FI20115310L - Proteaasientsyymi ja sen käytöt - Google Patents
Proteaasientsyymi ja sen käytötInfo
- Publication number
- FI20115310L FI20115310L FI20115310A FI20115310A FI20115310L FI 20115310 L FI20115310 L FI 20115310L FI 20115310 A FI20115310 A FI 20115310A FI 20115310 A FI20115310 A FI 20115310A FI 20115310 L FI20115310 L FI 20115310L
- Authority
- FI
- Finland
- Prior art keywords
- enzyme
- protease
- serine protease
- amino acid
- acid sequence
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 4
- 102000012479 Serine Proteases Human genes 0.000 abstract 4
- 108010022999 Serine Proteases Proteins 0.000 abstract 4
- 239000004365 Protease Substances 0.000 abstract 3
- 150000001413 amino acids Chemical group 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 230000002538 fungal effect Effects 0.000 abstract 2
- 210000004209 hair Anatomy 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 241000228423 Malbranchea Species 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 239000010985 leather Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 210000002268 wool Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2065—Polyhydric alcohols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/12—Soft surfaces, e.g. textile
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/14—Hard surfaces
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Emergency Medicine (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Esillä oleva keksintö kohdistuu sieniperäiseen seriiniproteaasientsyymiin, jolla mainitulla entsyymillä on seriiniproteaasin aktiivisuutta ja jolla on kypsän Malbranchea ALKO4122-proteaasin aminohapposekvenssi, joka on määritelty sekvenssissä SEQ ID NO:18, tai aminohapposekvenssi, jonka samanlaisuus sekvenssin SEQ ID NO:18 mukaisen aminohapposekvenssin kanssa on vähintään 66 %. Samoin esitetään eristetty nukleiinihappomolekyyli, jonka sisältämä polynukleotidisekvenssi koodaa sieniperäistä seriiniproteaasientsyymiä, mainittua proteaasia koodaavat nukleiinihapposekvenssit, isäntäsolu ja menetelmä polypeptidin, jolla on seriiniproteaasin aktiivisuutta, tuottamiseksi. Mainittu proteaasi on käyttökelpoinen entsyymivalmisteena, jota voidaan käyttää pesuainekoostumuksissa ja kuitujen, villan, hiusten/karvojen, nahan ja silkin käsittelyyn, elintarvikkeiden ja eläinravinnon käsittelyyn tai mihin tahansa muihin sellaisiin sovelluksiin, joihin liittyy proteiinipitoisen materiaalin muokkausta, hajotusta tai poistoa.
Priority Applications (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI20115310A FI123425B (fi) | 2011-03-31 | 2011-03-31 | Proteaasientsyymi ja sen käytöt |
US13/433,984 US9404164B2 (en) | 2011-03-31 | 2012-03-29 | Protease enzyme and uses thereof |
JP2014501649A JP2014511681A (ja) | 2011-03-31 | 2012-03-30 | プロテアーゼ酵素およびその使用 |
CN201280026496.9A CN103890172B (zh) | 2011-03-31 | 2012-03-30 | 蛋白酶及其应用 |
BR112013025238A BR112013025238A2 (pt) | 2011-03-31 | 2012-03-30 | enzima serina protease, molécula de ácido nucleico isolada, vetor de expressão recombinante, célula hospedeira, processos para produzir um polipeptídeo com atividade serina protease, e para obter uma preparação enzimática, polipeptídeo com atividade serina protease, preparação enzimática, uso de enzima serina protease, e, composição de detergente |
ES12722084.6T ES2585652T3 (es) | 2011-03-31 | 2012-03-30 | Enzima proteasa y usos de la misma |
EP12722084.6A EP2691520B1 (en) | 2011-03-31 | 2012-03-30 | Protease enzyme and uses thereof |
KR1020137029011A KR102010747B1 (ko) | 2011-03-31 | 2012-03-30 | 프로테아제 효소 및 이의 용도 |
RU2013146665A RU2611043C2 (ru) | 2011-03-31 | 2012-03-30 | Фермент протеаза и его применения |
