 1974-11-22 |
| HU174541B (hu) | 1980-02-28 |
| CH594887A5 (enrdf_load_stackoverflow) | 1978-01-31 |
| KE2968A (en) | 1979-07-20 |
| NL7403978A (enrdf_load_stackoverflow) | 1974-10-01 |
| NL157111B (nl) | 1978-06-15 |
| DD110356A5 (enrdf_load_stackoverflow) | 1974-12-12 |
| HK46679A (en) | 1979-07-20 |
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