FI20115310L - Proteaasientsyymi ja sen käytöt - Google Patents

Proteaasientsyymi ja sen käytöt

Info

Publication number
FI20115310L
FI20115310L FI20115310A FI20115310A FI20115310L FI 20115310 L FI20115310 L FI 20115310L FI 20115310 A FI20115310 A FI 20115310A FI 20115310 A FI20115310 A FI 20115310A FI 20115310 L FI20115310 L FI 20115310L
Authority
FI
Finland
Prior art keywords
enzyme
protease
serine protease
amino acid
acid sequence
Prior art date
Application number
FI20115310A
Other languages
English (en)
Swedish (sv)
Other versions
FI123425B (fi
FI20115310A (fi
FI20115310A0 (fi
Inventor
Marja Paloheimo
Pentti Ojapalo
Leena Valtakari
Kari Juntunen
Original Assignee
Ab Enzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ab Enzymes Oy filed Critical Ab Enzymes Oy
Publication of FI20115310A0 publication Critical patent/FI20115310A0/fi
Priority to FI20115310A priority Critical patent/FI123425B/fi
Priority to US13/433,984 priority patent/US9404164B2/en
Priority to EP12722084.6A priority patent/EP2691520B1/en
Priority to DK12722084.6T priority patent/DK2691520T3/en
Priority to BR112013025238A priority patent/BR112013025238A2/pt
Priority to ES12722084.6T priority patent/ES2585652T3/es
Priority to JP2014501649A priority patent/JP2014511681A/ja
Priority to KR1020137029011A priority patent/KR102010747B1/ko
Priority to RU2013146665A priority patent/RU2611043C2/ru
Priority to CN201280026496.9A priority patent/CN103890172B/zh
Priority to PCT/EP2012/055762 priority patent/WO2012131023A2/en
Publication of FI20115310L publication Critical patent/FI20115310L/fi
Publication of FI20115310A publication Critical patent/FI20115310A/fi
Application granted granted Critical
Publication of FI123425B publication Critical patent/FI123425B/fi
Priority to US15/196,716 priority patent/US10221377B2/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/20Organic compounds containing oxygen
    • C11D3/2003Alcohols; Phenols
    • C11D3/2065Polyhydric alcohols
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10Objects to be cleaned
    • C11D2111/12Soft surfaces, e.g. textile
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10Objects to be cleaned
    • C11D2111/14Hard surfaces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Emergency Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Esillä oleva keksintö kohdistuu sieniperäiseen seriiniproteaasientsyymiin, jolla mainitulla entsyymillä on seriiniproteaasin aktiivisuutta ja jolla on kypsän Malbranchea ALKO4122-proteaasin aminohapposekvenssi, joka on määritelty sekvenssissä SEQ ID NO:18, tai aminohapposekvenssi, jonka samanlaisuus sekvenssin SEQ ID NO:18 mukaisen aminohapposekvenssin kanssa on vähintään 66 %. Samoin esitetään eristetty nukleiinihappomolekyyli, jonka sisältämä polynukleotidisekvenssi koodaa sieniperäistä seriiniproteaasientsyymiä, mainittua proteaasia koodaavat nukleiinihapposekvenssit, isäntäsolu ja menetelmä polypeptidin, jolla on seriiniproteaasin aktiivisuutta, tuottamiseksi. Mainittu proteaasi on käyttökelpoinen entsyymivalmisteena, jota voidaan käyttää pesuainekoostumuksissa ja kuitujen, villan, hiusten/karvojen, nahan ja silkin käsittelyyn, elintarvikkeiden ja eläinravinnon käsittelyyn tai mihin tahansa muihin sellaisiin sovelluksiin, joihin liittyy proteiinipitoisen materiaalin muokkausta, hajotusta tai poistoa.
FI20115310A 2011-03-31 2011-03-31 Proteaasientsyymi ja sen käytöt FI123425B (fi)

Priority Applications (12)