DK12722084.6T DK2691520T3 (en) | 2011-03-31 | 2012-03-30 | Protease enzyme and uses thereof |
PCT/EP2012/055762 WO2012131023A2 (en) | 2011-03-31 | 2012-03-30 | Protease enzyme and uses thereof |
US15/196,716 US10221377B2 (en) | 2011-03-31 | 2016-06-29 | Protease enzyme and uses thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI20115310A FI123425B (fi) | 2011-03-31 | 2011-03-31 | Proteaasientsyymi ja sen käytöt |
FI20115310 | 2011-03-31 |
Publications (4)
Publication Number | Publication Date |
---|---|
FI20115310A0 FI20115310A0 (fi) | 2011-03-31 |
FI20115310L true FI20115310L (fi) | 2012-10-01 |
FI20115310A FI20115310A (fi) | 2012-10-01 |
FI123425B FI123425B (fi) | 2013-04-30 |
Family
ID=43806518
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI20115310A FI123425B (fi) | 2011-03-31 | 2011-03-31 | Proteaasientsyymi ja sen käytöt |
Country Status (11)
Country | Link |
---|---|
US (2) | US9404164B2 (fi) |
EP (1) | EP2691520B1 (fi) |
JP (1) | JP2014511681A (fi) |
KR (1) | KR102010747B1 (fi) |
CN (1) | CN103890172B (fi) |
BR (1) | BR112013025238A2 (fi) |
DK (1) | DK2691520T3 (fi) |
ES (1) | ES2585652T3 (fi) |
FI (1) | FI123425B (fi) |
RU (1) | RU2611043C2 (fi) |
WO (1) | WO2012131023A2 (fi) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2712915A1 (en) * | 2012-10-01 | 2014-04-02 | The Procter and Gamble Company | Methods of treating a surface and compositions for use therein |
WO2015158719A1 (en) * | 2014-04-15 | 2015-10-22 | Aalborg Universitet | Composition and method for degradation of keratinaceous materials |
EP3645147A1 (en) | 2017-06-30 | 2020-05-06 | Diversey, Inc. | Membrane cleaning solution and method of accelerated membrane cleaning using the same |
GB2569611B (en) * | 2017-12-21 | 2020-02-19 | Cordina Ltd | Improvements in hair-curling devices |
CN112639066B (zh) * | 2018-08-27 | 2023-08-22 | 巴斯夫欧洲公司 | 改进去污力的聚合物活性成分 |
EP3636735B1 (en) * | 2018-10-12 | 2024-03-27 | AB Enzymes Oy | Protease enzyme variants and uses thereof |
Family Cites Families (49)
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GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
DK563986A (da) | 1986-11-25 | 1988-06-17 | Novo Industri As | Fremstilling af en lav-temperatur-aktiv protease |
DK564086A (da) | 1986-11-25 | 1988-06-17 | Novo Industri As | Enzymatisk detergent-additiv |
JP2731407B2 (ja) | 1987-04-03 | 1998-03-25 | アムジエン・インコーポレーテツド | 新規なプロテアーゼ酵素 |
DK571587D0 (da) | 1987-11-02 | 1987-11-02 | Novo Industri As | Enzymatisk detergentsammensaetning |
ATE129523T1 (de) | 1988-01-07 | 1995-11-15 | Novo Nordisk As | Spezifische protease. |
US5288627A (en) | 1988-01-07 | 1994-02-22 | Novo Nordisk A/S | Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents |
JPH0241398A (ja) | 1988-07-20 | 1990-02-09 | Novo Ind As | 安定化酵素液体洗剤組成物 |
US5089163A (en) * | 1989-01-30 | 1992-02-18 | Lever Brothers Company, Division Of Conopco, Inc. | Enzymatic liquid detergent composition |
DK316989D0 (da) | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
DK204290D0 (da) | 1990-08-24 | 1990-08-24 | Novo Nordisk As | Enzymatisk detergentkomposition og fremgangsmaade til enzymstabilisering |
DK223790D0 (da) | 1990-09-18 | 1990-09-18 | Novo Nordisk As | Proteaseholdig detergentkomposition |
DK73791D0 (da) | 1991-04-22 | 1991-04-22 | Novo Nordisk As | Detergentadditiv |
CA2069147A1 (en) | 1991-05-22 | 1992-11-23 | Kazuaki Kitano | Dna and its use |
US5962765A (en) | 1991-08-08 | 1999-10-05 | Boyce Thompson Institute For Plant Research, Inc. | Molecular cloning of a complimentary DNA sequence encoding a cuticle degrading protease produced by entomopathogenic fungi |
DK52393D0 (fi) | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
JPH08511299A (ja) * | 1994-03-21 | 1996-11-26 | エス.シー.ジョンソン アンド サン,インコーポレーテッド | 安定な酵素含有水性洗濯予備染み抜き組成物 |
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AU695776B2 (en) | 1995-07-12 | 1998-08-20 | Novozymes A/S | Cleaning-in-place with a solution containing a protease and a lipase |
US6008029A (en) | 1995-08-25 | 1999-12-28 | Novo Nordisk Biotech Inc. | Purified coprinus laccases and nucleic acids encoding the same |
AR005601A1 (es) | 1996-01-29 | 1999-06-23 | Novozymes As | Proceso para la remocion o blanqueo de suciedad o manchas presentes en telas celulosicas y proceso para el lavado de telas celulosicas manchadas o sucias y composicion detergente |
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JP5095884B2 (ja) | 1997-08-29 | 2012-12-12 | ノボザイムス アクティーゼルスカブ | プロテアーゼ変異体及び組成物 |
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WO1999064555A1 (en) * | 1998-06-05 | 1999-12-16 | The Procter & Gamble Company | Granular, powder, and tablet detergent compositions containing gasified particulate matter |
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CN1187443C (zh) * | 2003-05-21 | 2005-02-02 | 云南大学 | 真菌碱性丝氨酸蛋白酶及其制备方法和应用 |
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KR20140072201A (ko) | 2006-07-05 | 2014-06-12 | 카탈리스트 바이오사이언시즈, 인코포레이티드 | 프로테아제 스크리닝 방법 및 이에 의해 확인된 프로테아제 |
US10590368B2 (en) | 2007-03-27 | 2020-03-17 | Novozymes A/S | Stable enzyme solutions and method of manufacturing |
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CN101487002A (zh) * | 2009-03-02 | 2009-07-22 | 华南理工大学 | 碱性蛋白酶 |
FI121712B (fi) | 2009-04-30 | 2011-03-15 | Ab Enzymes Oy | Uusi sieniperäinen proteaasi ja sen käyttö |
FI121711B (fi) | 2009-04-30 | 2011-03-15 | Ab Enzymes Oy | Sieniperäinen seriiniproteaasi ja sen käyttö |
FI121851B (fi) | 2009-07-08 | 2011-05-13 | Ab Enzymes Oy | Sieniperäinen proteaasi ja sen käyttö |
FI123942B (fi) | 2010-10-29 | 2013-12-31 | Ab Enzymes Oy | Sieniperäisen seriiniproteaasin variantteja |
-
2011
- 2011-03-31 FI FI20115310A patent/FI123425B/fi active IP Right Grant
-
2012
- 2012-03-29 US US13/433,984 patent/US9404164B2/en active Active
- 2012-03-30 EP EP12722084.6A patent/EP2691520B1/en active Active
- 2012-03-30 KR KR1020137029011A patent/KR102010747B1/ko active IP Right Grant
- 2012-03-30 ES ES12722084.6T patent/ES2585652T3/es active Active
- 2012-03-30 BR BR112013025238A patent/BR112013025238A2/pt not_active Application Discontinuation
- 2012-03-30 RU RU2013146665A patent/RU2611043C2/ru active
- 2012-03-30 JP JP2014501649A patent/JP2014511681A/ja active Pending
- 2012-03-30 CN CN201280026496.9A patent/CN103890172B/zh active Active
- 2012-03-30 WO PCT/EP2012/055762 patent/WO2012131023A2/en active Application Filing
- 2012-03-30 DK DK12722084.6T patent/DK2691520T3/en active
-
2016
- 2016-06-29 US US15/196,716 patent/US10221377B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
KR102010747B1 (ko) | 2019-08-14 |
RU2611043C2 (ru) | 2017-02-20 |
US9404164B2 (en) | 2016-08-02 |
WO2012131023A2 (en) | 2012-10-04 |
US20160376530A1 (en) | 2016-12-29 |
JP2014511681A (ja) | 2014-05-19 |
EP2691520A2 (en) | 2014-02-05 |
DK2691520T3 (en) | 2016-08-22 |
US10221377B2 (en) | 2019-03-05 |
BR112013025238A2 (pt) | 2018-09-25 |
FI123425B (fi) | 2013-04-30 |
KR20140032396A (ko) | 2014-03-14 |
ES2585652T3 (es) | 2016-10-07 |
RU2013146665A (ru) | 2015-05-20 |
CN103890172B (zh) | 2015-11-25 |
US20120252064A1 (en) | 2012-10-04 |
WO2012131023A3 (en) | 2012-11-22 |
FI20115310A (fi) | 2012-10-01 |
CN103890172A (zh) | 2014-06-25 |
FI20115310A0 (fi) | 2011-03-31 |
EP2691520B1 (en) | 2016-05-18 |
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