Application Number Priority Date Filing Date Title
FI20115310A FI123425B (fi) 2011-03-31 2011-03-31 Proteaasientsyymi ja sen käytöt
US13/433,984 US9404164B2 (en) 2011-03-31 2012-03-29 Protease enzyme and uses thereof
JP2014501649A JP2014511681A (ja) 2011-03-31 2012-03-30 プロテアーゼ酵素およびその使用
CN201280026496.9A CN103890172B (zh) 2011-03-31 2012-03-30 蛋白酶及其应用
BR112013025238A BR112013025238A2 (pt) 2011-03-31 2012-03-30 enzima serina protease, molécula de ácido nucleico isolada, vetor de expressão recombinante, célula hospedeira, processos para produzir um polipeptídeo com atividade serina protease, e para obter uma preparação enzimática, polipeptídeo com atividade serina protease, preparação enzimática, uso de enzima serina protease, e, composição de detergente
ES12722084.6T ES2585652T3 (es) 2011-03-31 2012-03-30 Enzima proteasa y usos de la misma
EP12722084.6A EP2691520B1 (en) 2011-03-31 2012-03-30 Protease enzyme and uses thereof
KR1020137029011A KR102010747B1 (ko) 2011-03-31 2012-03-30 프로테아제 효소 및 이의 용도
RU2013146665A RU2611043C2 (ru) 2011-03-31 2012-03-30 Фермент протеаза и его применения
DK12722084.6T DK2691520T3 (en) 2011-03-31 2012-03-30 Protease enzyme and uses thereof
PCT/EP2012/055762 WO2012131023A2 (en) 2011-03-31 2012-03-30 Protease enzyme and uses thereof
US15/196,716 US10221377B2 (en) 2011-03-31 2016-06-29 Protease enzyme and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20115310A FI123425B (fi) 2011-03-31 2011-03-31 Proteaasientsyymi ja sen käytöt
FI20115310 2011-03-31

Publications (4)

Publication Number Publication Date
FI20115310A0 FI20115310A0 (fi) 2011-03-31
FI20115310L true FI20115310L (fi) 2012-10-01
FI20115310A FI20115310A (fi) 2012-10-01
FI123425B FI123425B (fi) 2013-04-30

Family

ID=43806518

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20115310A FI123425B (fi) 2011-03-31 2011-03-31 Proteaasientsyymi ja sen käytöt

Country Status (11)

Country Link
US (2) US9404164B2 (fi)
EP (1) EP2691520B1 (fi)
JP (1) JP2014511681A (fi)
KR (1) KR102010747B1 (fi)
CN (1) CN103890172B (fi)
BR (1) BR112013025238A2 (fi)
DK (1) DK2691520T3 (fi)
ES (1) ES2585652T3 (fi)
FI (1) FI123425B (fi)
RU (1) RU2611043C2 (fi)
WO (1) WO2012131023A2 (fi)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
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EP2712915A1 (en) * 2012-10-01 2014-04-02 The Procter and Gamble Company Methods of treating a surface and compositions for use therein
WO2015158719A1 (en) * 2014-04-15 2015-10-22 Aalborg Universitet Composition and method for degradation of keratinaceous materials
EP3645147A1 (en) 2017-06-30 2020-05-06 Diversey, Inc. Membrane cleaning solution and method of accelerated membrane cleaning using the same
GB2569611B (en) * 2017-12-21 2020-02-19 Cordina Ltd Improvements in hair-curling devices
CN112639066B (zh) * 2018-08-27 2023-08-22 巴斯夫欧洲公司 改进去污力的聚合物活性成分
EP3636735B1 (en) * 2018-10-12 2024-03-27 AB Enzymes Oy Protease enzyme variants and uses thereof

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FI121711B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
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Also Published As

Publication number Publication date
KR102010747B1 (ko) 2019-08-14
RU2611043C2 (ru) 2017-02-20
US9404164B2 (en) 2016-08-02
WO2012131023A2 (en) 2012-10-04
US20160376530A1 (en) 2016-12-29
JP2014511681A (ja) 2014-05-19
EP2691520A2 (en) 2014-02-05
DK2691520T3 (en) 2016-08-22
US10221377B2 (en) 2019-03-05
BR112013025238A2 (pt) 2018-09-25
FI123425B (fi) 2013-04-30
KR20140032396A (ko) 2014-03-14
ES2585652T3 (es) 2016-10-07
RU2013146665A (ru) 2015-05-20
CN103890172B (zh) 2015-11-25
US20120252064A1 (en) 2012-10-04
WO2012131023A3 (en) 2012-11-22
FI20115310A (fi) 2012-10-01
CN103890172A (zh) 2014-06-25
FI20115310A0 (fi) 2011-03-31
EP2691520B1 (en) 2016-05-18

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